Gabriela Stiegler

Protection of Macaques against Pathogenic Simian/Human Immunodeficiency Virus 89.6PD by Passive Transfer of Neutralizing Antibodies

Mascola, John R., Lewis, Mark G., Stiegler, Gabriela, Harris, Dawn, VanCott, Thomas C., Hayes, Deborah, ...

The role of antibody in protection against human immunodeficiency virus (HIV-1) has been difficult to study in animal models because most primary HIV-1 strains do not infect nonhuman primates. Using...

Broadly Neutralizing Antibodies Targeted to the Membrane-Proximal External Region of Human Immunodeficiency Virus Type 1 Glycoprotein gp41

Zwick, Michael B., Labrijn, Aran F., Wang, Meng, Spenlehauer, Catherine, Saphire, Erica Ollmann, Binley, James M., ...

The identification and epitope mapping of broadly neutralizing anti-human immunodeficiency virus type 1 (HIV-1) antibodies (Abs) is important for vaccine design, but, despite much effort, very few...

Postnatal Passive Immunization of Neonatal Macaques with a Triple Combination of Human Monoclonal Antibodies against Oral Simian-Human Immunodeficiency Virus Challenge

Hofmann-Lehmann, Regina, Vlasak, Josef, Rasmussen, Robert A., Smith, Beverly A., Baba, Timothy W., Liska, Vladimir, ...

To develop prophylaxis against mother-to-child human immunodeficiency virus (HIV) transmission, we established a simian-human immunodeficiency virus (SHIV) infection model in neonatal macaques that...

Membrane-Anchored Peptide Inhibits Human Immunodeficiency Virus Entry

Hildinger, Markus, Dittmar, Matthias T., Schult-Dietrich, Patricia, Fehse, Boris, Schnierle, Barbara S., Thaler, Sonja, ...

Peptides derived from the heptad repeats of human immunodeficiency virus (HIV) gp41 envelope glycoprotein, such as T20, can efficiently inhibit HIV type 1 (HIV-1) entry. In this study, replication of...

Neutralization Synergy of Human Immunodeficiency Virus Type 1 Primary Isolates by Cocktails of Broadly Neutralizing Antibodies

Zwick, Michael B., Wang, Meng, Poignard, Pascal, Stiegler, Gabriela, Katinger, Hermann, Burton, Dennis R., ...

Several reports have described the existence of synergy between neutralizing monoclonal antibodies (MAbs) against human immunodeficiency virus type 1 (HIV-1). Synergy between human MAbs b12, 2G12,...

Characterization of Human Class-Switched Polymeric (Immunoglobulin M [IgM] and IgA) Anti-Human Immunodeficiency Virus Type 1 Antibodies 2F5 and 2G12

Wolbank, Susanne, Kunert, Renate, Stiegler, Gabriela, Katinger, Hermann

We have previously generated human monoclonal anti-human immunodeficiency virus type 1 (anti-HIV-1) antibodies 2F5IgG and 2G12IgG with an exceptional cross-clade neutralizing potential. 2F5IgG and...

Cellular Immunity Elicited by Human Immunodeficiency Virus Type 1/ Simian Immunodeficiency Virus DNA Vaccination Does Not Augment the Sterile Protection Afforded by Passive Infusion of Neutralizing Antibodies

Mascola, John R., Lewis, Mark G., VanCott, Thomas C., Stiegler, Gabriela, Katinger, Hermann, Seaman, Michael, ...

High levels of infused anti-human immunodeficiency virus type 1 (HIV-1) neutralizing monoclonal antibodies (MAbs) can completely protect macaque monkeys against mucosal chimeric simian-human...

Effects of Antibody on Viral Kinetics in Simian/Human Immunodeficiency Virus Infection: Implications for Vaccination

Zhang, Lei, Ribeiro, Ruy M., Mascola, John R., Lewis, Mark G., Stiegler, Gabriela, Katinger, Hermann, ...

Passive antibody treatment of macaques prior to simian/human immunodeficiency virus infection produces “sterilizing immunity” in some animals and long-term reductions in viral loads in others....

Comprehensive Cross-Clade Neutralization Analysis of a Panel of Anti-Human Immunodeficiency Virus Type 1 Monoclonal Antibodies

Binley, James M., Wrin, Terri, Korber, Bette, Zwick, Michael B., Wang, Meng, Chappey, Colombe, ...

Broadly neutralizing monoclonal antibodies (MAbs) are potentially important tools in human immunodeficiency virus type 1 (HIV-1) vaccine design. A few rare MAbs have been intensively studied, but we...

Neutralization Profiles of Newly Transmitted Human Immunodeficiency Virus Type 1 by Monoclonal Antibodies 2G12, 2F5, and 4E10

Mehandru, Saurabh, Wrin, Terri, Galovich, Justin, Stiegler, Gabriela, Vcelar, Brigitta, Hurley, Arlene, ...

As the AIDS epidemic continues unabated, the development of a human immunodeficiency virus (HIV) vaccine is critical. Ideally, an effective vaccine should elicit cell-mediated and neutralizing...

Anti-Human Immunodeficiency Virus Type 1 (HIV-1) Antibodies 2F5 and 4E10 Require Surprisingly Few Crucial Residues in the Membrane-Proximal External Region of Glycoprotein gp41 To Neutralize HIV-1

Zwick, Michael B., Jensen, Richard, Church, Sarah, Wang, Meng, Stiegler, Gabriela, Kunert, Renate, ...

The conserved membrane-proximal external region (MPER) of human immunodeficiency virus type 1 (HIV-1) gp41 is a target of two broadly neutralizing human monoclonal antibodies, 2F5 and 4E10, and is an...

Virus Isolates during Acute and Chronic Human Immunodeficiency Virus Type 1 Infection Show Distinct Patterns of Sensitivity to Entry Inhibitors

Rusert, Peter, Kuster, Herbert, Joos, Beda, Misselwitz, Benjamin, Gujer, Cornelia, Leemann, Christine, ...

We studied the effect of entry inhibitors on 58 virus isolates derived during acute and chronic infection to validate these inhibitors in vitro and to probe whether viruses at early and chronic...

Generation of an Influenza A Virus Vector Expressing Biologically Active Human Interleukin-2 from the NS Gene Segment

Kittel, Christian, Ferko, Boris, Kurz, Martina, Voglauer, Regina, Sereinig, Sabine, Romanova, Julia, ...

Engineering of the influenza A virus NS1 protein became an attractive approach to the development of influenza vaccine vectors since it can tolerate large inserts of foreign proteins. However,...

Long-Term Multiple-Dose Pharmacokinetics of Human Monoclonal Antibodies (MAbs) against Human Immunodeficiency Virus Type 1 Envelope gp120 (MAb 2G12) and gp41 (MAbs 4E10 and 2F5)

Joos, Beda, Trkola, Alexandra, Kuster, Herbert, Aceto, Leonardo, Fischer, Marek, Stiegler, Gabriela, ...

While certain antibodies directed against the human immunodeficiency virus (HIV) envelope have the potential to suppress virus replication in vitro, the impact of neutralizing antibodies in vivo...

Protection of Macaques against Pathogenic Simian/Human Immunodeficiency Virus 89.6PD by Passive Transfer of Neutralizing Antibodies

Mascola, John R., Lewis, Mark G., Stiegler, Gabriela, Harris, Dawn, VanCott, Thomas C., Hayes, Deborah, ...

The role of antibody in protection against human immunodeficiency virus (HIV-1) has been difficult to study in animal models because most primary HIV-1 strains do not infect nonhuman primates. Using...

Broadly Neutralizing Antibodies Targeted to the Membrane-Proximal External Region of Human Immunodeficiency Virus Type 1 Glycoprotein gp41

Zwick, Michael B., Labrijn, Aran F., Wang, Meng, Spenlehauer, Catherine, Saphire, Erica Ollmann, Binley, James M., ...

The identification and epitope mapping of broadly neutralizing anti-human immunodeficiency virus type 1 (HIV-1) antibodies (Abs) is important for vaccine design, but, despite much effort, very few...

Postnatal Passive Immunization of Neonatal Macaques with a Triple Combination of Human Monoclonal Antibodies against Oral Simian-Human Immunodeficiency Virus Challenge

Hofmann-Lehmann, Regina, Vlasak, Josef, Rasmussen, Robert A., Smith, Beverly A., Baba, Timothy W., Liska, Vladimir, ...

To develop prophylaxis against mother-to-child human immunodeficiency virus (HIV) transmission, we established a simian-human immunodeficiency virus (SHIV) infection model in neonatal macaques that...

Membrane-Anchored Peptide Inhibits Human Immunodeficiency Virus Entry

Hildinger, Markus, Dittmar, Matthias T., Schult-Dietrich, Patricia, Fehse, Boris, Schnierle, Barbara S., Thaler, Sonja, ...

Peptides derived from the heptad repeats of human immunodeficiency virus (HIV) gp41 envelope glycoprotein, such as T20, can efficiently inhibit HIV type 1 (HIV-1) entry. In this study, replication of...

Neutralization Synergy of Human Immunodeficiency Virus Type 1 Primary Isolates by Cocktails of Broadly Neutralizing Antibodies

Zwick, Michael B., Wang, Meng, Poignard, Pascal, Stiegler, Gabriela, Katinger, Hermann, Burton, Dennis R., ...

Several reports have described the existence of synergy between neutralizing monoclonal antibodies (MAbs) against human immunodeficiency virus type 1 (HIV-1). Synergy between human MAbs b12, 2G12,...

Characterization of Human Class-Switched Polymeric (Immunoglobulin M [IgM] and IgA) Anti-Human Immunodeficiency Virus Type 1 Antibodies 2F5 and 2G12

Wolbank, Susanne, Kunert, Renate, Stiegler, Gabriela, Katinger, Hermann

We have previously generated human monoclonal anti-human immunodeficiency virus type 1 (anti-HIV-1) antibodies 2F5IgG and 2G12IgG with an exceptional cross-clade neutralizing potential. 2F5IgG and...

Cellular Immunity Elicited by Human Immunodeficiency Virus Type 1/ Simian Immunodeficiency Virus DNA Vaccination Does Not Augment the Sterile Protection Afforded by Passive Infusion of Neutralizing Antibodies

Mascola, John R., Lewis, Mark G., VanCott, Thomas C., Stiegler, Gabriela, Katinger, Hermann, Seaman, Michael, ...

High levels of infused anti-human immunodeficiency virus type 1 (HIV-1) neutralizing monoclonal antibodies (MAbs) can completely protect macaque monkeys against mucosal chimeric simian-human...

Effects of Antibody on Viral Kinetics in Simian/Human Immunodeficiency Virus Infection: Implications for Vaccination

Zhang, Lei, Ribeiro, Ruy M., Mascola, John R., Lewis, Mark G., Stiegler, Gabriela, Katinger, Hermann, ...

Passive antibody treatment of macaques prior to simian/human immunodeficiency virus infection produces “sterilizing immunity” in some animals and long-term reductions in viral loads in others....

Comprehensive Cross-Clade Neutralization Analysis of a Panel of Anti-Human Immunodeficiency Virus Type 1 Monoclonal Antibodies

Binley, James M., Wrin, Terri, Korber, Bette, Zwick, Michael B., Wang, Meng, Chappey, Colombe, ...

Broadly neutralizing monoclonal antibodies (MAbs) are potentially important tools in human immunodeficiency virus type 1 (HIV-1) vaccine design. A few rare MAbs have been intensively studied, but we...

Neutralization Profiles of Newly Transmitted Human Immunodeficiency Virus Type 1 by Monoclonal Antibodies 2G12, 2F5, and 4E10

Mehandru, Saurabh, Wrin, Terri, Galovich, Justin, Stiegler, Gabriela, Vcelar, Brigitta, Hurley, Arlene, ...

As the AIDS epidemic continues unabated, the development of a human immunodeficiency virus (HIV) vaccine is critical. Ideally, an effective vaccine should elicit cell-mediated and neutralizing...

Anti-Human Immunodeficiency Virus Type 1 (HIV-1) Antibodies 2F5 and 4E10 Require Surprisingly Few Crucial Residues in the Membrane-Proximal External Region of Glycoprotein gp41 To Neutralize HIV-1

Zwick, Michael B., Jensen, Richard, Church, Sarah, Wang, Meng, Stiegler, Gabriela, Kunert, Renate, ...

The conserved membrane-proximal external region (MPER) of human immunodeficiency virus type 1 (HIV-1) gp41 is a target of two broadly neutralizing human monoclonal antibodies, 2F5 and 4E10, and is an...

Virus Isolates during Acute and Chronic Human Immunodeficiency Virus Type 1 Infection Show Distinct Patterns of Sensitivity to Entry Inhibitors

Rusert, Peter, Kuster, Herbert, Joos, Beda, Misselwitz, Benjamin, Gujer, Cornelia, Leemann, Christine, ...

We studied the effect of entry inhibitors on 58 virus isolates derived during acute and chronic infection to validate these inhibitors in vitro and to probe whether viruses at early and chronic...

Generation of an Influenza A Virus Vector Expressing Biologically Active Human Interleukin-2 from the NS Gene Segment

Kittel, Christian, Ferko, Boris, Kurz, Martina, Voglauer, Regina, Sereinig, Sabine, Romanova, Julia, ...

Engineering of the influenza A virus NS1 protein became an attractive approach to the development of influenza vaccine vectors since it can tolerate large inserts of foreign proteins. However,...

Long-Term Multiple-Dose Pharmacokinetics of Human Monoclonal Antibodies (MAbs) against Human Immunodeficiency Virus Type 1 Envelope gp120 (MAb 2G12) and gp41 (MAbs 4E10 and 2F5)

Joos, Beda, Trkola, Alexandra, Kuster, Herbert, Aceto, Leonardo, Fischer, Marek, Stiegler, Gabriela, ...

While certain antibodies directed against the human immunodeficiency virus (HIV) envelope have the potential to suppress virus replication in vitro, the impact of neutralizing antibodies in vivo...

In Vivo and In Vitro Escape from Neutralizing Antibodies 2G12, 2F5, and 4E10▿

Manrique, Amapola, Rusert, Peter, Joos, Beda, Fischer, Marek, Kuster, Herbert, Leemann, Christine, ...

Recently, passive immunization of human immunodeficiency virus (HIV)-infected individuals with monoclonal antibodies (MAbs) 2G12, 2F5, and 4E10 provided evidence of the in vivo activity of 2G12 but...

Adjunctive Passive Immunotherapy in Human Immunodeficiency Virus Type 1-Infected Individuals Treated with Antiviral Therapy during Acute and Early Infection▿ †

Mehandru, Saurabh, Vcelar, Brigitta, Wrin, Terri, Stiegler, Gabriela, Joos, Beda, Mohri, Hiroshi, ...

Three neutralizing monoclonal antibodies (MAbs), 2G12, 2F5, and 4E10, with activity in vitro and in vivo were administered in an open-label, nonrandomized, proof-of-concept study to attempt to...

In Vivo Efficacy of Human Immunodeficiency Virus Neutralizing Antibodies: Estimates for Protective Titers▿

Trkola, Alexandra, Kuster, Herbert, Rusert, Peter, Von Wyl, Viktor, Leemann, Christine, Weber, Rainer, ...

The definition of plasma neutralizing antibody titers capable of controlling human immunodeficiency virus (HIV) infection in vivo is considered a critical step in vaccine development. Here we provide...

Potent Human Immunodeficiency Virus-Neutralizing and Complement Lysis Activities of Antibodies Are Not Obligatorily Linked▿ †

Huber, Michael, Von Wyl, Viktor, Ammann, Christoph G., Kuster, Herbert, Stiegler, Gabriela, Katinger, Hermann, ...

To evaluate the contribution of complement-mediated lysis to the in vivo activities of neutralizing antibodies, we analyzed the influence of complement activation on treatment success in a recent...

Cost-effective production of a vaginal protein microbicide to prevent HIV transmission

Ramessar, Koreen, Rademacher, Thomas, Sack, Markus, Stadlmann, Johannes, Platis, Dimitris, Stiegler, Gabriela, ...

A series of small-molecule microbicides has been developed for vaginal delivery to prevent heterosexual HIV transmission, but results from human clinical trials have been disappointing. Protein-based...