Gary E. Ward

Identification of the Moving Junction Complex of Toxoplasma gondii: A Collaboration between Distinct Secretory Organelles (2005)

David L. Alexander, Jeffrey Mital, Gary E. Ward, Peter Bradley, John C. Boothroyd

Apicomplexan parasites, including Toxoplasma gondii and Plasmodium sp., are obligate intracellular protozoa. They enter into a host cell by attaching to and then creating an invagination in the host...

Chemotaxis of Arbacia punctulata spermatozoa to resact, a peptide from the egg jelly layer (1985)

Ward, Gary E., Brokaw, Charles J., Garbers, David L., Vacquier, Victor D.

Resact, a peptide of known sequence isolated from the jelly layer of Arbacia punctulata eggs, is a potent chemoattractant for A. punctulata spermatozoa. The chemotactic response is concentration...

Clostridium septicum Alpha-Toxin Is Active against the Parasitic Protozoan Toxoplasma gondii and Targets Members of the SAG Family of Glycosylphosphatidylinositol-Anchored Surface Proteins

Wichroski, Michael J., Melton, Jody A., Donahue, Carolyn G., Tweten, Rodney K., Ward, Gary E.

As is the case with many other protozoan parasites, glycosylphosphatidylinositol (GPI)-anchored proteins dominate the surface of Toxoplasma gondii tachyzoites. The mechanisms by which T. gondii...

Biosynthesis of Glycosylphosphatidylinositol Is Essential to the Survival of the Protozoan Parasite Toxoplasma gondii

Wichroski, Michael J., Ward, Gary E.

The PIGA gene from Toxoplasma gondii has been cloned and characterized. Like mammalian PIGA, the transmembrane and C-terminal domains are sufficient to direct localization to the parasite endoplasmic...

Dephosphorylation of a major sperm membrane protein is induced by egg jelly during sea urchin fertilization

Ward, Gary E., Vacquier, Victor D.

A 160-kilodalton (kDa) protein of Arbacia punctulata sperm is constitutively phosphorylated on serine residues, as shown by in vivo32PO43- labeling. Exposure of sperm to solubilized egg jelly results...

A small-molecule approach to studying invasive mechanisms of Toxoplasma gondii

Carey, Kimberly L., Westwood, Nicholas J., Mitchison, Timothy J., Ward, Gary E.

Toxoplasma gondii is the most common protozoan parasite of humans. Infection with T. gondii can lead to life-threatening disease as a result of repeated cycles of host cell invasion, parasite...

The Toxoplasma gondii Rhoptry Protein ROP4 Is Secreted into the Parasitophorous Vacuole and Becomes Phosphorylated in Infected Cells

Carey, Kimberly L., Jongco, Artemio M., Kim, Kami, Ward, Gary E.

Many intracellular pathogens are separated from the cytosol of their host cells by a vacuole membrane. This membrane serves as a critical interface between the pathogen and the host cell, across...

Conditional Expression of Toxoplasma gondii Apical Membrane Antigen-1 (TgAMA1) Demonstrates That TgAMA1 Plays a Critical Role in Host Cell Invasion

Mital, Jeffrey, Meissner, Markus, Soldati, Dominique, Ward, Gary E.

Toxoplasma gondii is an obligate intracellular parasite and an important human pathogen. Relatively little is known about the proteins that orchestrate host cell invasion by T. gondii or related...

Identification of the Moving Junction Complex of Toxoplasma gondii: A Collaboration between Distinct Secretory Organelles

Alexander, David L, Mital, Jeffrey, Ward, Gary E, Bradley, Peter, Boothroyd, John C

Apicomplexan parasites, including Toxoplasma gondii and Plasmodium sp., are obligate intracellular protozoa. They enter into a host cell by attaching to and then creating an invagination in the host...

Clostridium septicum Alpha-Toxin Is Active against the Parasitic Protozoan Toxoplasma gondii and Targets Members of the SAG Family of Glycosylphosphatidylinositol-Anchored Surface Proteins

Wichroski, Michael J., Melton, Jody A., Donahue, Carolyn G., Tweten, Rodney K., Ward, Gary E.

As is the case with many other protozoan parasites, glycosylphosphatidylinositol (GPI)-anchored proteins dominate the surface of Toxoplasma gondii tachyzoites. The mechanisms by which T. gondii...

Biosynthesis of Glycosylphosphatidylinositol Is Essential to the Survival of the Protozoan Parasite Toxoplasma gondii

Wichroski, Michael J., Ward, Gary E.

The PIGA gene from Toxoplasma gondii has been cloned and characterized. Like mammalian PIGA, the transmembrane and C-terminal domains are sufficient to direct localization to the parasite endoplasmic...

Dephosphorylation of a major sperm membrane protein is induced by egg jelly during sea urchin fertilization

Ward, Gary E., Vacquier, Victor D.

A 160-kilodalton (kDa) protein of Arbacia punctulata sperm is constitutively phosphorylated on serine residues, as shown by in vivo32PO43- labeling. Exposure of sperm to solubilized egg jelly results...

A small-molecule approach to studying invasive mechanisms of Toxoplasma gondii

Carey, Kimberly L., Westwood, Nicholas J., Mitchison, Timothy J., Ward, Gary E.

Toxoplasma gondii is the most common protozoan parasite of humans. Infection with T. gondii can lead to life-threatening disease as a result of repeated cycles of host cell invasion, parasite...

The Toxoplasma gondii Rhoptry Protein ROP4 Is Secreted into the Parasitophorous Vacuole and Becomes Phosphorylated in Infected Cells

Carey, Kimberly L., Jongco, Artemio M., Kim, Kami, Ward, Gary E.

Many intracellular pathogens are separated from the cytosol of their host cells by a vacuole membrane. This membrane serves as a critical interface between the pathogen and the host cell, across...

Conditional Expression of Toxoplasma gondii Apical Membrane Antigen-1 (TgAMA1) Demonstrates That TgAMA1 Plays a Critical Role in Host Cell Invasion

Mital, Jeffrey, Meissner, Markus, Soldati, Dominique, Ward, Gary E.

Toxoplasma gondii is an obligate intracellular parasite and an important human pathogen. Relatively little is known about the proteins that orchestrate host cell invasion by T. gondii or related...

Identification of the Moving Junction Complex of Toxoplasma gondii: A Collaboration between Distinct Secretory Organelles

Alexander, David L, Mital, Jeffrey, Ward, Gary E, Bradley, Peter, Boothroyd, John C

Apicomplexan parasites, including Toxoplasma gondii and Plasmodium sp., are obligate intracellular protozoa. They enter into a host cell by attaching to and then creating an invagination in the host...

Identification of PhIL1, a Novel Cytoskeletal Protein of the Toxoplasma gondii Pellicle, through Photosensitized Labeling with 5-[125I]Iodonaphthalene-1-Azide

Gilk, Stacey D., Raviv, Yossef, Hu, Ke, Murray, John M., Beckers, Con J. M., Ward, Gary E.

The pellicle of the protozoan parasite Toxoplasma gondii is a unique triple bilayer structure, consisting of the plasma membrane and two tightly apposed membranes of the underlying inner membrane...

Targeted Deletion of MIC5 Enhances Trimming Proteolysis of Toxoplasma Invasion Proteins▿

Brydges, Susannah D., Zhou, Xing Wang, Huynh, My-Hang, Harper, Jill M., Mital, Jeffrey, Adjogble, Koku D. Z., ...

Limited proteolysis of proteins transiently expressed on the surface of the opportunistic pathogen Toxoplasma gondii accompanies cell invasion and facilitates parasite migration across cell barriers...

GAP45 Phosphorylation Controls Assembly of the Toxoplasma Myosin XIV Complex▿

Gilk, Stacey D., Gaskins, Elizabeth, Ward, Gary E., Beckers, Con J. M.

Toxoplasma gondii motility is powered by the myosin XIV motor complex, which consists of the myosin XIV heavy chain (MyoA), the myosin light chain (MLC1), GAP45, and GAP50, the membrane anchor of the...