Scientific Commons: H. C. M. Kester

Endopolygalacturonases from Botrytis cinerea: biochemical properties and interaction with inhibiting proteins (2005)

Krooshof, G.H., Joosten, R., Kester, H.C.M., Kars, I., Benen, J.A.E.

The phytopathogen Botrytis cinerea harbours at least six endopolygalacturonase-encoding (Bcpg) genes of which Bcpg1 and Bcpg2 are required for full virulence. The endopolygalacturonase (BcPG) enzymes...

Endo-polygalacturonases from Botrytis cinerea: biochemical properties and interaction with inhibiting proteins (2004)

Krooshof, G.H., Joosten, R., Kester, H.C.M., Kars, I., Benen, J.A.E.

Endopolygalacturonases from Botrytis cinerea (2004)

Krooshof, G.H., Joosten, R., Kester, H.C.M., Kars, I., Benen, J.A.E.

Endopolygalacturonases van Botrytis cinerea: karakteristieken in vitro (2003)

Krooshof, G.H., Kester, H.C.M., Burgers, K., Benen, J.

Characterisation of endopolygalacturonases from Botrytis cinerea (2003)

Krooshof, G.H., Kester, H.C.M., Kars, I., Benen, J.A.E.

Endopolygalacturonases van Botrytris cinerea: karakteristieken in vitro (2003)

Krooshof, G.H., Kester, H.C.M., Burgers, K., Benen, J.A.E.

The faeB gene encodes a second feruloyl esterase involved in pectin and xylan degradation, and is specifically induced on aromatic compounds (2002)

Kester, H.C.M., Visser, J.

Transglutaminases (Tgases) are a widely distributed group of enzymes that catalyse the post-translational modification of proteins by the formation of isopeptide bonds. This occurs either through...

Use of amide exchange mass spectrometry to study conformational changes within the endopolygalacturonase II-homogalacturonan-polygalacturonase inhibiting protein system (2002)

King, D., Bergmann, C., Orlando, R., Benen, J.A.E., Kester, H.C.M., Visser, J.

Amide exchange mass spectrometry (MS) was used to study the enzyme endopolygalacturonase II (EPG-II) from Aspergillus niger as it binds to an oligosaccharide substrate. A localized decrease in the...

The B1,4- endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions (2002)

Parenicova, L., Hinz, S.W.A., Kester, H.C.M., Benen, J.A.E., Visser, J.

The Aspergillus niger-1,4-endogalactanase encoding gene (galA) was cloned and characterized. The expression of galA in A. niger was only detected in the presence of sugar beet pectin, d-galacturonic...

The ß-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions (2002)

Parenicova, L., Hinz, S.W.A., Kester, H.C.M., Beldman, G., Benen, J.A.E., ...

The Aspergillus nigerß-1,4-endogalactanase encoding gene (galA) was cloned and characterized. The expression of galA in A. niger was only detected in the presence of sugar beet pectin,...

Cloning of a prolidase gene from Aspergillus nidulans and characterisation of its product (2002)

Jalving, R., Bron, P., Kester, H.C.M., Visser, J., Schaap, P.J.

Using EST sequence information available from the filamentous fungus Aspergillus nidulans as a starting point, we have cloned the prolidase-encoding gene, designated pepP. Introduction of multiple...

The Aspergillus niger faeB gene encodes a second feruloyl esterase involved in pectin and xylan degradation and is specifically induced in the presence of aromatic compounds (2002)

VanKuyk, P.A., Kester, H.C.M., Visser, J.

The faeB gene encoding a second feruloyl esterase from Aspergillus niger has been cloned and characterized. It consists of an open reading frame of 1644 bp containing one intron. The gene encodes a...

Changing a single amino acid residue switches processive and non-processive behavior of Aspergillus niger endopolygalacturonase I and II (2001)

Pages, S., Kester, H.C.M., Visser, J., Benen, J.A.E.

Processivity, also known as multiple attack on a single chain, is a feature commonly encountered only in enzymes in which the substrate binds in a tunnel. However, of the seven Aspergillus niger...

Characterization of pectate lyase A from Aspergillus niger (2001)

Benen, J.A.E., Parenicova, L., Kester, H.C.M., Visser, J.

The Aspergillus niger plyA gene encoding pectate lyase A (EC 4.2.99.3) was cloned from a chromosomal EMBL4 library using the Aspergillus nidulans pectate lyase encoding gene [Dean, R. A., and...

The active-site topology of Aspergillus niger endopolygalacturonase II as studied by site-directed mutagenesis (2000)

Armand, S., Wagemaker, M.J.M., Sanchez-Torres, P., Kester, H.C.M., Dijkstra, B.W., ...

Strictly conserved charged residues among polygalacturonases (Asp-180, Asp-201, Asp-202, His-223, Arg-256, and Lys-258) were subjected to site-directed mutagenesis in Aspergillus niger...

Tandem mass spectrometric analysis of Aspergillus niger pectin methylesterase: mode of action on fully methylesterified oligogalacturonates (2000)

Kester, H.C.M., Esteban Warren, M., Orlando, R., Benen, J.A.E., Bergmann, C., Visser, J.

The substrate specificity and the mode of action of Aspergillus niger pectin methylesterase (PME) was determined using both fully methyl-esterified oligogalacturonates with degrees of polymerization...

Subsite Mapping of Aspergillus niger Endopolygalacturonase II by Site-directed Mutagenesis (2000)

Pages, S., Heijne, W.H.M., Kester, H.C.M., Visser, J., Benen, J.A.E.

To assess the subsites involved in substrate binding in Aspergillus niger endopolygalacturonase II, residues located in the potential substrate binding cleft stretching along the enzyme from the N to...

pgaD encodes a new type of endopolygalacturonase from Aspergillus niger (2000)

Parenicova, L., Kester, H.C.M., Benen, J.A.E., Visser, J.

We isolated and characterized a new type of endopolygalacturonase (PG)-encoding gene, pgaD, from Aspergillus niger. The primary structure of PGD differs from that of other A. niger PGs by a 136 amino...

pgaA and pgaB encode constitutively expressed members of the Aspergillus niger endopolygalacturonase gene family (2000)

Parenicova, L., Benen, J.A.E., Kester, H.C.M., Visser, J.

The nucleotide sequence data for pgaA and pgaB have been deposited with the EMBL, GenBank and DDBJ Databases under accession numbers Y18804 and Y18805 respectively. pgaA and pgaB, two genes encoding...

Kinetic characterization of Aspergillus niger N400 endopolygalacturonases I, II and C (1999)

Benen, J.A.E., Kester, H.C.M., Visser, J.

Structure-function relationships in polygalacturonases : a site-directed mutagenesis approach (1999)

Benen, J.A.E., Kester, H.C.M., Armand, S., Sanchez-Torres, P., Parenicová, L., Pagés, S., ...

Performance of selected microbial pectinases on synthetic mono-methylesterified di- and trigalacturonates (1999)

Kester, H.C.M., Magaud, D., Roy, C., Anker, D., Doutheau, A., Shevchik, V., ...

Two monomethyl esters of -(1-4)-linked D-galacturonic dimers and three monomethyl esters of -(1-4)-linked D-galacturonic acid trimers were synthesized chemically and further used as substrates in...

The exopolygalacturonase from Aspergillus tubingensis is also active on xylogalacturonan (1999)

Kester, H.C.M., Benen, J.A.E., Visser, J.

Modes of action of five different endopectate lyases from Erwinia chrystanthemi (1999)

Roy, C., Kester, H.C.M., Visser, J., Shevchik, V.E., Robert-Baudouy, J., ...

Mode of action of five different endopectate lyases from Erwinia chrysanthemi 3937 (1999)

Roy, C., Kester, H.C.M., Benen, J.A.E., Schevchik, V.E., Robert-Baudouy, J., ...

Structure of plant cell wall fragment complexed to pectate lyase C (1999)

Scavetta, R.D., Herron, S.R., Hotchkiss, A.T., Kita, N., Keen, N.T., Benen, J.A.E., ...

Rhamnogalacturonases and polygalacturonases of Aspergillus niger. (1998)

Benen, J.A.E., Kester, H.C.M., Parenicova, L., Visser, J.

pgaE encodes a fourth member of the endopolygalacturonase gene family from Aspergillus niger. (1998)

Parenicov, L., Benen, J.A.E., Kester, H.C.M., Visser, J.

The three-dimensional structure of Aspergillus niger pectin lyase B at 1.7 A resolution. (1998)

Vitali, J., Schick, B., Kester, H.C.M., Visser, J., Jurnak, F.

Cloning and characterization of two rhamnogalacturonan hydrolase genes from Aspergillus niger. (1997)

Suykerbuyk, M.E.G., Kester, H.C.M., Schaap, P.J., Stam, H., Musters, W., Visser, J.

Inversion of configuration during hydrolysis of _-1,4-galacturonidic linkage by three Aspergillus polygalacturonases. (1996)

Biely, P., Benen, J., Heinrichova, K., Kester, H.C.M., Visser, J.

Primary structure and characterization of an exopolygalacturonase from Aspergillus tubingensis. (1996)

Kester, H.C.M., Müller, Y., Visser, J.

Kinetics and mode of action of Aspergillus niger polygalacturonases. (1996)

Benen, J.A.E., Kester, H.C.M., Parenicová, L., Visser, J.

pgaE from Aspergillus niger encodes a fourth polygalacturonase. Molecular cloning and biochemical characterization of the gene product. (1996)

Parenicová, L., Benen, J.A.E., Kester, H.C.M., Visser, J.

Stereochemistry of hydrolysis of glycosidic linkage by three Aspergillus polygalacturonases. (1996)

Biely, P., Benen, J.A.E., Kester, H.C.M., Heinrichova, K., Visser, J.

Molecular genetic and biochemical aspects of pectin degradation in Aspergillus. (1996)

Benen, J.A.E., Parenicová, L., Kester, H.C.M., Visser, J.

Crystallization and Preliminary Crystallographic Characterization of Endo-polygalacturonase II from Aspergillus niger (1994)

Visser, J., Kester, H.C.M., Arkema, A., Dijkstra, B.W.

The endo-polygalacturonase II from Aspergillus niger has been crystallized from an ammonium sulfate solution by the hanging drop method. The crystals belong to the monoclinic space group P21, with...

Crystallization and Preliminary Crystallographic Characterization of Endo-polygalacturonase II from Aspergillus niger (1994)

Dijkstra, B.W., Visser, J., Kester, H.C.M., Arkema, A.

The endo-polygalacturonase II from Aspergillus niger has been crystallized from an ammonium sulfate solution by the hanging drop method. The crystals belong to the monoclinic space group P21, with...

Cloning and expression of the exo-polygalacturonase gene from Aspergillus. (1994)

Müller, Y., Kester, H.C.M., Visser, J.

Purification and characterization of pectin lyase B, a novel pectinolytic enzyme from Aspergillus niger. (1994)

Kester, H.C.M., Visser, J.

Crystallisation and preliminary crystallographic characterization of endopolygalacturonase II from Aspergillus niger. (1994)

Schröter, K.H., Arkema, A., Kester, H.C.M., Visser, J., Dijkstra, B.W.

Identification and characterization of a second polygalacturonase gene of Aspergillus niger. (1991)

Bussink, H.J.D., Brouwer, K.B., Kester, H.C.M., Visser, J.

Structure of the Aspergillus niger pelA gene and its expression in Aspergillus niger and Aspergillus nidulans. (1991)

Harmsen, J.A.M., Kester, H.C.M., Visser, J.

Molecular cloning, nucleotide sequence and expression of the gene encoding prepro-polygalacturonaseII of Aspergillus niger. (1990)

Bussink, H.J.D., Kester, H.C.M., Visser, J.

Isolation and structure of the pectin lyase D encoding gene from Aspergillus niger. (1990)

Gysler, C., Harmsen, J.A.M., Kester, H.C.M., Visser, J., Heim, J.

Purification and characterisation of polygalacturonases produced by the hyphal fungus Aspergillus niger. (1990)

Kester, H.C.M., Visser, J.

Regulation of phosphofructokinase 1 and 2 in relation to acidogenesis in Aspergillus niger. (1990)

Steinböck, F., Harmsen, H., Kester, H.C.M., Kubicek-Pranz, E.M., Rohr, M., Visser, J., ...

Pectin degradation by Aspergillus niger. (1990)

Visser, J., Bussink, H.J.D., Kester, H.C.M.

Fungal expression system. (1990)

Visser, J., Bussink, H.J.D., Kester, H.C.M., Buxton, F.

The invention concerns a recombinant DNA molecule coding for a polygalacturonase (PG) expression cassette comprising the structural gene of a PG and/or corresponding regulatory sequences, or hybrid...

Isolation, structure and expression of a fungal gene coding for pectin lyase. (1989)

Gysler, C., Harmsen, J.A.M., Kester, H.C.M., Visser, J., Heim, J.

Variation in pectinolytic enzymes of the black Aspergilli: a biochemical and genetic approach. (1989)

Kester, H.C.M., Samson, R.A., Visser, J.

Isolation and structure of four members of a pectin lyase gene family in A. niger. (1989)

Visser, J., Harmsen, J., Kester, H.C.M., Flipphi, M., Heim, J., ...

Isolation and structure of the pectin lyase D gene of A. niger. (1989)

Visser, J., Harmsen, J., Kester, H.C.M., Gysler, C., Heim, J., ...

Induktie en karakterisering van enkele door Aspergillus niger aangemaakte arabinases. (1989)

Kester, H.C.M., Voragen, A.G.J., Visser, J.

A rapid purification procedure for pyruvate kinase from the hyphal fungus Aspergillus nidulans. (1988)

Kester, H.C.M., Visser, J.

Polygalacturonases from Aspergillus niger: Purification and properties. (1988)

Kester, H.C.M., Visser, J.

Novel expression system (4-17 145). (1988)

Visser, J., Harmsen, J.A.M., Kester, H.C.M., Heim, J., Hinnen, A.

Novel expression system. (1987)

Heim, J., Gysler, C., Kester, H.C.M., Visser, J.

Recombinant DNA molecules coding for a pectin lyase expression system and derivatives thereof, such as the structural gene of PLI and corresponding regulatory sequences, e.g. promoter, signal and...

Crossed affinity immunoelectrophoresis of the Escherichia coli pyruvate dehydrogenase complex. (1986)

Visser, J., Kester, H.C.M.

Affinity electrophoresis, a general introduction and application of reactive dyes (1985)

Visser, J., Kester, H.C.M.

In vivo NMR studies on carbon metabolism of the hyphal fungus Aspergillus (1985)

Visser, J., Kester, H.C.M., Bos, C.J., Dijkema, C.

In vivo studies on carbon metabolism of the hyphal fungus Aspergillus (1985)

Visser, J., Kester, H.C.M., Bos, C.J., Dijkema, C.

13C NMR studies of carbon metabolism in the hyphal fungus Aspergillus nidulans (1985)

Dijkema, C., Kester, H.C.M., Visser, J.

13C NMR as diagnostic tool for the characterization of enzymatic blocks in mutants of the fungus Aspergillus nidulans (1984)

Dijkema, C., Kester, H.C.M., Visser, J.