Kae, Helmut, Kortholt, Arjan, Rehmann, Holger, Insall, Robert H., Spiegelman, George B., ...
In general, mammalian Ras guanine nucleotide exchange factors (RasGEFs) show little substrate specificity, although they are often thought to regulate specific pathways. Here, we provide in vitro and...
Kortholt, Arjan, Rehmann, Holger, Kae, Helmut, Bosgraaf, Leonard, Keizer-Gunnink, Ineke, Weeks, Gerald, ...
The regulation of cell polarity plays an important role in chemotaxis. GbpD, a putative nucleotide exchange factor for small G-proteins of the Ras family, has been implicated in adhesion, cell...
Korholt, Arjan, Rehmann, Holger, Kae, Helmut, Bosgraaf, Leonard, Keizer-Gunnink, Ineke, Weeks, Gerald, ...
A novel DictyosteliumRasGEF required for chemotaxis and development (2005)
Arigoni, Maddalena, Bracco, Enrico, Lusche, Daniel F, Kae, Helmut, Weeks, Gerald, Bozzaro, Salvatore
Abstract Background Ras proteins are guanine-nucleotide-binding enzymes that couple cell surface receptors to intracellular signaling pathways controlling cell proliferation and differentiation, both...
Dictyostelium RasD is required for normal phototaxis, but not differentiation (2000)
Wilkins, Andrew, Khosla, Meenal, Fraser, Derek J., Spiegelman, George B., Fisher, Paul R., Weeks, Gerald, ...
Seastone, David J., Zhang, Linyi, Buczynski, Greg, Rebstein, Patrick, Weeks, Gerald, Spiegelman, George, ...
The function of the small-Mr Ras-like GTPase Rap1 remains largely unknown, but this protein has been demonstrated to regulate cortical actin-based morphologic changes in Dictyostelium and the...
RasC is required for optimal activation of adenylyl cyclase and Akt/PKB during aggregation
Lim, Chinten James, Spiegelman, George B., Weeks, Gerald
Disruption of Dictyostelium rasC, encoding a Ras subfamily protein, generated cells incapable of aggregation. While rasC expression is enriched in a cell type-specific manner during post-aggregative...
Weeks, Gerald, Shapiro, Martin, Burns, R. O., Wakil, Salih J.
Escherichia coli grows on long-chain fatty acids after a distinct lag phase. Cells, preadapted to palmitate, grow immediately on fatty acids, indicating that fatty acid oxidation in this bacterium is...
Dictyostelium RasD is required for normal phototaxis, but not differentiation
Wilkins, Andrew, Khosla, Meenal, Fraser, Derek J., Spiegelman, George B., Fisher, Paul R., Weeks, Gerald, ...
RasD, a Dictyostelium homolog of mammalian Ras, is maximally expressed during the multicellular stage of development. Normal Dictyostelium aggregates are phototactic and thermotactic, moving towards...
Wessels, Deborah, Brincks, Rebecca, Kuhl, Spencer, Stepanovic, Vesna, Daniels, Karla J., Weeks, Gerald, ...
To define the role that RasC plays in motility and chemotaxis, the behavior of a rasC null mutant, rasC−, in buffer and in response to the individual spatial, temporal, and concentration components...
A Ras subfamily GTPase shows cell cycle-dependent nuclear localization
Sutherland, Brent W., Spiegelman, George B., Weeks, Gerald
Previously characterized Ras subfamily proteins have been found to be predominantly associated with the plasma membrane where they function in signal transduction pathways to convey extracellular...
Chemoattractant-induced Ras activation during Dictyostelium aggregation
Kae, Helmut, Lim, Chinten James, Spiegelman, George B, Weeks, Gerald
Ras proteins are highly conserved molecular switches that regulate cellular response to external stimuli. Dictyostelium discoideum contains an extensive family of Ras proteins that function in...
A novel Dictyostelium RasGEF required for chemotaxis and development
Arigoni, Maddalena, Bracco, Enrico, Lusche, Daniel F, Kae, Helmut, Weeks, Gerald, Bozzaro, Salvatore
Bolourani, Parvin, Spiegelman, George B., Weeks, Gerald
On starvation, the cellular slime mold Dictyostelium discoideum initiates a program of development leading to formation of multicellular structures. The initial cell aggregation requires chemotaxis...
Seastone, David J., Zhang, Linyi, Buczynski, Greg, Rebstein, Patrick, Weeks, Gerald, Spiegelman, George, ...
The function of the small-Mr Ras-like GTPase Rap1 remains largely unknown, but this protein has been demonstrated to regulate cortical actin-based morphologic changes in Dictyostelium and the...
RasC is required for optimal activation of adenylyl cyclase and Akt/PKB during aggregation
Lim, Chinten James, Spiegelman, George B., Weeks, Gerald
Disruption of Dictyostelium rasC, encoding a Ras subfamily protein, generated cells incapable of aggregation. While rasC expression is enriched in a cell type-specific manner during post-aggregative...
Weeks, Gerald, Shapiro, Martin, Burns, R. O., Wakil, Salih J.
Escherichia coli grows on long-chain fatty acids after a distinct lag phase. Cells, preadapted to palmitate, grow immediately on fatty acids, indicating that fatty acid oxidation in this bacterium is...
Dictyostelium RasD is required for normal phototaxis, but not differentiation
Wilkins, Andrew, Khosla, Meenal, Fraser, Derek J., Spiegelman, George B., Fisher, Paul R., Weeks, Gerald, ...
RasD, a Dictyostelium homolog of mammalian Ras, is maximally expressed during the multicellular stage of development. Normal Dictyostelium aggregates are phototactic and thermotactic, moving towards...
Wessels, Deborah, Brincks, Rebecca, Kuhl, Spencer, Stepanovic, Vesna, Daniels, Karla J., Weeks, Gerald, ...
To define the role that RasC plays in motility and chemotaxis, the behavior of a rasC null mutant, rasC−, in buffer and in response to the individual spatial, temporal, and concentration components...
A Ras subfamily GTPase shows cell cycle-dependent nuclear localization
Sutherland, Brent W., Spiegelman, George B., Weeks, Gerald
Previously characterized Ras subfamily proteins have been found to be predominantly associated with the plasma membrane where they function in signal transduction pathways to convey extracellular...
Chemoattractant-induced Ras activation during Dictyostelium aggregation
Kae, Helmut, Lim, Chinten James, Spiegelman, George B, Weeks, Gerald
Ras proteins are highly conserved molecular switches that regulate cellular response to external stimuli. Dictyostelium discoideum contains an extensive family of Ras proteins that function in...
A novel Dictyostelium RasGEF required for chemotaxis and development
Arigoni, Maddalena, Bracco, Enrico, Lusche, Daniel F, Kae, Helmut, Weeks, Gerald, Bozzaro, Salvatore
Bolourani, Parvin, Spiegelman, George B., Weeks, Gerald
On starvation, the cellular slime mold Dictyostelium discoideum initiates a program of development leading to formation of multicellular structures. The initial cell aggregation requires chemotaxis...
Rap1 controls cell adhesion and cell motility through the regulation of myosin II
Jeon, Taeck J., Lee, Dai-Jen, Merlot, Sylvain, Weeks, Gerald, Firtel, Richard A.
We have investigated the role of Rap1 in controlling chemotaxis and cell adhesion in Dictyostelium discoideum. Rap1 is activated rapidly in response to chemoattractant stimulation, and activated Rap1...
Kae, Helmut, Kortholt, Arjan, Rehmann, Holger, Insall, Robert H, Spiegelman, George B, ...
In general, mammalian Ras guanine nucleotide exchange factors (RasGEFs) show little substrate specificity, although they are often thought to regulate specific pathways. Here, we provide in vitro and...
Dictyostelium RasG Is Required for Normal Motility and Cytokinesis, But Not Growth
Tuxworth, Richard I., Cheetham, Janet L., Machesky, Laura M., Spiegelmann, George B., Weeks, Gerald, Insall, Robert H.
RasG is the most abundant Ras protein in growing Dictyostelium cells and the closest relative of mammalian Ras proteins. We have generated null mutants in which expression of RasG is completely...
Regulation of Rap1 activity by RapGAP1 controls cell adhesion at the front of chemotaxing cells
Jeon, Taeck J., Lee, Dai-Jen, Lee, Susan, Weeks, Gerald, Firtel, Richard A.
Spatial and temporal regulation of Rap1 is required for proper myosin assembly and cell adhesion during cell migration in Dictyostelium discoideum. Here, we identify a Rap1 guanosine...
Parkinson, Katie, Bolourani, Parvin, Traynor, David, Aldren, Nicola L., Kay, Robert R., Weeks, Gerald, ...
Regulated cell adhesion and motility have important roles during growth, development and tissue homeostasis. Consequently, great efforts have been made to identify genes that control these processes....