Charged Amino Acids in a Preprotein Inhibit SecA-Dependent Protein Translocation (2009)
Nouwen, Nico, Berrelkamp, Greetje, Driessen, Arnold J.M.
Sec translocase catalyzes membrane protein insertion and translocation. We have introduced stretches of charged amino acid residues into the preprotein proOmpA and have analyzed their effect on in...
The Lateral Gate of SecYEG Opens during Protein Translocation (2009)
Berrelkamp, Greetje, Nouwen, Nico, Driessen, Arnold J.M.
The SecYEG translocon of Escherichia coli mediates the translocation of preproteins across the cytoplasmic membrane. Here, we have examined the role of the proposed lateral gate of the translocon in...
Bacterial Sec-translocase Unfolds and Translocates a Class of Folded Protein Domains (2007)
Nouwen, Nico, Berrelkamp, Greetje, Driessen, Arnold J.M.
It is generally assumed that preprotein substrates must be presented in an unfolded state to the bacterial Sec-translocase in order to be translocated. Here, we have examined the ability of the...
Topologically Fixed SecG Is Fully Functional (2006)
Vries, Erhard Van Der, Berrelkamp, Greetje, Nouwen, Nico, Driessen, Arnold J.M.
It has been proposed that the bitopic membrane protein SecG undergoes topology inversion during translocation of (pre)proteins via SecYEG. Here we show that SecG covalently cross-linked to SecY...
Nouwen, Nico, Piwowarek, Magdalena, Berrelkamp, Greetje, Driessen, Arnold J.M.
A remarkable feature of proteins of the SecD and SecF family involved in protein translocation is that they possess a very large first periplasmic domain. Here we report that this large first...
The Large First Periplasmic Loop of SecD and SecF Plays an Important Role in SecDF Functioning
Nouwen, Nico, Piwowarek, Magdalena, Berrelkamp, Greetje, Driessen, Arnold J. M.
A remarkable feature of proteins of the SecD and SecF family involved in protein translocation is that they possess a very large first periplasmic domain. Here we report that this large first...
Topologically Fixed SecG Is Fully Functional
Van Der Sluis, Eli O., Van Der Vries, Erhard, Berrelkamp, Greetje, Nouwen, Nico, Driessen, Arnold J. M.
It has been proposed that the bitopic membrane protein SecG undergoes topology inversion during translocation of (pre)proteins via SecYEG. Here we show that SecG covalently cross-linked to SecY...
The Large First Periplasmic Loop of SecD and SecF Plays an Important Role in SecDF Functioning
Nouwen, Nico, Piwowarek, Magdalena, Berrelkamp, Greetje, Driessen, Arnold J. M.
A remarkable feature of proteins of the SecD and SecF family involved in protein translocation is that they possess a very large first periplasmic domain. Here we report that this large first...
Topologically Fixed SecG Is Fully Functional
Van Der Sluis, Eli O., Van Der Vries, Erhard, Berrelkamp, Greetje, Nouwen, Nico, Driessen, Arnold J. M.
It has been proposed that the bitopic membrane protein SecG undergoes topology inversion during translocation of (pre)proteins via SecYEG. Here we show that SecG covalently cross-linked to SecY...