Mackow, E R, Yamanaka, M Y, Dang, M N, Greenberg, H B
DNA amplification-restricted transcription-translation (DARTT), is based on DNA amplification by the polymerase chain reaction (PCR) and uses PCR to truncate protein-encoding DNA while adding...
Detection and Characterization of Novel Rotavirus Strains in the United States
Ramachandran, M., Gentsch, J. R., Parashar, U. D., Jin, S., Woods, P. A., Holmes, J. L., ...
Detection and Characterization of Novel Rotavirus Strains in the United States
Ramachandran, M., Gentsch, J. R., Parashar, U. D., Jin, S., Woods, P. A., Holmes, J. L., ...
We recently established a rotavirus strain surveillance system in the United States to monitor the prevalent G serotypes before and after the anticipated implementation of a vaccination program...
Heterogeneity of VP4 neutralization epitopes among serotype P1A human rotavirus strains.
Contreras, J F, Menchaca, G E, Padilla-Noriega, L, Tamez, R S, Greenberg, H B, López, S, ...
We have used serotype-specific VP4 and VP7 neutralizing monoclonal antibodies (Nt-MAbs), as well as subgroup (SG)-specific MAbs, to characterize by enzyme immunoassay rotavirus strains isolated from...
Greenberg, H B, Valdesuso, J R, Kalica, A R, Wyatt, R G, McAuliffe, V J, Kapikian, A Z, ...
The proteins of the Norwalk virus were studied by polyacrylamide gel electrophoresis. Highly purified specifically immunoprecipitated virions appeared to contain a single primary structural protein...
Expression of the rotavirus SA11 protein VP7 in the simple eukaryote Dictyostelium discoideum.
Emslie, K R, Miller, J M, Slade, M B, Dormitzer, P R, Greenberg, H B, Williams, K L
The outer capsid protein of rotavirus, VP7, is a major neutralization antigen and is considered a necessary component of any subunit vaccine developed against rotavirus infection. For this reason,...
Rotavirus-induced fusion from without in tissue culture cells.
Falconer, M M, Gilbert, J M, Roper, A M, Greenberg, H B, Gavora, J S
We present the first evidence of fusion from without induced in tissue culture cells by a nonenveloped virus. Electron micrographs of two strains of rotavirus, bovine rotavirus C486 and rhesus...
The immune mechanisms involved in clearance of and immunity to rotavirus infection are poorly understood. Although mice with severe combined immunodeficiency (SCID mice) become chronically infected,...
Genetic mapping indicates that VP4 is the rotavirus cell attachment protein in vitro and in vivo.
Ludert, J E, Feng, N, Yu, J H, Broome, R L, Hoshino, Y, Greenberg, H B
To identify the rotavirus protein which mediates attachment to cells in culture, viral reassortants between the simian rotavirus strain RRV and the murine strains EHP and EW or between the simian...
Franco, M A, Tin, C, Rott, L S, VanCott, J L, McGhee, J R, Greenberg, H B
We recently showed that class I-restricted CD8+ T cells mediate clearance of primary rotavirus infection in mice: JHD knockout (JHD -/-) (B-cell-deficient) mice depleted of CD8+ T cells become...
Franco, M A, Tin, C, Greenberg, H B
We have recently shown that CD8+ T cells mediate clearance of rotavirus infection in mice. B-cell-deficient J(H)D knockout (-/-) mice depleted of CD8+ T cells become chronically infected with murine...
We recently described an assay that measures fusion from without induced in tissue culture cells by rotavirus, a nonenveloped, triple-protein-layered member of the Reoviridae family (M. M. Falconer,...
Ishida, S, Feng, N, Tang, B, Gilbert, J M, Greenberg, H B
The purpose of the present study was to develop a quantitative assay that could be used to measure the local and systemic immune responses to specific rotavirus proteins following rotavirus infection...
Hepatitis C virus detection by single-round PCR specific for the terminal 3' noncoding region.
Umlauft, F, Wong, D T, Oefner, P J, Underhill, P A, Cheung, R C, Wright, T L, ...
A single-round PCR method with primers specific for the 3' noncoding region (NCR) of hepatitis C virus (HCV) has been developed. Using a double RNAzol-B extraction, a high-temperature...
Lewis, T L, Greenberg, H B, Herrmann, J E, Smith, L S, Matsui, S M
We report the results from sequence analysis and expression studies of the gastroenteritis agent astrovirus serotype 1. We have cloned and sequenced 5,944 nucleotides (nt) of the estimated 7.2-kb RNA...
Intracellular manipulation of disulfide bond formation in rotavirus proteins during assembly.
Svensson, L, Dormitzer, P R, Von Bonsdorff, C H, Maunula, L, Greenberg, H B
Rotavirus undergoes a unique mode of assembly in the rough endoplasmic reticulum (RER) of infected cells. Luminal RER proteins undergo significant cotranslational and posttranslational modifications,...
Dubuisson, J, Hsu, H H, Cheung, R C, Greenberg, H B, Russell, D G, Rice, C M
Hepatitis C virus (HCV) encodes two putative virion glycoproteins (E1 and E2) which are released from the polyprotein by signal peptidase cleavage. In this report, we have characterized the complexes...
Feng, N, Burns, J W, Bracy, L, Greenberg, H B
Rotaviruses are the single most important cause of severe diarrhea in young children worldwide, and vaccination is probably the most effective way to control the disease. Most current live virus...
Ward, R L, McNeal, M M, Sander, D S, Greenberg, H B, Bernstein, D I
Natural infection by very similar strains of rotavirus during the 1988-1989 rotavirus season in Cincinnati, Ohio, provided complete protection of young children against subsequent rotavirus illnesses...
Cloning and characterization of human astrovirus immunoreactive epitopes.
Matsui, S M, Kim, J P, Greenberg, H B, Young, L M, Smith, L S, Lewis, T L, ...
We report the cloning of antigenic, protein-coding regions of human astrovirus serotype 1 that appear to be common to most, if not all, serotypes of human astrovirus. Screening of lambda gt11...
Broome, R L, Vo, P T, Ward, R L, Clark, H F, Greenberg, H B
Simian rotavirus (RRV) and murine rotavirus (EDIM-RW) differ dramatically in the oral inoculum required to cause diarrheal disease in neonatal mouse pups and in their ability to spread and cause...
Isolation and characterization of a novel reassortant between avian Ty-1 and simian RRV rotaviruses.
Kool, D A, Matsui, S M, Greenberg, H B, Holmes, I H
A reassortant, TyRh, was isolated after coinfection of MA104 cells with avian Ty-1 and simian RRV rotaviruses. Hybridization and serological studies showed that the reassortant's 4th gene, which...
Two distinct patterns of neutralization were identified by comparing the neutralization curves of monoclonal antibodies (MAbs) directed at the two surface proteins, VP4 and VP7, of rhesus rotavirus....
Dharakul, T, Rott, L, Greenberg, H B
Severe combined immunodeficient (SCID) mice lack both functional T and B cells. These mice develop chronic rotavirus infection following an oral inoculation with the epizootic diarrhea of infant mice...
Mackow, E R, Barnett, J W, Chan, H, Greenberg, H B
Rhesus rotavirus (RRV) gene 4 was cloned into lambda bacteriophage, inserted into a polyhedrin promoter shuttle plasmid, and expressed in Sf9 cells by a recombinant baculovirus. The...
Symmetric infection of rotavirus on polarized human intestinal epithelial (Caco-2) cells.
Svensson, L, Finlay, B B, Bass, D, Von Bonsdorff, C H, Greenberg, H B
When rotavirus infects the mature villus tip cells of the small intestine, it encounters a highly polarized epithelium. In order to understand this virus-cell interaction more completely, we utilized...
Sequence analysis of gene 11 equivalents from "short" and "super short" strains of rotavirus.
Matsui, S M, Mackow, E R, Matsuno, S, Paul, P S, Greenberg, H B
The molecular basis for the aberrant migration pattern of the gene 11 equivalent in rotaviruses with "short" (human DS-1) and "super short" (human 69M and bovine VMRI) electropherotypes was...
NS35 and not vp7 is the soluble rotavirus protein which binds to target cells.
Bass, D M, Mackow, E R, Greenberg, H B
Recent studies using radiolabeled rotavirus lysates have demonstrated a 35-kilodalton viral protein that binds specifically to the surface of MA104 cells (N. Fukuhara, O. Yoshie, S. Kitakoa, and T....
Uhnoo, I, Riepenhoff-Talty, M, Dharakul, T, Chegas, P, Fisher, J E, Greenberg, H B, ...
The pathogenic profiles of two heterologous animal rotaviruses, rhesus rotavirus strain MMU 18006 and bovine rotavirus strain WC3, were evaluated in mice with severe combined immunodeficiency (SCID...
Lack of cosegregation of the subgroup II antigens on genes 2 and 6 in porcine rotaviruses.
Svensson, L, Padilla-Noriega, L, Taniguchi, K, Greenberg, H B
The rotavirus subgroup I and II specificities associated with gene 2 and 6 products (vp2 and vp6, respectively) were shown not to cosegregate in a number of porcine rotavirus strains. The porcine OSU...
Mackow, E R, Vo, P T, Broome, R, Bass, D, Greenberg, H B
A baculovirus-expressed VP4 protein derived from the simian rhesus rotavirus (RRV) was used to parenterally immunize murine dams. VP4-immunized dams developed high levels of neutralizing antibodies...
Ward, R L, Knowlton, D R, Greenberg, H B, Schiff, G M, Bernstein, D I
Serum specimens from infants 2 to 12 months old vaccinated with the WC3 bovine rotavirus were analyzed to determine the relative concentrations of neutralizing antibody to the VP4 and VP7 proteins of...
Dharakul, T, Labbe, M, Cohen, J, Bellamy, A R, Street, J E, Mackow, E R, ...
Clearance of chronic murine rotavirus infection in SCID mice can be demonstrated by adoptive transfer of immune CD8+ T lymphocytes from histocompatible donor mice immunized with a murine homotypic...
Epitope mapping of neutralizing monoclonal antibodies against duck hepatitis B virus.
Cheung, R C, Robinson, W S, Marion, P L, Greenberg, H B
In this article we report the first topological mapping of neutralizing epitopes of a hepadnavirus. Duck hepatitis B virus is the only hepadnavirus that can replicate and spread from cell to cell in...
Offit, P A, Greenberg, H B, Dudzik, K I
We found that rotavirus-specific protein synthesis was not necessary for recognition by virus-specific cytotoxic T lymphocytes (CTLs). In addition, CTLs lysed rotavirus-infected target cells prior to...
Molecular basis of rotavirus virulence: role of gene segment 4.
Offit, P A, Blavat, G, Greenberg, H B, Clark, H F
Bovine rotavirus NCDV and simian rotavirus SA-11 exhibited markedly different patterns of gastrointestinal tract disease when inoculated orally into newborn mice. A genetic approach was used to...
Cultivation and characterization of three strains of murine rotavirus.
Greenberg, H B, Vo, P T, Jones, R
Three distinct strains of murine rotavirus were adapted to growth in cell culture. These strains are genetically related but not identical; they are serotypically heterogeneous. The cultivatable...
Offit, P A, Shaw, R D, Greenberg, H B
Monoclonal antibodies directed against two rotavirus surface proteins (vp3 and vp7) as well as a rotavirus inner capsid protein (vp6) were tested for their ability to protect suckling mice against...
Infectious rotavirus enters cells by direct cell membrane penetration, not by endocytosis.
Kaljot, K T, Shaw, R D, Rubin, D H, Greenberg, H B
Rotaviruses are icosahedral viruses with a segmented, double-stranded RNA genome. They are the major cause of severe infantile infectious diarrhea. Rotavirus growth in tissue culture is markedly...
Ward, R L, Knowlton, D R, Schiff, G M, Hoshino, Y, Greenberg, H B
Two outer capsid rotavirus proteins, VP3 and VP7, have been found to elicit neutralizing-antibody production, but the immunogenicity of these proteins during human rotavirus infection has not been...
Burns, J W, Greenberg, H B, Shaw, R D, Estes, M K
An immunochemical analysis of the hemagglutinin (VP4) of the simian rotavirus SA11 was performed to better understand the structure and function of this molecule. Following immunization of mice with...
Serotypic analysis of VP3 and VP7 neutralization escape mutants of rhesus rotavirus.
Shaw, R D, Mackow, E R, Dyall-Smith, M L, Lazdins, I, Holmes, I H, Greenberg, H B
Neutralization escape mutants of simian rotaviruses (rhesus rotavirus and SA11) were tested in hemagglutination inhibition and neutralization assays against hyperimmune and infection sera to...
Two forms of VP7 are involved in assembly of SA11 rotavirus in endoplasmic reticulum.
Kabcenell, A K, Poruchynsky, M S, Bellamy, A R, Greenberg, H B, Atkinson, P H
Two pools of the glycoprotein VP7 were detected in the endoplasmic reticulum (ER) of SA11 rotavirus-infected cells. One portion of the newly synthesized protein with VP3 composed the virus outer...
Phenotypic mixing during coinfection of cells with two strains of human rotavirus.
Ward, R L, Knowlton, D R, Greenberg, H B
Coinfection of MA-104 cells with serotype 1 (Wa strain) and serotype 3 (P strain) human rotaviruses resulted in progeny viruses that were phenotypically mixed in their major outer-shell...
Reassortant rotaviruses as potential live rotavirus vaccine candidates.
Midthun, K, Greenberg, H B, Hoshino, Y, Kapikian, A Z, Wyatt, R G, Chanock, R M
A series of reassortants was isolated from coinfection of cell cultures with a wild-type animal rotavirus and a "noncultivatable" human rotavirus. Wild-type bovine rotavirus (UK strain) was...
Greenberg, H B, Valdesuso, J, Van Wyke, K, Midthun, K, Walsh, M, McAuliffe, V, ...
A series of monoclonal antibodies was isolated which reacted with one of two major surface proteins of rhesus rotavirus. Thirty-six monoclonal antibodies immunoprecipitated the 82-kilodalton outer...
In vitro transcription of two human rotaviruses.
Flores, J, Myslinski, J, Kalica, A R, Greenberg, H B, Wyatt, R G, Kapikian, A Z, ...
The RNA polymerase activities of a cultivatable (Wa) and a noncultivatable (DS-1) strain of human rotavirus were studied. Under optimal conditions, transcription of all of their RNA segments...
Dunn, S J, Greenberg, H B, Ward, R L, Nakagomi, O, Burns, J W, Vo, P T, ...
Human rotaviruses were isolated from asymptomatic neonates at various hospitals and clinics in the city of Bangalore, India, and were found to be subgroup I specific and possess long RNA patterns (M....
Serologic analysis of human rotavirus serotypes P1A and P2 by using monoclonal antibodies.
Padilla-Noriega, L, Werner-Eckert, R, Mackow, E R, Gorziglia, M, Larralde, G, Taniguchi, K, ...
Three human rotavirus (HRV) VP4 serotypes and one subtype have been described on the basis of a fourfold or an eightfold-or-greater difference in neutralization titer when tested with hyperimmune...
Midthun, K, Greenberg, H B, Kurtz, J B, Gary, G W, Lin, F Y, Kapikian, A Z
The Marin County strain of type 5 astrovirus was associated with two separate outbreaks of nonbacterial gastroenteritis in California in 1978. A safety-tested, bacterium-free filtrate prepared from a...
Rapid and sensitive method for detection of hepatitis C virus RNA by using silica particles.
Cheung, R C, Matsui, S M, Greenberg, H B
We describe a rapid, sensitive, and economic method for detection of hepatitis C virus (HCV) RNA. This method uses silica particles for purification of nucleic acid and then a modified reverse...
Isolation, propagation, and characterization of a second equine rotavirus serotype.
Hoshino, Y, Wyatt, R G, Greenberg, H B, Kalica, A R, Flores, J, Kapikian, A Z
A rotavirus designated strain H-2 was isolated in primary African green monkey kidney cells from a foal with diarrhea. This cell culture-adapted strain was found to be similar, if not identical, to...
Hoshino, Y, Wyatt, R G, Greenberg, H B, Kalica, A R, Flores, J, Kapikian, A Z
By the plaque reduction neutralization test, the CU-1 strain of canine rotavirus was similar, if not identical, to three strains (no. 14, no. 15, and P) of the tentatively designated third human...
Comparisons of rotavirus VP7-typing monoclonal antibodies by competition binding assay.
Raj, P, Matson, D O, Coulson, B S, Bishop, R F, Taniguchi, K, Urasawa, S, ...
Three sets of neutralizing monoclonal antibodies (MAbs) used to type the outer capsid protein VP7 of four group A rotavirus serotypes (1 through 4) were compared in competition immunoassays....
Padilla-Noriega, L, Fiore, L, Rennels, M B, Losonsky, G A, Mackow, E R, Greenberg, H B
The humoral immune response to rhesus rotavirus (RRV) VP4 and its cleavage products VP5* and VP8* was determined in paired serum samples from 44 infants vaccinated with RRV or human rotavirus-RRV...
Svensson, L, Grahnquist, L, Pettersson, C A, Grandien, M, Stintzing, G, Greenberg, H B
Of 126 rotavirus-positive specimens, 7 could not be subgrouped (I or II). These strains showed a distinct reaction with a monoclonal antibody recognizing a common region on VP6, but they did not...
Paul, P S, Lyoo, Y S, Woode, G N, Zheng, S L, Greenberg, H B, Matsui, S, ...
A rotavirus with a "super-short" RNA electropherotype was isolated from a calf with diarrhea and was designated VMRI strain. Segments 10 and 11 of this rotavirus migrated more slowly than did those...
Matsui, S M, Offit, P A, Vo, P T, Mackow, E R, Benfield, D A, Shaw, R D, ...
A murine model was used to determine whether neutralizing monoclonal antibodies (MAbs) with heterotypic specificity directed to VP7 (MAb 57-8) or to the VP8 fragment of VP4 (MAb M14) passively...
Diversity of rotavirus serotypes in Mexican infants with gastroenteritis.
Padilla-Noriega, L, Arias, C F, López, S, Puerto, F, Snodgrass, D R, Taniguchi, K, ...
One hundred thirty-two stool specimens from infants with rotavirus gastroenteritis hospitalized in two Mexican cities (Mexico City and Mérida) were examined by serotype- and subgroup-specific enzyme...
Specific enzyme-linked immunoassay for rotavirus serotypes 1 and 3.
Shaw, R D, Stoner-Ma, D L, Estes, M K, Greenberg, H B
We prepared monoclonal antibodies against two serotypically distinct rotavirus strains: Wa, a serotype 1 virus of human origin, and rhesus rotavirus, a simian serotype 3 virus. Monoclonal antibodies...
Rotavirus serotypes 6 and 10 predominate in cattle.
Snodgrass, D R, Fitzgerald, T, Campbell, I, Scott, F M, Browning, G F, Miller, D L, ...
Calf fecal rotavirus strains were serotyped in enzyme-linked immunosorbent assays, using monoclonal antibodies to the VP7s of serotypes 1, 2, 3, 5, and 6 and to the VP4 of B223 (designated serotype...
De Leon, R, Matsui, S M, Baric, R S, Herrmann, J E, Blacklow, N R, Greenberg, H B, ...
A reverse transcriptase (RT)-polymerase chain reaction (PCR)-oligoprobe (OP), or RT-PCR-OP, method was developed for the detection of the Norwalk virus, which causes acute, epidemic gastroenteritis,...
Wyatt, R G, James, H D, Pittman, A L, Hoshino, Y, Greenberg, H B, Kalica, A R, ...
Of 73 rotavirus-positive fecal specimens tested, 39 yielded a human rotavirus that could be cultivated serially in MA104 or primary African green monkey kidney cells or both; 18 were serotyped. Four...
Isolation and characterization of an equine rotavirus.
Hoshino, Y, Wyatt, R G, Greenberg, H B, Kalica, A R, Flores, J, Kapikian, A Z
A rotavirus, designated as the H-1 strain, was isolated from a diarrheic foal in primary African green monkey kidney cells and MA104 cells. This cell culture-adapted strain hemagglutinated...
Enhancement of antigen incorporation and infectivity of cell cultures by human rotavirus.
Schoub, B D, Kalica, A R, Greenberg, H B, Bertran, D M, Sereno, M M, Wyatt, R G, ...
Infection of cell cultures with human rotavirus preparations was attempted and the effects of trypsin and low-speed centrifugation on antigen incorporation, as demonstrated by immunofluorescence and...
Blacklow, N R, Cukor, G, Bedigian, M K, Echeverria, P, Greenberg, H B, Schreiber, D S, ...
A solid-phase microtiter radioimmunoassay was established for the detection of Norwalk virus and its antibody, with clinical materials from human volunteers previously studied in Massachusetts as...
Enzyme-linked immunosorbent assay for detection of Escherichia coli heat-labile enterotoxin.
Yolken, R H, Greenberg, H B, Merson, M H, Sack, R B, Kapikian, A Z
The development of an enzyme-linked immunosorbent assay (ELISA) for the detection of heat-labile Escherichia coli enterotoxin is described. The assay, which is based on the immunological similarity...
Mackow, E R, Shaw, R D, Matsui, S M, Vo, P T, Dang, M N, Greenberg, H B
The complete gene 4 nucleotide sequence was determined for rhesus rotavirus and each of 11 viral variants selected by neutralizing monoclonal antibodies. Gene 4 is 2362 bases in length and encodes a...
Offit, P A, Clark, H F, Blavat, G, Greenberg, H B
Genetic studies of reassortant rotaviruses have demonstrated that gene segments 4 and 9 each segregate with the serotype-specific neutralization phenotype in vitro. Reassortant rotaviruses derived by...
The isolation and characterization of a Norwalk virus-specific cDNA.
Matsui, S M, Kim, J P, Greenberg, H B, Su, W, Sun, Q, Johnson, P C, ...
Norwalk virus, an important cause of epidemic, acute, nonbacterial gastroenteritis in adults and children, has eluded adaptation to tissue culture, the development of an animal model, and molecular...
Molecular basis of age-dependent gastric inactivation of rhesus rotavirus in the mouse.
Bass, D M, Baylor, M, Broome, R, Greenberg, H B
Rotavirus requires specific proteolytic activation by trypsin for efficient replication in tissue culture. To observe the nature of intestinal proteolytic activation of rotavirus in vivo,...
Greenberg, H B, Kalica, A R, Wyatt, R G, Jones, R W, Kapikian, A Z, Chanock, R M
Fastidious human rotaviruses that did not undergo productive infection in tissue culture were rescued by genetic reassortment during mixed infection with a temperature-sensitive (ts) mutant of a...
Rescue and serotypic characterization of noncultivable human rotavirus by gene reassortment.
Greenberg, H B, Wyatt, R G, Kapikian, A Z, Kalica, A R, Flores, J, Jones, R
Thirty-three of 50 noncultivable human rotavirus strains from a variety of locations were successfully rescued by gene reassortment. The serotype of each of the 33 strains was investigated by a...
Definition of human rotavirus serotypes by plaque reduction assay.
Wyatt, R G, Greenberg, H B, James, W D, Pittman, A L, Kalica, A R, Flores, J, ...
Twenty different human rotavirus reassortants were characterized serologically by a plaque reduction assay as belonging to one of three distinct serotypes. Fourteen were similar if not identical to...
Kapikian, A Z, Cline, W L, Greenberg, H B, Wyatt, R G, Kalica, A R, Banks, C E, ...
An immune adherence hemagglutination assay (IAHA) and a modified enzyme-linked immunosorbent assay for antigenic characterization of human rotaviruses were developed. The designations of type 1 and...
Distinctive ribonucleic acid patterns of human rotavirus subgroups 1 and 2.
Kalica, A R, Greenberg, H B, Espejo, R T, Flores, J, Wyatt, R G, Kapikian, A Z, ...
The ribonucleic acid migration patterns of 7 subgroup 1 and 16 subgroup 2 human rotaviruses recovered from four geographic areas were compared. The subgroup 1 ribonucleic acid patterns had strikingly...
Prevalence of antibody to the Norwalk virus in various countries.
Greenberg, H B, Valdesuso, J, Kapikian, A Z, Chanock, R M, Wyatt, R G, Szmuness, W, ...
Serum samples from children and adults from several countries were tested by radioimmunoassay for antibody to the Norwalk virus. Antibody was commonly found in adults from all the countries tested....
Microtiter solid-phase radioimmunoassay for detection of Escherichia coli heat-labile enterotoxin.
Greenberg, H B, Sack, D A, Rodriguez, W, Sack, R B, Wyatt, R G, Kalica, A R, ...
The development of a microtiter solid-phase radioimmunoassay for the detection of Vibrio cholerae enterotoxin and heat-labile Escherichia coli enterotoxin is described. The test is based on the...
Bass, D M, Baylor, M R, Chen, C, Mackow, E M, Bremont, M, Greenberg, H B
Rotaviruses are an important cause of gastroenteritis in human infants. In vivo, rotavirus displays striking cell tropism with viral replication generally restricted to the villus tip enterocytes of...
Rott, L S, Rosé, J R, Bass, D, Williams, M B, Greenberg, H B, Butcher, E C
The integrin alpha4beta7 mediates lymphocyte binding to mucosal addressin cell adhesion molecule-1, and its expression defines lymphocytes capable of trafficking through the intestines and the...
Detection of Escherichia coli enterotoxins in stools.
Merson, M H, Yolken, R H, Sack, R B, Froehlich, J L, Greenberg, H B, Huq, I, ...
We determined whether enterotoxigenic Escherichia coli diarrhea could be diagnosed by direct examination of stools for heat-labile (LT) and heat-stable (ST) enterotoxins. The Y-1 adrenal cell and an...
Hepatitis G virus: is it a hepatitis virus?
Cheung, R C, Keeffe, E B, Greenberg, H B
Hepatitis G virus (HGV) and GB virus C (GBV-C) are two newly discovered viral agents, different isolates of a positive-sense RNA virus that represents a new genus of Flaviviridae. The purpose of this...
Fenaux, M., Cuadras, M. A., Feng, N., Jaimes, M., Greenberg, H. B.
Although rotavirus infection has generally been felt to be restricted to the gastrointestinal tract, over the last two decades there have been sporadic reports of children with acute or fatal cases...
Use of the brief MAST interview to detect alcoholics in a tuberculous population.
Bailey, W C, Horwitz, J, Brown, M, Thompson, D H, Ziskind, M M, Greenberg, H B
Mackow, E R, Yamanaka, M Y, Dang, M N, Greenberg, H B
DNA amplification-restricted transcription-translation (DARTT), is based on DNA amplification by the polymerase chain reaction (PCR) and uses PCR to truncate protein-encoding DNA while adding...
Detection and Characterization of Novel Rotavirus Strains in the United States
Ramachandran, M., Gentsch, J. R., Parashar, U. D., Jin, S., Woods, P. A., Holmes, J. L., ...
Detection and Characterization of Novel Rotavirus Strains in the United States
Ramachandran, M., Gentsch, J. R., Parashar, U. D., Jin, S., Woods, P. A., Holmes, J. L., ...
We recently established a rotavirus strain surveillance system in the United States to monitor the prevalent G serotypes before and after the anticipated implementation of a vaccination program...
Heterogeneity of VP4 neutralization epitopes among serotype P1A human rotavirus strains.
Contreras, J F, Menchaca, G E, Padilla-Noriega, L, Tamez, R S, Greenberg, H B, López, S, ...
We have used serotype-specific VP4 and VP7 neutralizing monoclonal antibodies (Nt-MAbs), as well as subgroup (SG)-specific MAbs, to characterize by enzyme immunoassay rotavirus strains isolated from...
Greenberg, H B, Valdesuso, J R, Kalica, A R, Wyatt, R G, McAuliffe, V J, Kapikian, A Z, ...
The proteins of the Norwalk virus were studied by polyacrylamide gel electrophoresis. Highly purified specifically immunoprecipitated virions appeared to contain a single primary structural protein...
Expression of the rotavirus SA11 protein VP7 in the simple eukaryote Dictyostelium discoideum.
Emslie, K R, Miller, J M, Slade, M B, Dormitzer, P R, Greenberg, H B, Williams, K L
The outer capsid protein of rotavirus, VP7, is a major neutralization antigen and is considered a necessary component of any subunit vaccine developed against rotavirus infection. For this reason,...
Rotavirus-induced fusion from without in tissue culture cells.
Falconer, M M, Gilbert, J M, Roper, A M, Greenberg, H B, Gavora, J S
We present the first evidence of fusion from without induced in tissue culture cells by a nonenveloped virus. Electron micrographs of two strains of rotavirus, bovine rotavirus C486 and rhesus...
The immune mechanisms involved in clearance of and immunity to rotavirus infection are poorly understood. Although mice with severe combined immunodeficiency (SCID mice) become chronically infected,...
Genetic mapping indicates that VP4 is the rotavirus cell attachment protein in vitro and in vivo.
Ludert, J E, Feng, N, Yu, J H, Broome, R L, Hoshino, Y, Greenberg, H B
To identify the rotavirus protein which mediates attachment to cells in culture, viral reassortants between the simian rotavirus strain RRV and the murine strains EHP and EW or between the simian...
Franco, M A, Tin, C, Rott, L S, VanCott, J L, McGhee, J R, Greenberg, H B
We recently showed that class I-restricted CD8+ T cells mediate clearance of primary rotavirus infection in mice: JHD knockout (JHD -/-) (B-cell-deficient) mice depleted of CD8+ T cells become...
Franco, M A, Tin, C, Greenberg, H B
We have recently shown that CD8+ T cells mediate clearance of rotavirus infection in mice. B-cell-deficient J(H)D knockout (-/-) mice depleted of CD8+ T cells become chronically infected with murine...
We recently described an assay that measures fusion from without induced in tissue culture cells by rotavirus, a nonenveloped, triple-protein-layered member of the Reoviridae family (M. M. Falconer,...
Ishida, S, Feng, N, Tang, B, Gilbert, J M, Greenberg, H B
The purpose of the present study was to develop a quantitative assay that could be used to measure the local and systemic immune responses to specific rotavirus proteins following rotavirus infection...
Hepatitis C virus detection by single-round PCR specific for the terminal 3' noncoding region.
Umlauft, F, Wong, D T, Oefner, P J, Underhill, P A, Cheung, R C, Wright, T L, ...
A single-round PCR method with primers specific for the 3' noncoding region (NCR) of hepatitis C virus (HCV) has been developed. Using a double RNAzol-B extraction, a high-temperature...
Lewis, T L, Greenberg, H B, Herrmann, J E, Smith, L S, Matsui, S M
We report the results from sequence analysis and expression studies of the gastroenteritis agent astrovirus serotype 1. We have cloned and sequenced 5,944 nucleotides (nt) of the estimated 7.2-kb RNA...
Intracellular manipulation of disulfide bond formation in rotavirus proteins during assembly.
Svensson, L, Dormitzer, P R, Von Bonsdorff, C H, Maunula, L, Greenberg, H B
Rotavirus undergoes a unique mode of assembly in the rough endoplasmic reticulum (RER) of infected cells. Luminal RER proteins undergo significant cotranslational and posttranslational modifications,...
Dubuisson, J, Hsu, H H, Cheung, R C, Greenberg, H B, Russell, D G, Rice, C M
Hepatitis C virus (HCV) encodes two putative virion glycoproteins (E1 and E2) which are released from the polyprotein by signal peptidase cleavage. In this report, we have characterized the complexes...
Feng, N, Burns, J W, Bracy, L, Greenberg, H B
Rotaviruses are the single most important cause of severe diarrhea in young children worldwide, and vaccination is probably the most effective way to control the disease. Most current live virus...
Ward, R L, McNeal, M M, Sander, D S, Greenberg, H B, Bernstein, D I
Natural infection by very similar strains of rotavirus during the 1988-1989 rotavirus season in Cincinnati, Ohio, provided complete protection of young children against subsequent rotavirus illnesses...
Cloning and characterization of human astrovirus immunoreactive epitopes.
Matsui, S M, Kim, J P, Greenberg, H B, Young, L M, Smith, L S, Lewis, T L, ...
We report the cloning of antigenic, protein-coding regions of human astrovirus serotype 1 that appear to be common to most, if not all, serotypes of human astrovirus. Screening of lambda gt11...
Broome, R L, Vo, P T, Ward, R L, Clark, H F, Greenberg, H B
Simian rotavirus (RRV) and murine rotavirus (EDIM-RW) differ dramatically in the oral inoculum required to cause diarrheal disease in neonatal mouse pups and in their ability to spread and cause...
Isolation and characterization of a novel reassortant between avian Ty-1 and simian RRV rotaviruses.
Kool, D A, Matsui, S M, Greenberg, H B, Holmes, I H
A reassortant, TyRh, was isolated after coinfection of MA104 cells with avian Ty-1 and simian RRV rotaviruses. Hybridization and serological studies showed that the reassortant's 4th gene, which...
Two distinct patterns of neutralization were identified by comparing the neutralization curves of monoclonal antibodies (MAbs) directed at the two surface proteins, VP4 and VP7, of rhesus rotavirus....
Dharakul, T, Rott, L, Greenberg, H B
Severe combined immunodeficient (SCID) mice lack both functional T and B cells. These mice develop chronic rotavirus infection following an oral inoculation with the epizootic diarrhea of infant mice...
Mackow, E R, Barnett, J W, Chan, H, Greenberg, H B
Rhesus rotavirus (RRV) gene 4 was cloned into lambda bacteriophage, inserted into a polyhedrin promoter shuttle plasmid, and expressed in Sf9 cells by a recombinant baculovirus. The...
Symmetric infection of rotavirus on polarized human intestinal epithelial (Caco-2) cells.
Svensson, L, Finlay, B B, Bass, D, Von Bonsdorff, C H, Greenberg, H B
When rotavirus infects the mature villus tip cells of the small intestine, it encounters a highly polarized epithelium. In order to understand this virus-cell interaction more completely, we utilized...
Sequence analysis of gene 11 equivalents from "short" and "super short" strains of rotavirus.
Matsui, S M, Mackow, E R, Matsuno, S, Paul, P S, Greenberg, H B
The molecular basis for the aberrant migration pattern of the gene 11 equivalent in rotaviruses with "short" (human DS-1) and "super short" (human 69M and bovine VMRI) electropherotypes was...
NS35 and not vp7 is the soluble rotavirus protein which binds to target cells.
Bass, D M, Mackow, E R, Greenberg, H B
Recent studies using radiolabeled rotavirus lysates have demonstrated a 35-kilodalton viral protein that binds specifically to the surface of MA104 cells (N. Fukuhara, O. Yoshie, S. Kitakoa, and T....
Uhnoo, I, Riepenhoff-Talty, M, Dharakul, T, Chegas, P, Fisher, J E, Greenberg, H B, ...
The pathogenic profiles of two heterologous animal rotaviruses, rhesus rotavirus strain MMU 18006 and bovine rotavirus strain WC3, were evaluated in mice with severe combined immunodeficiency (SCID...
Lack of cosegregation of the subgroup II antigens on genes 2 and 6 in porcine rotaviruses.
Svensson, L, Padilla-Noriega, L, Taniguchi, K, Greenberg, H B
The rotavirus subgroup I and II specificities associated with gene 2 and 6 products (vp2 and vp6, respectively) were shown not to cosegregate in a number of porcine rotavirus strains. The porcine OSU...
Mackow, E R, Vo, P T, Broome, R, Bass, D, Greenberg, H B
A baculovirus-expressed VP4 protein derived from the simian rhesus rotavirus (RRV) was used to parenterally immunize murine dams. VP4-immunized dams developed high levels of neutralizing antibodies...
Ward, R L, Knowlton, D R, Greenberg, H B, Schiff, G M, Bernstein, D I
Serum specimens from infants 2 to 12 months old vaccinated with the WC3 bovine rotavirus were analyzed to determine the relative concentrations of neutralizing antibody to the VP4 and VP7 proteins of...
Dharakul, T, Labbe, M, Cohen, J, Bellamy, A R, Street, J E, Mackow, E R, ...
Clearance of chronic murine rotavirus infection in SCID mice can be demonstrated by adoptive transfer of immune CD8+ T lymphocytes from histocompatible donor mice immunized with a murine homotypic...
Epitope mapping of neutralizing monoclonal antibodies against duck hepatitis B virus.
Cheung, R C, Robinson, W S, Marion, P L, Greenberg, H B
In this article we report the first topological mapping of neutralizing epitopes of a hepadnavirus. Duck hepatitis B virus is the only hepadnavirus that can replicate and spread from cell to cell in...
Offit, P A, Greenberg, H B, Dudzik, K I
We found that rotavirus-specific protein synthesis was not necessary for recognition by virus-specific cytotoxic T lymphocytes (CTLs). In addition, CTLs lysed rotavirus-infected target cells prior to...
Molecular basis of rotavirus virulence: role of gene segment 4.
Offit, P A, Blavat, G, Greenberg, H B, Clark, H F
Bovine rotavirus NCDV and simian rotavirus SA-11 exhibited markedly different patterns of gastrointestinal tract disease when inoculated orally into newborn mice. A genetic approach was used to...
Cultivation and characterization of three strains of murine rotavirus.
Greenberg, H B, Vo, P T, Jones, R
Three distinct strains of murine rotavirus were adapted to growth in cell culture. These strains are genetically related but not identical; they are serotypically heterogeneous. The cultivatable...
Offit, P A, Shaw, R D, Greenberg, H B
Monoclonal antibodies directed against two rotavirus surface proteins (vp3 and vp7) as well as a rotavirus inner capsid protein (vp6) were tested for their ability to protect suckling mice against...
Infectious rotavirus enters cells by direct cell membrane penetration, not by endocytosis.
Kaljot, K T, Shaw, R D, Rubin, D H, Greenberg, H B
Rotaviruses are icosahedral viruses with a segmented, double-stranded RNA genome. They are the major cause of severe infantile infectious diarrhea. Rotavirus growth in tissue culture is markedly...
Ward, R L, Knowlton, D R, Schiff, G M, Hoshino, Y, Greenberg, H B
Two outer capsid rotavirus proteins, VP3 and VP7, have been found to elicit neutralizing-antibody production, but the immunogenicity of these proteins during human rotavirus infection has not been...
Burns, J W, Greenberg, H B, Shaw, R D, Estes, M K
An immunochemical analysis of the hemagglutinin (VP4) of the simian rotavirus SA11 was performed to better understand the structure and function of this molecule. Following immunization of mice with...
Serotypic analysis of VP3 and VP7 neutralization escape mutants of rhesus rotavirus.
Shaw, R D, Mackow, E R, Dyall-Smith, M L, Lazdins, I, Holmes, I H, Greenberg, H B
Neutralization escape mutants of simian rotaviruses (rhesus rotavirus and SA11) were tested in hemagglutination inhibition and neutralization assays against hyperimmune and infection sera to...
Two forms of VP7 are involved in assembly of SA11 rotavirus in endoplasmic reticulum.
Kabcenell, A K, Poruchynsky, M S, Bellamy, A R, Greenberg, H B, Atkinson, P H
Two pools of the glycoprotein VP7 were detected in the endoplasmic reticulum (ER) of SA11 rotavirus-infected cells. One portion of the newly synthesized protein with VP3 composed the virus outer...
Phenotypic mixing during coinfection of cells with two strains of human rotavirus.
Ward, R L, Knowlton, D R, Greenberg, H B
Coinfection of MA-104 cells with serotype 1 (Wa strain) and serotype 3 (P strain) human rotaviruses resulted in progeny viruses that were phenotypically mixed in their major outer-shell...
Reassortant rotaviruses as potential live rotavirus vaccine candidates.
Midthun, K, Greenberg, H B, Hoshino, Y, Kapikian, A Z, Wyatt, R G, Chanock, R M
A series of reassortants was isolated from coinfection of cell cultures with a wild-type animal rotavirus and a "noncultivatable" human rotavirus. Wild-type bovine rotavirus (UK strain) was...
Greenberg, H B, Valdesuso, J, Van Wyke, K, Midthun, K, Walsh, M, McAuliffe, V, ...
A series of monoclonal antibodies was isolated which reacted with one of two major surface proteins of rhesus rotavirus. Thirty-six monoclonal antibodies immunoprecipitated the 82-kilodalton outer...
In vitro transcription of two human rotaviruses.
Flores, J, Myslinski, J, Kalica, A R, Greenberg, H B, Wyatt, R G, Kapikian, A Z, ...
The RNA polymerase activities of a cultivatable (Wa) and a noncultivatable (DS-1) strain of human rotavirus were studied. Under optimal conditions, transcription of all of their RNA segments...
Dunn, S J, Greenberg, H B, Ward, R L, Nakagomi, O, Burns, J W, Vo, P T, ...
Human rotaviruses were isolated from asymptomatic neonates at various hospitals and clinics in the city of Bangalore, India, and were found to be subgroup I specific and possess long RNA patterns (M....
Serologic analysis of human rotavirus serotypes P1A and P2 by using monoclonal antibodies.
Padilla-Noriega, L, Werner-Eckert, R, Mackow, E R, Gorziglia, M, Larralde, G, Taniguchi, K, ...
Three human rotavirus (HRV) VP4 serotypes and one subtype have been described on the basis of a fourfold or an eightfold-or-greater difference in neutralization titer when tested with hyperimmune...
Midthun, K, Greenberg, H B, Kurtz, J B, Gary, G W, Lin, F Y, Kapikian, A Z
The Marin County strain of type 5 astrovirus was associated with two separate outbreaks of nonbacterial gastroenteritis in California in 1978. A safety-tested, bacterium-free filtrate prepared from a...
Rapid and sensitive method for detection of hepatitis C virus RNA by using silica particles.
Cheung, R C, Matsui, S M, Greenberg, H B
We describe a rapid, sensitive, and economic method for detection of hepatitis C virus (HCV) RNA. This method uses silica particles for purification of nucleic acid and then a modified reverse...
Isolation, propagation, and characterization of a second equine rotavirus serotype.
Hoshino, Y, Wyatt, R G, Greenberg, H B, Kalica, A R, Flores, J, Kapikian, A Z
A rotavirus designated strain H-2 was isolated in primary African green monkey kidney cells from a foal with diarrhea. This cell culture-adapted strain was found to be similar, if not identical, to...
Hoshino, Y, Wyatt, R G, Greenberg, H B, Kalica, A R, Flores, J, Kapikian, A Z
By the plaque reduction neutralization test, the CU-1 strain of canine rotavirus was similar, if not identical, to three strains (no. 14, no. 15, and P) of the tentatively designated third human...
Comparisons of rotavirus VP7-typing monoclonal antibodies by competition binding assay.
Raj, P, Matson, D O, Coulson, B S, Bishop, R F, Taniguchi, K, Urasawa, S, ...
Three sets of neutralizing monoclonal antibodies (MAbs) used to type the outer capsid protein VP7 of four group A rotavirus serotypes (1 through 4) were compared in competition immunoassays....
Padilla-Noriega, L, Fiore, L, Rennels, M B, Losonsky, G A, Mackow, E R, Greenberg, H B
The humoral immune response to rhesus rotavirus (RRV) VP4 and its cleavage products VP5* and VP8* was determined in paired serum samples from 44 infants vaccinated with RRV or human rotavirus-RRV...
Svensson, L, Grahnquist, L, Pettersson, C A, Grandien, M, Stintzing, G, Greenberg, H B
Of 126 rotavirus-positive specimens, 7 could not be subgrouped (I or II). These strains showed a distinct reaction with a monoclonal antibody recognizing a common region on VP6, but they did not...
Paul, P S, Lyoo, Y S, Woode, G N, Zheng, S L, Greenberg, H B, Matsui, S, ...
A rotavirus with a "super-short" RNA electropherotype was isolated from a calf with diarrhea and was designated VMRI strain. Segments 10 and 11 of this rotavirus migrated more slowly than did those...
Matsui, S M, Offit, P A, Vo, P T, Mackow, E R, Benfield, D A, Shaw, R D, ...
A murine model was used to determine whether neutralizing monoclonal antibodies (MAbs) with heterotypic specificity directed to VP7 (MAb 57-8) or to the VP8 fragment of VP4 (MAb M14) passively...
Diversity of rotavirus serotypes in Mexican infants with gastroenteritis.
Padilla-Noriega, L, Arias, C F, López, S, Puerto, F, Snodgrass, D R, Taniguchi, K, ...
One hundred thirty-two stool specimens from infants with rotavirus gastroenteritis hospitalized in two Mexican cities (Mexico City and Mérida) were examined by serotype- and subgroup-specific enzyme...
Specific enzyme-linked immunoassay for rotavirus serotypes 1 and 3.
Shaw, R D, Stoner-Ma, D L, Estes, M K, Greenberg, H B
We prepared monoclonal antibodies against two serotypically distinct rotavirus strains: Wa, a serotype 1 virus of human origin, and rhesus rotavirus, a simian serotype 3 virus. Monoclonal antibodies...
Rotavirus serotypes 6 and 10 predominate in cattle.
Snodgrass, D R, Fitzgerald, T, Campbell, I, Scott, F M, Browning, G F, Miller, D L, ...
Calf fecal rotavirus strains were serotyped in enzyme-linked immunosorbent assays, using monoclonal antibodies to the VP7s of serotypes 1, 2, 3, 5, and 6 and to the VP4 of B223 (designated serotype...
De Leon, R, Matsui, S M, Baric, R S, Herrmann, J E, Blacklow, N R, Greenberg, H B, ...
A reverse transcriptase (RT)-polymerase chain reaction (PCR)-oligoprobe (OP), or RT-PCR-OP, method was developed for the detection of the Norwalk virus, which causes acute, epidemic gastroenteritis,...
Wyatt, R G, James, H D, Pittman, A L, Hoshino, Y, Greenberg, H B, Kalica, A R, ...
Of 73 rotavirus-positive fecal specimens tested, 39 yielded a human rotavirus that could be cultivated serially in MA104 or primary African green monkey kidney cells or both; 18 were serotyped. Four...
Isolation and characterization of an equine rotavirus.
Hoshino, Y, Wyatt, R G, Greenberg, H B, Kalica, A R, Flores, J, Kapikian, A Z
A rotavirus, designated as the H-1 strain, was isolated from a diarrheic foal in primary African green monkey kidney cells and MA104 cells. This cell culture-adapted strain hemagglutinated...
Enhancement of antigen incorporation and infectivity of cell cultures by human rotavirus.
Schoub, B D, Kalica, A R, Greenberg, H B, Bertran, D M, Sereno, M M, Wyatt, R G, ...
Infection of cell cultures with human rotavirus preparations was attempted and the effects of trypsin and low-speed centrifugation on antigen incorporation, as demonstrated by immunofluorescence and...
Blacklow, N R, Cukor, G, Bedigian, M K, Echeverria, P, Greenberg, H B, Schreiber, D S, ...
A solid-phase microtiter radioimmunoassay was established for the detection of Norwalk virus and its antibody, with clinical materials from human volunteers previously studied in Massachusetts as...
Enzyme-linked immunosorbent assay for detection of Escherichia coli heat-labile enterotoxin.
Yolken, R H, Greenberg, H B, Merson, M H, Sack, R B, Kapikian, A Z
The development of an enzyme-linked immunosorbent assay (ELISA) for the detection of heat-labile Escherichia coli enterotoxin is described. The assay, which is based on the immunological similarity...
Mackow, E R, Shaw, R D, Matsui, S M, Vo, P T, Dang, M N, Greenberg, H B
The complete gene 4 nucleotide sequence was determined for rhesus rotavirus and each of 11 viral variants selected by neutralizing monoclonal antibodies. Gene 4 is 2362 bases in length and encodes a...
Offit, P A, Clark, H F, Blavat, G, Greenberg, H B
Genetic studies of reassortant rotaviruses have demonstrated that gene segments 4 and 9 each segregate with the serotype-specific neutralization phenotype in vitro. Reassortant rotaviruses derived by...
The isolation and characterization of a Norwalk virus-specific cDNA.
Matsui, S M, Kim, J P, Greenberg, H B, Su, W, Sun, Q, Johnson, P C, ...
Norwalk virus, an important cause of epidemic, acute, nonbacterial gastroenteritis in adults and children, has eluded adaptation to tissue culture, the development of an animal model, and molecular...
Molecular basis of age-dependent gastric inactivation of rhesus rotavirus in the mouse.
Bass, D M, Baylor, M, Broome, R, Greenberg, H B
Rotavirus requires specific proteolytic activation by trypsin for efficient replication in tissue culture. To observe the nature of intestinal proteolytic activation of rotavirus in vivo,...
Greenberg, H B, Kalica, A R, Wyatt, R G, Jones, R W, Kapikian, A Z, Chanock, R M
Fastidious human rotaviruses that did not undergo productive infection in tissue culture were rescued by genetic reassortment during mixed infection with a temperature-sensitive (ts) mutant of a...
Rescue and serotypic characterization of noncultivable human rotavirus by gene reassortment.
Greenberg, H B, Wyatt, R G, Kapikian, A Z, Kalica, A R, Flores, J, Jones, R
Thirty-three of 50 noncultivable human rotavirus strains from a variety of locations were successfully rescued by gene reassortment. The serotype of each of the 33 strains was investigated by a...
Definition of human rotavirus serotypes by plaque reduction assay.
Wyatt, R G, Greenberg, H B, James, W D, Pittman, A L, Kalica, A R, Flores, J, ...
Twenty different human rotavirus reassortants were characterized serologically by a plaque reduction assay as belonging to one of three distinct serotypes. Fourteen were similar if not identical to...
Kapikian, A Z, Cline, W L, Greenberg, H B, Wyatt, R G, Kalica, A R, Banks, C E, ...
An immune adherence hemagglutination assay (IAHA) and a modified enzyme-linked immunosorbent assay for antigenic characterization of human rotaviruses were developed. The designations of type 1 and...
Distinctive ribonucleic acid patterns of human rotavirus subgroups 1 and 2.
Kalica, A R, Greenberg, H B, Espejo, R T, Flores, J, Wyatt, R G, Kapikian, A Z, ...
The ribonucleic acid migration patterns of 7 subgroup 1 and 16 subgroup 2 human rotaviruses recovered from four geographic areas were compared. The subgroup 1 ribonucleic acid patterns had strikingly...
Prevalence of antibody to the Norwalk virus in various countries.
Greenberg, H B, Valdesuso, J, Kapikian, A Z, Chanock, R M, Wyatt, R G, Szmuness, W, ...
Serum samples from children and adults from several countries were tested by radioimmunoassay for antibody to the Norwalk virus. Antibody was commonly found in adults from all the countries tested....
Microtiter solid-phase radioimmunoassay for detection of Escherichia coli heat-labile enterotoxin.
Greenberg, H B, Sack, D A, Rodriguez, W, Sack, R B, Wyatt, R G, Kalica, A R, ...
The development of a microtiter solid-phase radioimmunoassay for the detection of Vibrio cholerae enterotoxin and heat-labile Escherichia coli enterotoxin is described. The test is based on the...
Bass, D M, Baylor, M R, Chen, C, Mackow, E M, Bremont, M, Greenberg, H B
Rotaviruses are an important cause of gastroenteritis in human infants. In vivo, rotavirus displays striking cell tropism with viral replication generally restricted to the villus tip enterocytes of...
Rott, L S, Rosé, J R, Bass, D, Williams, M B, Greenberg, H B, Butcher, E C
The integrin alpha4beta7 mediates lymphocyte binding to mucosal addressin cell adhesion molecule-1, and its expression defines lymphocytes capable of trafficking through the intestines and the...
Detection of Escherichia coli enterotoxins in stools.
Merson, M H, Yolken, R H, Sack, R B, Froehlich, J L, Greenberg, H B, Huq, I, ...
We determined whether enterotoxigenic Escherichia coli diarrhea could be diagnosed by direct examination of stools for heat-labile (LT) and heat-stable (ST) enterotoxins. The Y-1 adrenal cell and an...
Hepatitis G virus: is it a hepatitis virus?
Cheung, R C, Keeffe, E B, Greenberg, H B
Hepatitis G virus (HGV) and GB virus C (GBV-C) are two newly discovered viral agents, different isolates of a positive-sense RNA virus that represents a new genus of Flaviviridae. The purpose of this...
Fenaux, M., Cuadras, M. A., Feng, N., Jaimes, M., Greenberg, H. B.
Although rotavirus infection has generally been felt to be restricted to the gastrointestinal tract, over the last two decades there have been sporadic reports of children with acute or fatal cases...
Use of the brief MAST interview to detect alcoholics in a tuberculous population.
Bailey, W C, Horwitz, J, Brown, M, Thompson, D H, Ziskind, M M, Greenberg, H B
Feng, N., Kim, B., Fenaux, M., Nguyen, H., Vo, P., Omary, M. B., ...
Recent studies demonstrated that viremia and extraintestinal rotavirus infection are common in acutely infected humans and animals, while systemic diseases appear to be rare. Intraperitoneal...