Genomic Islands in the Pathogenic Filamentous Fungus Aspergillus fumigatus (2008)
Fedorova, N.D., Khaldi, N., Joardar, V.S., Maiti, R., Amedeo, P., Anderson, M.J., ...
We present the genome sequences of a new clinical isolate of the important human pathogen, Aspergillus fumigatus, A1163, and two closely related but rarely pathogenic species, Neosartorya fischeri...
An in vitro assay for (1-->6)-beta-D-glucan synthesis in Saccharomyces cerevisiae. (2004)
Vink, E., Rodriguez-Suarez, R.J., Gerard-Vincent, M., Ribas, J.C., Nobel, J.G. De, Ende, H. Van Den, ...
(1 --> 6)-beta-D-glucan is a key cell wall component of Saccharomyces cerevisiae and Candida albicans. Many genes are known to affect the levels or structure of this glucan, but their roles and a...
The nucleotide sequence of chromosome I from Saccharomyces cerevisiae.
Bussey, H, Kaback, D B, Zhong, W, Vo, D T, Clark, M W, Fortin, N, ...
Chromosome I from the yeast Saccharomyces cerevisiae contains a DNA molecule of approximately 231 kbp and is the smallest naturally occurring functional eukaryotic nuclear chromosome so far...
The KRE6 gene product is required for synthesis of the major beta-glucans of the yeast cell wall, as mutations in this gene confer reduced levels of both the (1----6)- and (1----3)-beta-D-glucan...
Martinac, B, Zhu, H, Kubalski, A, Zhou, X L, Culbertson, M, Bussey, H, ...
The patch-clamp technique was used to examine the plasma membranes of sensitive yeast spheroplasts exposed to partially purified killer toxin preparations. Asolectin liposomes in which the toxin was...
Jiang, B, Sheraton, J, Ram, A F, Dijkgraaf, G J, Klis, F M, Bussey, H
CWH41 encodes a novel type II integral membrane N-glycoprotein located in the endoplasmic reticulum. Disruption of the CWH41 gene leads to a K1 killer toxin-resistant phenotype and a 50% reduction in...
Brown, J L, North, S, Bussey, H
A search for genes which, at elevated copy number, could suppress the growth defect in a strain disrupted at the KRE9 locus has identified the SKN7 gene. SKN7 was mapped to the right arm of...
Boone, C, Sdicu, A, Laroche, M, Bussey, H
The KRE1 gene of Saccharomyces cerevisiae, sacKRE1, appears to be involved in the synthesis of cell wall beta-glucan. S. cerevisiae strains with mutations in the KRE1 gene produce a structurally...
Binding of yeast killer toxin to a cell wall receptor on sensitive Saccharomyces cerevisiae.
Bussey, H, Saville, D, Hutchins, K, Palfree, R G
35S-labeled killer toxin protein bound to cells of sensitive Saccharomyces cerevisiae S14a. Strains that were resistant to toxin through mutation in the nuclear genes kre1 kre2 bound toxin only...
Mode of action of yeast toxins: energy requirement for Saccharomyces cerevisiae killer toxin.
The role of the energy status of the yeast cell in the sensitivity of cultures to two yeast toxins was examined by using 12K release from cells as a measure of toxin action. The Saccharomyces...
Membrane-mediated killing of Saccharomyces cerevisiae by glycoproteins from Torulopsis glabrata.
Cell-free supernatants from cultures of Torulopsis glabrata contained glycoprotein toxins that killed sensitive and killer strains of Saccharomyces cerevisiae with single-hit kinetics. Growing S....
Mutational analysis of the functional domains of yeast K1 killer toxin.
To determine the functional domains of K1 killer toxin, we analyzed the phenotypes of a set of mutations throughout regions encoding the alpha- and beta-toxin subunits that allow secretion of mutant...
Roemer, T, Delaney, S, Bussey, H
KRE6 encodes a predicted type II membrane protein which, when disrupted, results in a slowly growing, killer toxin-resistant mutant possessing half the normal level of a structurally wild-type cell...
Meaden, P, Hill, K, Wagner, J, Slipetz, D, Sommer, S S, Bussey, H
Yeast kre mutants define a pathway of cell wall (1----6)-beta-D-glucan synthesis, and mutants in genes KRE5 and KRE6 appear to interact early in such a pathway. We have cloned KRE5, and the sequence...
We have identified and partially characterized the Saccharomyces cerevisiae KEX1 gene product, Kex1p, to assess its role in processing secreted protein precursors. Anti-Kex1p antibodies identified a...
The yeast KRE9 gene encodes an O glycoprotein involved in cell surface beta-glucan assembly.
The yeast KRE9 gene encodes a 30-kDa secretory pathway protein involved in the synthesis of cell wall (1-->6)-beta-glucan. Disruption of KRE9 leads to serious growth impairment and an altered cell...
A full-length cDNA of the M1 double-stranded RNA killer preprotoxin coding region successfully directed the synthesis of secreted K1 toxin when expressed in Saccharomyces cerevisiae from a plasmid...
Yeast killer plasmid mutations affecting toxin secretion and activity and toxin immunity function.
Bussey, H, Sacks, W, Galley, D, Saville, D
M double-stranded RNA (MdsRNA) plasmid mutants were obtained by mutagenesis and screening of a diploid killer culture partially heat cured of the plasmid, so that a high proportion of the cells could...
Secretion of Saccharomyces cerevisiae Killer Toxin: Processing of the Glycosylated Precursor
Bussey, H., Saville, D., Greene, D., Tipper, D. J., Bostian, K. A.
Killer toxin secretion was blocked at the restrictive temperature in Saccharomyces cerevisiae sec mutants with conditional defects in the S. cerevisiae secretory pathway leading to accumulation of...
Yeast Skn7p functions in a eukaryotic two-component regulatory pathway.
Brown, J L, Bussey, H, Stewart, R C
Previous analysis of the amino acid sequence of Skn7p, the product of the yeast SKN7 gene, revealed a potential 'receiver motif' homologous to that found in bacterial response regulators...
Lolle, S, Skipper, N, Bussey, H, Thomas, D Y
Two cDNA clones of the segment of Saccharomyces cerevisiae M1 double-stranded RNA, which codes for the yeast killer toxin, have been expressed in yeast using the expression vector pYT760. Toxin...
Romero, P A, Lussier, M, Sdicu, A M, Bussey, H, Herscovics, A
The yeast genome contains a KRE2/MNT1 family of nine related genes with amino acid similarity to the alpha 1,2-mannosyltransferase Kre2p/Mnt1p, the only member of this family whose enzymic properties...
Hill, K., Boone, C., Goebl, M., Puccia, R., Sdicu, A. M., Bussey, H.
We have cloned, sequenced and disrupted the KRE2 gene of Saccharomyces cerevisiae, identified by killer-resistant mutants with a defective cell wall receptor for the toxin. The KRE2 gene is close to...
Brown, J. L., Kossaczka, Z., Jiang, B., Bussey, H.
Recessive mutations leading to killer resistance identify the KRE9, KRE10 and KRE11 genes. Mutations in both the KRE9 and KRE11 genes lead to reduced levels of (1 -> 6)-β-glucan in the yeast cell...
Lussier, M., White, A. M., Sheraton, J., Di-Paolo, T., Treadwell, J., Southard, S. B., ...
The sequenced yeast genome offers a unique resource for the analysis of eukaryotic cell function and enables genome-wide screens for genes involved in cellular processes. We have identified genes...
Shahinian, S, Dijkgraaf, G J, Sdicu, A M, Thomas, D Y, Jakob, C A, Aebi, M, ...
beta-1,6-Glucan plays a key structural role in the yeast cell wall. Of the genes involved in its biosynthesis, the activity of Cwh41p is known, i.e., the glucosidase I enzyme of protein N-chain...
The nucleotide sequence of chromosome I from Saccharomyces cerevisiae.
Bussey, H, Kaback, D B, Zhong, W, Vo, D T, Clark, M W, Fortin, N, ...
Chromosome I from the yeast Saccharomyces cerevisiae contains a DNA molecule of approximately 231 kbp and is the smallest naturally occurring functional eukaryotic nuclear chromosome so far...
The KRE6 gene product is required for synthesis of the major beta-glucans of the yeast cell wall, as mutations in this gene confer reduced levels of both the (1----6)- and (1----3)-beta-D-glucan...
Martinac, B, Zhu, H, Kubalski, A, Zhou, X L, Culbertson, M, Bussey, H, ...
The patch-clamp technique was used to examine the plasma membranes of sensitive yeast spheroplasts exposed to partially purified killer toxin preparations. Asolectin liposomes in which the toxin was...
Jiang, B, Sheraton, J, Ram, A F, Dijkgraaf, G J, Klis, F M, Bussey, H
CWH41 encodes a novel type II integral membrane N-glycoprotein located in the endoplasmic reticulum. Disruption of the CWH41 gene leads to a K1 killer toxin-resistant phenotype and a 50% reduction in...
Brown, J L, North, S, Bussey, H
A search for genes which, at elevated copy number, could suppress the growth defect in a strain disrupted at the KRE9 locus has identified the SKN7 gene. SKN7 was mapped to the right arm of...
Boone, C, Sdicu, A, Laroche, M, Bussey, H
The KRE1 gene of Saccharomyces cerevisiae, sacKRE1, appears to be involved in the synthesis of cell wall beta-glucan. S. cerevisiae strains with mutations in the KRE1 gene produce a structurally...
Binding of yeast killer toxin to a cell wall receptor on sensitive Saccharomyces cerevisiae.
Bussey, H, Saville, D, Hutchins, K, Palfree, R G
35S-labeled killer toxin protein bound to cells of sensitive Saccharomyces cerevisiae S14a. Strains that were resistant to toxin through mutation in the nuclear genes kre1 kre2 bound toxin only...
Mode of action of yeast toxins: energy requirement for Saccharomyces cerevisiae killer toxin.
The role of the energy status of the yeast cell in the sensitivity of cultures to two yeast toxins was examined by using 12K release from cells as a measure of toxin action. The Saccharomyces...
Membrane-mediated killing of Saccharomyces cerevisiae by glycoproteins from Torulopsis glabrata.
Cell-free supernatants from cultures of Torulopsis glabrata contained glycoprotein toxins that killed sensitive and killer strains of Saccharomyces cerevisiae with single-hit kinetics. Growing S....
Mutational analysis of the functional domains of yeast K1 killer toxin.
To determine the functional domains of K1 killer toxin, we analyzed the phenotypes of a set of mutations throughout regions encoding the alpha- and beta-toxin subunits that allow secretion of mutant...
Roemer, T, Delaney, S, Bussey, H
KRE6 encodes a predicted type II membrane protein which, when disrupted, results in a slowly growing, killer toxin-resistant mutant possessing half the normal level of a structurally wild-type cell...
Meaden, P, Hill, K, Wagner, J, Slipetz, D, Sommer, S S, Bussey, H
Yeast kre mutants define a pathway of cell wall (1----6)-beta-D-glucan synthesis, and mutants in genes KRE5 and KRE6 appear to interact early in such a pathway. We have cloned KRE5, and the sequence...
We have identified and partially characterized the Saccharomyces cerevisiae KEX1 gene product, Kex1p, to assess its role in processing secreted protein precursors. Anti-Kex1p antibodies identified a...
The yeast KRE9 gene encodes an O glycoprotein involved in cell surface beta-glucan assembly.
The yeast KRE9 gene encodes a 30-kDa secretory pathway protein involved in the synthesis of cell wall (1-->6)-beta-glucan. Disruption of KRE9 leads to serious growth impairment and an altered cell...
A full-length cDNA of the M1 double-stranded RNA killer preprotoxin coding region successfully directed the synthesis of secreted K1 toxin when expressed in Saccharomyces cerevisiae from a plasmid...
Yeast killer plasmid mutations affecting toxin secretion and activity and toxin immunity function.
Bussey, H, Sacks, W, Galley, D, Saville, D
M double-stranded RNA (MdsRNA) plasmid mutants were obtained by mutagenesis and screening of a diploid killer culture partially heat cured of the plasmid, so that a high proportion of the cells could...
Secretion of Saccharomyces cerevisiae Killer Toxin: Processing of the Glycosylated Precursor
Bussey, H., Saville, D., Greene, D., Tipper, D. J., Bostian, K. A.
Killer toxin secretion was blocked at the restrictive temperature in Saccharomyces cerevisiae sec mutants with conditional defects in the S. cerevisiae secretory pathway leading to accumulation of...
Yeast Skn7p functions in a eukaryotic two-component regulatory pathway.
Brown, J L, Bussey, H, Stewart, R C
Previous analysis of the amino acid sequence of Skn7p, the product of the yeast SKN7 gene, revealed a potential 'receiver motif' homologous to that found in bacterial response regulators...
Lolle, S, Skipper, N, Bussey, H, Thomas, D Y
Two cDNA clones of the segment of Saccharomyces cerevisiae M1 double-stranded RNA, which codes for the yeast killer toxin, have been expressed in yeast using the expression vector pYT760. Toxin...
Hill, K., Boone, C., Goebl, M., Puccia, R., Sdicu, A. M., Bussey, H.
We have cloned, sequenced and disrupted the KRE2 gene of Saccharomyces cerevisiae, identified by killer-resistant mutants with a defective cell wall receptor for the toxin. The KRE2 gene is close to...
Brown, J. L., Kossaczka, Z., Jiang, B., Bussey, H.
Recessive mutations leading to killer resistance identify the KRE9, KRE10 and KRE11 genes. Mutations in both the KRE9 and KRE11 genes lead to reduced levels of (1 -> 6)-β-glucan in the yeast cell...
Lussier, M., White, A. M., Sheraton, J., Di-Paolo, T., Treadwell, J., Southard, S. B., ...
The sequenced yeast genome offers a unique resource for the analysis of eukaryotic cell function and enables genome-wide screens for genes involved in cellular processes. We have identified genes...
Romero, P A, Lussier, M, Sdicu, A M, Bussey, H, Herscovics, A
The yeast genome contains a KRE2/MNT1 family of nine related genes with amino acid similarity to the alpha 1,2-mannosyltransferase Kre2p/Mnt1p, the only member of this family whose enzymic properties...
Shahinian, S, Dijkgraaf, G J, Sdicu, A M, Thomas, D Y, Jakob, C A, Aebi, M, ...
beta-1,6-Glucan plays a key structural role in the yeast cell wall. Of the genes involved in its biosynthesis, the activity of Cwh41p is known, i.e., the glucosidase I enzyme of protein N-chain...