Scientific Commons: Gillis R. Otten

Increased Expression and Immunogenicity of Sequence-Modified Human Immunodeficiency Virus Type 1 gag Gene

Zur Megede, Jan, Chen, Min-Chao, Doe, Barbara, Schaefer, Mary, Greer, Catherine E., Selby, Mark, ...

A major challenge for the next generation of human immunodeficiency virus (HIV) vaccines is the induction of potent, broad, and durable cellular immune responses. The structural protein Gag is highly...

Infection of Human Dendritic Cells by a Sindbis Virus Replicon Vector Is Determined by a Single Amino Acid Substitution in the E2 Glycoprotein

Gardner, Jason P., Frolov, Ilya, Perri, Silvia, Ji, Yaying, MacKichan, Mary Lee, Zur Megede, Jan, ...

The ability to target antigen-presenting cells with vectors encoding desired antigens holds the promise of potent prophylactic and therapeutic vaccines for infectious diseases and cancer. Toward this...

Induction of Potent Immune Responses by Cationic Microparticles with Adsorbed Human Immunodeficiency Virus DNA Vaccines

O'Hagan, Derek, Singh, Manmohan, Ugozzoli, Mildred, Wild, Carl, Barnett, Susan, Chen, Minchao, ...

The effectiveness of cationic microparticles with adsorbed DNA at inducing immune responses was investigated in mice, guinea pigs, and rhesus macaques. Plasmid DNA vaccines encoding human...

An Alphavirus Replicon Particle Chimera Derived from Venezuelan Equine Encephalitis and Sindbis Viruses Is a Potent Gene-Based Vaccine Delivery Vector

Perri, Silvia, Greer, Catherine E., Thudium, Kent, Doe, Barbara, Legg, Harold, Liu, Hong, ...

Alphavirus replicon particle-based vaccine vectors derived from Sindbis virus (SIN), Semliki Forest virus, and Venezuelan equine encephalitis virus (VEE) have been shown to induce robust...

Enhanced Potency of Plasmid DNA Microparticle Human Immunodeficiency Virus Vaccines in Rhesus Macaques by Using a Priming-Boosting Regimen with Recombinant Proteins

Otten, Gillis R., Schaefer, Mary, Doe, Barbara, Liu, Hong, Srivastava, Indresh, Zur Megede, Jan, ...

DNA vaccines have been used widely in experimental primate models of human immunodeficiency virus (HIV), but their effectiveness has been limited. In this study, we evaluated three technologies for...

Expression and Immunogenicity of Sequence-Modified Human Immunodeficiency Virus Type 1 Subtype B pol and gagpol DNA Vaccines

Zur Megede, Jan, Otten, Gillis R., Doe, Barbara, Liu, Hong, Leung, Louisa, Ulmer, Jeffrey B., ...

Control of the worldwide AIDS pandemic may require not only preventive but also therapeutic immunization strategies. To meet this challenge, the next generation of human immunodeficiency virus type 1...