Detection of ligand binding to nucleotide sugar transporters by STD NMR spectroscopy (2008)
Maggioni, Andrea, Von Itzstein, Mark, Tiralongo, Giuseppe, Haselhorst, Thomas Erwin
Yes
Detection of ligand binding to nucleotide sugar transporters by STD NMR spectroscopy (2008)
Maggioni, Andrea, Von Itzstein, Mark, Tiralongo, Giuseppe, Haselhorst, Thomas Erwin
Detection of ligand binding to nucleotide sugar transporters by STD NMR spectroscopy (2008)
Maggioni, Andrea, Von Itzstein, Mark, Tiralongo, Giuseppe, Haselhorst, Thomas Erwin
Day, Christopher, Tiralongo, Giuseppe, Hartnell, Regan David, Von Itzstein, Mark, Wilson, Jenny, Korolik, Victoria
No
Tiralongo, Giuseppe, Fujita, Akiko, Sato, Chihiro, Kitajima, Ken, Lehmann, Friederike, Oschiles, Melanie, ...
Yes
Day, Christopher, Tiralongo, Giuseppe, Hartnell, Regan David, Von Itzstein, Mark, Wilson, Jenny, Korolik, Victoria
Tiralongo, Giuseppe, Fujita, Akiko, Sato, Chihiro, Kitajima, Ken, Lehmann, Friederike, Oschiles, Melanie, ...
Haselhorst, Thomas Erwin, Munster-Kuhnel, Anja K., Oschlies, Melanie, Tiralongo, Giuseppe, Gerardy-Schahn, Rita, Von Itzstein, Mark
Yes
Haselhorst, Thomas Erwin, Munster-Kuhnel, Anja K., Oschlies, Melanie, Tiralongo, Giuseppe, Gerardy-Schahn, Rita, Von Itzstein, Mark
Day, Christopher, Tiralongo, Giuseppe, Hartnell, Regan David, Von Itzstein, Mark, Wilson, Jenny, Korolik, Victoria
Tiralongo, Giuseppe, Fujita, Akiko, Sato, Chihiro, Kitajima, Ken, Lehmann, Friederike, Oschiles, Melanie, ...
The terminal sugar sialic acid (Sia) plays a pivotal role in cell–cell interaction and recognition. A prerequisite for the biosynthesis of sialoglycoconjugates is the activation of Sia to cytidine...
Maggioni, Andrea, Von Itzstein, Mark, Gerardy-Schahn, Rita, Tiralongo, Giuseppe
The heterologous expression of functional mammalian integral membrane proteins still represents a significant hurdle towards their crystallization and structure elucidation. We have therefore...
Haselhorst, Thomas Erwin, Munster-Kuhnel, Anja K., Oschlies, Melanie, Tiralongo, Giuseppe, Gerardy-Schahn, Rita, Von Itzstein, Mark
We report an easy and direct application of 'Saturation Transfer Double Difference' (STDD) NMR spectroscopy to identify ligands that bind to a Sepharose-immobilised target protein. The model protein,...
Tiralongo, Giuseppe, Ashikov, Angel, Routier, Françoise, Eckhardt, Matthias, Bakker, Hans, Gerardy-Schahn, Rita, ...
Yes
Haselhorst, Thomas Erwin, Oschlies, Melanie, Abu-Izneid, Tareq, Kiefel, Milton, Tiralongo, Giuseppe, Münster-Kühnel, Anja K., ...
Yes
Tiralongo, Giuseppe, Ashikov, Angel, Routier, Françoise, Eckhardt, Matthias, Bakker, Hans, Gerardy-Schahn, Rita, ...
Yes
Haselhorst, Thomas Erwin, Oschlies, Melanie, Abu-Izneid, Tareq, Kiefel, Milton, Tiralongo, Giuseppe, Münster-Kühnel, Anja K., ...
Yes
Tiralongo, Giuseppe, Ashikov, Angel, Routier, Françoise, Eckhardt, Matthias, Bakker, Hans, Gerardy-Schahn, Rita, ...
Haselhorst, Thomas Erwin, Oschlies, Melanie, Abu-Izneid, Tareq, Kiefel, Milton, Tiralongo, Giuseppe, Münster-Kühnel, Anja K., ...
Tiralongo, Giuseppe, Ashikov, Angel, Routier, Françoise, Eckhardt, Matthias, Bakker, Hans, Gerardy-Schahn, Rita, ...
The architectural conservation of nucleotide sugar transport proteins (NSTs) enabled the theoretical prediction of putative NSTs in diverse gene databases. In the human genome, 17 NST sequences have...
Haselhorst, Thomas Erwin, Oschlies, Melanie, Abu-Izneid, Tareq, Kiefel, Milton, Tiralongo, Giuseppe, Münster-Kühnel, Anja K., ...
CMP-Kdn synthetase catalyses the reaction of sialic acids (Sia) and CTP to the corresponding activated sugar nucleotide CMP-Sia and pyrophosphate PP i . Saturation Transfer Difference (STD) NMR...
Probing a CMP-Kdn synthetase by 1H, 31P, and STD NMR spectroscopy. (2005)
Haselhorst, Thomas Erwin, Muenster-Kuehnel, Anja K., Stolz, Anita, Oschiles, Melanie, Tiralongo, Giuseppe, Kitajima, Ken, ...
Yes
Functional expression of the CMP-sialic acid transporter in Escherichia coli (2005)
Tiralongo, Giuseppe, Maggioni, Andrea, Gerardy-Schahn, Rita, Von Itzstein, Mark
No
Probing a CMP-Kdn synthetase by 1H, 31P, and STD NMR spectroscopy. (2005)
Haselhorst, Thomas Erwin, Muenster-Kuehnel, Anja K., Stolz, Anita, Oschiles, Melanie, Tiralongo, Giuseppe, Kitajima, Ken, ...
Yes
Functional expression of the CMP-sialic acid transporter in Escherichia coli (2005)
Tiralongo, Giuseppe, Maggioni, Andrea, Gerardy-Schahn, Rita, Von Itzstein, Mark
No
Probing a CMP-Kdn synthetase by 1H, 31P, and STD NMR spectroscopy. (2005)
Haselhorst, Thomas Erwin, Muenster-Kuehnel, Anja K., Stolz, Anita, Oschiles, Melanie, Tiralongo, Giuseppe, Kitajima, Ken, ...
Functional expression of the CMP-sialic acid transporter in Escherichia coli (2005)
Tiralongo, Giuseppe, Maggioni, Andrea, Gerardy-Schahn, Rita, Von Itzstein, Mark
Probing a CMP-Kdn synthetase by 1H, 31P, and STD NMR spectroscopy. (2005)
Haselhorst, Thomas Erwin, Muenster-Kuehnel, Anja K., Stolz, Anita, Oschiles, Melanie, Tiralongo, Giuseppe, Kitajima, Ken, ...
CMP-Kdn synthetase catalyses the reaction of sialic acids (Sia) and cytidine-5′-triphosphate (CTP) to the corresponding activated sugar nucleotide CMP-Sia and pyrophosphate PPi. STD NMR experiments...
Functional expression of the CMP-sialic acid transporter in Escherichia coli (2005)
Tiralongo, Giuseppe, Maggioni, Andrea, Gerardy-Schahn, Rita, Von Itzstein, Mark
Structure and function of vertebrate CMP-sialic acid synthetase (2004)
Muenster-Kuehnel, Anja K., Tiralongo, Giuseppe, Krapp, Stephan, Weinhold, Birgit, Ritz-Sedlacek, Valentina, Jacob, Uwe, ...
Activation of sugars into nucleotide sugars is critical for their entry into biosynthetic pathways. In eukaryotic cells, the activation of the acidic nine-carbon sugar sialic acid to CMP sialic acid...
The Enigma of Enzymatic Sialic Acid O-Acetylation (2004)
Tiralongo, Giuseppe, Schauer, Roland, Ken-ichi Kasai (Kanagawa)
Yes
Structure and function of vertebrate CMP-sialic acid synthetase (2004)
Muenster-Kuehnel, Anja K., Tiralongo, Giuseppe, Krapp, Stephan, Weinhold, Birgit, Ritz-Sedlacek, Valentina, Jacob, Uwe, ...
Activation of sugars into nucleotide sugars is critical for their entry into biosynthetic pathways. In eukaryotic cells, the activation of the acidic nine-carbon sugar sialic acid to CMP sialic acid...
O-Acetylation and de-O-acetylation of sialic acids in human colorectal carcinoma (2004)
Shen, Yanqin, Kohla, Guido, Lrhorfi, Aichia L., Sipos, Bence, Kalthoff, Holger, Gerwig, Gerrit J., ...
Adecrease in the level ofO-acetylated sialic acids observed in colorectal carcinoma may lead to an increase in the expression of sialyl LewisX, a tumor-associated antigen, which is related to...
O-Acetylation and de-O-acetylation of sialic acids in human colorectal carcinoma (2004)
Shen, Yanqin, Kohla, Guido, Lrhorfi, Aichia L., Sipos, Bence, Kalthoff, Holger, Gerwig, Gerrit J., ...
Adecrease in the level ofO-acetylated sialic acids observed in colorectal carcinoma may lead to an increase in the expression of sialyl LewisX, a tumor-associated antigen, which is related to...
Structure and function of vertebrate CMP-sialic acid synthetase (2004)
Muenster-Kuehnel, Anja K., Tiralongo, Giuseppe, Krapp, Stephan, Weinhold, Birgit, Ritz-Sedlacek, Valentina, Jacob, Uwe, ...
Activation of sugars into nucleotide sugars is critical for their entry into biosynthetic pathways. In eukaryotic cells, the activation of the acidic nine-carbon sugar sialic acid to CMP sialic acid...
O-Acetylation and de-O-acetylation of sialic acids in human colorectal carcinoma (2004)
Shen, Yanqin, Kohla, Guido, Lrhorfi, Aichia L., Sipos, Bence, Kalthoff, Holger, Gerwig, Gerrit J., ...
Adecrease in the level ofO-acetylated sialic acids observed in colorectal carcinoma may lead to an increase in the expression of sialyl LewisX, a tumor-associated antigen, which is related to...
Regulation of sialic acid O-acetylation in human colon mucosa (2004)
Shen, Yanqin, Tiralongo, Giuseppe, Kohla, Guido, Schauer, Roland
The expression of O-acetylated sialic acids in human colonic mucins is developmentally regulated, and a reduction of O-acetylation has been found to be associated with the early stages of colorectal...
Regulation of sialic acid O-acetylation in human colon mucosa (2004)
Shen, Yanqin, Tiralongo, Giuseppe, Kohla, Guido, Schauer, Roland
The expression of O-acetylated sialic acids in human colonic mucins is developmentally regulated, and a reduction of O-acetylation has been found to be associated with the early stages of colorectal...
Structure and function of vertebrate CMP-sialic acid synthetase (2004)
Muenster-Kuehnel, Anja K., Tiralongo, Giuseppe, Krapp, Stephan, Weinhold, Birgit, Ritz-Sedlacek, Valentina, Jacob, Uwe, ...
Activation of sugars into nucleotide sugars is critical for their entry into biosynthetic pathways. In eukaryotic cells, the activation of the acidic nine-carbon sugar sialic acid to CMP–sialic...
The Enigma of Enzymatic Sialic Acid O-Acetylation (2004)
Tiralongo, Giuseppe, Schauer, Roland
Naturally occurring sialic acids can be O-acetylated at any one of four hydroxyl groups, located at position C-4, -7, -8, and -9. This modification, which is found in nearly all animals expressing...
O-Acetylation and de-O-acetylation of sialic acids in human colorectal carcinoma (2004)
Shen, Yanqin, Kohla, Guido, Lrhorfi, Aichia L., Sipos, Bence, Kalthoff, Holger, Gerwig, Gerrit J., ...
Adecrease in the level ofO-acetylated sialic acids observed in colorectal carcinoma may lead to an increase in the expression of sialyl LewisX, a tumor-associated antigen, which is related to...
Regulation of sialic acid O-acetylation in human colon mucosa (2004)
Shen, Yanqin, Tiralongo, Giuseppe, Kohla, Guido, Schauer, Roland
The expression of O-acetylated sialic acids in human colonic mucins is developmentally regulated, and a reduction of O-acetylation has been found to be associated with the early stages of colorectal...
The crystal structure of murine CMP-5-N-acetylneuraminic acid synthetase. (2003)
Krapp, Stephan, Münster-Kühnel, Anja K., Kaiser, Jens, Huber, Robert, Tiralongo, Giuseppe, Gerardy-Schahn, Rita, ...
Sialic acids are activated by CMP-5-N-acetylneuraminic acid synthetase prior to their transfer onto oligo- or polysaccharides. Here, we present the crystal structure of the N-terminal catalytically...
The crystal structure of murine CMP-5-N-acetylneuraminic acid synthetase. (2003)
Krapp, Stephan, Münster-Kühnel, Anja K., Kaiser, Jens, Huber, Robert, Tiralongo, Giuseppe, Gerardy-Schahn, Rita, ...
Sialic acids are activated by CMP-5-N-acetylneuraminic acid synthetase prior to their transfer onto oligo- or polysaccharides. Here, we present the crystal structure of the N-terminal catalytically...
The crystal structure of murine CMP-5-N-acetylneuraminic acid synthetase. (2003)
Krapp, Stephan, Münster-Kühnel, Anja K., Kaiser, Jens, Huber, Robert, Tiralongo, Giuseppe, Gerardy-Schahn, Rita, ...
Sialic acids are activated by CMP-5-N-acetylneuraminic acid synthetase prior to their transfer onto oligo- or polysaccharides. Here, we present the crystal structure of the N-terminal catalytically...
Shen, Yanqin, Tiralongo, Giuseppe, Iwersen, Matthias, Sipos, Bence, Kalthoff, Holger, Schauer, Roland
Sialic acids present on human colonic mucins are highly O-acetylated, however, little is known about the underlying enzymatic activity required for O-acetylation in this tissue. Here we report on the...
Shen, Yanqin, Tiralongo, Giuseppe, Iwersen, Matthias, Sipos, Bence, Kalthoff, Holger, Schauer, Roland
Sialic acids present on human colonic mucins are highly O-acetylated, however, little is known about the underlying enzymatic activity required for O-acetylation in this tissue. Here we report on the...
Shen, Yanqin, Tiralongo, Giuseppe, Iwersen, Matthias, Sipos, Bence, Kalthoff, Holger, Schauer, Roland
Sialic acids present on human colonic mucins are highly O-acetylated, however, little is known about the underlying enzymatic activity required for O-acetylation in this tissue. Here we report on the...
Shen, Yanqin, Tiralongo, Giuseppe, Iwersen, Matthias, Sipos, Bence, Kalthoff, Holger, Schauer, Roland
Sialic acids present on human colonic mucins are highly O-acetylated, however, little is known about the underlying enzymatic activity required for O-acetylation in this tissue. Here we report on the...