Giovanni E. Mann

Vascular NAD(P)H oxidase activation in diabetes: a double-edged sword in redox signalling (2009)

Gao, Ling, Mann, Giovanni E.

Oxidative stress mediated by hyperglycaemia-induced generation of reactive oxygen species (ROS) contributes significantly to the development and progression of diabetes and related vascular...

Endothelial dysfunction and reduced antioxidant protection in an animal model of the developmental origins of cardiovascular disease (2008)

Rodford, Joanne L., Torrens, Christopher, Siow, Richard C.M., Mann, Giovanni E., Hanson, Mark A., Clough, Geraldine F.

Endothelial dysfunction underlies cardiovascular disease (CVD) in humans and is reported in animal models of developmental origins of such disease. We have investigated whether impaired antioxidant...

Activation of endothelial nitric oxide synthase by dietary isoflavones: Role of NO in Nrf2-mediated antioxidant gene expression (2007)

Mann, Giovanni E., Rowlands, David J., Li, Francois Y.L., De Winter, Patricia, Siow, Richard C.M.

The endothelium plays a key role in the maintenance of vascular homeostasis, and increased oxidative stress in vascular disease leads to reduced nitric oxide bioavailability and impaired...

Platelet nitric oxide synthesis in uremia and malnutrition: A role for L-arginine supplementation in vascular protection? (2007)

Brunini, Tatiana M.C., Mendes-Ribeiro, Antônio C., Ellory, John C., Mann, Giovanni E.

L-arginine is the physiological precursor for nitric oxide (NO) synthesis, and availability and transport of L-arginine modulate the rates of NO biosynthesis in circulating blood cells and the...

In utero protein restriction leads to a down-regulation of antioxidant gene expression in adult male offspring (2006)

Rodford, Joanne, Li, Francois Y., Siow, Richard C.M., Hanson, Mark A., Mann, Giovanni E., Clough, Geraldine F.

In rats, restriction of dietary protein during pregnancy leads to raised blood pressure, impaired vasodilatation (Brawley et al. 2003) and increased oxidative damage (increased protein carbonyl...

Acute activation of Maxi-K channels (hSlo) by estradiol binding to the beta subunit (1999)

Valverde, Miguel A., Rojas, Patricio, Amigo, Julio, Cosmelli, Diego, Orio, Patricio, Bahamonde, María I., ...

Maxi-K channels consist of a pore-forming alpha subunit and a regulatory beta subunit, which confers the channel with a higher Ca2+ sensitivity. Estradiol bound to the beta subunit and activated the...

Heme oxygenase-carbon monoxide signalling pathway in atherosclerosis: anti-atherogenic actions of bilirubin and carbon monoxide? (1999)

Siow, Richard C.M., Sato, Hideyo, Mann, Giovanni E.

Atherosclerosis is a major contributor to cardiovascular disease, and genetic disorders of lipoprotein metabolism are recognized risk factors in atherogenesis. The gaseous monoxides nitric oxide (NO)...

Mechanisms underlying β2-adrenoceptor-mediated nitric oxide generation by human umbilical vein endothelial cells

Queen, Lindsay R, Ji, Yong, Xu, Biao, Young, Lora, Yao, Kang, Wyatt, Amanda W, ...

Endothelial β2-adrenoceptor (β2AR) stimulation increases nitric oxide (NO) generation, but the underlying cellular mechanisms are unclear. We examined the role of l-arginine transport and of...

Mechanisms of acute vasodilator response to bacterial lipopolysaccharide in the rat coronary microcirculation

Cannon, Toby R, Mann, Giovanni E, Baydoun, Anwar R

In this study the mechanisms of the acute vasodilator action of bacterial lipopolysaccharide (LPS) were investigated in the rat Langendorff perfused heart.Infusion of LPS (5 μg ml−1) caused a...

Role of L-citrulline transport in nitric oxide synthesis in rat aortic smooth muscle cells activated with LPS and interferon-γ

Wileman, Samantha M, Mann, Giovanni E, Pearson, Jeremy D, Baydoun, Anwar R

L-citrulline, a coproduct of nitric oxide synthase (NOS)-catalysed metabolism of L-arginine to nitric oxide (NO), is an important intermediate of the urea cycle and a precursor for L-arginine...

Induction of cyclooxygenase-2 expression in human myometrial smooth muscle cells by interleukin-1β: involvement of p38 mitogen-activated protein kinase

Bartlett, Simon R, Sawdy, Robert, Mann, Giovanni E

Human myometrial smooth muscle cells (HMSMCs) in culture were exposed to recombinant human interleukin-1β (IL-1β, 10 ng ml−1) for 1 to 24 h. Cyclooxygenase-2 (COX-2) mRNA and protein were rapidly...

Expression of nitric oxide synthase isoforms in pregnant human myometrium

Bartlett, Simon R, Bennett, Phillip R, Campa, Juan S, Dennes, William J B, Slater, Donna M, Mann, Giovanni E, ...

Endogenous nitric oxide has been proposed to play a role in the control of myometrial contractility in pregnancy. In this study, the expression, localisation and regulation of nitric oxide synthase...

Regulation of adenosine transport by D-glucose in human fetal endothelial cells: involvement of nitric oxide, protein kinase C and mitogen-activated protein kinase

Montecinos, Viviana P, Aguayo, Claudio, Flores, Carlos, Wyatt, Amanda W, Pearson, Jeremy D, Mann, Giovanni E, ...

The effects of elevated D-glucose on adenosine transport were investigated in human cultured umbilical vein endothelial cells isolated from normal pregnancies.Elevated D-glucose resulted in a time-...

Modulation of adenosine transport by insulin in human umbilical artery smooth muscle cells from normal or gestational diabetic pregnancies

Aguayo, Claudio, Flores, Carlos, Parodi, Jorge, Rojas, Romina, Mann, Giovanni E, Pearson, Jeremy D, ...

Adenosine transport was measured in human cultured umbilical artery smooth muscle cells, isolated from non-diabetic or gestational diabetic pregnancies, under basal conditions and after pretreatment...

Endothelial dysfunction and reduced antioxidant protection in an animal model of the developmental origins of cardiovascular disease

Rodford, Joanne L, Torrens, Christopher, Siow, Richard C M, Mann, Giovanni E, Hanson, Mark A, Clough, Geraldine F

Endothelial dysfunction underlies cardiovascular disease (CVD) in humans and is reported in animal models of developmental origins of such disease. We have investigated whether impaired antioxidant...