Abstract Background Being sessile organisms, plants should adjust their metabolism to dynamic changes in their environment. Such adjustments need particular coordination in branched metabolic...
Wang, Xuelu, Lopez-Valenzuela, Jose A., Gibbon, Bryan C., Gakiere, Bertrand, Galili, Gad, Larkins, Brian A.
A quantitative trait locus has previously been identified in maize (Zea mays L.) that influences the level of free amino acids in the endosperm, especially those from the aspartate pathway: lysine,...
Sláviková, Silvia, Shy, Galia, Yao, Youli, Glozman, Rina, Levanony, Hanna, Pietrokovski, Shmuel, ...
Arabidopsis plants possess a family of nine AtAtg8 gene homologues of the yeast autophagy-associated Apg8/Aut7 gene. To gain insight into how these genes function in plants, first, the expression...
Sláviková, Silvia, Shy, Galia, Yao, Youli, Glozman, Rina, Levanony, Hanna, Pietrokovski, Shmuel, ...
Arabidopsis plants possess a family of nine AtAtg8 gene homologues of the yeast autophagy-associated Apg8/Aut7 gene. To gain insight into how these genes function in plants, first, the expression...
Sláviková, Silvia, Shy, Galia, Yao, Youli, Glozman, Rina, Levanony, Hanna, Pietrokovski, Shmuel, ...
Arabidopsis plants possess a family of nine AtAtg8 gene homologues of the yeast autophagy-associated Apg8/Aut7 gene. To gain insight into how these genes function in plants, first, the expression...
Yao, Youli, Song, Luhua, Katz, Yael, Galili, Gad
The 3′ cleavage and polyadenylation of mRNAs has been studied in detail in animals and yeast, but not in plants. Aimed at elucidating the regulation of mRNA 3′ end formation in plants, three...
Shy, Galia, Ehler, Linda, Herman, Eliot, Galili, Gad
Wheat storage proteins are deposited in the vacuole of maturing endosperm cells by a novel pathway that is the result of protein body formation by the endoplasmic reticulum followed by autophagy into...
Galili, Gad, Kawata, Evelynn E., Cuellar, Richard E., Smith, L.D., Larkins, Brian A.
A maize zein cDNA clone was used to synthesize mRNA with the SP6 in vitro transcription system. Although we obtained full-length transcripts of the cDNA sequence, these were inefficient templates for...
Galili, Gad, Levy, Abraham, Jakob, Karl M.
Discrete deoxyribonucleoproteins (DNPs) containing nascent and/or bulk DNA, were obtained by fractionating micrococcal nuclease digests of nuclei from 3H-thymidine pulse (15–20 sec) and...
Zhu-Shimoni, Judith X., Galili, Gad
Although the control of carbon fixation and nitrogen assimilation has been studied in detail, relatively little is known about the regulation of carbon and nitrogen flow into amino acids. In this...
Miron, Daphna, Ben-Yaacov, Sari, Reches, Dalit, Schupper, Avigail, Galili, Gad
Both in mammals and plants, excess lysine (Lys) is catabolized via saccharopine into α-amino adipic semialdehyde and glutamate by two consecutive enzymes, Lys-ketoglutarate reductase (LKR) and...
Zhu, Xiaohong, Tang, Guiliang, Galili, Gad
Arabidopsis plants possess a composite AtLKR/SDH locus encoding two different polypeptides involved in lysine catabolism: a bifunctional lysine-ketoglutarate reductase/saccharopine dehydrogenase...
Zhu, Xiaohong, Tang, Guiliang, Granier, Fabienne, Bouchez, David, Galili, Gad
Plants possess both anabolic and catabolic pathways for the essential amino acid lysine (Lys). However, although the biosynthetic pathway was clearly shown to regulate Lys accumulation in plants, the...
Aharon, Refael, Shahak, Yosepha, Wininger, Smadar, Bendov, Rozalina, Kapulnik, Yoram, Galili, Gad
Most of the symplastic water transport in plants occurs via aquaporins, but the extent to which aquaporins contribute to plant water status under favorable growth conditions and abiotic stress is not...
To elucidate the relative significance of Lys synthesis and catabolism in determining Lys level in plant seeds, we expressed a bacterial feedback-insensitive dihydrodipicolinate synthase (DHPS) in a...
Tang, Guiliang, Zhu, Xiaohong, Gakiere, Bertrand, Levanony, Hanna, Kahana, Anat, Galili, Gad
Both plants and animals catabolize lysine (Lys) via two consecutive enzymes, Lys-ketoglutarate reductase (LKR) and saccharopine dehydrogenase (SDH), which are linked on a single polypeptide encoded...
In plants, excess cellular lysine (Lys) is catabolized into glutamic acid and acetyl-coenzyme A; yet, it is still not clear whether this pathway has other functions in addition to balancing Lys...
Gene-dosage compensation of endosperm proteins in hexaploid wheat Triticum aestivum
Galili, Gad, Levy, Avraham A., Feldman, Moshe
Several aneuploid lines and one intervarietal substitution line of the hexaploid wheat Triticum aestivum (2n = 6x = 42; genomes AABBDD) cv. Chinese Spring were used to study the effects of different...
The functional role of Lys catabolism in balancing Lys levels in plants has only been directly demonstrated in developing seeds. Seed-specific expression of a bacterial feedback-insensitive...
Expression of a Maize Storage Protein Gene in Petunia Plants Is Not Restricted to Seeds 1
Ueng, Peter, Galili, Gad, Sapanara, Victoria, Goldsbrough, Peter B., Dube, Phillip, Beachy, Roger N., ...
Genes encoding maize seed storage proteins, zeins, are expressed in developing endosperm tissue. To determine whether the DNA sequences controlling the developmental expression of these genes are...
The Synthesis of a 19 Kilodalton Zein Protein in Transgenic Petunia Plants 1
Williamson, John D., Galili, Gad, Larkins, Brian A., Gelvin, Stanton B.
Transcriptional fusions composed of a 19 kilodalton zein cDNA, the 5′ flanking region from a β-phaseolin gene, and 3′ flanking regions from either the phaseolin or a 15-kilodalton zein gene were...
Characterization of Polypeptides Corresponding to Clones of Maize Zein mRNAS
Galili, Gad, Kawata, Evelynn E., Larkins, Brian A.
Zeins, the storage proteins of maize (Zea mays) are a complex group of polypeptides encoded by a large multigene family. The α-zein proteins, which account for about 70% of the total, show both size...
In higher plants, the synthesis of the essential amino acid threonine is regulated primarily by the sensitivity of the first enzyme in its biosynthetic pathway, aspartate kinase, to feedback...
Shani, Noam, Steffen-Campbell, Jody D., Anderson, Olin D., Greene, Frank C., Galili, Gad
The high molecular weight glutenin subunits are considered one of the most important components of wheat (Triticum aestivum) gluten, but their structure and interactions with other gluten proteins...
Evidence for the Presence of Two Different Types of Protein Bodies in Wheat Endosperm
Rubin, Regina, Levanony, Hanna, Galili, Gad
Storage proteins of wheat grains (Triticum L. em Thell) are deposited in protein bodies inside vacuoles. However, the subcellular sites and mechanisms of their aggregation into protein bodies are not...
Zhu-Shimoni, Judith X., Galili, Gad
Although the control of carbon fixation and nitrogen assimilation has been studied in detail, relatively little is known about the regulation of carbon and nitrogen flow into amino acids. In this...
Miron, Daphna, Ben-Yaacov, Sari, Reches, Dalit, Schupper, Avigail, Galili, Gad
Both in mammals and plants, excess lysine (Lys) is catabolized via saccharopine into α-amino adipic semialdehyde and glutamate by two consecutive enzymes, Lys-ketoglutarate reductase (LKR) and...
Zhu, Xiaohong, Tang, Guiliang, Galili, Gad
Arabidopsis plants possess a composite AtLKR/SDH locus encoding two different polypeptides involved in lysine catabolism: a bifunctional lysine-ketoglutarate reductase/saccharopine dehydrogenase...
Zhu, Xiaohong, Tang, Guiliang, Granier, Fabienne, Bouchez, David, Galili, Gad
Plants possess both anabolic and catabolic pathways for the essential amino acid lysine (Lys). However, although the biosynthetic pathway was clearly shown to regulate Lys accumulation in plants, the...
Aharon, Refael, Shahak, Yosepha, Wininger, Smadar, Bendov, Rozalina, Kapulnik, Yoram, Galili, Gad
Most of the symplastic water transport in plants occurs via aquaporins, but the extent to which aquaporins contribute to plant water status under favorable growth conditions and abiotic stress is not...
To elucidate the relative significance of Lys synthesis and catabolism in determining Lys level in plant seeds, we expressed a bacterial feedback-insensitive dihydrodipicolinate synthase (DHPS) in a...
Tang, Guiliang, Zhu, Xiaohong, Gakiere, Bertrand, Levanony, Hanna, Kahana, Anat, Galili, Gad
Both plants and animals catabolize lysine (Lys) via two consecutive enzymes, Lys-ketoglutarate reductase (LKR) and saccharopine dehydrogenase (SDH), which are linked on a single polypeptide encoded...
In plants, excess cellular lysine (Lys) is catabolized into glutamic acid and acetyl-coenzyme A; yet, it is still not clear whether this pathway has other functions in addition to balancing Lys...
Gene-dosage compensation of endosperm proteins in hexaploid wheat Triticum aestivum
Galili, Gad, Levy, Avraham A., Feldman, Moshe
Several aneuploid lines and one intervarietal substitution line of the hexaploid wheat Triticum aestivum (2n = 6x = 42; genomes AABBDD) cv. Chinese Spring were used to study the effects of different...
The functional role of Lys catabolism in balancing Lys levels in plants has only been directly demonstrated in developing seeds. Seed-specific expression of a bacterial feedback-insensitive...
Expression of a Maize Storage Protein Gene in Petunia Plants Is Not Restricted to Seeds 1
Ueng, Peter, Galili, Gad, Sapanara, Victoria, Goldsbrough, Peter B., Dube, Phillip, Beachy, Roger N., ...
Genes encoding maize seed storage proteins, zeins, are expressed in developing endosperm tissue. To determine whether the DNA sequences controlling the developmental expression of these genes are...
The Synthesis of a 19 Kilodalton Zein Protein in Transgenic Petunia Plants 1
Williamson, John D., Galili, Gad, Larkins, Brian A., Gelvin, Stanton B.
Transcriptional fusions composed of a 19 kilodalton zein cDNA, the 5′ flanking region from a β-phaseolin gene, and 3′ flanking regions from either the phaseolin or a 15-kilodalton zein gene were...
Characterization of Polypeptides Corresponding to Clones of Maize Zein mRNAS
Galili, Gad, Kawata, Evelynn E., Larkins, Brian A.
Zeins, the storage proteins of maize (Zea mays) are a complex group of polypeptides encoded by a large multigene family. The α-zein proteins, which account for about 70% of the total, show both size...
In higher plants, the synthesis of the essential amino acid threonine is regulated primarily by the sensitivity of the first enzyme in its biosynthetic pathway, aspartate kinase, to feedback...
Shani, Noam, Steffen-Campbell, Jody D., Anderson, Olin D., Greene, Frank C., Galili, Gad
The high molecular weight glutenin subunits are considered one of the most important components of wheat (Triticum aestivum) gluten, but their structure and interactions with other gluten proteins...
Evidence for the Presence of Two Different Types of Protein Bodies in Wheat Endosperm
Rubin, Regina, Levanony, Hanna, Galili, Gad
Storage proteins of wheat grains (Triticum L. em Thell) are deposited in protein bodies inside vacuoles. However, the subcellular sites and mechanisms of their aggregation into protein bodies are not...
Fait, Aaron, Angelovici, Ruthie, Less, Hadar, Ohad, Itzhak, Urbanczyk-Wochniak, Ewa, Fernie, Alisdair R., ...
While the metabolic networks in developing seeds during the period of reserve accumulation have been extensively characterized, much less is known about those present during seed desiccation and...
Slavikova, Silvia, Ufaz, Shai, Avin-Wittenberg, Tamar, Levanony, Hanna, Galili, Gad
Eukaryotes contain a ubiquitous family of autophagy-associated Atg8 proteins. In animal cells, these proteins have multiple functions associated with growth, cancer, and degenerative diseases, but...
Using a bioinformatics analysis of public Arabidopsis (Arabidopsis thaliana) microarray data, we propose here a novel regulatory program, combining transcriptional and posttranslational controls,...