J. Howe, W. Richter, L. Hawkins, M. Roessle, C. Alexander, ...
Journal article
Staubach, K-H, Nolde, J, Block, K, Woltmann, A, Brade, H
No abstract available.
Staubach, K-H, Nolde, J, Song, L, Brade, H, Bruch, H-P
No abstract available.
Persson, K., Osser, S., Birkelund, S., Christiansen, G., Brade, H.
The antibody response to heat shock proteins 60 and 10 were studied in 163 patients with tubal factor infertility and in 163 age-matched pregnant women. The associations of these antibodies with...
Staubach, K, Weber, H, Song, L, Sabranski, M, Brade, H, Bruch, HP
No abstract available.
Schletter, J, Brade, H, Brade, L, Krüger, C, Loppnow, H, Kusumoto, S, ...
Activation of cells by bacterial lipopolysaccharide (LPS) plays a key role in the pathogenesis of gram-negative septic shock. The 55-kDa glycoprotein CD14 is known to bind LPS and initiate cell...
Lipopolysaccharide smooth-rough phase variation in bacteria of the genus Chlamydia.
Lukácová, M, Baumann, M, Brade, L, Mamat, U, Brade, H
In two strains of Chlamydia psittaci and in Chlamydia trachomatis serotype L1, we have detected a so-far-unknown antigen which (i) is resistant to heat and proteolytic digestion, (ii) can be...
Holst, O, Brade, L, Kosma, P, Brade, H
The human bacterial pathogens Chlamydia spp. possess a genus-specific lipopolysaccharide as a major surface antigen, the structure of which has been determined by analytical chemistry as Kdop alpha...
A phosphorylated 2-keto-3-deoxyoctonic acid (KDO) was released from the lipopolysaccharides of Vibrio cholerae Ogawa and Inaba after strong acid hydrolysis. The phosphorylated KDO was identified by...
Fu, Y, Baumann, M, Kosma, P, Brade, L, Brade, H
The tetrasaccharide 3-deoxy-alpha-D-manno-2-octulosonic acid (alpha-KDO) (2----8)-alpha-KDO(2----4)-alpha-KDO(2----6)-beta GlcNAc, a partial structure of chlamydial lipopolysaccharide (LPS)...
Kuhn, H M, Brade, L, Appelmelk, B J, Kusumoto, S, Rietschel, E T, Brade, H
A series of monoclonal antibodies directed against lipid A was characterized by using synthetic lipid A analogs and partial structures. These compounds vary in phosphate substitution, acylation...
Ulmer, A J, Heine, H, Feist, W, Kusumoto, S, Kusama, T, Brade, H, ...
We investigated the biological activity of four new synthetic analogs of lipid A, termed PE-1, PE-2, PE-3, and PE-4. All compounds contain an alpha-oxyethyl-linked (-O-CH2-CH2-) phosphoryl group in...
Staining of surface antigens of Chlamydia trachomatis L2 in tissue culture.
Baumann, M, Brade, L, Fasske, E, Brade, H
Surface labeling of chlamydial elementary and reticulate bodies in L929 cells infected with Chlamydia trachomatis serotype L2 was monitored by using monoclonal antibodies (MAb) against the major...
Ulmer, A J, Feist, W, Heine, H, Kirikae, T, Kirikae, F, Kusumoto, S, ...
We have previously shown that the synthetic tetraacyl precursor Ia (compound 406, LA-14-PP, or lipid IVa) was not able to induce the production of tumor necrosis factor, interleukin-1, and...
Brade, L, Holst, O, Kosma, P, Zhang, Y X, Paulsen, H, Krausse, R, ...
Murine monoclonal and rabbit, murine, and human polyclonal antibodies against chlamydial lipopolysaccharide (LPS) were characterized by the passive hemolysis and passive hemolysis inhibition assays...
Wang, M H, Flad, H D, Feist, W, Brade, H, Kusumoto, S, Rietschel, E T, ...
The effect of two synthetic lipid A partial structures, compound 406 (or LA-14-PP, identical in structure to the lipid A precursor, known as Ia or IVa) and compound 401 (lipid X), on the in vitro...
Brade, L, Bessler, W G, Brade, H
Synthetic Escherichia coli lipid A and synthetic S-[2,3-bis-(palmitoyloxy)propyl]-N-palmitoylpentapeptide (tripalmitoyl pentapeptide [TPP]), representing the mitogenically active principles of...
Brade, L, Brandenburg, K, Kuhn, H M, Kusumoto, S, Macher, I, Rietschel, E T, ...
We investigated the immunogenicity and antigenicity of synthetic lipid A and partial structures thereof. Included in the study were compounds which varied in the position of phosphate (1-mono-,...
Chemical, biological, and immunochemical properties of the Chlamydia psittaci lipopolysaccharide.
Brade, L, Schramek, S, Schade, U, Brade, H
The lipopolysaccharide (LPS) of Chlamydia psittaci was extracted from yolk sac-grown elementary bodies, purified, and characterized chemically, immunochemically, and biologically. The LPS contained...
Brade, L, Kosma, P, Appelmelk, B J, Paulsen, H, Brade, H
Mouse monoclonal antibodies were raised against heat-killed bacteria of the Re mutant R595 of Salmonella minnesota and characterized by the passive hemolysis and passive hemolysis inhibition tests...
Brade, L, Nano, F E, Schlecht, S, Schramek, S, Brade, H
Rough mutants from Salmonella typhimurium and Salmonella minnesota were transformed with a plasmid containing a 6.5-kilobase insert of DNA from Chlamydia trachomatis assumed to encode a...
Immunogenicity and antigenicity of synthetic Escherichia coli lipid A.
Brade, L, Rietschel, E T, Kusumoto, S, Shiba, T, Brade, H
The immunogenicity and antigenicity of synthetic Escherichia coli lipid A (compound 506) and its 1- and 4'-monophosphorylated derivatives (compounds 505 and 504, respectively) and nonphosphorylated...
Normal mouse serum was found to contain a protein, referred to here as factor, which binds to the inner core region of lipopolysaccharides (LPSs) of various bacterial families. Since factor-LPS...
Antisera were raised in rabbits with acid-treated Re mutant bacteria from Salmonella minnesota and Escherichia coli and tested in a passive hemolysis assay with di- and monophosphorylated free lipid...
Antigenic properties of Chlamydia trachomatis lipopolysaccharide.
Brade, L, Nurminen, M, Mäkelä, P H, Brade, H
The antigenic properties of the lipopolysaccharide (LPS) of Chlamydia trachomatis L2 were investigated. By means of passive hemolysis, passive hemolysis inhibition, and absorption experiments, it was...
Chemical characterization of Chlamydia trachomatis lipopolysaccharide.
Nurminen, M, Rietschel, E T, Brade, H
The group-specific antigen of Chlamydia trachomatis serotype L2 was chemically analyzed. It is composed of typical lipopolysaccharide (LPS) components, i.e., D-glucosamine, long-chain 3-hydroxy fatty...
A new antigenic specificity, referred to here as common lipopolysaccharide (LPS) specificity, is described in the LPSs of gram-negative bacteria belonging to various families. The specificity is...
Serological cross-reactions between Acinetobacter calcoaceticus and chlamydiae.
A cross-reaction between Acinetobacter calcoaceticus and chlamydiae is described. A water-soluble, heat stable, non-dialyzable antigen was extracted from Acinetobacter species by boiling. This...
Swierzko, A, Brade, L, Paulsen, H, Brade, H
Rabbit polyclonal antibodies against the rough mutant lipopolysaccharide (LPS) of Salmonella minnesota R4 (chemotype Rd2P-) were serologically characterized by using R4 LPS, deacylated LPS,...
Di Padova, F E, Brade, H, Barclay, G R, Poxton, I R, Liehl, E, Schuetze, E, ...
During the last decade, episodes of sepsis have increased and Escherichia coli has remained the most frequent clinical isolate. Lipopolysaccharides (LPS; endotoxin) are the major toxic and antigenic...
Monoclonal antibodies (MAbs) against lipid A, the endotoxic component of lipopolysaccharide (LPS) of gram-negative bacteria, are presently discussed as therapeutic agents against lethal gram-negative...
Weidemann, B, Brade, H, Rietschel, E T, Dziarski, R, Bazil, V, Kusumoto, S, ...
We have investigated the interaction of soluble peptidoglycan (sPG), in comparison with lipopolysaccharide (LPS), with human mononuclear cells (MNC) by determining the capacity of sPG to induce...
Members of the bacterial genus Chlamydia are responsible for widespread disease among humans and animals, including endemic trachoma in developing countries, venereal disease in developed countries,...
Rozalski, A, Brade, L, Kosma, P, Appelmelk, B J, Krogmann, C, Brade, H
Murine monoclonal and rabbit polyclonal antibodies raised against the lipopolysaccharides (LPS) of Re mutants of Salmonella minnesota, Proteus mirabilis, and Escherichia coli were serologically...
Schletter, J, Brade, H, Brade, L, Krüger, C, Loppnow, H, Kusumoto, S, ...
Activation of cells by bacterial lipopolysaccharide (LPS) plays a key role in the pathogenesis of gram-negative septic shock. The 55-kDa glycoprotein CD14 is known to bind LPS and initiate cell...
Lipopolysaccharide smooth-rough phase variation in bacteria of the genus Chlamydia.
Lukácová, M, Baumann, M, Brade, L, Mamat, U, Brade, H
In two strains of Chlamydia psittaci and in Chlamydia trachomatis serotype L1, we have detected a so-far-unknown antigen which (i) is resistant to heat and proteolytic digestion, (ii) can be...
Holst, O, Brade, L, Kosma, P, Brade, H
The human bacterial pathogens Chlamydia spp. possess a genus-specific lipopolysaccharide as a major surface antigen, the structure of which has been determined by analytical chemistry as Kdop alpha...
A phosphorylated 2-keto-3-deoxyoctonic acid (KDO) was released from the lipopolysaccharides of Vibrio cholerae Ogawa and Inaba after strong acid hydrolysis. The phosphorylated KDO was identified by...
Fu, Y, Baumann, M, Kosma, P, Brade, L, Brade, H
The tetrasaccharide 3-deoxy-alpha-D-manno-2-octulosonic acid (alpha-KDO) (2----8)-alpha-KDO(2----4)-alpha-KDO(2----6)-beta GlcNAc, a partial structure of chlamydial lipopolysaccharide (LPS)...
Kuhn, H M, Brade, L, Appelmelk, B J, Kusumoto, S, Rietschel, E T, Brade, H
A series of monoclonal antibodies directed against lipid A was characterized by using synthetic lipid A analogs and partial structures. These compounds vary in phosphate substitution, acylation...
Ulmer, A J, Heine, H, Feist, W, Kusumoto, S, Kusama, T, Brade, H, ...
We investigated the biological activity of four new synthetic analogs of lipid A, termed PE-1, PE-2, PE-3, and PE-4. All compounds contain an alpha-oxyethyl-linked (-O-CH2-CH2-) phosphoryl group in...
Staining of surface antigens of Chlamydia trachomatis L2 in tissue culture.
Baumann, M, Brade, L, Fasske, E, Brade, H
Surface labeling of chlamydial elementary and reticulate bodies in L929 cells infected with Chlamydia trachomatis serotype L2 was monitored by using monoclonal antibodies (MAb) against the major...
Ulmer, A J, Feist, W, Heine, H, Kirikae, T, Kirikae, F, Kusumoto, S, ...
We have previously shown that the synthetic tetraacyl precursor Ia (compound 406, LA-14-PP, or lipid IVa) was not able to induce the production of tumor necrosis factor, interleukin-1, and...
Brade, L, Holst, O, Kosma, P, Zhang, Y X, Paulsen, H, Krausse, R, ...
Murine monoclonal and rabbit, murine, and human polyclonal antibodies against chlamydial lipopolysaccharide (LPS) were characterized by the passive hemolysis and passive hemolysis inhibition assays...
Wang, M H, Flad, H D, Feist, W, Brade, H, Kusumoto, S, Rietschel, E T, ...
The effect of two synthetic lipid A partial structures, compound 406 (or LA-14-PP, identical in structure to the lipid A precursor, known as Ia or IVa) and compound 401 (lipid X), on the in vitro...
Brade, L, Bessler, W G, Brade, H
Synthetic Escherichia coli lipid A and synthetic S-[2,3-bis-(palmitoyloxy)propyl]-N-palmitoylpentapeptide (tripalmitoyl pentapeptide [TPP]), representing the mitogenically active principles of...
Brade, L, Brandenburg, K, Kuhn, H M, Kusumoto, S, Macher, I, Rietschel, E T, ...
We investigated the immunogenicity and antigenicity of synthetic lipid A and partial structures thereof. Included in the study were compounds which varied in the position of phosphate (1-mono-,...
Chemical, biological, and immunochemical properties of the Chlamydia psittaci lipopolysaccharide.
Brade, L, Schramek, S, Schade, U, Brade, H
The lipopolysaccharide (LPS) of Chlamydia psittaci was extracted from yolk sac-grown elementary bodies, purified, and characterized chemically, immunochemically, and biologically. The LPS contained...
Brade, L, Kosma, P, Appelmelk, B J, Paulsen, H, Brade, H
Mouse monoclonal antibodies were raised against heat-killed bacteria of the Re mutant R595 of Salmonella minnesota and characterized by the passive hemolysis and passive hemolysis inhibition tests...
Brade, L, Nano, F E, Schlecht, S, Schramek, S, Brade, H
Rough mutants from Salmonella typhimurium and Salmonella minnesota were transformed with a plasmid containing a 6.5-kilobase insert of DNA from Chlamydia trachomatis assumed to encode a...
Immunogenicity and antigenicity of synthetic Escherichia coli lipid A.
Brade, L, Rietschel, E T, Kusumoto, S, Shiba, T, Brade, H
The immunogenicity and antigenicity of synthetic Escherichia coli lipid A (compound 506) and its 1- and 4'-monophosphorylated derivatives (compounds 505 and 504, respectively) and nonphosphorylated...
Normal mouse serum was found to contain a protein, referred to here as factor, which binds to the inner core region of lipopolysaccharides (LPSs) of various bacterial families. Since factor-LPS...
Antisera were raised in rabbits with acid-treated Re mutant bacteria from Salmonella minnesota and Escherichia coli and tested in a passive hemolysis assay with di- and monophosphorylated free lipid...
Antigenic properties of Chlamydia trachomatis lipopolysaccharide.
Brade, L, Nurminen, M, Mäkelä, P H, Brade, H
The antigenic properties of the lipopolysaccharide (LPS) of Chlamydia trachomatis L2 were investigated. By means of passive hemolysis, passive hemolysis inhibition, and absorption experiments, it was...
Chemical characterization of Chlamydia trachomatis lipopolysaccharide.
Nurminen, M, Rietschel, E T, Brade, H
The group-specific antigen of Chlamydia trachomatis serotype L2 was chemically analyzed. It is composed of typical lipopolysaccharide (LPS) components, i.e., D-glucosamine, long-chain 3-hydroxy fatty...
A new antigenic specificity, referred to here as common lipopolysaccharide (LPS) specificity, is described in the LPSs of gram-negative bacteria belonging to various families. The specificity is...
Serological cross-reactions between Acinetobacter calcoaceticus and chlamydiae.
A cross-reaction between Acinetobacter calcoaceticus and chlamydiae is described. A water-soluble, heat stable, non-dialyzable antigen was extracted from Acinetobacter species by boiling. This...
Swierzko, A, Brade, L, Paulsen, H, Brade, H
Rabbit polyclonal antibodies against the rough mutant lipopolysaccharide (LPS) of Salmonella minnesota R4 (chemotype Rd2P-) were serologically characterized by using R4 LPS, deacylated LPS,...
Di Padova, F E, Brade, H, Barclay, G R, Poxton, I R, Liehl, E, Schuetze, E, ...
During the last decade, episodes of sepsis have increased and Escherichia coli has remained the most frequent clinical isolate. Lipopolysaccharides (LPS; endotoxin) are the major toxic and antigenic...
Monoclonal antibodies (MAbs) against lipid A, the endotoxic component of lipopolysaccharide (LPS) of gram-negative bacteria, are presently discussed as therapeutic agents against lethal gram-negative...
Weidemann, B, Brade, H, Rietschel, E T, Dziarski, R, Bazil, V, Kusumoto, S, ...
We have investigated the interaction of soluble peptidoglycan (sPG), in comparison with lipopolysaccharide (LPS), with human mononuclear cells (MNC) by determining the capacity of sPG to induce...
Members of the bacterial genus Chlamydia are responsible for widespread disease among humans and animals, including endemic trachoma in developing countries, venereal disease in developed countries,...
Rozalski, A, Brade, L, Kosma, P, Appelmelk, B J, Krogmann, C, Brade, H
Murine monoclonal and rabbit polyclonal antibodies raised against the lipopolysaccharides (LPS) of Re mutants of Salmonella minnesota, Proteus mirabilis, and Escherichia coli were serologically...