Bärbel Stecher, Riccardo Robbiani, Alan W. Walker, Astrid M. Westendorf, Manja Barthel, Marcus Kremer, ...
Most mucosal surfaces of the mammalian body are colonized by microbial communities (“microbiota”). A high density of commensal microbiota inhabits the intestine and shields from infection...
A Novel Linear Plasmid Mediates Flagellar Variation in Salmonella Typhi (2007)
Stephen Baker, Jonathan Hardy, Kenneth E. Sanderson, Michael Quail, Ian Goodhead, Robert A. Kingsley, ...
Unlike the majority of Salmonella enterica serovars, Salmonella Typhi (S. Typhi), the etiological agent of human typhoid, is monophasic. S. Typhi normally harbours only the phase 1 flagellin gene...
Nicholas R. Thomson, Sarah Howard, Brendan W. Wren, Lisa Crossman, Gregory L. Challis, ...
The human enteropathogen, Yersinia enterocolitica, is a significant link in the range of Yersinia pathologies extending from mild gastroenteritis to bubonic plague. Comparison at the genomic level is...
Evolutionary history of Salmonella Typhi (2006)
Roumagnac, Philippe, Weill, François-Xavier, Dolecek, Christiane, Baker, Stephen, Brisse, Sylvain, Chinh, Nguyen Tran, ...
Li, Chris K.F., Pender, Sylvia L.F., Pickard, Karen M., Chance, Victoria, Holloway, Judith A., Huett, Alan, ...
Infection of mice with the intestinal bacterial pathogen Citrobacter rodentium results in colonic mucosal hyperplasia and a local Th1 inflammatory response similar to that seen in mouse models of...
Wain, John, Hien, Tran T., Connerton, Phillippa, Ali, Tahir, M. Parry, Christopher, Chinh, Nguyen T. T., ...
The rate of multiple-antibiotic resistance is increasing among Salmonella enterica serovar Typhi strains in Southeast Asia. Pulsed-field gel electrophoresis (PFGE) and other typing methods were used...
Development of a Universal Intimin Antiserum and PCR Primers
Batchelor, Miranda, Knutton, Stuart, Caprioli, Alfredo, Huter, Veronika, Zanial, Mazlina, Dougan, Gordon, ...
Enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic E. coli (EHEC) constitute a significant risk to human health worldwide. A hallmark of both pathogens is their ability to produce...
Connerton, Phillippa, Wain, John, Hien, Tran T., Ali, Tahir, Parry, Christopher, Chinh, Nguyen T., ...
Multidrug-resistant Salmonella enterica serotype Typhi isolates from four outbreaks of typhoid fever in southern Vietnam between 1993 and 1997 were compared. Pulsed-field gel electrophoresis,...
Serology of Typhoid Fever in an Area of Endemicity and Its Relevance to Diagnosis
House, Deborah, Wain, John, Ho, Vo A., Diep, To S., Chinh, Nguyen T., Bay, Phan V., ...
Currently, the laboratory diagnosis of typhoid fever is dependent upon either the isolation of Salmonella enterica subsp. enterica serotype Typhi from a clinical sample or the detection of raised...
Ferrioxamine-Mediated Iron(III) Utilization by Salmonella enterica
Kingsley, Robert A., Reissbrodt, Rolf, Rabsch, Wolfgang, Ketley, Julian M., Tsolis, Renée M., Everest, Paul, ...
Utilization of ferrioxamines as sole sources of iron distinguishes Salmonella enterica serotypes Typhimurium and Enteritidis from a number of related species, including Escherichia coli. Ferrioxamine...
Prentice, Michael B., James, Keith D., Parkhill, Julian, Baker, Stephen G., Stevens, Kim, Simmonds, Mark N., ...
Population genetic studies suggest that Yersinia pestis, the cause of plague, is a clonal pathogen that has recently emerged from Yersinia pseudotuberculosis. Plasmid acquisition is likely to have...
Lowe, David C., Savidge, Tor C., Pickard, Derek, Eckmann, Lars, Kagnoff, Martin F., Dougan, Gordon, ...
The properties of two candidate Salmonella typhi-based live oral typhoid vaccine strains, BRD691 (S. typhi Ty2 harboring mutations in aroA and aroC) and BRD1116 (S. typhi Ty2 harboring mutations in...
Evaluation of Salmonella typhimurium Mutants in a Model of Experimental Gastroenteritis
Everest, Paul, Ketley, Julian, Hardy, Simon, Douce, Gill, Khan, Shahid, Shea, Jacqui, ...
Salmonella typhimurium strains harboring independent, defined mutations in aroA, invA, ssrA, or msbB were assessed for their ability to induce fluid accumulation, tissue damage, and local...
Higgins, Lisa M., Frankel, Gad, Douce, Gill, Dougan, Gordon, MacDonald, Thomas T.
Citrobacter rodentium is a classically noninvasive pathogen of mice that is similar to enteropathogenic Escherichia coli (EPEC) in man. Following oral infection of young mice, the organism colonizes...
Douce, Gill, Giannelli, Valentina, Pizza, Mariagrazia, Lewis, David, Everest, Paul, Rappuoli, Rino, ...
Detoxified mutants of the Escherichia coli heat-labile toxin (LT) act as mucosal adjuvants to intranasally presented coadministered antigens. Here, we compare the adjuvant activity of a panel of...
Ward, Stephen J., Douce, Gill, Dougan, Gordon, Wren, Brendan W.
Fourteen of the 38 C-terminal repeats from Clostridium difficile toxin A (14CDTA) were cloned and expressed either with an N-terminal polyhistidine tag (14CDTA-HIS) or fused to the nontoxic binding...
Salmonella enterica Serovar Typhimurium surA Mutants Are Attenuated and Effective Live Oral Vaccines
Sydenham, Mark, Douce, Gillian, Bowe, Frances, Ahmed, Saddif, Chatfield, Steve, Dougan, Gordon
A previously described attenuated TnphoA mutant (BRD441) of Salmonella enterica serovar Typhimurium C5 (I. Miller, D. Maskell, C. Hormaeche, K. Johnson, D. Pickard, and G. Dougan, Infect. Immun....
Tacket, Carol O., Sztein, Marcelo B., Wasserman, Steven S., Losonsky, Genevieve, Kotloff, Karen L., Wyant, Timothy L., ...
Salmonella enterica serovar Typhi strain CVD 908-htrA is a live attenuated strain which may be useful as an improved oral typhoid vaccine and as a vector for cloned genes of other pathogens. We...
Inhibition of T-cell Response by Escherichia coli Heat-Labile Enterotoxin-Treated Epithelial Cells
Lopes, Luciene M., Maroof, Asher, Dougan, Gordon, Chain, Benjamin M.
Escherichia coli heat-labile enterotoxin (LT) is an extensively studied adjuvant of mucosal responses. Nevertheless, its mode of action as an adjuvant remains incompletely understood. In this study,...
Salmonella enterica Serovar Typhi Possesses a Unique Repertoire of Fimbrial Gene Sequences
Townsend, Stacy M., Kramer, Naomi E., Edwards, Robert, Baker, Stephen, Hamlin, Nancy, Simmonds, Mark, ...
Salmonella enterica serotype Typhi differs from nontyphoidal Salmonella serotypes by its strict host adaptation to humans and higher primates. Since fimbriae have been implicated in host adaptation,...
Expression of Intimin γ from Enterohemorrhagic Escherichia coli in Citrobacter rodentium
Hartland, Elizabeth L., Huter, Veronika, Higgins, Lisa M., Goncalves, Nathalie S., Dougan, Gordon, Phillips, Alan D., ...
The carboxy-terminal 280 amino acids (Int280) of the bacterial adhesion molecule intimin include the receptor-binding domain. At least five different types of Int280, designated α, β, γ, δ, and...
Ghaem-Maghami, Marjan, Simmons, Cameron P., Daniell, Sarah, Pizza, Mariagrazia, Lewis, David, Frankel, Gad, ...
The formation of attaching and effacing (A/E) lesions on gut enterocytes is central to the pathogenesis of enterohemorrhagic (EHEC) Escherichia coli, enteropathogenic E. coli (EPEC), and the rodent...
Gonçalves, Nathalie S., Ghaem-Maghami, Marjan, Monteleone, Giovanni, Frankel, Gad, Dougan, Gordon, Lewis, David J. M., ...
Infection of mice with the intestinal bacterial pathogen Citrobacter rodentium results in colonic mucosal hyperplasia and a local Th1 inflammatory response similar to that seen in mouse models of...
Adu-Bobie, Jeannette, Frankel, Gad, Bain, Christopher, Goncalves, Azizedite Guedes, Trabulsi, Luiz R., Douce, Gill, ...
Intimins are outer membrane proteins expressed by enteric bacterial pathogens capable of inducing intestinal attachment-and-effacement lesions. A eukaryotic cell-binding domain is located within a...
Donnenberg, Michael S., Tacket, Carol O., Losonsky, Genevieve, Frankel, Gad, Nataro, James P., Dougan, Gordon, ...
Two studies of adult volunteers were performed to determine whether prior enteropathogenic Escherichia coli (EPEC) infection confers protective immunity against rechallenge. In the first study, a...
Hicks, Susan, Frankel, Gad, Kaper, James B., Dougan, Gordon, Phillips, Alan D.
Attaching and effacing (A/E) lesion formation is central to enteropathogenic Escherichia coli (EPEC) pathogenesis. In vitro experiments with human epithelial cell lines have implicated virulence...
Everest, Paul, Roberts, Mark, Dougan, Gordon
Mice defective in the ability to produce the tumor necrosis factor alpha p55 receptor (TNFαp55R) were orally challenged with a number of Salmonella typhimurium HWSH derivatives that differ in...
Adu-Bobie, Jeannette, Trabulsi, Luiz R., Dougan, Gordon, Frankel, Gad
Enteropathogenic Escherichia coli (EPEC) strains are a common cause of infantile diarrhea in developing countries. EPEC strains induce a characteristic attaching and effacing (A/E) lesion on...
McClelland, Michael, Florea, Liliana, Sanderson, Ken, Clifton, Sandra W., Parkhill, Julian, Churcher, Carol, ...
The Escherichia coli K-12 genome (ECO) was compared with the sampled genomes of the sibling species Salmonella enterica serovars Typhimurium, Typhi and Paratyphi A (collectively referred to as SAL)...
Ward, Stephen J., Douce, Gill, Figueiredo, Dayse, Dougan, Gordon, Wren, Brendan W.
The C-terminal repeat domain of Clostridium difficile toxin A harbors toxin-neutralizing epitopes and is considered to be a candidate component of a vaccine against C. difficile-associated disease...
Increased Susceptibility of C1q-Deficient Mice to Salmonella enterica Serovar Typhimurium Infection
Warren, Joanna, Mastroeni, Pietro, Dougan, Gordon, Noursadeghi, Mahdad, Cohen, Jonathan, Walport, Mark J., ...
The role of the complement system in host defense against Salmonella infection is poorly defined. Bacterial cell wall O-antigen polysaccharide can activate the alternative pathway in vitro. No...
Hindle, Zoë, Chatfield, Steven N., Phillimore, Jo, Bentley, Matthew, Johnson, Julie, Cosgrove, Catherine A., ...
The attenuation and immunogenicity of two novel Salmonella vaccine strains, Salmonella enterica serovar Typhi (Ty2 ΔaroC ΔssaV, designated ZH9) and S. enterica serovar Typhimurium (TML ΔaroC...
Chan, Kaman, Baker, Stephen, Kim, Charles C., Detweiler, Corrella S., Dougan, Gordon, Falkow, Stanley
The genus Salmonella consists of over 2,200 serovars that differ in their host range and ability to cause disease despite their close genetic relatedness. The genetic factors that influence each...
Kingsley, Robert A., Humphries, Andrea D., Weening, Eric H., De Zoete, Marcel R., Winter, Sebastian, Papaconstantinopoulou, Anastasia, ...
The shdA gene is carried on a 25-kb genetic island at centisome 54 (CS54 island) of the Salmonella enterica serotype Typhimurium chromosome. In addition to shdA, the CS54 island of Salmonella...
Expression of tetanus toxin Fragment C in tobacco chloroplasts
Tregoning, John S., Nixon, Peter, Kuroda, Hiroshi, Svab, Zora, Clare, Simon, Bowe, Frances, ...
Fragment C (TetC) is a non-toxic 47 kDa polypeptide fragment of tetanus toxin that can be used as a subunit vaccine against tetanus. Expression of TetC in Escherichia coli and yeast was dependent on...
Neves, Bianca C., Mundy, Rosanna, Petrovska, Liljana, Dougan, Gordon, Knutton, Stuart, Frankel, Gad
Enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic E. coli are extracellular pathogens that employ a type III secretion system to export translocator and effector proteins, proteins which...
Gonçalves, Nathalie S., Hale, Christine, Dougan, Gordon, Frankel, Gad, MacDonald, Thomas T.
Intimin-conjugated fluorescent beads bind to spleen CD4 T cells and Peyer's patch, mesenteric lymph node, and cecal follicle lymphocytes, with less binding to lamina propria T cells and...
Jenkins, Claire, Perry, Neil T., Cheasty, Tom, Shaw, Darren J., Frankel, Gad, Dougan, Gordon, ...
Certain strains of Shiga toxin-producing Escherichia coli (STEC) which do not have the locus of enterocyte effacement pathogenicity island carry the STEC autoagglutinating adhesin (saa) gene. The...
Pickard, Derek, Wain, John, Baker, Stephen, Line, Alexandra, Chohan, Sonia, Fookes, Maria, ...
Vi capsular polysaccharide production is encoded by the viaB locus, which has a limited distribution in Salmonella enterica serovars. In S. enterica serovar Typhi, viaB is encoded on a 134-kb...
Wain, John, Diem Nga, L. T., Kidgell, Claire, James, Keith, Fortune, Sarah, Song Diep, To, ...
The first outbreak of multidrug-resistant (MDR) typhoid fever in Vietnam was in 1993, and by 1995 nearly 90% of cases were MDR. Plasmid HCM1, sequenced in full, is an incHI1 plasmid from Salmonella...
Simmons, Cameron P., Clare, Simon, Ghaem-Maghami, Marjan, Uren, Tania K., Rankin, Joanna, Huett, Allan, ...
Citrobacter rodentium, an attaching-effacing bacterial pathogen, establishes an acute infection of the murine colonic epithelium and induces a mild colitis in immunocompetent mice. This study...
Intracellular Adhesion Molecule 1 Plays a Key Role in Acquired Immunity to Salmonellosis
Clare, Simon, Goldin, Robert, Hale, Christine, Aspinall, Richard, Simmons, Cameron, Mastroeni, Pietro, ...
This study investigated the role of intracellular adhesion molecule 1 (ICAM-1) during Salmonella enterica serovar Typhimurium infection of mice. We show that ICAM-1 is expressed in and around...
Batchelor, Miranda, Prasannan, Sunil, Daniell, Sarah, Reece, Stephen, Connerton, Ian, Bloomberg, Graham, ...
Intimin is a bacterial adhesion molecule involved in intimate attachment of enteropathogenic and enterohaemorrhagic Escherichia coli to mammalian host cells. Intimin targets the translocated intimin...
Mundy, Rosanna, Petrovska, Liljana, Smollett, Katherine, Simpson, Nandi, Wilson, Rebecca K., Yu, Jun, ...
Citrobacter rodentium is a member of a group of pathogens that colonize the lumen of the host gastrointestinal tract via attaching and effacing (A/E) lesion formation. C. rodentium, which causes...
Bowe, Frances, Lavelle, Ed C., McNeela, Edel A., Hale, Christine, Clare, Simon, Arico, Beatrice, ...
Conjugated polysaccharide vaccines protect against serogroup C meningococci. However, this approach cannot be applied to serogroup B, which is still a major cause of meningitis. We evaluated the...
Transcriptome of Enterohemorrhagic Escherichia coli O157 Adhering to Eukaryotic Plasma Membranes
Dahan, Sivan, Knutton, Stuart, Shaw, Robert K., Crepin, Valerie F., Dougan, Gordon, Frankel, Gad
Using a DNA microarray, we determined changes in enterohemorrhagic Escherichia coli O157:H7 gene expression during binding to plasma membranes. Analysis of the complete transcriptomes of the bound...
Stratford, Richard, McKelvie, Nicola D., Hughes, Nicky J., Aldred, Emma, Wiseman, Claire, Curtis, Johanna, ...
Novel candidate live oral vaccines based on a Salmonella enterica serovar Typhi ZH9 (Ty2 ΔaroC ΔssaV) derivative that directed the expression of either the B subunit of Escherichia coli heat-labile...
Analysis of the Hypervariable Region of the Salmonella enterica Genome Associated with tRNAleuX†
Bishop, Anne L., Baker, Stephen, Jenks, Sara, Fookes, Maria, Gaora, Peadar Ó, Pickard, Derek, ...
The divergence of Salmonella enterica and Escherichia coli is estimated to have occurred approximately 140 million years ago. Despite this evolutionary distance, the genomes of these two species...
Vi Antigen Expression in Salmonella enterica Serovar Typhi Clinical Isolates from Pakistan
Wain, John, House, Deborah, Zafar, Afia, Baker, Stephen, Nair, Satheesh, Kidgell, Claire, ...
The accurate identification of Salmonella enterica subsp. enterica serovar Typhi variants that fail to express the capsular polysaccharide, Vi, is an important and much discussed issue for medical...
Jomaa, Maha, Yuste, Jose, Paton, James C., Jones, Christopher, Dougan, Gordon, Brown, Jeremy S.
PiaA and PiuA are the lipoprotein components of the Pia and Piu Streptococcus pneumoniae iron uptake ABC transporters and are required for full virulence in mouse models of infection. Active or...
Use of Paired Serum Samples for Serodiagnosis of Typhoid Fever
House, Deborah, Chinh, Nguyen T., Diep, To S., Parry, Christopher M., Wain, John, Dougan, Gordon, ...
Using an enzyme-linked immunosorbent assay we demonstrate that, in adult patients with typhoid fever, the sensitivity of a serological test based on the detection of anti-lipopolysaccharide...
Baker, Stephen, Sarwar, Yasra, Aziz, Hafsa, Haque, Asma, Ali, Aamir, Dougan, Gordon, ...
The synthesis and transportation proteins of the Vi capsular polysaccharide of Salmonella enterica serovar Typhi (serovar Typhi) are encoded by the viaB operon, which resides on a 134-kb...
The lrp Gene and Its Role in Type I Fimbriation in Citrobacter rodentium
Cordone, Angela, Mauriello, Emilia M. F., Pickard, Derek J., Dougan, Gordon, De Felice, Maurilio, Ricca, Ezio
Citrobacter rodentium is a murine pathogen that is now widely used as an in vivo model for gastrointestinal infections due to its similarities with human enteropathogens, such as the possession of a...
Anjum, Muna F., Marooney, Chris, Fookes, Maria, Baker, Stephen, Dougan, Gordon, Ivens, Al, ...
We have performed microarray hybridization studies on 40 clinical isolates from 12 common serovars within Salmonella enterica subspecies I to identify the conserved chromosomal gene pool. We were...
Cosgrove, Catherine A., Hussell, Tracy, Sexton, Amy, Giemza, Rafaela, Phillips, Richard, ...
Oral immunization of healthy adults with 107 CFU BCG Moreau Rio de Janeiro was well tolerated and significantly boosted gamma interferon responses to purified protein derivative, Ag85, and MPB70 from...
Wain, John, Hien, Tran T., Connerton, Phillippa, Ali, Tahir, M. Parry, Christopher, Chinh, Nguyen T. T., ...
The rate of multiple-antibiotic resistance is increasing among Salmonella enterica serovar Typhi strains in Southeast Asia. Pulsed-field gel electrophoresis (PFGE) and other typing methods were used...
Development of a Universal Intimin Antiserum and PCR Primers
Batchelor, Miranda, Knutton, Stuart, Caprioli, Alfredo, Huter, Veronika, Zanial, Mazlina, Dougan, Gordon, ...
Enteropathogenic Escherichia coli (EPEC) and entero