Structural and functional studies of Oxytricha telomere-binding protein /--by Guowei Fang. (1993)
Thesis (Ph. D.)--University of Colorado, 1993.
Thesis (Ph. D.)--Michigan State University. Dept. of Horticulture, 1992.
Thesis (Ph. D.)--Michigan State University. Dept. of Horticulture, 1992.
Checkpoint Protein BubR1 Acts Synergistically with Mad2 to Inhibit Anaphase-promoting Complex
The spindle assembly checkpoint monitors the attachment of kinetochores to the mitotic spindle and the tension exerted on kinetochores by microtubules and delays the onset of anaphase until all the...
Fang, Guowei, Yu, Hongtao, Kirschner, Marc W.
The spindle assembly checkpoint mechanism delays anaphase initiation until all chromosomes are aligned at the metaphase plate. Activation of the anaphase-promoting complex (APC) by binding of CDC20...
MgcRacGAP controls the assembly of the contractile ring and the initiation of cytokinesis
Initiation of cytokinesis requires the establishment of the cleavage plane, the assembly of the contractile ring, and the ingression of the cleavage furrow. MgcRacGAP, a GTPase-activating protein for...
Anaphase-Promoting Complex/Cyclosome Controls the Stability of TPX2 during Mitotic Exit
TPX2, a microtubule-associated protein, is required downstream of Ran-GTP to induce spindle assembly. TPX2 activity appears to be tightly regulated during the cell cycle, and we report here one...
MAD2B is an inhibitor of the anaphase-promoting complex
Anaphase-promoting complex (APC), a ubiquitin ligase, controls both sister chromatid separation and mitotic exit. The APC is activated in mitosis and G1 by CDC20 and CDH1, and inhibited by the...
Zhao, Wei-meng, Seki, Akiko, Fang, Guowei
We report here an efficient functional genomic analysis by combining information on the gene expression profiling, cellular localization, and loss-of-function studies. Through this analysis, we...
Accurate chromosome segregation is controlled by the spindle checkpoint, which responds to the lack of microtubule–kinetochore attachment or of tension across sister kinetochores through...
Checkpoint Protein BubR1 Acts Synergistically with Mad2 to Inhibit Anaphase-promoting Complex
The spindle assembly checkpoint monitors the attachment of kinetochores to the mitotic spindle and the tension exerted on kinetochores by microtubules and delays the onset of anaphase until all the...
MAD2B is an inhibitor of the anaphase-promoting complex
Anaphase-promoting complex (APC), a ubiquitin ligase, controls both sister chromatid separation and mitotic exit. The APC is activated in mitosis and G1 by CDC20 and CDH1, and inhibited by the...
Fang, Guowei, Yu, Hongtao, Kirschner, Marc W.
The spindle assembly checkpoint mechanism delays anaphase initiation until all chromosomes are aligned at the metaphase plate. Activation of the anaphase-promoting complex (APC) by binding of CDC20...
MgcRacGAP controls the assembly of the contractile ring and the initiation of cytokinesis
Initiation of cytokinesis requires the establishment of the cleavage plane, the assembly of the contractile ring, and the ingression of the cleavage furrow. MgcRacGAP, a GTPase-activating protein for...
Anaphase-Promoting Complex/Cyclosome Controls the Stability of TPX2 during Mitotic Exit
TPX2, a microtubule-associated protein, is required downstream of Ran-GTP to induce spindle assembly. TPX2 activity appears to be tightly regulated during the cell cycle, and we report here one...
Zhao, Wei-meng, Seki, Akiko, Fang, Guowei
We report here an efficient functional genomic analysis by combining information on the gene expression profiling, cellular localization, and loss-of-function studies. Through this analysis, we...
Accurate chromosome segregation is controlled by the spindle checkpoint, which responds to the lack of microtubule–kinetochore attachment or of tension across sister kinetochores through...
Through a functional genomic screen for mitotic regulators, we identified hepatoma up-regulated protein (HURP) as a protein that is required for chromosome congression and alignment. In HURP-depleted...
Plx1 is the 3F3/2 kinase responsible for targeting spindle checkpoint proteins to kinetochores
Dynamic attachment of microtubules to kinetochores during mitosis generates pulling force, or tension, required for the high fidelity of chromosome separation. A lack of tension activates the spindle...
Kang, Dongmin, Chen, James, Wong, Jim, Fang, Guowei
The checkpoint protein Chfr delays entry into mitosis, in the presence of mitotic stress (Scolnick, D.M., and T.D. Halazonetis. 2000. Nature. 406:430–435). We show here that Chfr is a ubiquitin...
Accurate chromosome segregation is controlled by the spindle checkpoint, which senses kinetochore– microtubule attachments and tension across sister kinetochores. An important step in the...
Wong, Jim, Lerrigo, Robert, Jang, Chang-Young, Fang, Guowei
HURP is a spindle-associated protein that mediates Ran-GTP-dependent assembly of the bipolar spindle and promotes chromosome congression and interkinetochore tension during mitosis. We report here a...
Plk1- and β-TrCP–dependent degradation of Bora controls mitotic progression
Seki, Akiko, Coppinger, Judith A., Du, Haining, Jang, Chang-Young, Yates, John R., Fang, Guowei
Through a convergence of functional genomic and proteomic studies, we identify Bora as a previously unknown cell cycle protein that interacts with the Plk1 kinase and the SCF–β-TrCP ubiquitin...
Jang, Chang-Young, Wong, Jim, Coppinger, Judith A., Seki, Akiko, Yates, John R., Fang, Guowei
Dynamic turnover of the spindle is a driving force for chromosome congression and segregation in mitosis. Through a functional genomic analysis, we identify DDA3 as a previously unknown regulator of...
RCS1, a substrate of APC/C, controls the metaphase to anaphase transition
Zhao, Wei-meng, Coppinger, Judith A., Seki, Akiko, Cheng, Xiao-li, Yates, John R., Fang, Guowei
The anaphase-promoting complex/cyclosome (APC/C) controls the onset of anaphase by targeting securin for destruction. We report here the identification and characterization of a substrate of APC/C,...
Zhu, Hui, Coppinger, Judith A., Jang, Chang-Young, Yates, John R., Fang, Guowei
Microtubules (MTs) are nucleated from centrosomes and chromatin. In addition, MTs can be generated from preexiting MTs in a γ-tubulin–dependent manner in yeast, plant, and Drosophila cells,...
Zhu, Hui, Fang, Kayleen, Fang, Guowei
Efficient assembly of a mitotic spindle and stable attachment of microtubules (k-fibers) to kinetochores are essential for the high fidelity of chromosome segregation. Both spindle assembly and...