FLO1 is a variable green beard gene that drives biofilm-like cooperation in budding yeast. (2008)
Smukalla, Scott, Caldara, Marina, Pochet, Nathalie, Beauvais, Anne, Guadagnini, Stephanie, Yan, Chen, ...
The budding yeast, Saccharomyces cerevisiae, has emerged as an archetype of eukaryotic cell biology. Here we show that S. cerevisiae is also a model for the evolution of cooperative behavior by...
Coding tandem repeats generate genetic diversity in Aspergillus fumigatus genes (2007)
Romano, Jacob, Shadkchan, Yona, Sharon, Haim, Fink, Gerald R, ...
Genes containing multiple coding mini- and microsattelite repeats are highly dynamic components of genomes. Frequent recombination events within these tandem repeats lead to changes in repeat...
Zumbuehl, Andreas, Ferreira, Lino, Kuhn, Duncan, Astashkina, Anna, Long, Lisa, Yeo, Yoon, ...
Fungi are increasingly identified as major pathogens in bloodstream infections, often involving indwelling devices. Materials with antifungal properties may provide an important deterrent to these...
Genes with internal repeats require the THO complex for transcription (2006)
Jansen, An, Runner, Vanessa M, Buratowski, Stephen, Fink, Gerald R
The evolutionarily conserved multisubunit THO complex, which is recruited to actively transcribed genes, is required for the efficient expression of FLO11 and other yeast genes that have long...
A Drug-Sensitive Genetic Network Masks Fungi from the Immune System (2006)
Robert T. Wheeler, Gerald R. Fink
Fungal pathogens can be recognized by the immune system via their β-glucan, a potent proinflammatory molecule that is present at high levels but is predominantly buried beneath a mannoprotein coat...
Disentangling information flow in the Ras-cAMP signaling network (2006)
Carter, Gregory W., Rupp, Steffen, Fink, Gerald R., Galitski, Timothy
The perturbation of signal-transduction molecules elicits genomic-expression effects that are typically neither restricted to a small set of genes nor uniform. Instead there are broad, varied, and...
Disentangling information flow in the Ras-cAMP signaling network (2006)
Carter, Gregory W., Rupp, Steffen, Fink, Gerald R., Galitski, Timothy
The perturbation of signal-transduction molecules elicits genomic-expression effects that are typically neither restricted to a small set of genes nor uniform. Instead there are broad, varied, and...
Feedback control of morphogenesis in fungi by aromatic alcohols (2006)
Many fungi undergo a developmental transition from a unicellular yeast form to an invasive filamentous form in response to environmental cues. Here we describe a quorum signaling pathway that links...
Feedback control of morphogenesis in fungi by aromatic alcohols (2006)
Many fungi undergo a developmental transition from a unicellular yeast form to an invasive filamentous form in response to environmental cues. Here we describe a quorum signaling pathway that links...
Intragenic tandem repeats generate functional variability (2005)
Jansen, An, Lewitter, Fran, Fink, Gerald R
Tandemly repeated DNA sequences are highly dynamic components of genomes. Most repeats are in intergenic regions, but some are in coding sequences or pseudogenes. In humans, expansion of intragenic...
Origins of variation in the fungal cell surface (2004)
Reynolds, Todd B, Fink, Gerald R
Whitehead Institute for Biomedical Research/MIT, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA.
Meimoun, Ariella, Holtzman, Tsvi, Weissman, Ziva, McBride, Helen J., Stillman, David J., Fink, Gerald R., ...
Gcn4, a yeast transcriptional activator that promotes the expression of amino acid and purine biosynthesis genes, is rapidly degraded in rich medium. Here we report that SCFCDC4, a recently...
Microarray Hybridization □d, Paul T. Spellman, Gavin Sherlock, Michael Q. Zhang, Vishwanath R, Kirk Anders, ...
this article is available at www.molbiolcell.org.
Methods in yeast genetics / course instructors Fred Sherman, Generald R. Fink, James B. Hicks (1982)
Sherman, Fred, Fink, Gerald R, Hicks, James B
Incluye bibliografía
This thesis is not available electronically or by photocopy. Please contact Archives and Special Collections at archives@amherst.edu for more information.
Meimoun, Ariella, Holtzman, Tsvi, Weissman, Ziva, McBride, Helen J., Stillman, David J., Fink, Gerald R., ...
Gcn4, a yeast transcriptional activator that promotes the expression of amino acid and purine biosynthesis genes, is rapidly degraded in rich medium. Here we report that SCFCDC4, a recently...
Gaxiola, Roberto A., Rao, Rajini, Sherman, Amir, Grisafi, Paula, Alper, Seth L., Fink, Gerald R.
Overexpression of the Arabidopsis thaliana vacuolar H+-pyrophosphatase (AVP1) confers salt tolerance to the salt-sensitive ena1 mutant of Saccharomyces cerevisiae. Suppression of salt sensitivity...
A Saccharomyces gene family involved in invasive growth, cell–cell adhesion, and mating
Guo, Bing, Styles, Cora A., Feng, Qinghua, Fink, Gerald R.
The cell wall of bakers' yeast contains a family of glycosyl-phosphatidylinositol (GPI)-linked glycoproteins of domain structure similar to the adhesins of pathogenic fungi. In wild-type cells each...
The yeast A kinases differentially regulate iron uptake and respiratory function
Robertson, Laura S., Causton, Helen C., Young, Richard A., Fink, Gerald R.
Yeast has three A kinase catalytic subunits, which have greater than 75% identity and are encoded by the TPK genes (TPK1, TPK2, and TPK3) [Toda, T., Cameron, S., Sass, P., Zoller, M. & Wigler, M....
The yeast CLC chloride channel functions in cation homeostasis
Gaxiola, Roberto A., Yuan, Daniel S., Klausner, Richard D., Fink, Gerald R.
A defect in the yeast GEF1 gene, a CLC chloride channel homolog leads to an iron requirement and cation sensitivity. The iron requirement is due to a failure to load Cu2+ onto a component of the iron...
A Myb homologue, ATR1, activates tryptophan gene expression in Arabidopsis
Bender, Judith, Fink, Gerald R.
In Arabidopsis thaliana, tryptophan pathway genes are induced in response to starvation, wounding, and pathogen attack, resulting in increased production of tryptophan and secondary metabolites...
The three yeast A kinases have specific signaling functions in pseudohyphal growth
Robertson, Laura S., Fink, Gerald R.
The three yeast A kinase catalytic subunit isoforms are redundant for viability. We demonstrate that they have dramatically different roles in pseudohyphal development: Tpk2 is essential, whereas...
Mösch, Hans-Ulrich, Kübler, Eric, Krappmann, Sven, Fink, Gerald R., Braus, Gerhard H.
The two highly conserved RAS genes of the budding yeast Saccharomyces cerevisiae are redundant for viability. Here we show that haploid invasive growth development depends on RAS2 but not RAS1. Ras1p...
Multiple Functions for Actin during Filamentous Growth of Saccharomyces cerevisiaeV⃞
Cali, Brian M., Doyle, Timothy C., Botstein, David, Fink, Gerald R.
Saccharomyces cerevisiae is dimorphic and switches from a yeast form to a pseudohyphal (PH) form when starved for nitrogen. PH cells are elongated, bud in a unipolar manner, and invade the agar...
Drought- and salt-tolerant plants result from overexpression of the AVP1 H+-pump
Gaxiola, Roberto A., Li, Jisheng, Undurraga, Soledad, Dang, Lien M., Allen, Gethyn J., Alper, Seth L., ...
Transgenic plants overexpressing the vacuolar H+-pyrophosphatase are much more resistant to high concentrations of NaCl and to water deprivation than the isogenic wild-type strains. These transgenic...
Mulet, Jose M., Leube, Martin P., Kron, Stephen J., Rios, Gabino, Fink, Gerald R., Serrano, Ramon
The regulation of intracellular ion concentrations is a fundamental property of living cells. Although many ion transporters have been identified, the systems that modulate their activity remain...
Klasson, Hanna, Fink, Gerald R., Ljungdahl, Per O.
Mutations in SSY1 and PTR3 were identified in a genetic selection for components required for the proper uptake and compartmentalization of histidine in Saccharomyces cerevisiae. Ssy1p is a unique...
Conservative Duplication of Spindle Poles during Meiosis in Saccharomyces cerevisiae
Wesp, Andreas, Prinz, Susanne, Fink, Gerald R.
During sporulation in diploid Saccharomyces cerevisiae, spindle pole bodies acquire the so-called meiotic plaque, a prerequisite for spore formation. Mpc70p is a component of the meiotic plaque and...
Multicellular Stalk-Like Structures in Saccharomyces cerevisiae
Engelberg, David, Mimran, Avishai, Martinetto, Horacio, Otto, Joel, Simchen, Giora, Karin, Michael, ...
Stalk formation is a novel pattern of multicellular organization. Yeast cells which survive UV irradiation form colonies that grow vertically to form very long (0.5 to 3.0 cm) and thin (0.5 to 4 mm...
Kaeberlein, Matt, Andalis, Alex A., Fink, Gerald R., Guarente, Leonard
Calorie restriction (CR) extends life span in many different organisms, including mammals. We describe here a novel pathway that extends the life span of Saccharomyces cerevisiae mother cells but...
Arabidopsis ALF5, a Multidrug Efflux Transporter Gene Family Member, Confers Resistance to Toxins
Diener, Andrew C., Gaxiola, Roberto A., Fink, Gerald R.
The Arabidopsis genome contains many gene families that are not found in the animal kingdom. One of these is the multidrug and toxic compound extrusion (MATE) family, which has homology with...
STEROL METHYLTRANSFERASE 1 Controls the Level of Cholesterol in Plants
Diener, Andrew C., Li, Haoxia, Zhou, Wen-xu, Whoriskey, Wendy J., Nes, W. David, Fink, Gerald R.
The side chain in plant sterols can have either a methyl or ethyl addition at carbon 24 that is absent in cholesterol. The ethyl addition is the product of two sequential methyl additions....
A Saccharomyces cerevisiae mutant with increased virulence
Wheeler, Robert T., Kupiec, Martin, Magnelli, Paula, Abeijon, Claudia, Fink, Gerald R.
Saccharomyces cerevisiae, bakers' yeast, is not a pathogen in healthy individuals, but is increasingly isolated from immunocompromised patients. The more frequent isolation of S. cerevisiae...
Goodman, Anya, Goode, Bruce L., Matsudaira, Paul, Fink, Gerald R.
Calponins and transgelins are members of a conserved family of actin-associated proteins widely expressed from yeast to humans. Although a role for calponin in muscle cells has been described, the...
Rubin-Bejerano, Ifat, Fraser, Iain, Grisafi, Paula, Fink, Gerald R.
The transcriptional profiles of yeast cells that have been phagocytosed by either human neutrophils or monocytes were compared by using whole genome arrays. After phagocytosis by neutrophils, both...
Kottom, Theodore J., Köhler, Julia R., Thomas, Charles F., Fink, Gerald R., Limper, Andrew H.
Pneumocystis carinii causes severe pneumonia in immunocompromised hosts. The binding of P. carinii to alveolar epithelial cells and extracellular matrix constituents such as fibronectin and...
NONSENSE MUTATIONS AFFECTING THE his4 ENZYME COMPLEX OF YEAST*
Shaffer, Barbara, Rytka, Joanna, Fink, Gerald R.
The his4 region of yeast contains the information necessary for the catalysis of three steps in the histidine biosynthetic pathway. The three activities specified by this region remain physically...
Simplified Method for Testing Mutagens in Saccharomyces
Fink, Gerald R., Lowenstein, Robert
Pretreatment of yeast auxotrophs with mutagen on complete medium stimulates the reversion frequency.
Yeast Killer Mutants with Altered Double-Stranded Ribonucleic Acid
Vodkin, Michael, Katterman, Frank, Fink, Gerald R.
Killer strains of Saccharomyces cerevisiae contain two species of double-stranded ribonucleic acid (dsRNA) with molecular weights estimated at 2.5 × 106 (L) and 1.4 × 106 (M). The M component...
Luschnig, Christian, Gaxiola, Roberto A., Grisafi, Paula, Fink, Gerald R.
The EIR1 gene of Arabidopsis is a member of a family of plant genes with similarities to bacterial membrane transporters. This gene is expressed only in the root, which is consistent with the...
The plant hormone indoleacetic acid induces invasive growth in Saccharomyces cerevisiae
Prusty, Reeta, Grisafi, Paula, Fink, Gerald R.
Fungi must recognize plant-specific signals to initiate subsequent morphogenetic events such as filamentation that lead to infection. Here we show that the plant hormone indoleacetic acid (IAA)...
Tyrosol is a quorum-sensing molecule in Candida albicans
Chen, Hao, Fujita, Masaki, Feng, Qinghua, Clardy, Jon, Fink, Gerald R.
The human fungal pathogen Candida albicans shows a significant lag in growth when diluted into fresh minimal medium. This lag is abolished by the addition of conditioned medium from a high-density...
Curing of a Killer Factor in Saccharomyces cerevisiae
Fink, Gerald R., Styles, Cora Ann
Many standard laboratory stocks of yeast are able to kill other yeast strains. This property has not been generally recognized because killing is observed only at low pH and not at the pH of standard...
A Nucleic Acid Associated with a Killer Strain of Yeast
Vodkin, Michael H., Fink, Gerald R.
A crude membrane fraction was isolated from both killer M(k) and isogenic nonkiller M(o) strains of yeast labeled with [3H]adenine. Nucleic acids were extracted from this fraction and centrifuged to...
Liu, Shihui, Milne, G. Todd, Kuremsky, Jeffrey G., Fink, Gerald R., Leppla, Stephen H.
Diphthamide, a posttranslational modification of translation elongation factor 2 that is conserved in all eukaryotes and archaebacteria and is the target of diphtheria toxin, is formed in yeast by...
Transcriptional Response of Candida albicans upon Internalization by Macrophages
Lorenz, Michael C., Bender, Jennifer A., Fink, Gerald R.
The opportunistic fungal pathogen Candida albicans is both a benign gut commensal and a frequently fatal systemic pathogen. The interaction of C. albicans with the host's innate immune system is the...
Plant growth homeostasis is controlled by the Arabidopsis BON1 and BAP1 genes
Hua, Jian, Grisafi, Paula, Cheng, Shu-Hua, Fink, Gerald R.
Wild-type Arabidopsis plants maintain a relatively constant size over a wide range of temperatures. Here we show that this homeostasis requires the BONZAI1 (BON1) gene because bon1 null mutants make...
A Drug-Sensitive Genetic Network Masks Fungi from the Immune System
Wheeler, Robert T, Fink, Gerald R
Fungal pathogens can be recognized by the immune system via their β-glucan, a potent proinflammatory molecule that is present at high levels but is predominantly buried beneath a mannoprotein coat...
Intragenic tandem repeats generate functional variability
Verstrepen, Kevin J., Jansen, An, Lewitter, Fran, Fink, Gerald R.
Tandemly repeated DNA sequences are highly dynamic components of genomes1. Most repeats are in intergenic regions, but some are found within coding sequences or pseudogenes2. In humans, expansion of...
Mutations Affecting Ty-Mediated Expression of the HIS4 Gene of SACCHAROMYCES CEREVISIAE
Winston, Fred, Chaleff, Deborah T., Valent, Barbara, Fink, Gerald R.
We have identified mutations in seven unlinked genes (SPT genes) that affect the phenotypes of Ty and δ insertion mutations in the 5' noncoding region of the HIS4 gene of S. cerevisiae. Spt mutants...
Meiotic Recombination between Duplicated Genetic Elements in SACCHAROMYCES CEREVISIAE
Jackson, Jennifer A., Fink, Gerald R.
We have studied the meiotic recombination behavior of strains carrying two types of duplications of an 18.6-kilobase HIS4 BamHI fragment. The first type is a direct duplication of the HIS4 BamHI...
Temperature-Sensitive Nonsense Suppressors in Yeast
Rasse-Messenguy, Francine, Fink, Gerald R.
A single mutation leads to temperature-sensitive amber and ochre suppression in yeast. This mutation maps in or near the SUP4 gene on chromosome X.
Sweeney, T. Kevin, Tate, Ann, Fink, Gerald R.
Killer strains contain two double stranded RNAs, L and M. The M dsRNA appears to be necessary for production of a toxin and for resistance to that toxin. Mutant strains have been found that are...
Frameshifts and Frameshift Suppressors in SACCHAROMYCES CEREVISIAE
Culbertson, Michael R., Charnas, Lawrence, Johnson, M. Tina, Fink, Gerald R.
Using ICR-170 as a mutagen, we have induced a set of mutations in yeast which exhibit behavior similar to that shown for bacterial frameshift mutations. Our genetic study shows that these mutations...
Frameshift Suppression in SACCHAROMYCES CEREVISIAE. II. Genetic Properties of Group II Suppressors
Culbertson, Michael R., Underbrink, Karen M., Fink, Gerald R.
Suppressors of ICR-induced mutations that exhibit behavior similar to bacterial frameshift suppressors have been identified in the yeast Saccharomyces cerevisiae. The yeast suppressors have been...
Cummins, Claudia M., Gaber, Richard F., Culbertson, Michael R., Mann, Richard, Fink, Gerald R.
Suppressors of ICR-induced mutations that exhibit behavior similar to bacterial frameshift suppressors have been identified in the yeast Saccharomyces cerevisiae. The yeast suppressors have been...
Saccharomyces cerevisiae SSD1-V confers longevity by a Sir2p-independent mechanism.
Kaeberlein, Matt, Andalis, Alex A, Liszt, Gregory B, Fink, Gerald R, Guarente, Leonard
The SSD1 gene of Saccharomyces cerevisiae is a polymorphic locus that affects diverse cellular processes including cell integrity, cell cycle progression, and growth at high temperature. We show here...
Defects arising from whole-genome duplications in Saccharomyces cerevisiae.
Andalis, Alex A, Storchova, Zuzana, Styles, Cora, Galitski, Timothy, Pellman, David, Fink, Gerald R
Comparisons among closely related species have led to the proposal that the duplications found in many extant genomes are the remnants of an ancient polyploidization event, rather than a result of...
Disentangling information flow in the Ras-cAMP signaling network
Carter, Gregory W., Rupp, Steffen, Fink, Gerald R., Galitski, Timothy
The perturbation of signal-transduction molecules elicits genomic-expression effects that are typically neither restricted to a small set of genes nor uniform. Instead there are broad, varied, and...
Feedback control of morphogenesis in fungi by aromatic alcohols
Many fungi undergo a developmental transition from a unicellular yeast form to an invasive filamentous form in response to environmental cues. Here we describe a quorum signaling pathway that links...
Meimoun, Ariella, Holtzman, Tsvi, Weissman, Ziva, McBride, Helen J., Stillman, David J., Fink, Gerald R., ...
Gcn4, a yeast transcriptional activator that promotes the expression of amino acid and purine biosynthesis genes, is rapidly degraded in rich medium. Here we report that SCFCDC4, a recently...
Gaxiola, Roberto A., Rao, Rajini, Sherman, Amir, Grisafi, Paula, Alper, Seth L., Fink, Gerald R.
Overexpression of the Arabidopsis thaliana vacuolar H+-pyrophosphatase (AVP1) confers salt tolerance to the salt-sensitive ena1 mutant of Saccharomyces cerevisiae. Suppression of salt sensitivity...
A Saccharomyces gene family involved in invasive growth, cell–cell adhesion, and mating
Guo, Bing, Styles, Cora A., Feng, Qinghua, Fink, Gerald R.
The cell wall of bakers' yeast contains a family of glycosyl-phosphatidylinositol (GPI)-linked glycoproteins of domain structure similar to the adhesins of pathogenic fungi. In wild-type cells each...
The yeast A kinases differentially regulate iron uptake and respiratory function
Robertson, Laura S., Causton, Helen C., Young, Richard A., Fink, Gerald R.
Yeast has three A kinase catalytic subunits, which have greater than 75% identity and are encoded by the TPK genes (TPK1, TPK2, and TPK3) [Toda, T., Cameron, S., Sass, P., Zoller, M. & Wigler, M....
The yeast CLC chloride channel functions in cation homeostasis
Gaxiola, Roberto A., Yuan, Daniel S., Klausner, Richard D., Fink, Gerald R.
A defect in the yeast GEF1 gene, a CLC chloride channel homolog leads to an iron requirement and cation sensitivity. The iron requirement is due to a failure to load Cu2+ onto a component of the iron...
A Myb homologue, ATR1, activates tryptophan gene expression in Arabidopsis
Bender, Judith, Fink, Gerald R.
In Arabidopsis thaliana, tryptophan pathway genes are induced in response to starvation, wounding, and pathogen attack, resulting in increased production of tryptophan and secondary metabolites...
The three yeast A kinases have specific signaling functions in pseudohyphal growth
Robertson, Laura S., Fink, Gerald R.
The three yeast A kinase catalytic subunit isoforms are redundant for viability. We demonstrate that they have dramatically different roles in pseudohyphal development: Tpk2 is essential, whereas...
Mösch, Hans-Ulrich, Kübler, Eric, Krappmann, Sven, Fink, Gerald R., Braus, Gerhard H.
The two highly conserved RAS genes of the budding yeast Saccharomyces cerevisiae are redundant for viability. Here we show that haploid invasive growth development depends on RAS2 but not RAS1. Ras1p...
Multiple Functions for Actin during Filamentous Growth of Saccharomyces cerevisiaeV⃞
Cali, Brian M., Doyle, Timothy C., Botstein, David, Fink, Gerald R.
Saccharomyces cerevisiae is dimorphic and switches from a yeast form to a pseudohyphal (PH) form when starved for nitrogen. PH cells are elongated, bud in a unipolar manner, and invade the agar...
Drought- and salt-tolerant plants result from overexpression of the AVP1 H+-pump
Gaxiola, Roberto A., Li, Jisheng, Undurraga, Soledad, Dang, Lien M., Allen, Gethyn J., Alper, Seth L., ...
Transgenic plants overexpressing the vacuolar H+-pyrophosphatase are much more resistant to high concentrations of NaCl and to water deprivation than the isogenic wild-type strains. These transgenic...
Mulet, Jose M., Leube, Martin P., Kron, Stephen J., Rios, Gabino, Fink, Gerald R., Serrano, Ramon
The regulation of intracellular ion concentrations is a fundamental property of living cells. Although many ion transporters have been identified, the systems that modulate their activity remain...
Klasson, Hanna, Fink, Gerald R., Ljungdahl, Per O.
Mutations in SSY1 and PTR3 were identified in a genetic selection for components required for the proper uptake and compartmentalization of histidine in Saccharomyces cerevisiae. Ssy1p is a unique...
Conservative Duplication of Spindle Poles during Meiosis in Saccharomyces cerevisiae
Wesp, Andreas, Prinz, Susanne, Fink, Gerald R.
During sporulation in diploid Saccharomyces cerevisiae, spindle pole bodies acquire the so-called meiotic plaque, a prerequisite for spore formation. Mpc70p is a component of the meiotic plaque and...
Multicellular Stalk-Like Structures in Saccharomyces cerevisiae
Engelberg, David, Mimran, Avishai, Martinetto, Horacio, Otto, Joel, Simchen, Giora, Karin, Michael, ...
Stalk formation is a novel pattern of multicellular organization. Yeast cells which survive UV irradiation form colonies that grow vertically to form very long (0.5 to 3.0 cm) and thin (0.5 to 4 mm...
Kaeberlein, Matt, Andalis, Alex A., Fink, Gerald R., Guarente, Leonard
Calorie restriction (CR) extends life span in many different organisms, including mammals. We describe here a novel pathway that extends the life span of Saccharomyces cerevisiae mother cells but...
Arabidopsis ALF5, a Multidrug Efflux Transporter Gene Family Member, Confers Resistance to Toxins
Diener, Andrew C., Gaxiola, Roberto A., Fink, Gerald R.
The Arabidopsis genome contains many gene families that are not found in the animal kingdom. One of these is the multidrug and toxic compound extrusion (MATE) family, which has homology with...
STEROL METHYLTRANSFERASE 1 Controls the Level of Cholesterol in Plants
Diener, Andrew C., Li, Haoxia, Zhou, Wen-xu, Whoriskey, Wendy J., Nes, W. David, Fink, Gerald R.
The side chain in plant sterols can have either a methyl or ethyl addition at carbon 24 that is absent in cholesterol. The ethyl addition is the product of two sequential methyl additions....
A Saccharomyces cerevisiae mutant with increased virulence
Wheeler, Robert T., Kupiec, Martin, Magnelli, Paula, Abeijon, Claudia, Fink, Gerald R.
Saccharomyces cerevisiae, bakers' yeast, is not a pathogen in healthy individuals, but is increasingly isolated from immunocompromised patients. The more frequent isolation of S. cerevisiae...
Goodman, Anya, Goode, Bruce L., Matsudaira, Paul, Fink, Gerald R.
Calponins and transgelins are members of a conserved family of actin-associated proteins widely expressed from yeast to humans. Although a role for calponin in muscle cells has been described, the...
Rubin-Bejerano, Ifat, Fraser, Iain, Grisafi, Paula, Fink, Gerald R.
The transcriptional profiles of yeast cells that have been phagocytosed by either human neutrophils or monocytes were compared by using whole genome arrays. After phagocytosis by neutrophils, both...
Kottom, Theodore J., Köhler, Julia R., Thomas, Charles F., Fink, Gerald R., Limper, Andrew H.
Pneumocystis carinii causes severe pneumonia in immunocompromised hosts. The binding of P. carinii to alveolar epithelial cells and extracellular matrix constituents such as fibronectin and...
NONSENSE MUTATIONS AFFECTING THE his4 ENZYME COMPLEX OF YEAST*
Shaffer, Barbara, Rytka, Joanna, Fink, Gerald R.
The his4 region of yeast contains the information necessary for the catalysis of three steps in the histidine biosynthetic pathway. The three activities specified by this region remain physically...
Simplified Method for Testing Mutagens in Saccharomyces
Fink, Gerald R., Lowenstein, Robert
Pretreatment of yeast auxotrophs with mutagen on complete medium stimulates the reversion frequency.
Yeast Killer Mutants with Altered Double-Stranded Ribonucleic Acid
Vodkin, Michael, Katterman, Frank, Fink, Gerald R.
Killer strains of Saccharomyces cerevisiae contain two species of double-stranded ribonucleic acid (dsRNA) with molecular weights estimated at 2.5 × 106 (L) and 1.4 × 106 (M). The M component...
Plant growth homeostasis is controlled by the Arabidopsis BON1 and BAP1 genes
Hua, Jian, Grisafi, Paula, Cheng, Shu-Hua, Fink, Gerald R.
Wild-type Arabidopsis plants maintain a relatively constant size over a wide range of temperatures. Here we show that this homeostasis requires the BONZAI1 (BON1) gene because bon1 null mutants make...
Luschnig, Christian, Gaxiola, Roberto A., Grisafi, Paula, Fink, Gerald R.
The EIR1 gene of Arabidopsis is a member of a family of plant genes with similarities to bacterial membrane transporters. This gene is expressed only in the root, which is consistent with the...
The plant hormone indoleacetic acid induces invasive growth in Saccharomyces cerevisiae
Prusty, Reeta, Grisafi, Paula, Fink, Gerald R.
Fungi must recognize plant-specific signals to initiate subsequent morphogenetic events such as filamentation that lead to infection. Here we show that the plant hormone indoleacetic acid (IAA)...
Tyrosol is a quorum-sensing molecule in Candida albicans
Chen, Hao, Fujita, Masaki, Feng, Qinghua, Clardy, Jon, Fink, Gerald R.
The human fungal pathogen Candida albicans shows a significant lag in growth when diluted into fresh minimal medium. This lag is abolished by the addition of conditioned medium from a high-density...
Curing of a Killer Factor in Saccharomyces cerevisiae
Fink, Gerald R., Styles, Cora Ann
Many standard laboratory stocks of yeast are able to kill other yeast strains. This property has not been generally recognized because killing is observed only at low pH and not at the pH of standard...
A Nucleic Acid Associated with a Killer Strain of Yeast
Vodkin, Michael H., Fink, Gerald R.
A crude membrane fraction was isolated from both killer M(k) and isogenic nonkiller M(o) strains of yeast labeled with [3H]adenine. Nucleic acids were extracted from this fraction and centrifuged to...
Liu, Shihui, Milne, G. Todd, Kuremsky, Jeffrey G., Fink, Gerald R., Leppla, Stephen H.
Diphthamide, a posttranslational modification of translation elongation factor 2 that is conserved in all eukaryotes and archaebacteria and is the target of diphtheria toxin, is formed in yeast by...
Transcriptional Response of Candida albicans upon Internalization by Macrophages
Lorenz, Michael C., Bender, Jennifer A., Fink, Gerald R.
The opportunistic fungal pathogen Candida albicans is both a benign gut commensal and a frequently fatal systemic pathogen. The interaction of C. albicans with the host's innate immune system is the...
Mutations Affecting Ty-Mediated Expression of the HIS4 Gene of SACCHAROMYCES CEREVISIAE
Winston, Fred, Chaleff, Deborah T., Valent, Barbara, Fink, Gerald R.
We have identified mutations in seven unlinked genes (SPT genes) that affect the phenotypes of Ty and δ insertion mutations in the 5' noncoding region of the HIS4 gene of S. cerevisiae. Spt mutants...
Meiotic Recombination between Duplicated Genetic Elements in SACCHAROMYCES CEREVISIAE
Jackson, Jennifer A., Fink, Gerald R.
We have studied the meiotic recombination behavior of strains carrying two types of duplications of an 18.6-kilobase HIS4 BamHI fragment. The first type is a direct duplication of the HIS4 BamHI...
Temperature-Sensitive Nonsense Suppressors in Yeast
Rasse-Messenguy, Francine, Fink, Gerald R.
A single mutation leads to temperature-sensitive amber and ochre suppression in yeast. This mutation maps in or near the SUP4 gene on chromosome X.
Sweeney, T. Kevin, Tate, Ann, Fink, Gerald R.
Killer strains contain two double stranded RNAs, L and M. The M dsRNA appears to be necessary for production of a toxin and for resistance to that toxin. Mutant strains have been found that are...
Frameshifts and Frameshift Suppressors in SACCHAROMYCES CEREVISIAE
Culbertson, Michael R., Charnas, Lawrence, Johnson, M. Tina, Fink, Gerald R.
Using ICR-170 as a mutagen, we have induced a set of mutations in yeast which exhibit behavior similar to that shown for bacterial frameshift mutations. Our genetic study shows that these mutations...
Frameshift Suppression in SACCHAROMYCES CEREVISIAE. II. Genetic Properties of Group II Suppressors
Culbertson, Michael R., Underbrink, Karen M., Fink, Gerald R.
Suppressors of ICR-induced mutations that exhibit behavior similar to bacterial frameshift suppressors have been identified in the yeast Saccharomyces cerevisiae. The yeast suppressors have been...
Cummins, Claudia M., Gaber, Richard F., Culbertson, Michael R., Mann, Richard, Fink, Gerald R.
Suppressors of ICR-induced mutations that exhibit behavior similar to bacterial frameshift suppressors have been identified in the yeast Saccharomyces cerevisiae. The yeast suppressors have been...
A Drug-Sensitive Genetic Network Masks Fungi from the Immune System
Wheeler, Robert T, Fink, Gerald R
Fungal pathogens can be recognized by the immune system via their β-glucan, a potent proinflammatory molecule that is present at high levels but is predominantly buried beneath a mannoprotein coat...
Disentangling information flow in the Ras-cAMP signaling network
Carter, Gregory W., Rupp, Steffen, Fink, Gerald R., Galitski, Timothy
The perturbation of signal-transduction molecules elicits genomic-expression effects that are typically neither restricted to a small set of genes nor uniform. Instead there are broad, varied, and...
Saccharomyces cerevisiae SSD1-V confers longevity by a Sir2p-independent mechanism.
Kaeberlein, Matt, Andalis, Alex A, Liszt, Gregory B, Fink, Gerald R, Guarente, Leonard
The SSD1 gene of Saccharomyces cerevisiae is a polymorphic locus that affects diverse cellular processes including cell integrity, cell cycle progression, and growth at high temperature. We show here...
Defects arising from whole-genome duplications in Saccharomyces cerevisiae.
Andalis, Alex A, Storchova, Zuzana, Styles, Cora, Galitski, Timothy, Pellman, David, Fink, Gerald R
Comparisons among closely related species have led to the proposal that the duplications found in many extant genomes are the remnants of an ancient polyploidization event, rather than a result of...
Feedback control of morphogenesis in fungi by aromatic alcohols
Many fungi undergo a developmental transition from a unicellular yeast form to an invasive filamentous form in response to environmental cues. Here we describe a quorum signaling pathway that links...
Genes with internal repeats require the THO complex for transcription
Voynov, Vladimir, Verstrepen, Kevin J., Jansen, An, Runner, Vanessa M., Buratowski, Stephen, Fink, Gerald R.
The evolutionarily conserved multisubunit THO complex, which is recruited to actively transcribed genes, is required for the efficient expression of FLO11 and other yeast genes that have long...
Genetic Architecture of Hsp90-Dependent Drug Resistance▿
Cowen, Leah E., Carpenter, Anne E., Matangkasombut, Oranart, Fink, Gerald R., Lindquist, Susan
Hsp90 potentiates the evolution of azole resistance in the model yeast Saccharomyces cerevisiae and the opportunistic pathogen Candida albicans via calcineurin. Here, we explored effectors downstream...
Coding Tandem Repeats Generate Diversity in Aspergillus fumigatus Genes▿ †
Levdansky, Emma, Romano, Jacob, Shadkchan, Yona, Sharon, Haim, Verstrepen, Kevin J., Fink, Gerald R., ...
Genes containing multiple coding mini- and microsatellite repeats are highly dynamic components of genomes. Frequent recombination events within these tandem repeats lead to changes in repeat...
Zumbuehl, Andreas, Ferreira, Lino, Kuhn, Duncan, Astashkina, Anna, Long, Lisa, Yeo, Yoon, ...
Fungi are increasingly identified as major pathogens in bloodstream infections, often involving indwelling devices. Materials with antifungal properties may provide an important deterrent to these...
Mat Formation in Saccharomyces cerevisiae Requires Nutrient and pH Gradients▿
Reynolds, Todd B., Jansen, An, Peng, Xin, Fink, Gerald R.
The ability of Saccharomyces cerevisiae to form morphologically complex colony-like structures called mats requires expression of the cell surface glycoprotein Flo11p and growth on a semisolid...
Polyploids require Bik1 for kinetochore–microtubule attachment
Lin, Haijiang, De Carvalho, Pedro, Kho, David, Tai, Chin-Yin, Pierre, Philippe, Fink, Gerald R., ...
The attachment of kinetochores to spindle microtubules (MTs) is essential for maintaining constant ploidy in eukaryotic cells. Here, biochemical and imaging data is presented demonstrating that the...
Dynamic, Morphotype-Specific Candida albicans β-Glucan Exposure during Infection and Drug Treatment
Wheeler, Robert T., Kombe, Diana, Agarwala, Sudeep D., Fink, Gerald R.
Candida albicans, a clinically important dimorphic fungal pathogen that can evade immune attack by masking its cell wall β-glucan from immune recognition, mutes protective host responses mediated by...