Exploiting Chemical Libraries, Structure, and Genomics in the Search for Kinase Inhibitors (1998)
Kim, Sung-Hou, Meijer, Laurent, LeClerc, Sophie, Barnes, Georjana, Morgan, David O., Espinoza, F. Hernan, ...
Selective protein kinase inhibitors were developed on the basis of the unexpected binding mode of 2,6,9-trisubstituted purines to the adenosine triphosphate-binding site of the human cyclin-dependent...
Exploiting Chemical Libraries, Structure, and Genomics in the Search for Kinase Inhibitors (1998)
Lockhart, David J., Kim, Sung-Hou, Meijer, Laurent, LeClerc, Sophie, Barnes, Georjana, Morgan, David O., ...
Selective protein kinase inhibitors were developed on the basis of the unexpected binding mode of 2,6,9-trisubstituted purines to the adenosine triphosphate-binding site of the human cyclin-dependent...
Thesis (Ph. D. in Biochemistry)--University of California, Berkeley, Dec. 1986.
In mammalian cells, the Ku heterodimer is involved in DNA double-strand-break recognition and repair. We have established in yeast a connection between Ku activity and DNA double-strand-break damage...
Enquist-Newman, Maria, Cheeseman, Iain M., Van Goor, David, Drubin, David G., Meluh, Pamela B., Barnes, Georjana
We showed recently that a complex between Duo1p and Dam1p is required for both spindle integrity and kinetochore function in the budding yeast Saccharomyces cerevisiae. To extend our understanding of...
Kinetochore Protein Interactions and their Regulation by the Aurora Kinase Ipl1pD⃞
Shang, Ching, Hazbun, Tony R., Cheeseman, Iain M., Aranda, Jennifer, Fields, Stanley, Drubin, David G., ...
Although there has been a recent explosion in the identification of budding yeast kinetochore components, the physical interactions that underlie kinetochore function remain obscure. To better...
In mammalian cells, the Ku heterodimer is involved in DNA double-strand-break recognition and repair. We have established in yeast a connection between Ku activity and DNA double-strand-break damage...
Enquist-Newman, Maria, Cheeseman, Iain M., Van Goor, David, Drubin, David G., Meluh, Pamela B., Barnes, Georjana
We showed recently that a complex between Duo1p and Dam1p is required for both spindle integrity and kinetochore function in the budding yeast Saccharomyces cerevisiae. To extend our understanding of...
Kinetochore Protein Interactions and their Regulation by the Aurora Kinase Ipl1pD⃞
Shang, Ching, Hazbun, Tony R., Cheeseman, Iain M., Aranda, Jennifer, Fields, Stanley, Drubin, David G., ...
Although there has been a recent explosion in the identification of budding yeast kinetochore components, the physical interactions that underlie kinetochore function remain obscure. To better...
A Protein Interaction Map of the Mitotic Spindle
Wong, Jonathan, Nakajima, Yuko, Westermann, Stefan, Shang, Ching, Kang, Jung-seog, Goodner, Crystal, ...
The mitotic spindle consists of a complex network of proteins that segregates chromosomes in eukaryotes. To strengthen our understanding of the molecular composition, organization, and regulation of...
Saccharomyces cerevisiae Duo1p and Dam1p, Novel Proteins Involved in Mitotic Spindle Function
Hofmann, Christian, Cheeseman, Iain M., Goode, Bruce L., McDonald, Kent L., Barnes, Georjana, Drubin, David G.
In this paper, we describe the identification and characterization of two novel and essential mitotic spindle proteins, Duo1p and Dam1p. Duo1p was isolated because its overexpression caused defects...
Goode, Bruce L., Wong, Jonathan J., Butty, Anne-Christine, Peter, Matthias, McCormack, Ashley L., Yates, John R., ...
Coronin is a highly conserved actin-associated protein that until now has had unknown biochemical activities. Using microtubule affinity chromatography, we coisolated actin and a homologue of...
Kang, Jung-seog, Cheeseman, Iain M., Kallstrom, George, Velmurugan, Soundarapandian, Barnes, Georjana, Chan, Clarence S.M.
We have shown previously that Ipl1 and Sli15 are required for chromosome segregation in Saccharomyces cerevisiae. Sli15 associates directly with the Ipl1 protein kinase and these two proteins...
Architecture of the budding yeast kinetochore reveals a conserved molecular core
Westermann, Stefan, Cheeseman, Iain M., Anderson, Scott, Yates, John R., Drubin, David G., Barnes, Georjana
How kinetochore proteins are organized to connect chromosomes to spindle microtubules, and whether any structural and organizational themes are common to kinetochores from distantly related...
Direct regulation of Arp2/3 complex activity and function by the actin binding protein coronin
Humphries, Christine L., Balcer, Heath I., D'Agostino, Jessica L., Winsor, Barbara, Drubin, David G., Barnes, Georjana, ...
Mechanisms for activating the actin-related protein 2/3 (Arp2/3) complex have been the focus of many recent studies. Here, we identify a novel mode of Arp2/3 complex regulation mediated by the highly...
Activation of the Arp2/3 Complex by the Actin Filament Binding Protein Abp1p
Goode, Bruce L., Rodal, Avital A., Barnes, Georjana, Drubin, David G.
The actin-related protein (Arp) 2/3 complex plays a central role in assembly of actin networks. Because distinct actin-based structures mediate diverse processes, many proteins are likely to make...
Mitotic Spindle Integrity and Kinetochore Function Linked by the Duo1p/Dam1p Complex
Cheeseman, Iain M., Enquist-Newman, Maria, Müller-Reichert, Thomas, Drubin, David G., Barnes, Georjana
Duo1p and Dam1p were previously identified as spindle proteins in the budding yeast, Saccharomyces cerevisiae. Here, analyses of a diverse collection of duo1 and dam1 alleles were used to develop a...
Cheeseman, Iain M., Drubin, David G., Barnes, Georjana
Although the budding yeast centromere is extremely short (125 bp) compared to those of other eukaryotes, the kinetochore that assembles on this DNA displays a rich molecular complexity. Here, we...
Implication of a novel multiprotein Dam1p complex in outer kinetochore function
Cheeseman, Iain M., Brew, Christine, Wolyniak, Michael, Desai, Arshad, Anderson, Scott, Muster, Nemone, ...
Dam1p, Duo1p, and Dad1p can associate with each other physically and are required for both spindle integrity and kinetochore function in budding yeast. Here, we present our purification from yeast...
Grishchuk, Ekaterina L., Efremov, Artem K., Volkov, Vladimir A., Spiridonov, Ilia S., Gudimchuk, Nikita, Westermann, Stefan, ...
Accurate chromosome segregation during mitotic division of budding yeast depends on the multiprotein kinetochore complex, Dam1 (also known as DASH). Purified Dam1 heterodecamers encircle microtubules...
Nakajima, Yuko, Tyers, Randall G., Wong, Catherine C.L., Yates, John R., Drubin, David G., Barnes, Georjana
The Aurora kinase complex, also called the chromosomal passenger complex (CPC), is essential for faithful chromosome segregation and completion of cell division. In Fungi and Animalia, this complex...
Woodruff, Jeffrey B., Drubin, David G., Barnes, Georjana
Dynein is a minus-end–directed microtubule motor important for mitotic spindle positioning. In budding yeast, dynein activity is restricted to anaphase when the nucleus enters the bud neck, yet the...