Gaetano T. Montelione

A Topology-Constrained Distance Network Algorithm for Protein Structure Determination From NOESY Data (2009)

Yuanpeng Janet Huang, Roberto Tejero, Robert Powers, Gaetano T. Montelione

ABSTRACT This article formulates the multidimensional nuclear Overhauser effect spectroscopy (NOESY) interpretation problem using graph theory and presents a novel, bottom-up, topology-constrained...

A Topology-Constrained Distance Network Algorithm for Protein Structure Determination From NOESY Data (2008)

Yuanpeng Janet Huang, Roberto Tejero, Robert Powers, Gaetano T. Montelione

ABSTRACT This article formulates the multidimensional nuclear Overhauser effect spectroscopy (NOESY) interpretation problem using graph theory and presents a novel, bottom-up, topology-constrained...

The solution structure of ribosomal protein S17E from Methanobacterium thermoautotrophicum: A structural homolog of the FF domain (2008)

Wu, Bin, Yee, Adelinda, Huang, Yuanpeng J., Ramelot, Theresa A., Cort, John R., Semesi, Anthony, ...

The ribosomal protein S17E from the archaeon Methanobacterium thermoautotrophicum is a component of the 30S ribosomal subunit. S17E is a 62-residue protein conserved in archaea and eukaryotes and has...

Comparing Classical Pathways and Modern Networks: Towards the Development of an Edge Ontology (2007)

Lu, Long J., Sboner, Andrea, Huang, Yuanpeng J., Lu, Hao Xin, Gianoulis, Tara A., Yip, Kevin Y., ...

Pathways are integral to systems biology. Their classical representation has proven useful but is inconsistent in the meaning assigned to each arrow (or edge) and inadvertently implies the isolation...

Crystal structure of AGR_C_4470p from Agrobacterium tumefaciens (2007)

Vorobiev, Sergey M., Neely, Helen, Seetharaman, Jayaraman, Ma, Li-Chung, Xiao, Rong, Acton, Thomas B., ...

We report here the crystal structure at 2.0 Å resolution of the AGR_C_4470p protein from the Gram-negative bacterium Agrobacterium tumefaciens. The protein is a tightly associated dimer, each...

A topology-constrained distance network algorithm for protein structure determination from NOESY data (2006)

Huang, Yuanpeng Janet, Tejero, Roberto, Powers, Robert, Montelione, Gaetano T

This article formulates the multidimensional nuclear Overhauser effect spectroscopy (NOESY) interpretation problem using graph theory and presents a novel, bottom-up, topology-constrained distance...

PubNet: a flexible system for visualizing literature derived networks (2005)

Douglas, Shawn M, Montelione, Gaetano T, Gerstein, Mark

Abstract We have developed PubNet, a web-based tool that extracts several types of relationships returned by PubMed queries and maps them into networks, allowing for graphical visualization, textual...

Solution structure of Archaeglobus fulgidis peptidyl-tRNA hydrolase (Pth2) provides evidence for an extensive conserved family of Pth2 enzymes in archea, bacteria, and eukaryotes (2005)

Powers, Robert, Mirkovic, Nebojsa, Goldsmith-Fischman, Sharon, Acton, Thomas B., Chiang, Yi-wen, Huang, Yuanpeng J., ...

The solution structure of protein AF2095 from the thermophilic archaea Archaeglobus fulgidis, a 123- residue (13.6-kDa) protein, has been determined by NMR methods. The structure of AF2095 is...

Solution structure of Archaeglobus fulgidis peptidyl-tRNA hydrolase (Pth2) provides evidence for an extensive conserved family of Pth2 enzymes in archea, bacteria, and eukaryotes (2005)

Powers, Robert, Mirkovic, Nebojsa, Goldsmith-Fischman, Sharon, Acton, Thomas B., Chiang, Yiwen, Huang, Yuanpeng J., ...

The solution structure of protein AF2095 from the thermophilic archaea Archaeglobus fulgidis, a 123-residue (13.6-kDa) protein, has been determined by NMR methods. The structure of AF2095 is...

High-quality homology models derived from NMR and X-ray structures of E. coli proteins YgdK and Suf E suggest that all members of the YgdK/Suf E protein family are enhancers of cysteine desulfurases (2005)

Liu, Gaohua, Li, Zhaohui, Chiang, Yiwen, Acton, Thomas, Montelione, Gaetano T., Murray, Diana, ...

The structural biology of proteins mediating iron-sulfur (Fe-S) cluster assembly is central for understanding several important biological processes. Here we present the NMR structure of the 16-kDa...

1H, 13C, and 15N assignments for the Archaeglobus fulgidis protein AF2095 (2004)

Powers, Robert, Acton, Thomas B., Chiang, Yi-wen, Rajan, P. K., Cort, John R., Kennedy, Michael A., ...

Structural genomics is providing a means to determine the molecular and cellular function for the vast amount of proteins in the Human proteome that lack any explicit experimental information by...

Validation of helical tilt angles in the solution NMR structure of the Z domain of Staphylococcal protein A by combined analysis of residual dipolar coupling and NOE data (2004)

Zheng, Deyou, Aramini, James M., Montelione, Gaetano T.

Staphylococcal protein A (SpA) is a virulence factor from Staphylococcus aureus that is able to bind to immunoglobulins. The 3D structures of its immunoglobulin (Ig) binding domains have been...

The NMR solution structure of the 30S ribosomal protein S27e encoded in gene RS27_ARCFU of Archaeoglobus fulgidis reveals a novel protein fold (2004)

Herve Du Penhoat, Catherine, Atreya, Hanudatta S., Shen, Yang, Liu, Gaohua, Acton, Thomas B., Xiao, Rong, ...

The Archaeoglobus fulgidis gene RS27_ARCFU encodes the 30S ribosomal protein S27e. Here, we present the high-quality NMR solution structure of this archaeal protein, which comprises a C4 zinc finger...

Validation of helical tilt angles in the solution NMR structure of the Z domain of Staphylococcal protein A by combined analysis of residual dipolar coupling and NOE data (2004)

ZHENG, DEYOU, ARAMINI, JAMES M., MONTELIONE, GAETANO T.

Staphylococcal protein A (SpA) is a virulence factor from Staphylococcus aureus that is able to bind to immunoglobulins. The 3D structures of its immunoglobulin (Ig) binding domains have been...

Automated protein fold determination using a minimal NMR constraint strategy (2003)

Zheng, Deyou, Huang, Yuanpeng J., Moseley, Hunter N.B., Xiao, Rong, Aramini, James, Swapna, G.V.T., ...

Determination of precise and accurate protein structures by NMR generally requires weeks or even months to acquire and interpret all the necessary NMR data. However, even medium-accuracy fold...

SPINE 2: a system for collaborative structural proteomics within a federated database framework (2003)

Goh, Chern-Sing, Lan, Ning, Echols, Nathaniel, Douglas, Shawn M., Milburn, Duncan, Bertone, Paul, ...

We present version 2 of the SPINE system for structural proteomics. SPINE is available over the web at http://nesg.org. It serves as the central hub for the Northeast Structural Genomics Consortium,...

Solution NMR structure of the 30S ribosomal protein S28E from Pyrococcus horikoshii (2003)

Aramini, James M., Huang, Yuanpeng J., Cort, John R., Goldsmith-Fischman, Sharon, Xiao, Rong, Shih, Liang-Yu, ...

We report NMR assignments and solution structure of the 71-residue 30S ribosomal protein S28E from the archaean Pyrococcus horikoshii, target JR19 of the Northeast Structural Genomics Consortium. The...

SPINE: an integrated tracking database and data mining approach for identifying feasible targets in high-throughput structural proteomics (2001)

Bertone, Paul, Kluger, Yuval, Lan, Ning, Zheng, Deyou, Christendat, Dinesh, Yee, Adelinda, ...

High-throughput structural proteomics is expected to generate considerable amounts of data on the progress of structure determination for many proteins. For each protein this includes information...

The role of asparagine-32 in forming the receptor-binding epitope of human epidermal growth factor (1993)

Campion, Stephen R., Biamonti, Clelia, Montelione, Gaetano T., Niyogi, Salil K.

The highly conserved asparagine residue at position 32 (Asn32) in the 'hinge' region of epidermal growth factor (EGF) separates the N- and C-terminal structural motifs of the EGF molecule and is...

SPINE: an integrated tracking database and data mining approach for identifying feasible targets in high-throughput structural proteomics

Bertone, Paul, Kluger, Yuval, Lan, Ning, Zheng, Deyou, Christendat, Dinesh, Yee, Adelinda, ...

High-throughput structural proteomics is expected to generate considerable amounts of data on the progress of structure determination for many proteins. For each protein this includes information...

Reduced-dimensionality NMR spectroscopy for high-throughput protein resonance assignment

Szyperski, Thomas, Yeh, Deok C., Sukumaran, Dinesh K., Moseley, Hunter N. B., Montelione, Gaetano T.

A suite of reduced-dimensionality 13C,15N,1H-triple-resonance NMR experiments is presented for rapid and complete protein resonance assignment. Even when using short measurement times, these...

SPINE 2: a system for collaborative structural proteomics within a federated database framework

Goh, Chern-Sing, Lan, Ning, Echols, Nathaniel, Douglas, Shawn M., Milburn, Duncan, Bertone, Paul, ...

We present version 2 of the SPINE system for structural proteomics. SPINE is available over the web at http://nesg.org. It serves as the central hub for the Northeast Structural Genomics Consortium,...

Crystal structure of RlmAI: Implications for understanding the 23S rRNA G745/G748-methylation at the macrolide antibiotic-binding site

Das, Kalyan, Acton, Thomas, Chiang, Yiwen, Shih, Lydia, Arnold, Eddy, Montelione, Gaetano T.

The RlmA class of enzymes (RlmAI and RlmAII) catalyzes N1-methylation of a guanine base (G745 in Gram-negative and G748 in Gram-positive bacteria) of hairpin 35 of 23S rRNA. We have determined the...

Structural and biochemical studies identify tobacco SABP2 as a methyl salicylate esterase and implicate it in plant innate immunity

Forouhar, Farhad, Yang, Yue, Kumar, Dhirendra, Chen, Yang, Fridman, Eyal, Park, Sang Wook, ...

Salicylic acid (SA) is a critical signal for the activation of plant defense responses against pathogen infections. We recently identified SA-binding protein 2 (SABP2) from tobacco as a protein that...

NMR data collection and analysis protocol for high-throughput protein structure determination

Liu, Gaohua, Shen, Yang, Atreya, Hanudatta S., Parish, David, Shao, Ying, Sukumaran, Dinesh K., ...

A standardized protocol enabling rapid NMR data collection for high-quality protein structure determination is presented that allows one to capitalize on high spectrometer sensitivity: a set of five...

PubNet: a flexible system for visualizing literature derived networks

Douglas, Shawn M, Montelione, Gaetano T, Gerstein, Mark

PubNet is a web-based tool to extract several types of relationships returned by PubMed queries and map them into networks.

SPINE: an integrated tracking database and data mining approach for identifying feasible targets in high-throughput structural proteomics

Bertone, Paul, Kluger, Yuval, Lan, Ning, Zheng, Deyou, Christendat, Dinesh, Yee, Adelinda, ...

High-throughput structural proteomics is expected to generate considerable amounts of data on the progress of structure determination for many proteins. For each protein this includes information...

Reduced-dimensionality NMR spectroscopy for high-throughput protein resonance assignment

Szyperski, Thomas, Yeh, Deok C., Sukumaran, Dinesh K., Moseley, Hunter N. B., Montelione, Gaetano T.

A suite of reduced-dimensionality 13C,15N,1H-triple-resonance NMR experiments is presented for rapid and complete protein resonance assignment. Even when using short measurement times, these...

SPINE 2: a system for collaborative structural proteomics within a federated database framework

Goh, Chern-Sing, Lan, Ning, Echols, Nathaniel, Douglas, Shawn M., Milburn, Duncan, Bertone, Paul, ...

We present version 2 of the SPINE system for structural proteomics. SPINE is available over the web at http://nesg.org. It serves as the central hub for the Northeast Structural Genomics Consortium,...

Crystal structure of RlmAI: Implications for understanding the 23S rRNA G745/G748-methylation at the macrolide antibiotic-binding site

Das, Kalyan, Acton, Thomas, Chiang, Yiwen, Shih, Lydia, Arnold, Eddy, Montelione, Gaetano T.

The RlmA class of enzymes (RlmAI and RlmAII) catalyzes N1-methylation of a guanine base (G745 in Gram-negative and G748 in Gram-positive bacteria) of hairpin 35 of 23S rRNA. We have determined the...

Structural and biochemical studies identify tobacco SABP2 as a methyl salicylate esterase and implicate it in plant innate immunity

Forouhar, Farhad, Yang, Yue, Kumar, Dhirendra, Chen, Yang, Fridman, Eyal, Park, Sang Wook, ...

Salicylic acid (SA) is a critical signal for the activation of plant defense responses against pathogen infections. We recently identified SA-binding protein 2 (SABP2) from tobacco as a protein that...

NMR data collection and analysis protocol for high-throughput protein structure determination

Liu, Gaohua, Shen, Yang, Atreya, Hanudatta S., Parish, David, Shao, Ying, Sukumaran, Dinesh K., ...

A standardized protocol enabling rapid NMR data collection for high-quality protein structure determination is presented that allows one to capitalize on high spectrometer sensitivity: a set of five...

PubNet: a flexible system for visualizing literature derived networks

Douglas, Shawn M, Montelione, Gaetano T, Gerstein, Mark

PubNet is a web-based tool to extract several types of relationships returned by PubMed queries and map them into networks.

Molecular insights into substrate recognition and catalysis by tryptophan 2,3-dioxygenase

Forouhar, Farhad, Anderson, J. L. Ross, Mowat, Christopher G., Vorobiev, Sergey M., Hussain, Arif, Abashidze, Mariam, ...

Tryptophan 2,3-dioxygenase (TDO) and indoleamine 2,3-dioxygenase (IDO) constitute an important, yet relatively poorly understood, family of heme-containing enzymes. Here, we report extensive...

Crystal structure of AGR_C_4470p from Agrobacterium tumefaciens

Vorobiev, Sergey M., Neely, Helen, Seetharaman, Jayaraman, Ma, Li-Chung, Xiao, Rong, Acton, Thomas B., ...

We report here the crystal structure at 2.0 Å resolution of the AGR_C_4470p protein from the Gram-negative bacterium Agrobacterium tumefaciens. The protein is a tightly associated dimer, each...

Solution structure of Archaeglobus fulgidis peptidyl-tRNA hydrolase (Pth2) provides evidence for an extensive conserved family of Pth2 enzymes in archea, bacteria, and eukaryotes

Powers, Robert, Mirkovic, Nebojsa, Goldsmith-Fischman, Sharon, Acton, Thomas B., Chiang, Yiwen, Huang, Yuanpeng J., ...

The solution structure of protein AF2095 from the thermophilic archaea Archaeglobus fulgidis, a 123-residue (13.6-kDa) protein, has been determined by NMR methods. The structure of AF2095 is...

High-quality homology models derived from NMR and X-ray structures of E. coli proteins YgdK and Suf E suggest that all members of the YgdK/Suf E protein family are enhancers of cysteine desulfurases

Liu, Gaohua, Li, Zhaohui, Chiang, Yiwen, Acton, Thomas, Montelione, Gaetano T., Murray, Diana, ...

The structural biology of proteins mediating iron-sulfur (Fe-S) cluster assembly is central for understanding several important biological processes. Here we present the NMR structure of the 16-kDa...

Validation of helical tilt angles in the solution NMR structure of the Z domain of Staphylococcal protein A by combined analysis of residual dipolar coupling and NOE data

Zheng, Deyou, Aramini, James M., Montelione, Gaetano T.

Staphylococcal protein A (SpA) is a virulence factor from Staphylococcus aureus that is able to bind to immunoglobulins. The 3D structures of its immunoglobulin (Ig) binding domains have been...

The NMR solution structure of the 30S ribosomal protein S27e encoded in gene RS27_ARCFU of Archaeoglobus fulgidis reveals a novel protein fold

Herve Du Penhoat, Catherine, Atreya, Hanudatta S., Shen, Yang, Liu, Gaohua, Acton, Thomas B., Xiao, Rong, ...

The Archaeoglobus fulgidis gene RS27_ARCFU encodes the 30S ribosomal protein S27e. Here, we present the high-quality NMR solution structure of this archaeal protein, which comprises a C4 zinc finger...

Automated protein fold determination using a minimal NMR constraint strategy

Zheng, Deyou, Huang, Yuanpeng J., Moseley, Hunter N.B., Xiao, Rong, Aramini, James, Swapna, G.V.T., ...

Determination of precise and accurate protein structures by NMR generally requires weeks or even months to acquire and interpret all the necessary NMR data. However, even medium-accuracy fold...

Solution NMR structure of the 30S ribosomal protein S28E from Pyrococcus horikoshii

Aramini, James M., Huang, Yuanpeng J., Cort, John R., Goldsmith-Fischman, Sharon, Xiao, Rong, Shih, Liang-Yu, ...

We report NMR assignments and solution structure of the 71-residue 30S ribosomal protein S28E from the archaean Pyrococcus horikoshii, target JR19 of the Northeast Structural Genomics Consortium. The...

Consistent blind protein structure generation from NMR chemical shift data

Shen, Yang, Lange, Oliver, Delaglio, Frank, Rossi, Paolo, Aramini, James M., Liu, Gaohua, ...

Protein NMR chemical shifts are highly sensitive to local structure. A robust protocol is described that exploits this relation for de novo protein structure generation, using as input experimental...

The solution structure of ribosomal protein S17E from Methanobacterium thermoautotrophicum: A structural homolog of the FF domain

Wu, Bin, Yee, Adelinda, Huang, Yuanpeng J., Ramelot, Theresa A., Cort, John R., Semesi, Anthony, ...

The ribosomal protein S17E from the archaeon Methanobacterium thermoautotrophicum is a component of the 30S ribosomal subunit. S17E is a 62-residue protein conserved in archaea and eukaryotes and has...

Structural basis for suppression of a host antiviral response by influenza A virus

Das, Kalyan, Ma, Li-Chung, Xiao, Rong, Radvansky, Brian, Aramini, James, Zhao, Li, ...

Influenza A viruses are responsible for seasonal epidemics and high mortality pandemics. A major function of the viral NS1A protein, a virulence factor, is the inhibition of the production of IFN-β...

Quantum chemical 13Cα chemical shift calculations for protein NMR structure determination, refinement, and validation

Vila, Jorge A., Aramini, James M., Rossi, Paolo, Kuzin, Alexandre, Su, Min, Seetharaman, Jayaraman, ...

A recently determined set of 20 NMR-derived conformations of a 48-residue all-α-helical protein, (PDB ID code 2JVD), is validated here by comparing the observed 13Cα chemical shifts with those...

Molecular Insights into the Biosynthesis of the F420 Coenzyme*

Forouhar, Farhad, Abashidze, Mariam, Xu, Huimin, Grochowski, Laura L., Seetharaman, Jayaraman, Hussain, Munif, ...

Coenzyme F420, a hydride carrier, is found in Archaea and some bacteria and has crucial roles in methanogenesis, antibiotic biosynthesis, DNA repair, and activation of antitubercular compounds. CofD,...

Unique opportunities for NMR methods in structural genomics

Montelione, Gaetano T., Arrowsmith, Cheryl, Girvin, Mark E., Kennedy, Michael A., Markley, John L., Powers, Robert, ...

This Perspective, arising from a workshop held in July 2008 in Buffalo NY, provides an overview of the role NMR has played in the United States Protein Structure Initiative (PSI), and a vision of how...

Structural genomics is the largest contributor of novel structural leverage

Nair, Rajesh, Liu, Jinfeng, Soong, Ta-Tsen, Acton, Thomas B., Everett, John K., Kouranov, Andrei, ...

The Protein Structural Initiative (PSI) at the US National Institutes of Health (NIH) is funding four large-scale centers for structural genomics (SG). These centers systematically target many large...

Targeting the Human Cancer Pathway Protein Interaction Network by Structural Genomics*

Huang, Yuanpeng Janet, Hang, Dehua, Lu, Long Jason, Tong, Liang, Gerstein, Mark B., Montelione, Gaetano T.

Structural genomics provides an important approach for characterizing and understanding systems biology. As a step toward better integrating protein three-dimensional (3D) structural information in...