George H. Jones

Molecular cloning and in vitro expression of a silent phenoxazinone synthase gene from Streptomyces lividans (1989)

Madu, Anthony C., Jones, George H.

Phenoxazinone synthase (PHS) catalyzes a step in actinomycin D biosynthesis in Streptomyces antibioticus. Two sequences from Streptomyces lividans that hybridize to the phs gene of S. antibioticus...

Function of ribosomes and glutamyl-tRNA isoacceptors in protein synthesis in regenerating skeletal muscle (1985)

Jones, George H.

Ribosomes from 8-day-regenerating rat skeletal muscle have been shown to be more active in poly(U)-directed polyphenylalanine synthesis than ribosomes from control muscle. This difference persists...

Time course of changes in protein synthesis in marcaine-induced skeletal muscle regeneration (1984)

Jones, George H.

The time course of the regeneration of rat skeletal muscle has been examined after injection of the myotoxic drug, Marcaine, to induce regeneration. Muscle wet weight decreases during the initial...

Acceptor activity, isoacceptor profiles and function in protein synthesis of transfer RNAs from regenerating skeletal muscle (1983)

Jones, George H.

Transfer RNAs have been prepared from control and regenerating rat skeletal muscle. The yield of tRNA is highest during the early stages of the regeneration process (5 and 8 days following the...

Protein synthesis in bupivacaine (Marcaine)-treated, regenerating skeletal muscle (1982)

Jones, George H.

Skeletal muscle regeneration has been induced by injection of the myotoxic drug bupivacaine (Marcaine) into the rat tibialis anterior muscle. Doses of 1.5 and 1.0% wt/vol produce significant levels...

Phenoxazinone synthase from Streptomyces antibioticus: Purification of the large and small enzyme forms (1981)

Choy, Henry A., Jones, George H.

Phenoxazinone synthase has been purified approximately 80-fold from 48-h-old cells of Streptomyces antibioticus. The purification procedure involves streptomycin sulfate and ammonium sulfate...

Purification of RNA polymerase from actinomycin producing and nonproducing cells of Streptomyces antibioticus (1979)

Jones, George H.

DNA-dependent RNA polymerase has been purified approximately 700-fold from 12-h-old cells of Streptomyces antibioticus and 400-fold from 48-h cells. Both enzymes appear nearly homogeneous as judged...

Protein synthesis in skeletal muscle following acute exhaustive exercise (1979)

Rogers, Peter A., Jones, George H., Faulkner, John A.

Cell-free and whole-tissue protein synthesis was studied in skeletal muscle of untrained male guinea pigs that had undergone a treadmill run to exhaustion. Experiments using explants from the...

Sensitivity of transcription by purified RNA polymerase to actinomycin (1978)

Jones, George H.

DNA-dependent RNA polymerase has been purified 400-fold from 48 hr old, actinomycin producing cells of . Sodium dodecyl sulfate polyacrylamide gel electrophoresis of the purified polymerase reveals...

Effect of marcaine, a myotoxic drug, on macromolecular synthesis in muscle (1978)

Johnson, Marcia E., Jones, George H.

The effects of Marcaine (bupivacaine) on RNA and protein synthesis in skeletal muscle have been studied. The drug did not affect RNA synthesis by pieces of rat tibialis anterior at concentrations as...

Stimulation of protein synthesis "in vitro" by transcripts of mouse myeloma chromatin (1977)

Jones, George H.

SummaryWheat germ RNA polymerase II has been used to synthesize RNA's from mouse myeloma chromatin. The newly synthesized RNA has been shown to stimulate amino acid incorporation in a cell-free...

On the efficacy of commonly used ribonuclease inhibitors (1976)

Jones, George H.

The ability of a number of commonly used inhibitors to inhibit pancreatic ribonuclease has been studied. At ribonuclease concentrations of 10 or 100 g/ml, heparin, polyvinylsulfate and proteinase K,...

Ribonucleic acid synthesis in streptomyces antibioticus: Stable ribonucleic acid species synthesized by young and old cells (1975)

Jones, George H.

In studies of RNA synthesis by intact cells and cell-free extracts of , it has been found that 48 hr cells (producing actinomycin) and cell-free extracts are less efficient than 12 hr cells (not...

Studies on the initiation of protein synthesis in mouse myeloma tumors (1975)

Jones, George H.

35S- and 3H-labeled short, nascent peptides have been extracted from mouse myeloma ribosomes after incubation of myeloma fragments with [35S]methionine and [3H]amino acids. Edman analysis of these...

Morphological changes accompanying actinomycin production in Streptomyces antibioticus (1974)

Collett, Marc S., Jones, George H.

The fine structure of the actinomycin producing bacterium Streptomyces antibioticus has been studied. The typical appearance of freshly inoculated cells is one of a generally electron dense...

A protein related to immunoglobulin light chain detected in mouse myeloma cells (1973)

Jones, George H.

A protein (denoted L') which is similar in structure to immunoglobulin light chain has been isolated from the mouse plasma cell tumor, RPC-20. L' has a molecular weight which is about 6000 daltons...

Actinomycin Production Persists in a Strain of Streptomyces antibioticus Lacking Phenoxazinone Synthase

Jones, George H.

Truncated fragments of the phenoxazinone synthase gene, phsA, were prepared by the PCR. The resulting fragments were cloned into conjugative plasmid pKC1132 and transferred to Streptomyces...

relA Is Required for Actinomycin Production in Streptomyces antibioticus

Hoyt, Shannan, Jones, George H.

The relA gene from Streptomyces antibioticus has been cloned and sequenced. The gene encodes a protein with an Mr of 93,653, which is 91% identical to the corresponding protein from Streptomyces...

Organization and Expression of the Polynucleotide Phosphorylase Gene (pnp) of Streptomyces: Processing of pnp Transcripts in Streptomyces antibioticus

Bralley, Patricia, Jones, George H.

We have examined the expression of pnp encoding the 3′-5′-exoribonuclease, polynucleotide phosphorylase, in Streptomyces antibioticus. We show that the rpsO-pnp operon is transcribed from at...

Addition of Poly(A) and Heteropolymeric 3′ Ends in Bacillus subtilis Wild-Type and Polynucleotide Phosphorylase-Deficient Strains

Campos-Guillén, Juan, Bralley, Patricia, Jones, George H., Bechhofer, David H., Olmedo-Alvarez, Gabriela

Polyadenylation plays a role in decay of some bacterial mRNAs, as well as in the quality control of stable RNA. In Escherichia coli, poly(A) polymerase I (PAP I) is the main polyadenylating enzyme,...

A Phylogeny of Bacterial RNA Nucleotidyltransferases: Bacillus halodurans Contains Two tRNA Nucleotidyltransferases

Bralley, Patricia, Chang, Samantha A., Jones, George H.

We have analyzed the distribution of RNA nucleotidyltransferases from the family that includes poly(A) polymerases (PAP) and tRNA nucleotidyltransferases (TNT) in 43 bacterial species. Genes of...

Actinomycin Production Persists in a Strain of Streptomyces antibioticus Lacking Phenoxazinone Synthase

Jones, George H.

Truncated fragments of the phenoxazinone synthase gene, phsA, were prepared by the PCR. The resulting fragments were cloned into conjugative plasmid pKC1132 and transferred to Streptomyces...

relA Is Required for Actinomycin Production in Streptomyces antibioticus

Hoyt, Shannan, Jones, George H.

The relA gene from Streptomyces antibioticus has been cloned and sequenced. The gene encodes a protein with an Mr of 93,653, which is 91% identical to the corresponding protein from Streptomyces...

Organization and Expression of the Polynucleotide Phosphorylase Gene (pnp) of Streptomyces: Processing of pnp Transcripts in Streptomyces antibioticus

Bralley, Patricia, Jones, George H.

We have examined the expression of pnp encoding the 3′-5′-exoribonuclease, polynucleotide phosphorylase, in Streptomyces antibioticus. We show that the rpsO-pnp operon is transcribed from at...

Addition of Poly(A) and Heteropolymeric 3′ Ends in Bacillus subtilis Wild-Type and Polynucleotide Phosphorylase-Deficient Strains

Campos-Guillén, Juan, Bralley, Patricia, Jones, George H., Bechhofer, David H., Olmedo-Alvarez, Gabriela

Polyadenylation plays a role in decay of some bacterial mRNAs, as well as in the quality control of stable RNA. In Escherichia coli, poly(A) polymerase I (PAP I) is the main polyadenylating enzyme,...

A Phylogeny of Bacterial RNA Nucleotidyltransferases: Bacillus halodurans Contains Two tRNA Nucleotidyltransferases

Bralley, Patricia, Chang, Samantha A., Jones, George H.

We have analyzed the distribution of RNA nucleotidyltransferases from the family that includes poly(A) polymerases (PAP) and tRNA nucleotidyltransferases (TNT) in 43 bacterial species. Genes of...

Kinetics of Polynucleotide Phosphorylase: Comparison of Enzymes from Streptomyces and Escherichia coli and Effects of Nucleoside Diphosphates▿

Chang, Samantha A., Cozad, Madeline, Mackie, George A., Jones, George H.

We examined the activity of polynucleotide phosphorylase (PNPase) from Streptomyces coelicolor, Streptomyces antibioticus, and Escherichia coli in phosphorolysis using substrates derived from the...

Geobacter sulfurreducens Contains Separate C- and A-Adding tRNA Nucleotidyltransferases and a Poly(A) Polymerase▿ †

Bralley, Patricia, Cozad, Madeline, Jones, George H.

The genome of Geobacter sulfurreducens contains three genes whose sequences are quite similar to sequences encoding known members of an RNA nucleotidyltransferase superfamily that includes tRNA...