Characterization of vancomycin resistance in Enterococcus durans (1995)
Cercenado, E., Ünal, S., Eliopoulos, C. T., Rubin, L. G., Isenberg, H. D., Moellering, R. C., ...
During investigation of an outbreak of vancomycin resistant Enterococcus faecium in a paediatric hospital, an isolate of Enterococcus durans resistant to vancomycin, teicoplanin, ampicillin and...
Susceptibility testing of Propionibacterium acnes comparing agar dilution with E test.
Smith, M A, Alperstein, P, France, K, Vellozzi, E M, Isenberg, H D
Propionibacterium acnes has been identified as a significant agent of nosocomial infections, including endophthalmitis. Data concerning susceptibility of P. acnes to newer beta-lactam antibiotics and...
Isenberg, H D, Alperstein, P, France, K
Ampicillin-sulbactam disks containing either 10 microg of each drug or 20 microg of each drug were tested against 138 recently, sequentially isolated members of the family Enterobacteriaceae. Results...
Use of disinfectants to reduce microbial contamination of hubs of vascular catheters.
Salzman, M B, Isenberg, H D, Rubin, L G
The vascular catheter hub is a potential portal of entry for microorganisms that cause catheter-related sepsis. Thus, a reduction in catheter hub contamination might reduce the incidence of...
Genotypic variation and slime production among blood and catheter isolates of Candida parapsilosis.
Branchini, M L, Pfaller, M A, Rhine-Chalberg, J, Frempong, T, Isenberg, H D
Candida parapsilosis is an important nosocomial pathogen that can proliferate in high concentrations of glucose and form biofilms on prosthetic materials. We investigated the genotypic diversity and...
Clostridium bifermentans bacteremia with metastatic osteomyelitis.
Scanlan, D R, Smith, M A, Isenberg, H D, Engrassia, S, Hilton, E
Osteomyelitis caused solely by an anaerobic organism is uncommon. We report a case of recurrent Clostridium bifermentans bacteremia resulting in metastatic osteomyelitis involving the sacrum, spine,...
Clinical significance of Staphylococcus warneri bacteremia.
Kamath, U, Singer, C, Isenberg, H D
Twenty-seven episodes of bacteremia caused by Staphylococcus warneri were identified at Long Island Jewish Medical Center in New York between 1984 and 1989. Fourteen of these were thought to...
Abscess and empyema caused by Legionella micdadei.
Halberstam, M, Isenberg, H D, Hilton, E
Legionella micdadei is the second most common species implicated in the occurrence of Legionella pneumonia (D. J. Bremer, Semin. Respir. Infect. 4:190-205, 1987). Although there has been a reported...
Nosocomial urinary tract infection with a slowly growing, fastidious Escherichia coli.
Escherichia coli recovered from a nosocomially acquired urinary tract infection required 48 h of incubation on blood agar and did not grow on other routine clinical laboratory media. This bacterium...
Krilov, L R, Marcoux, L, Isenberg, H D
We prospectively evaluated three enzyme immunoassays (EIAs) and a direct fluorescent-antibody (DFA) test for respiratory syncytial virus detection. Of 90 specimens, 79% gave the same results in all...
Clinical laboratory challenges in the recognition of Leuconostoc spp.
Isenberg, H D, Vellozzi, E M, Shapiro, J, Rubin, L G
Vancomycin-resistant Streptococcus salivarius-like bacteria were isolated from the blood and gastrostomy tube of a 6-month-old child during vancomycin therapy for Staphylococcus epidermidis central...
Microbiology of indwelling central intravascular catheters.
Haslett, T M, Isenberg, H D, Hilton, E, Tucci, V, Kay, B G, Vellozzi, E M
Indwelling central-line catheters (n = 502) from 362 patients in intensive care units were analyzed prospectively. The skin site, 6-cm-distal and -proximal subcutaneous segments of the catheter,...
Novel application of video image processing to biochemical and antimicrobial susceptibility testing.
D'Amato, R F, Isenberg, H D, McKinley, G A, Baron, E J, Tepper, R, Shulman, M
ALADIN (Analytab Products, Plainview, N.Y.) is an automated instrument that uses video imaging (computer-assisted guided video camera) for the determination of biochemical and antimicrobial...
Odontogenic infection secondary to Leuconostoc species.
Wenocur, H S, Smith, M A, Vellozzi, E M, Shapiro, J, Isenberg, H D
Leuconostoc species are gaining importance as pathogenic organisms. We present the first case of odontogenic infection caused by Leuconostoc spp. Isolates initially identified as streptococci were...
Single-source outbreak of Candida tropicalis complicating coronary bypass surgery.
Isenberg, H D, Tucci, V, Cintron, F, Singer, C, Weinstein, G S, Tyras, D H
Candida tropicalis was isolated from the sternal wounds of eight coronary bypass patients from 18 to 89 days postoperatively; infections were limited to soft tissue in five patients but involved the...
Clinical laboratory studies of disinfection with Sporicidin.
The clinical microbiology laboratory evaluation of disinfectants can serve as a guide for their application to reduce hospital-acquired infections. The use of Sporicidin, a glutaraldehyde-phenol...
Acid-fast bacilli in sputum: a case of Legionella micdadei pneumonia.
Hilton, E, Freedman, R A, Cintron, F, Isenberg, H D, Singer, C
Legionella micdadei has been implicated as a cause of nosocomial pneumonia. There are no reports of L. micdadei pneumonia diagnosed by acid-fast stain of expectorated sputum. We report a case of L....
Isenberg, H D, Tilton, R C, Barry, A L, Beskid, G, Cleeland, R, Fallat, V, ...
Methodology for the performance of synergistic antibiotic susceptibility studies has not been standardized. We addressed this problem collaboratively with combinations of amdinocillin and select...
Collaborative clinical laboratory evaluation of an individual MIC strip system.
Isenberg, H D, D'Amato, R F, McKinley, G A, Green, L H, Canonica, F P, Fredericks, L M, ...
Susceptibility results obtained with individualized MIC strips (MICRO-MIC) agreed with the standard microdilution broth method at a level of 96% or greater for each of the 10 antimicrobial agents...
Candida zeylanoides: another opportunistic yeast.
Levenson, D, Pfaller, M A, Smith, M A, Hollis, R, Gerarden, T, Tucci, C B, ...
A patient with a long history of scleroderma and gastrointestinal malabsorption requiring total parenteral nutrition was admitted with Candida zeylanoides fungemia. The yeast responded to therapy,...
Isenberg, H D, D'Amato, R F, Heifets, L, Murray, P R, Scardamaglia, M, Jacobs, M C, ...
A study to delineate the feasibility of a biphasic-culture approach for detection and isolation of mycobacteria from clinical specimens except blood was conducted in four medical centers. The...
Evaluation of the Roche Septi-Chek AFB system for recovery of mycobacteria.
D'Amato, R F, Isenberg, H D, Hochstein, L, Mastellone, A J, Alperstein, P
The Septi-Chek AFB system for the recovery of mycobacteria from clinical specimens was compared with a conventional approach using Lowenstein-Jensen and Middlebrook 7H11 agars. A total of 1,532...
Isenberg, H D, Tucci, V, Lipsitz, P, FAcklam, R R
Streptococcus pyogenes, nontypable with available M antisera, T type 12, and reactive in the serum opacity test, produced various lesions in 10 newborn infants during a 2-month period. All infants...
Collaborative evaluation of the UniScept quantitative antimicrobial susceptibility test.
Isenberg, H D, D'Amato, R F, McKinley, G A, Hochstein, L, Sampson-Scherer, J
UniScept (Analytab Products, Plainview, N.Y.) is a commercially prepared microdilution antimicrobial susceptibility system for the determination, in a single tray, of antimicrobial MICs for...
Collaborative evaluation of the UniScept qualitative antimicrobial susceptibility test.
D'Amato, R F, Isenberg, H D, McKinley, G A, Hochstein, L, Cleri, D J
The UniScept system (Analytab Products, Plainview, N.Y.) is a commercially prepared microdilution antimicrobial susceptibility test for the determination of qualitative susceptibility results for...
Restriction fragment analysis of a Candida tropicalis outbreak of sternal wound infections.
Doebbeling, B N, Hollis, R J, Isenberg, H D, Wenzel, R P, Pfaller, M A
An apparent single-source outbreak of Candida tropicalis sternal wound infections in eight patients was investigated by utilizing DNA restriction fragment analysis (RFA) with HindIII and BstNI. All...
Isenberg, H D, Gavan, T L, Sonnenwirth, A, Taylor, W I, Washington, J A
More than 4,000 clinical urine specimens were evaluated with an automated microbial detection/identification system compared to a standarized manual analysis and the routine modalities used in five...
Collaborative investigation of the AutoMicrobic System Enterobacteriaceae biochemical card.
Isenberg, H D, Gavan, T L, Smith, P B, Sonnenwirth, A, Taylor, W, Martin, W J, ...
The Enterobacteriaceae biochemical card used in six separate laboratories to identify 170 representatives of Enterobacteriaceae. The AutoMicrobic System (Vitek Systems, Inc.) performed with an...
Rapid screening for bacteriuria by light scatter photometry (Autobac): a collaborative study.
Hale, D C, Wright, D N, McKie, J E, Isenberg, H D, Jenkins, R D, Matsen, J M
A total of 2,720 urine specimens from three laboratories were evaluated by Autobac (Pfizer Diagnostics) and were compared with simultaneous colony counts for evidence of bacteriuria. Of 599 specimens...
Hospital cluster epidemic with Morganella morganii.
A cluster epidemic of 13 Morganella morganii infections involving 11 patients occurred over a 3-month period in 1977. This epidemic was unusual in that it involved four services and five hospital...
Isenberg, H D, Sampson-Scherer, J
The Oxi-ferm tube, an eight-compartment system suitable for the recognition of nonfermentative, gram-negative, rod-shaped bacteria, was evaluated in conjunction with the oxidase test in a clinical...
Multi-Laboratory Evaluation of an Automated Microbial Detection/Identification System
Smith, P. B., Gavan, T. L., Isenberg, H. D., Sonnenwirth, A., Taylor, W. I., Washington, J. A., ...
An automated and computerized system (Automicrobic System [AMS]) for the detection of frequently encountered bacteria in clinical urine specimens was tested in a collaborative study among six...
The mechanism of action of a single dose of methylprednisolone on acute inflammation in vivo.
Wiener, S L, Wiener, R, Urivetzky, M, Shafer, S, Isenberg, H D, Janov, C, ...
A model system for the study of inflammation in vivo has been developed using the 16-h polyvinyl sponge implant in the rat. This system allows for simultaneous measurement of in vivo chemotaxis,...
Pathogenicity and virulence: another view.
The concepts of pathogenicity and virulence have governed our perception of microbial harmfulness since the time of Pasteur and Koch. These concepts resulted in the recognition and identification of...
Rat polyvinyl sponge model for the study of infections: initial investigations.
Isenberg, H D, Wiener, S L, Isenberg, G A, Sampson-Scherer, J, Urivetzky, M, Berkman, J I
Polyvinyl sponges were implanted subcutaneously on both sides of young female rats. One sponge was infected with 10(8) of either Escherichia coli K-12 F-, Staphylococcus aureus ATCC 25923, or...
Rat polyvinyl sponge model for the study of infections: host factors and microbial proliferation.
Isenberg, H D, Wiener, S L, Isenberg, G A, Sampson-Scherer, J, Urivetzky, M, Berkman, J I
Female rats were treated with several administration regimens of methylprednisolone, cobra venom anti-complementary factor, and cyclophosphamide in conjunction with polyvinyl sponge implantations....
Susceptibility testing of Propionibacterium acnes comparing agar dilution with E test.
Smith, M A, Alperstein, P, France, K, Vellozzi, E M, Isenberg, H D
Propionibacterium acnes has been identified as a significant agent of nosocomial infections, including endophthalmitis. Data concerning susceptibility of P. acnes to newer beta-lactam antibiotics and...
Isenberg, H D, Alperstein, P, France, K
Ampicillin-sulbactam disks containing either 10 microg of each drug or 20 microg of each drug were tested against 138 recently, sequentially isolated members of the family Enterobacteriaceae. Results...
Use of disinfectants to reduce microbial contamination of hubs of vascular catheters.
Salzman, M B, Isenberg, H D, Rubin, L G
The vascular catheter hub is a potential portal of entry for microorganisms that cause catheter-related sepsis. Thus, a reduction in catheter hub contamination might reduce the incidence of...
Genotypic variation and slime production among blood and catheter isolates of Candida parapsilosis.
Branchini, M L, Pfaller, M A, Rhine-Chalberg, J, Frempong, T, Isenberg, H D
Candida parapsilosis is an important nosocomial pathogen that can proliferate in high concentrations of glucose and form biofilms on prosthetic materials. We investigated the genotypic diversity and...
Clostridium bifermentans bacteremia with metastatic osteomyelitis.
Scanlan, D R, Smith, M A, Isenberg, H D, Engrassia, S, Hilton, E
Osteomyelitis caused solely by an anaerobic organism is uncommon. We report a case of recurrent Clostridium bifermentans bacteremia resulting in metastatic osteomyelitis involving the sacrum, spine,...
Clinical significance of Staphylococcus warneri bacteremia.
Kamath, U, Singer, C, Isenberg, H D
Twenty-seven episodes of bacteremia caused by Staphylococcus warneri were identified at Long Island Jewish Medical Center in New York between 1984 and 1989. Fourteen of these were thought to...
Abscess and empyema caused by Legionella micdadei.
Halberstam, M, Isenberg, H D, Hilton, E
Legionella micdadei is the second most common species implicated in the occurrence of Legionella pneumonia (D. J. Bremer, Semin. Respir. Infect. 4:190-205, 1987). Although there has been a reported...
Nosocomial urinary tract infection with a slowly growing, fastidious Escherichia coli.
Escherichia coli recovered from a nosocomially acquired urinary tract infection required 48 h of incubation on blood agar and did not grow on other routine clinical laboratory media. This bacterium...
Krilov, L R, Marcoux, L, Isenberg, H D
We prospectively evaluated three enzyme immunoassays (EIAs) and a direct fluorescent-antibody (DFA) test for respiratory syncytial virus detection. Of 90 specimens, 79% gave the same results in all...
Clinical laboratory challenges in the recognition of Leuconostoc spp.
Isenberg, H D, Vellozzi, E M, Shapiro, J, Rubin, L G
Vancomycin-resistant Streptococcus salivarius-like bacteria were isolated from the blood and gastrostomy tube of a 6-month-old child during vancomycin therapy for Staphylococcus epidermidis central...
Microbiology of indwelling central intravascular catheters.
Haslett, T M, Isenberg, H D, Hilton, E, Tucci, V, Kay, B G, Vellozzi, E M
Indwelling central-line catheters (n = 502) from 362 patients in intensive care units were analyzed prospectively. The skin site, 6-cm-distal and -proximal subcutaneous segments of the catheter,...
Novel application of video image processing to biochemical and antimicrobial susceptibility testing.
D'Amato, R F, Isenberg, H D, McKinley, G A, Baron, E J, Tepper, R, Shulman, M
ALADIN (Analytab Products, Plainview, N.Y.) is an automated instrument that uses video imaging (computer-assisted guided video camera) for the determination of biochemical and antimicrobial...
Odontogenic infection secondary to Leuconostoc species.
Wenocur, H S, Smith, M A, Vellozzi, E M, Shapiro, J, Isenberg, H D
Leuconostoc species are gaining importance as pathogenic organisms. We present the first case of odontogenic infection caused by Leuconostoc spp. Isolates initially identified as streptococci were...
Single-source outbreak of Candida tropicalis complicating coronary bypass surgery.
Isenberg, H D, Tucci, V, Cintron, F, Singer, C, Weinstein, G S, Tyras, D H
Candida tropicalis was isolated from the sternal wounds of eight coronary bypass patients from 18 to 89 days postoperatively; infections were limited to soft tissue in five patients but involved the...
Clinical laboratory studies of disinfection with Sporicidin.
The clinical microbiology laboratory evaluation of disinfectants can serve as a guide for their application to reduce hospital-acquired infections. The use of Sporicidin, a glutaraldehyde-phenol...
Acid-fast bacilli in sputum: a case of Legionella micdadei pneumonia.
Hilton, E, Freedman, R A, Cintron, F, Isenberg, H D, Singer, C
Legionella micdadei has been implicated as a cause of nosocomial pneumonia. There are no reports of L. micdadei pneumonia diagnosed by acid-fast stain of expectorated sputum. We report a case of L....
Isenberg, H D, Tilton, R C, Barry, A L, Beskid, G, Cleeland, R, Fallat, V, ...
Methodology for the performance of synergistic antibiotic susceptibility studies has not been standardized. We addressed this problem collaboratively with combinations of amdinocillin and select...
Collaborative clinical laboratory evaluation of an individual MIC strip system.
Isenberg, H D, D'Amato, R F, McKinley, G A, Green, L H, Canonica, F P, Fredericks, L M, ...
Susceptibility results obtained with individualized MIC strips (MICRO-MIC) agreed with the standard microdilution broth method at a level of 96% or greater for each of the 10 antimicrobial agents...
Candida zeylanoides: another opportunistic yeast.
Levenson, D, Pfaller, M A, Smith, M A, Hollis, R, Gerarden, T, Tucci, C B, ...
A patient with a long history of scleroderma and gastrointestinal malabsorption requiring total parenteral nutrition was admitted with Candida zeylanoides fungemia. The yeast responded to therapy,...
Isenberg, H D, D'Amato, R F, Heifets, L, Murray, P R, Scardamaglia, M, Jacobs, M C, ...
A study to delineate the feasibility of a biphasic-culture approach for detection and isolation of mycobacteria from clinical specimens except blood was conducted in four medical centers. The...
Evaluation of the Roche Septi-Chek AFB system for recovery of mycobacteria.
D'Amato, R F, Isenberg, H D, Hochstein, L, Mastellone, A J, Alperstein, P
The Septi-Chek AFB system for the recovery of mycobacteria from clinical specimens was compared with a conventional approach using Lowenstein-Jensen and Middlebrook 7H11 agars. A total of 1,532...
Isenberg, H D, Tucci, V, Lipsitz, P, FAcklam, R R
Streptococcus pyogenes, nontypable with available M antisera, T type 12, and reactive in the serum opacity test, produced various lesions in 10 newborn infants during a 2-month period. All infants...
Collaborative evaluation of the UniScept quantitative antimicrobial susceptibility test.
Isenberg, H D, D'Amato, R F, McKinley, G A, Hochstein, L, Sampson-Scherer, J
UniScept (Analytab Products, Plainview, N.Y.) is a commercially prepared microdilution antimicrobial susceptibility system for the determination, in a single tray, of antimicrobial MICs for...
Collaborative evaluation of the UniScept qualitative antimicrobial susceptibility test.
D'Amato, R F, Isenberg, H D, McKinley, G A, Hochstein, L, Cleri, D J
The UniScept system (Analytab Products, Plainview, N.Y.) is a commercially prepared microdilution antimicrobial susceptibility test for the determination of qualitative susceptibility results for...
Restriction fragment analysis of a Candida tropicalis outbreak of sternal wound infections.
Doebbeling, B N, Hollis, R J, Isenberg, H D, Wenzel, R P, Pfaller, M A
An apparent single-source outbreak of Candida tropicalis sternal wound infections in eight patients was investigated by utilizing DNA restriction fragment analysis (RFA) with HindIII and BstNI. All...
Isenberg, H D, Gavan, T L, Sonnenwirth, A, Taylor, W I, Washington, J A
More than 4,000 clinical urine specimens were evaluated with an automated microbial detection/identification system compared to a standarized manual analysis and the routine modalities used in five...
Collaborative investigation of the AutoMicrobic System Enterobacteriaceae biochemical card.
Isenberg, H D, Gavan, T L, Smith, P B, Sonnenwirth, A, Taylor, W, Martin, W J, ...
The Enterobacteriaceae biochemical card used in six separate laboratories to identify 170 representatives of Enterobacteriaceae. The AutoMicrobic System (Vitek Systems, Inc.) performed with an...
Rapid screening for bacteriuria by light scatter photometry (Autobac): a collaborative study.
Hale, D C, Wright, D N, McKie, J E, Isenberg, H D, Jenkins, R D, Matsen, J M
A total of 2,720 urine specimens from three laboratories were evaluated by Autobac (Pfizer Diagnostics) and were compared with simultaneous colony counts for evidence of bacteriuria. Of 599 specimens...
Hospital cluster epidemic with Morganella morganii.
A cluster epidemic of 13 Morganella morganii infections involving 11 patients occurred over a 3-month period in 1977. This epidemic was unusual in that it involved four services and five hospital...
Isenberg, H D, Sampson-Scherer, J
The Oxi-ferm tube, an eight-compartment system suitable for the recognition of nonfermentative, gram-negative, rod-shaped bacteria, was evaluated in conjunction with the oxidase test in a clinical...
Multi-Laboratory Evaluation of an Automated Microbial Detection/Identification System
Smith, P. B., Gavan, T. L., Isenberg, H. D., Sonnenwirth, A., Taylor, W. I., Washington, J. A., ...
An automated and computerized system (Automicrobic System [AMS]) for the detection of frequently encountered bacteria in clinical urine specimens was tested in a collaborative study among six...
The mechanism of action of a single dose of methylprednisolone on acute inflammation in vivo.
Wiener, S L, Wiener, R, Urivetzky, M, Shafer, S, Isenberg, H D, Janov, C, ...
A model system for the study of inflammation in vivo has been developed using the 16-h polyvinyl sponge implant in the rat. This system allows for simultaneous measurement of in vivo chemotaxis,...
Pathogenicity and virulence: another view.
The concepts of pathogenicity and virulence have governed our perception of microbial harmfulness since the time of Pasteur and Koch. These concepts resulted in the recognition and identification of...
Rat polyvinyl sponge model for the study of infections: initial investigations.
Isenberg, H D, Wiener, S L, Isenberg, G A, Sampson-Scherer, J, Urivetzky, M, Berkman, J I
Polyvinyl sponges were implanted subcutaneously on both sides of young female rats. One sponge was infected with 10(8) of either Escherichia coli K-12 F-, Staphylococcus aureus ATCC 25923, or...
Rat polyvinyl sponge model for the study of infections: host factors and microbial proliferation.
Isenberg, H D, Wiener, S L, Isenberg, G A, Sampson-Scherer, J, Urivetzky, M, Berkman, J I
Female rats were treated with several administration regimens of methylprednisolone, cobra venom anti-complementary factor, and cyclophosphamide in conjunction with polyvinyl sponge implantations....