Sharples, Gary J., Whitby, Matthew C., Ryder, Lizanne, Lloyd, Robert G.
The RecG protein of Escherichia coli catalyses branch migration of Holliday junctions made by RecA and dissociates synthetic X junctions into duplex products in reactions that require hydrolysis of...
An E.coli RuvC mutant defective in cleavage of synthetic Holliday junctions (1993)
Sharples, Gary J., Lloyd, Robert G.
Escherichia coli RuvC protein is a specific endonuclease that resolves recombination intermediates into viable products. The structural features needed for RuvC activity were investigated by...
Processing of recombination intermediates by the RecG and RuvAB proteins of Escherichia coli (1993)
Lloyd, Robert G., Sharples, Gary J.
The RuvAB, RuvC and RecG proteins of Escherlchla coil process intermediates In recombination and DNA repair Into mature products. RuvAB and RecG catalyse branch migration of Holliday junctions, while...
A novel repeated DNA sequence located in the intergenic regions of bacterial chromosomes (1990)
Sharples, Gary J., Lloyd, Robert G.
We report the discovery of a novel group of highly conserved DNA sequences located within the intergenic regions of the chromosomes of Escherichia coll, Salmonella typhimurium and other bacteria....
Benson, Fiona E., Illing, Graham T., Sharples, Gary J., Lloyd, Robert G.
The nucieotlde sequence of a 2505 bp region of the Escherlrhia coli chromosome containing the LexA regulated ruv gene has been determined. A sequence of 1631 bp encoding two non-overlapping open...
λ Rap protein is a structure-specific endonuclease involved in phage recombination
Sharples, Gary J., Corbett, Lisa M., Graham, Ian R.
Bacteriophage λ encodes a number of genes involved in the recombinational repair of DNA double-strand breaks. The product of one of these genes, rap, has been purified. Truncated Rap proteins that...
A model for dsDNA translocation revealed by a structural motif common to RecG and Mfd proteins
Mahdi, Akeel A., Briggs, Geoffrey S., Sharples, Gary J., Wen, Qin, Lloyd, Robert G.
RecG protein differs from other helicases analysed to atomic resolution in that it mediates strand separation via translocation on double-stranded (ds) rather than single-stranded (ss) DNA. We...
Moore, Timothy, McGlynn, Peter, Ngo, Hien-Ping, Sharples, Gary J., Lloyd, Robert G.
PriA protein provides a means to load the DnaB replicative helicase at DNA replication fork and D loop structures, and is therefore a key factor in the rescue of stalled or broken forks and...
Loughlin, Michael F., Barnard, Faye M., Jenkins, David, Sharples, Gary J., Jenks, Peter J.
Homologous recombination contributes to the extraordinary genetic diversity of Helicobacter pylori and may be critical for surface antigen expression and adaptation to environmental challenges within...
The acidic pin of RuvA modulates Holliday junction binding and processing by the RuvABC resolvasome
Ingleston, Stuart M., Sharples, Gary J., Lloyd, Robert G.
Holliday junctions are four-way branched DNA structures formed during recombination, replication and repair. They are processed in Escherichia coli by the RuvA, RuvB and RuvC proteins. RuvA targets...
DNA Binding by the Meningococcal RdgC Protein, Associated with Pilin Antigenic Variation
Moore, Timothy, Sharples, Gary J., Lloyd, Robert G.
The RdgC protein of Neisseria gonorrhoeae is required for efficient pilin antigenic variation, although its precise role has yet to be established. We demonstrate that the nearly identical RdgC from...
Maxwell, Karen L., Reed, Patricia, Zhang, Rong-guang, Beasley, Steven, Walmsley, Adrian R., Curtis, Fiona A., ...
Genetic recombination in bacteriophage λ relies on DNA end processing by Exo to expose 3′-tailed strands for annealing and exchange by β protein. Phage λ encodes an additional recombinase, Orf,...
Sanchez, Humberto, Kidane, Dawit, Reed, Patricia, Curtis, Fiona A., Cozar, M. Castillo, Graumann, Peter L., ...
In models of Escherichia coli recombination and DNA repair, the RuvABC complex directs the branch migration and resolution of Holliday junction DNA. To probe the validity of the E. coli paradigm, we...
λ Rap protein is a structure-specific endonuclease involved in phage recombination
Sharples, Gary J., Corbett, Lisa M., Graham, Ian R.
Bacteriophage λ encodes a number of genes involved in the recombinational repair of DNA double-strand breaks. The product of one of these genes, rap, has been purified. Truncated Rap proteins that...
A model for dsDNA translocation revealed by a structural motif common to RecG and Mfd proteins
Mahdi, Akeel A., Briggs, Geoffrey S., Sharples, Gary J., Wen, Qin, Lloyd, Robert G.
RecG protein differs from other helicases analysed to atomic resolution in that it mediates strand separation via translocation on double-stranded (ds) rather than single-stranded (ss) DNA. We...
Moore, Timothy, McGlynn, Peter, Ngo, Hien-Ping, Sharples, Gary J., Lloyd, Robert G.
PriA protein provides a means to load the DnaB replicative helicase at DNA replication fork and D loop structures, and is therefore a key factor in the rescue of stalled or broken forks and...
Loughlin, Michael F., Barnard, Faye M., Jenkins, David, Sharples, Gary J., Jenks, Peter J.
Homologous recombination contributes to the extraordinary genetic diversity of Helicobacter pylori and may be critical for surface antigen expression and adaptation to environmental challenges within...
The acidic pin of RuvA modulates Holliday junction binding and processing by the RuvABC resolvasome
Ingleston, Stuart M., Sharples, Gary J., Lloyd, Robert G.
Holliday junctions are four-way branched DNA structures formed during recombination, replication and repair. They are processed in Escherichia coli by the RuvA, RuvB and RuvC proteins. RuvA targets...
DNA Binding by the Meningococcal RdgC Protein, Associated with Pilin Antigenic Variation
Moore, Timothy, Sharples, Gary J., Lloyd, Robert G.
The RdgC protein of Neisseria gonorrhoeae is required for efficient pilin antigenic variation, although its precise role has yet to be established. We demonstrate that the nearly identical RdgC from...
Maxwell, Karen L., Reed, Patricia, Zhang, Rong-guang, Beasley, Steven, Walmsley, Adrian R., Curtis, Fiona A., ...
Genetic recombination in bacteriophage λ relies on DNA end processing by Exo to expose 3′-tailed strands for annealing and exchange by β protein. Phage λ encodes an additional recombinase, Orf,...
Sanchez, Humberto, Kidane, Dawit, Reed, Patricia, Curtis, Fiona A., Cozar, M. Castillo, Graumann, Peter L., ...
In models of Escherichia coli recombination and DNA repair, the RuvABC complex directs the branch migration and resolution of Holliday junction DNA. To probe the validity of the E. coli paradigm, we...