Treede, Irina, Braun, Annika, Jeliaskova, Petia, Giese, Thomas, Füllekrug, Joachim, Griffiths, Gareth, ...
Abstract Background Phosphatidylcholine (PC) is a major lipid of the gastrointestinal mucus layer. We recently showed that mucus from patients suffering from ulcerative colitis has low levels of PC....
Modelling phagosomal lipid networks that regulate actin assembly (2008)
Kühnel, Mark, Mayorga, Luis S, Dandekar, Thomas, Thakar, Juilee, Schwarz, Roland, Anes, Elsa, ...
Abstract Background When purified phagosomes are incubated in the presence of actin under appropriate conditions, microfilaments start growing from the membrane in a process that is affected by ATP...
3D-Web Page Usability Issues; Present and Future (2007)
Steven Kerr, Gareth Griffiths, Victor Bayon
With the global explosion of the Internet, designers have been looking at new and exciting ways that we can interact with what we see. Gradually pages are losing the "Text Only" style and...
3d-web page usability issues; present and future (2007)
Steven Kerr, Gareth Griffiths, Victor Bayon
With the global explosion of the Internet, designers have been looking at new and exciting ways that we can interact with what we see. Gradually pages are losing the “Text Only ” style and...
Integrated network reconstruction, visualization and analysis using YANAsquare (2007)
Schwarz, Roland, Liang, Chunguang, Kaleta, Christoph, Kühnel, Mark, Hoffmann, Eik, Kuznetsov, Sergei, ...
Abstract Background Modeling of metabolic networks includes tasks such as network assembly, network overview, calculation of metabolic fluxes and testing the robustness of the network. Results...
Phagosome proteomes open the way to a better understanding of phagosome function (2007)
Griffiths, Gareth, Mayorga, Luis
Abstract Phagocytic cells take up microbes and other particles into membrane-bounded organelles called phagosomes. Studies on the protein and lipid composition of model phagosomes containing latex...
Chunguang Liang, Christoph Kaleta, Mark Kühnel, Eik Hoffmann, Sergei Kuznetsov, Michael Hecker, ...
© 2007 Schwarz et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License
Disruption of Toxoplasma gondii Parasitophorous Vacuoles by the Mouse p47-Resistance GTPases (2005)
Sascha Martens, Iana Parvanova, Jens Zerrahn, Gareth Griffiths, Gudrun Schell, Gaby Reichmann, ...
The p47 GTPases are essential for interferon-γ-induced cell-autonomous immunity against the protozoan parasite, Toxoplasma gondii, in mice, but the mechanism of resistance is poorly understood. We...
Disruption of Toxoplasma gondii Parasitophorous Vacuoles by the Mouse p47-Resistance GTPases (2005)
Martens, Sascha, Parvanova, Iana, Zerrahn, Jens, Griffiths, Gareth, Schell, Gudrun, Reichmann, Gaby, ...
The p47 GTPases are essential for interferon-γ-induced cell-autonomous immunity against the protozoan parasite, Toxoplasma gondii, in mice, but the mechanism of resistance is poorly understood. We...
A role for the small GTPase Rab21 in the early endocytic pathway (2004)
Simpson, Jeremy C., Griffiths, Gareth, Wessling-Resnick, Marianne, Fransen, Jack A. M., Bennett, Holly, Jones, Arwyn Tomos
Rab proteins comprise a family of monomeric GTPases that control cellular membrane traffic. Rab21 is a poorly characterised member with no known function. Human Rab21 cDNA from K562 cells was...
A role for the small GTPase Rab21 in the early endocytic pathway (2004)
Simpson, Jeremy C., Griffiths, Gareth, Wessling-Resnick, Marianne, Fransen, Jack A. M., Bennett, Holly, Jones, Arwyn Tomos
Rab proteins comprise a family of monomeric GTPases that control cellular membrane traffic. Rab21 is a poorly characterised member with no known function. Human Rab21 cDNA from K562 cells was...
Kjeken, Rune, Egeberg, Morten, Habermann, Anja, Kuehnel, Mark, Peyron, Pascale, Floetenmeyer, Matthias, ...
Actin is implicated in membrane fusion, but the precise mechanisms remain unclear. We showed earlier that membrane organelles catalyze the de novo assembly of F-actin that then facilitates the fusion...
Kjeken, Rune, Egeberg, Morten, Habermann, Anja, Kuehnel, Mark, Peyron, Pascale, Floetenmeyer, Matthias, ...
Actin is implicated in membrane fusion, but the precise mechanisms remain unclear. We showed earlier that membrane organelles catalyze the de novo assembly of F-actin that then facilitates the fusion...
Towards an e-commerce business strategy (2002)
Hackney, Ray A., Griffiths, Gareth, Ranchhod, Ashok
Full-text of this article is not available in this e-prints service. This article was originally published following peer-review in International Journal of Services Technology and Management,...
Towards an e-commerce business strategy (2002)
Hackney, Ray A., Griffiths, Gareth, Ranchhod, Ashok
Full-text of this article is not available in this e-prints service. This article was originally published following peer-review in International Journal of Services Technology and Management,...
Wagstaff, Carol, Leverentz, Michael K., Griffiths, Gareth, Thomas, Brian, Chanasut, Usawadee, Stead, Anthony D., ...
The functional life of the flower is terminated by senescence and/&!sol;or abscission. Multiple processes contribute to produce the visible signs of petal wilting and inrolling that typify...
Human cofilin forms oligomers exhibiting actin bundling activity (2001)
Pfannstiel,Jens, Cyrklaff,Marek, Habermann,Anja, Stoeva,Stanka, Griffiths,Gareth, Shoeman,Robert, ...
Human cofilin possesses the tendency for self-association, as indicated by the rapid formation of dimers and oligomers when reacted with water-soluble carbodiimide, Ellmans reagent, or glutathione...
High Temporal Resolution XMM Monitoring of PKS 2155-304 (2001)
Edelson, Rick, Griffiths, Gareth, Markowitz, Alex, Sembay, Steve, Turner, Martin J. L., Warwick, Robert
The bright, strongly variable BL Lac object PKS 2155-304 was observed by XMM for two essentially uninterrupted periods of ~11 and 16 hr on 30-31 May 2000. The strongest variations occurred in the...
ATP-dependent membrane assembly of F-actin facilitates membrane fusion (2001)
Jahraus,Andrea, Egeberg,Morten, Hinner,Bernhard, Habermann,Anja, Sackman,Erich, Pralle,Arnd, ...
We recently established an in vitro assay that monitors the fusion between latex-bead phagosomes and endocytic organelles in the presence of J774 macrophage cytosol (Jahraus et al., 1998). Here, we...
ATP-dependent membrane assembly of F-actin facilitates membrane fusion (2001)
Jahraus, Andrea, Egeberg, Morten, Hinner, Bernhard, Habermann, Anja, Sackman, Erich, Pralle, Arnd, ...
We recently established an in vitro assay that monitors the fusion between latex-bead phagosomes and endocytic organelles in the presence of J774 macrophage cytosol (Jahraus et al., 1998). Here, we...
Human cofilin forms oligomers exhibiting actin bundling activity (2001)
Pfannstiel, Jens, Cyrklaff, Marek, Habermann, Anja, Stoeva, Stanka, Griffiths, Gareth, Shoeman, Robert, ...
Human cofilin possesses the tendency for self-association, as indicated by the rapid formation of dimers and oligomers when reacted with water-soluble carbodiimide, Ellmans reagent, or glutathione...
Involvement of ezrin/moesin in de novo actin assembly on phagosomal membranes (2000)
Defacque,Hélène, Egeberg,Morten, Habermann,Anja, Diakonova,Maria, Roy,Christian, Mangeat,Paul, ...
The current study focuses on the molecular mechanisms responsible for actin assembly on a defined membrane surface: the phagosome. Mature phagosomes were surrounded by filamentous actin in vivo in...
Involvement of ezrin/moesin in de novo actin assembly on phagosomal membranes (2000)
Defacque, Hélène, Egeberg, Morten, Habermann, Anja, Diakonova, Maria, Roy, Christian, Mangeat, Paul, ...
The current study focuses on the molecular mechanisms responsible for actin assembly on a defined membrane surface: the phagosome. Mature phagosomes were surrounded by filamentous actin in vivo in...
Lipid hydroperoxide levels in plant tissues (2000)
Griffiths, Gareth, Leverentz, Michael, Silkowski, Helena, Gill, Narinder, Sánchez-Serrano, José J.
Hydroperoxides are the primary oxygenated products of polyunsaturated fatty acids and are key intermediates in the octadecanoid signalling pathway in plants. Lipid hydroperoxides (LHPO)...
Lysosomal Enzyme Trafficking between Phagosomes, Endosomes, and Lysosomes in J774 Macrophages (1998)
Claus,Volker, Jahraus,Andrea, Tjelle,Torunn, Berg,Trond, Kirschke,Heidrun, Faulstich,Heinz, ...
In this study we take advantage of recently developed methods using J774 macrophages to prepare enriched fractions of early endosomes, late endosomes, dense lysosomes, as well as phagosomes of...
Lysosomal Enzyme Trafficking between Phagosomes, Endosomes, and Lysosomes in J774 Macrophages (1998)
Claus, Volker, Jahraus, Andrea, Tjelle, Torunn, Berg, Trond, Kirschke, Heidrun, Faulstich, Heinz, ...
In this study we take advantage of recently developed methods using J774 macrophages to prepare enriched fractions of early endosomes, late endosomes, dense lysosomes, as well as phagosomes of...
A role for microtubule dynamics in phagosome movement (1998)
Ariel Blocker, Gareth Griffiths, Jean-christophe Olivo, Anthony A. Hyman, Fedor F. Severin
We have shown previously that intracellular phagosome movement requires microtubules. Here we provide evidence that within cells phagosomes display two different kinds of microtubule-based movements...
Fine structure immunocytochemistry / Gareth Griffiths (1993)
Griffiths, Gareth, Burke, Brion, Lucocq, John
Incluye bibliografía e índice
LINDBERG, SYLVIA, GRIFFITHS, GARETH
The effect of aluminium (Al) in vivo and in vitro on root plasma membranes has been studied in two sugar beet (Beta vulgaris L.) cultivars, Monohill (Al-sensitive) and Regina (relatively...
Zachgo, Sabine, Dobberstein, Bernhard, Griffiths, Gareth
Invariant chain (Ii) associated with MHC class II molecule is processed proteolytically via several distinct intermediates during its intracellular transport through endosomal compartments. Leupeptin...
Zachgo, Sabine, Dobberstein, Bernhard, Griffiths, Gareth
Invariant chain (Ii) associated with MHC class II molecule is processed proteolytically via several distinct intermediates during its intracellular transport through endosomal compartments. Leupeptin...
Kiebler, Michael, Pfaller, Rupert, Söllner, Thomas, Griffiths, Gareth, Horstmann, Heinz, Pfanner, Nikolaus, ...
A mitochondrial import receptor for the ADP/ATP carrier (1990)
Söllner, Thomas, Griffiths, Gareth, Pfanner, Nikolaus, Neupert, Walter
We have identified a mitochondrial outer membrane protein of 72 kd (MOM72) that exhibits the properties of an import receptor for the ADP/ATP carrier (AAC), the most abundant mitochondrial protein....
MOM19, an import receptor for mitochondrial precursor proteins (1989)
Söllner, Thomas, Griffiths, Gareth, Pfaller, Rupert, Pfanner, Nikolaus, Neupert, Walter
We have identified a 19 kd protein of the mitochondrial outer membrane (MOM19). Monospecific IgG and Fab fragments directed against MOM19 inhibit import of precursor proteins destined for the various...
A spectroscopic study of some cobaloximes. (1979)
Thesis (B.Sc.Hons.) (Chemistry)--University of Cape Town, 1979.
Locker, Jacomine Krijnse, Kuehn, Annett, Schleich, Sibylle, Rutter, Gaby, Hohenberg, Heinrich, Wepf, Roger, ...
The simpler of the two infectious forms of vaccinia virus, the intracellular mature virus (IMV) is known to infect cells less efficiently than the extracellular enveloped virus (EEV), which is...
GS32, a Novel Golgi SNARE of 32 kDa, Interacts Preferentially with Syntaxin 6
Wong, Siew Heng, Xu, Yue, Zhang, Tao, Griffiths, Gareth, Lowe, Stephen Loucian, Subramaniam, V. Nathan, ...
Syntaxin 1, synaptobrevins or vesicle-associated membrane proteins, and the synaptosome-associated protein of 25 kDa (SNAP-25) are key molecules involved in the docking and fusion of synaptic...
Wong, Siew Heng, Zhang, Tao, Xu, Yue, Subramaniam, V. Nathan, Griffiths, Gareth, Hong, Wanjin
Synaptobrevins/vesicle-associated membrane proteins (VAMPs) together with syntaxins and a synaptosome-associated protein of 25 kDa (SNAP-25) are the main components of a protein complex involved in...
ATP-dependent Membrane Assembly of F-Actin Facilitates Membrane Fusion
Jahraus, Andrea, Egeberg, Morten, Hinner, Bernhard, Habermann, Anja, Sackman, Erich, Pralle, Arnd, ...
We recently established an in vitro assay that monitors the fusion between latex-bead phagosomes and endocytic organelles in the presence of J774 macrophage cytosol (Jahraus et al., 1998). Here, we...
Myosin Va Bound to Phagosomes Binds to F-Actin and Delays Microtubule-dependent Motility
Al-Haddad, Ahmed, Shonn, Marion A., Redlich, Bärbel, Blocker, Ariel, Burkhardt, Janis K., Yu, Hanry, ...
We established a light microscopy-based assay that reconstitutes the binding of phagosomes purified from mouse macrophages to preassembled F-actin in vitro. Both endogenous myosin Va from mouse...
Molecular and Biochemical Characterization of Postharvest Senescence in Broccoli
Page, Tania, Griffiths, Gareth, Buchanan-Wollaston, Vicky
Postharvest senescence in broccoli (Brassica oleracea L. var Italica) florets results in phenotypic changes similar to those seen in developmental leaf senescence. To compare these two processes in...
Phosphoinositides Regulate Membrane-dependent Actin Assembly by Latex Bead Phagosomes
Defacque, Hélène, Bos, Evelyne, Garvalov, Boyan, Barret, Cécile, Roy, Christian, Mangeat, Paul, ...
Actin assembly on membrane surfaces is an elusive process in which several phosphoinositides (PIPs) have been implicated. We have reconstituted actin assembly using a defined membrane surface, the...
Lorenzo, María M., Galindo, Inmaculada, Griffiths, Gareth, Blasco, Rafael
The extracellular enveloped virus (EEV) form of vaccinia virus is bound by an envelope which is acquired by wrapping of intracellular virus particles with cytoplasmic vesicles containing trans-Golgi...
Characterization of the Coronavirus Mouse Hepatitis Virus Strain A59 Small Membrane Protein E
Raamsman, Martin J. B., Locker, Jacomine Krijnse, De Hooge, Alphons, Griffiths, Gareth, Vennema, Harry, ...
The small envelope (E) protein has recently been shown to play an essential role in the assembly of coronaviruses. Expression studies revealed that for formation of the viral envelope, actually only...
Pedersen, Ketil, Snijder, Eric J., Schleich, Sibylle, Roos, Norbert, Griffiths, Gareth, Locker, Jacomine Krijnse
The entry of vaccinia virus (VV) into the host cell results in the delivery of the double-stranded DNA genome-containing core into the cytoplasm. The core is disassembled, releasing the viral DNA in...
Structure and Assembly of Intracellular Mature Vaccinia Virus: Isolated-Particle Analysis
Griffiths, Gareth, Wepf, Roger, Wendt, Thomas, Locker, Jacomine Krijnse, Cyrklaff, Marek, Roos, Norbert
In a series of papers, we have provided evidence that during its assembly vaccinia virus is enveloped by a membrane cisterna that originates from a specialized, virally modified, smooth-membraned...
Structure and Assembly of Intracellular Mature Vaccinia Virus: Thin-Section Analyses
Griffiths, Gareth, Roos, Norbert, Schleich, Sybille, Locker, Jacomine Krijnse
In the preceding study (see accompanying paper), we showed by a variety of different techniques that intracellular mature vaccinia virus (vaccinia IMV) is unexpectedly complex in its structural...
Leverentz, Michael K., Wagstaff, Carol, Rogers, Hilary J., Stead, Anthony D., Chanasut, Usawadee, Silkowski, Helena, ...
The role of lipoxygenase (lox) in senescence of Alstroemeria peruviana flowers was investigated using a combination of in vitro assays and chemical profiling of the lipid oxidation products...
Involvement of ezrin/moesin in de novo actin assembly on phagosomal membranes
Defacque, Hélène, Egeberg, Morten, Habermann, Anja, Diakonova, Maria, Roy, Christian, Mangeat, Paul, ...
The current study focuses on the molecular mechanisms responsible for actin assembly on a defined membrane surface: the phagosome. Mature phagosomes were surrounded by filamentous actin in vivo in...
Kjeken, Rune, Egeberg, Morten, Habermann, Anja, Kuehnel, Mark, Peyron, Pascale, Floetenmeyer, Matthias, ...
Actin is implicated in membrane fusion, but the precise mechanisms remain unclear. We showed earlier that membrane organelles catalyze the de novo assembly of F-actin that then facilitates the fusion...
Disruption of Toxoplasma gondii Parasitophorous Vacuoles by the Mouse p47-Resistance GTPases
Martens, Sascha, Parvanova, Iana, Zerrahn, Jens, Griffiths, Gareth, Schell, Gudrun, Reichmann, Gaby, ...
The p47 GTPases are essential for interferon-γ-induced cell-autonomous immunity against the protozoan parasite, Toxoplasma gondii, in mice, but the mechanism of resistance is poorly understood. We...
Sancho, M. Carmen, Schleich, Sibylle, Griffiths, Gareth, Krijnse-Locker, Jacomine
It has previously been shown that upon infection of HeLa cells with modified vaccinia virus Ankara (MVA), assembly is blocked at a late stage of infection and immature virions (IVs) accumulate (G....
Prost, Isabelle, Dhondt, Sandrine, Rothe, Grit, Vicente, Jorge, Rodriguez, Maria José, Kift, Neil, ...
Plant oxylipins are a large family of metabolites derived from polyunsaturated fatty acids. The characterization of mutants or transgenic plants affected in the biosynthesis or perception of...
Locker, Jacomine Krijnse, Kuehn, Annett, Schleich, Sibylle, Rutter, Gaby, Hohenberg, Heinrich, Wepf, Roger, ...
The simpler of the two infectious forms of vaccinia virus, the intracellular mature virus (IMV) is known to infect cells less efficiently than the extracellular enveloped virus (EEV), which is...
GS32, a Novel Golgi SNARE of 32 kDa, Interacts Preferentially with Syntaxin 6
Wong, Siew Heng, Xu, Yue, Zhang, Tao, Griffiths, Gareth, Lowe, Stephen Loucian, Subramaniam, V. Nathan, ...
Syntaxin 1, synaptobrevins or vesicle-associated membrane proteins, and the synaptosome-associated protein of 25 kDa (SNAP-25) are key molecules involved in the docking and fusion of synaptic...
Wong, Siew Heng, Zhang, Tao, Xu, Yue, Subramaniam, V. Nathan, Griffiths, Gareth, Hong, Wanjin
Synaptobrevins/vesicle-associated membrane proteins (VAMPs) together with syntaxins and a synaptosome-associated protein of 25 kDa (SNAP-25) are the main components of a protein complex involved in...
ATP-dependent Membrane Assembly of F-Actin Facilitates Membrane Fusion
Jahraus, Andrea, Egeberg, Morten, Hinner, Bernhard, Habermann, Anja, Sackman, Erich, Pralle, Arnd, ...
We recently established an in vitro assay that monitors the fusion between latex-bead phagosomes and endocytic organelles in the presence of J774 macrophage cytosol (Jahraus et al., 1998). Here, we...
Myosin Va Bound to Phagosomes Binds to F-Actin and Delays Microtubule-dependent Motility
Al-Haddad, Ahmed, Shonn, Marion A., Redlich, Bärbel, Blocker, Ariel, Burkhardt, Janis K., Yu, Hanry, ...
We established a light microscopy-based assay that reconstitutes the binding of phagosomes purified from mouse macrophages to preassembled F-actin in vitro. Both endogenous myosin Va from mouse...
Molecular and Biochemical Characterization of Postharvest Senescence in Broccoli
Page, Tania, Griffiths, Gareth, Buchanan-Wollaston, Vicky
Postharvest senescence in broccoli (Brassica oleracea L. var Italica) florets results in phenotypic changes similar to those seen in developmental leaf senescence. To compare these two processes in...
Phosphoinositides Regulate Membrane-dependent Actin Assembly by Latex Bead Phagosomes
Defacque, Hélène, Bos, Evelyne, Garvalov, Boyan, Barret, Cécile, Roy, Christian, Mangeat, Paul, ...
Actin assembly on membrane surfaces is an elusive process in which several phosphoinositides (PIPs) have been implicated. We have reconstituted actin assembly using a defined membrane surface, the...
Lorenzo, María M., Galindo, Inmaculada, Griffiths, Gareth, Blasco, Rafael
The extracellular enveloped virus (EEV) form of vaccinia virus is bound by an envelope which is acquired by wrapping of intracellular virus particles with cytoplasmic vesicles containing trans-Golgi...
Characterization of the Coronavirus Mouse Hepatitis Virus Strain A59 Small Membrane Protein E
Raamsman, Martin J. B., Locker, Jacomine Krijnse, De Hooge, Alphons, Griffiths, Gareth, Vennema, Harry, ...
The small envelope (E) protein has recently been shown to play an essential role in the assembly of coronaviruses. Expression studies revealed that for formation of the viral envelope, actually only...
Pedersen, Ketil, Snijder, Eric J., Schleich, Sibylle, Roos, Norbert, Griffiths, Gareth, Locker, Jacomine Krijnse
The entry of vaccinia virus (VV) into the host cell results in the delivery of the double-stranded DNA genome-containing core into the cytoplasm. The core is disassembled, releasing the viral DNA in...
Structure and Assembly of Intracellular Mature Vaccinia Virus: Isolated-Particle Analysis
Griffiths, Gareth, Wepf, Roger, Wendt, Thomas, Locker, Jacomine Krijnse, Cyrklaff, Marek, Roos, Norbert
In a series of papers, we have provided evidence that during its assembly vaccinia virus is enveloped by a membrane cisterna that originates from a specialized, virally modified, smooth-membraned...
Structure and Assembly of Intracellular Mature Vaccinia Virus: Thin-Section Analyses
Griffiths, Gareth, Roos, Norbert, Schleich, Sybille, Locker, Jacomine Krijnse
In the preceding study (see accompanying paper), we showed by a variety of different techniques that intracellular mature vaccinia virus (vaccinia IMV) is unexpectedly complex in its structural...
Sancho, M. Carmen, Schleich, Sibylle, Griffiths, Gareth, Krijnse-Locker, Jacomine
It has previously been shown that upon infection of HeLa cells with modified vaccinia virus Ankara (MVA), assembly is blocked at a late stage of infection and immature virions (IVs) accumulate (G....
Leverentz, Michael K., Wagstaff, Carol, Rogers, Hilary J., Stead, Anthony D., Chanasut, Usawadee, Silkowski, Helena, ...
The role of lipoxygenase (lox) in senescence of Alstroemeria peruviana flowers was investigated using a combination of in vitro assays and chemical profiling of the lipid oxidation products...
Involvement of ezrin/moesin in de novo actin assembly on phagosomal membranes
Defacque, Hélène, Egeberg, Morten, Habermann, Anja, Diakonova, Maria, Roy, Christian, Mangeat, Paul, ...
The current study focuses on the molecular mechanisms responsible for actin assembly on a defined membrane surface: the phagosome. Mature phagosomes were surrounded by filamentous actin in vivo in...
Kjeken, Rune, Egeberg, Morten, Habermann, Anja, Kuehnel, Mark, Peyron, Pascale, Floetenmeyer, Matthias, ...
Actin is implicated in membrane fusion, but the precise mechanisms remain unclear. We showed earlier that membrane organelles catalyze the de novo assembly of F-actin that then facilitates the fusion...
Disruption of Toxoplasma gondii Parasitophorous Vacuoles by the Mouse p47-Resistance GTPases
Martens, Sascha, Parvanova, Iana, Zerrahn, Jens, Griffiths, Gareth, Schell, Gudrun, Reichmann, Gaby, ...
The p47 GTPases are essential for interferon-γ-induced cell-autonomous immunity against the protozoan parasite, Toxoplasma gondii, in mice, but the mechanism of resistance is poorly understood. We...
Prost, Isabelle, Dhondt, Sandrine, Rothe, Grit, Vicente, Jorge, Rodriguez, Maria José, Kift, Neil, ...
Plant oxylipins are a large family of metabolites derived from polyunsaturated fatty acids. The characterization of mutants or transgenic plants affected in the biosynthesis or perception of...
Whole Cell Cryo-Electron Tomography Reveals Distinct Disassembly Intermediates of Vaccinia Virus
Cyrklaff, Marek, Linaroudis, Alexandros, Boicu, Marius, Chlanda, Petr, Baumeister, Wolfgang, Griffiths, Gareth, ...
At each round of infection, viruses fall apart to release their genome for replication, and then reassemble into stable particles within the same host cell. For most viruses, the structural details...
Phagosome proteomes open the way to a better understanding of phagosome function
Griffiths, Gareth, Mayorga, Luis
Determination of the global protein composition of phagosomes is a first step to understanding how these organelles carry out their function.
Integrated network reconstruction, visualization and analysis using YANAsquare
Schwarz, Roland, Liang, Chunguang, Kaleta, Christoph, Kühnel, Mark, Hoffmann, Eik, Kuznetsov, Sergei, ...
A course with a difference. Fighting infectious diseases with technology and knowledge-transfer
Pulliainen, Arto Tapio, Enninga, Jost, Fernández-Arenas, Elena, Griffiths, Gareth
Filopodia act as phagocytic tentacles and pull with discrete steps and a load-dependent velocity
Kress, Holger, Stelzer, Ernst H. K., Holzer, Daniela, Buss, Folma, Griffiths, Gareth, Rohrbach, Alexander
Filopodia are thin, spike-like cell surface protrusions containing bundles of parallel actin filaments. So far, filopodial dynamics has mainly been studied in the context of cell motility on...
Dia1 and IQGAP1 interact in cell migration and phagocytic cup formation
Brandt, Dominique T., Marion, Sabrina, Griffiths, Gareth, Watanabe, Takashi, Kaibuchi, Kozo, Grosse, Robert
The Diaphanous-related formin Dia1 nucleates actin polymerization, thereby regulating cell shape and motility. Mechanisms that control the cellular location of Dia1 to spatially define actin...
An Unconventional Role for Cytoplasmic Disulfide Bonds in Vaccinia Virus Proteins
Locker, Jacomine Krijnse, Griffiths, Gareth
Previous data have shown that reducing agents disrupt the structure of vaccinia virus (vv). Here, we have analyzed the disulfide bonding of vv proteins in detail. In vv-infected cells cytoplasmically...
Scheel, Jochen, Pepperkok, Rainer, Lowe, Martin, Griffiths, Gareth, Kreis, Thomas E.
Addition of brefeldin A (BFA) to mammalian cells rapidly results in the removal of coatomer from membranes and subsequent delivery of Golgi enzymes to the endoplasmic reticulum (ER). Microinjected...
Molecular Requirements for Bi-directional Movement of Phagosomes Along Microtubules
Blocker, Ariel, Severin, Fedor F., Burkhardt, Janis K., Bingham, James B., Yu, Hanry, Olivo, Jean-Christophe, ...
Microtubules facilitate the maturation of phagosomes by favoring their interactions with endocytic compartments. Here, we show that phagosomes move within cells along tracks of several microns...
The conformational state of Tes regulates its zyxin-dependent recruitment to focal adhesions
Garvalov, Boyan K., Higgins, Theresa E., Sutherland, James D., Zettl, Markus, Scaplehorn, Niki, Köcher, Thomas, ...
The function of the human Tes protein, which has extensive similarity to zyxin in both sequence and domain organization, is currently unknown. We now show that Tes is a component of focal adhesions...
Zuber, Benoît, Chami, Mohamed, Houssin, Christine, Dubochet, Jacques, Griffiths, Gareth, Daffé, Mamadou
The cell envelope of mycobacteria, which include the causative agents of tuberculosis and leprosy, is crucial for their success as pathogens. Despite a continued strong emphasis on identifying the...
Modelling phagosomal lipid networks that regulate actin assembly
Kühnel, Mark, Mayorga, Luis S, Dandekar, Thomas, Thakar, Juilee, Schwarz, Roland, Anes, Elsa, ...
Axe, Elizabeth L., Walker, Simon A., Manifava, Maria, Chandra, Priya, Roderick, H. Llewelyn, Habermann, Anja, ...
Autophagy is the engulfment of cytosol and organelles by double-membrane vesicles termed autophagosomes. Autophagosome formation is known to require phosphatidylinositol 3-phosphate (PI(3)P) and...