Ebhardt, H. Alexander, Unrau, Peter J.
Here we present a simple and inexpensive gel-shift assay for the detection and quantification of small RNAs. The assay is at least 5–10 times more sensitive than a conventional Northern, and is...
Ebhardt, H. Alexander, Tsang, Herbert H., Dai, Denny C., Liu, Yifeng, Bostan, Babak, Fahlman, Richard P.
Recent advances in DNA-sequencing technology have made it possible to obtain large datasets of small RNA sequences. Here we demonstrate that not all non-perfectly matched small RNA sequences are...
Comparative analysis of the small RNA transcriptomes of Pinus contorta and Oryza sativa (2008)
Morin, Ryan D., Aksay, Gozde, Dolgosheina, Elena, Ebhardt, H. Alexander, Magrini, Vincent, Mardis, Elaine R., ...
The diversity of microRNAs and small-interfering RNAs has been extensively explored within angiosperms by focusing on a few key organisms such as Oryza sativa and Arabidopsis thaliana. A deeper...
Ebbie: automated analysis and storage of small RNA cloning data using a dynamic web server (2006)
Ebhardt, H Alexander, Wiese, Kay C, Unrau, Peter J
Abstract Background DNA sequencing is used ubiquitously: from deciphering genomes 1 to determining the primary sequence of small RNAs (smRNAs) 2 3 4 5 . The cloning of smRNAs is currently the most...
RNA Secondary Structure Prediction via Energy Density Minimization (2006)
Can Alkan, Emre Karakoc, S. Cenk Sahinalp, Peter Unrau, H. Alexander Ebhardt, H. Alex, ...
There is a resurgence of interest in RNA secondary structure prediction problem (a.k.a. the RNA folding problem) due to the discovery of many new families of non-coding RNAs with a variety of...
Extensive 3′ modification of plant small RNAs is modulated by helper component-proteinase expression
Ebhardt, H. Alexander, Thi, Emily P., Wang, Ming-Bo, Unrau, Peter J.
RNA silencing is an evolutionarily conserved process in eukaryotes that represses gene expression by using 21- to 24-nt guide RNAs to mediate mRNA cleavage or translational inhibition. Plants have...
Extensive 3′ modification of plant small RNAs is modulated by helper component-proteinase expression
Ebhardt, H. Alexander, Thi, Emily P., Wang, Ming-Bo, Unrau, Peter J.
RNA silencing is an evolutionarily conserved process in eukaryotes that represses gene expression by using 21- to 24-nt guide RNAs to mediate mRNA cleavage or translational inhibition. Plants have...
Comparative analysis of the small RNA transcriptomes of Pinus contorta and Oryza sativa
Morin, Ryan D., Aksay, Gozde, Dolgosheina, Elena, Ebhardt, H. Alexander, Magrini, Vincent, Mardis, Elaine R., ...
The diversity of microRNAs and small-interfering RNAs has been extensively explored within angiosperms by focusing on a few key organisms such as Oryza sativa and Arabidopsis thaliana. A deeper...
Ebhardt, H. Alexander, Tsang, Herbert H., Dai, Denny C., Liu, Yifeng, Bostan, Babak, Fahlman, Richard P.
Recent advances in DNA-sequencing technology have made it possible to obtain large datasets of small RNA sequences. Here we demonstrate that not all non-perfectly matched small RNA sequences are...
Ebhardt, H. Alexander, Unrau, Peter J.
Here we present a simple and inexpensive gel-shift assay for the detection and quantification of small RNAs. The assay is at least 5–10 times more sensitive than a conventional Northern, and is...