Pausing of mammalian cells by cold exposure, limits and opportunities (2003)
Hunt, L., Hacker, D.L, El Abridi, H., Grosjean, H., Jordan, M., De Jesus, M., ...
Ansmant, I., Massenet, S., Grosjean, H., Motorin, Y., Branlant, C.
So far, four RNA:pseudouridine (Ψ)-synthases have been identified in yeast Saccharomyces cerevisiae. Together, they act on cytoplasmic and mitochondrial tRNAs, U2 snRNA and rRNAs from cytoplasmic...
The yeast tRNA:pseudouridine synthase Pus1p displays a multisite substrate specificity (1998)
Motorin, Y., Keith, G., Simon, C., Foiret, D., Simos, G., Hurt, E., ...
A novel enzymatic pathway leading to 1-methylinosine modification in Haloferax volcanii tRNA (1995)
Grosjean, H., Constantinesco, F., Foiret, D., Benachenhou, N.
Transfer RNAs of the extreme halophile Haloferax volcanii contain several modified nucleosldes, among them 1-methylpseudouridine (m1ψ), pseudouridlne (ψ), 2′-0-methylcytosine (Cm) and...
Labuda, D., Striker, G., Grosjean, H., Porschke, D.
The binding of yeast tRNAPha to UUCA, UUCC, UUCCC, UUCUUCU, U4 U5 U6 and U7 was analysed by fluorescence temperature jump and equilibrium sedimentation measurements. In all cases the two observed...
Grosjean, H., Nicoghosian, K., Haumont, E., Söll, D., Cedergren, R.
We have determined the nucleotide sequence of the major species of E.colitRNASer and of a minor species having the same GGA anticodon. These two tRNAs should recognize the UCC and UCU codons, the...
Carbon, P., Haumont, E., Henau, S.De, Keith, G., Grosjean, H.
A combination of several enzymes, RNase-T., nuclease S., T4-poly-nucleotide kinaee and T4-RNA ligase were used to prepare and modify different fragments of feast tRNAAsP (normal anticodon G U C)....
Usage of the three tennination codons in a single eukaryotic cell, the Xenopus laevis oocyte (1981)
Bienz, M., Kubli, E., Kohli, J., DeHenau, S., Huez, G., Marbaix, G., ...
Oocytes from Xenopus laevis were injected with purified amber (UAG), ochre (UAA), and opal (UGA) suppressor tRNAs from yeasts. The radioactively labeled proteins translated from the endogeneous mRNAs...
Ansmant, I., Massenet, S., Grosjean, H., Motorin, Y., Branlant, C.
So far, four RNA:pseudouridine (Ψ)-synthases have been identified in yeast Saccharomyces cerevisiae. Together, they act on cytoplasmic and mitochondrial tRNAs, U2 snRNA and rRNAs from cytoplasmic...
Jiang, H Q, Motorin, Y, Jin, Y X, Grosjean, H
Cell-free yeast extract has been successfully used to catalyze the enzymatic formation of 11 out of the 14 naturally occurring modified nucleotides in yeast tRNAPhe(anticodon GAA). They are m2G10,...
Becker, H F, Motorin, Y, Planta, R J, Grosjean, H
The protein products of two yeast Saccharomyces cerevisiae genes (YNL292w and CBF5) display a remarkable sequence homology with Escherichia coli tRNA:pseudouridine-55 synthase (encoded by gene truB)....
Constantinesco, F, Benachenhou, N, Motorin, Y, Grosjean, H
The structural gene pfTRM1 (GenBank accession no. AF051912), encoding tRNA(guanine-26, N 2- N 2) methyltransferase (EC 2.1.1.32) of the strictly anaerobic hyperthermophilic archaeon Pyrococcus...
Pseudouridine and ribothymidine formation in the tRNA-like domain of turnip yellow mosaic virus RNA.
Becker, H F, Motorin, Y, Florentz, C, Giegé, R, Grosjean, H
The last 82 nucleotides of the 6.3 kb genomic RNA of plant turnip yellow mosaic virus (TYMV), the so-called 'tRNA-like' domain, presents functional, structural and primary sequence homologies with...
Constantinesco, F, Motorin, Y, Grosjean, H
The modification patterns of in vitro transcripts of two yeast Saccharomyces cerevisiae tRNAs (tRNAPheand tRNAAsp) and one archaeal Haloferax volcanii tRNA (tRNAIle) were investigated in the...
A novel enzymatic pathway leading to 1-methylinosine modification in Haloferax volcanii tRNA.
Grosjean, H, Constantinesco, F, Foiret, D, Benachenhou, N
Transfer RNAs of the extreme halophile Haloferax volcanii contain several modified nucleosides, among them 1-methylpseudouridine (m1 psi), pseudouridine (psi), 2'-0-methylcytosine (Cm) and...
Pleiotrophic effects of point mutations in yeast tRNA(Asp) on the base modification pattern.
Edqvist, J, Stråby, K B, Grosjean, H
The base-modification pattern has been studied in several synthetic variants of yeast tRNA(Asp) injected into Xenopus laevis oocytes. Certain point mutations in the D-stem and the variable loop of...
Modified nucleotides in Bacillus subtilis tRNA(Trp) hyperexpressed in Escherichia coli.
Xue, H, Glasser, A L, Desgres, J, Grosjean, H
In the present study, modified nucleotides in the B. subtilis tRNA(Trp) cloned and hyperexpressed in E. coli have been identified by TLC and HPLC analyses. The modification patterns of the two...
Haumont, E, Fournier, M, De Henau, S, Grosjean, H
We have investigated the specificity of the tRNA modifying enzyme that transforms the adenosine at position 34 (wobble position) into inosine in the anticodon of several tRNAs. For this purpose, we...
Carbon, P, Haumont, E, De Henau, S, Keith, G, Grosjean, H
A combination of several enzymes, RNase-T1, nuclease S1, T4-polynucleotide kinase and T4-RNA ligase were used to prepare and modify different fragments of yeast tRNAAsp (normal anticodon G U C). This...
Grosjean, H, Nicoghosian, K, Haumont, E, Söll, D, Cedergren, R
We have determined the nucleotide sequence of the major species of E. coli tRNASer and of a minor species having the same GGA anticodon. These two tRNAs should recognize the UCC and UCU codons, the...
Nonsense suppression in eukaryotes: the use of the Xenopus oocyte as an in vivo assay system.
Bienz, M, Kubli, E, Kohli, J, De Henau, S, Grosjean, H
Amber, ochre, and opal nonsense suppressor tRNAs isolated from yeast were injected into Xenopus laevis oocytes together with purified mRNAs (globin mRNA from rabbit, tobacco mosaic virus-RNA). Yeast...
Fournier, M, Haumont, E, De Henau, S, Gangloff, J, Grosjean, H
An enzymatic procedure for the replacement of the ICG anticodon of yeast tRNAArgII by NCG trinucleotide (N = A, C, G or U) is described. Partial digestion with S1-nuclease and T1-RNAase provides...
Usage of the three termination codons in a single eukaryotic cell, the Xenopus laevis oocyte.
Bienz, M, Kubli, E, Kohli, J, DeHenau, S, Huez, G, Marbaix, G, ...
Oocytes from Xenopus laevis were injected with purified amber (UAG), ochre (UAA), and opal (UGA) suppressor tRNAs from yeasts. The radioactively labeled proteins translated from the endogenous mRNAs...
Identity elements for N2-dimethylation of guanosine-26 in yeast tRNAs.
Edqvist, J, Grosjean, H, Stråby, K B
N2,N2-dimethylguanosine (m2(2)G) is a characteristic nucleoside that is found in the bend between the dihydro-uridine (D) stem and the anticodon (AC) stem in over 80% of the eukaryotic tRNA species...
Mechanism of codon recognition by transfer RNA studied with oligonucleotides larger than triplets.
Labuda, D, Striker, G, Grosjean, H, Porschke, D
The binding of yeast tRNAPhe to UUCA, UUCC, UUCCC, UUCUUCU, U4, U5, U6 and U7 was analysed by fluorescence temperature jump and equilibrium sedimentation measurements. In all cases the two observed...
Convergence and minimal mutation criteria for evaluating early events in tRNA evolution.
Cedergren, R J, LaRue, B, Sankoff, D, Lapalme, G, Grosjean, H
The convergence of ancestral sequences independently constructed from different branches of a phylogenetic tree can be used as a test of homology of data sequences. This criterion has shown that all...
A method for the isolation of specific tRNA precursors.
Vögeli, G, Grosjean, H, Söll, D
tRNA affinity chromatography, based on complex formation between tRNAs with complementary anticodons, has been applied to the isolation of specific tRNA precursors. When [32P]RNA, isolated from an...
Szweykowska-Kulinska, Z, Senger, B, Keith, G, Fasiolo, F, Grosjean, H
We have isolated and sequenced the minor species of tRNA(Ile) from Saccharomyces cerevisiae. This tRNA contains two unusual pseudouridines (psi s) in the first and third positions of the anticodon....
Nuclear pore proteins are involved in the biogenesis of functional tRNA.
Simos, G, Tekotte, H, Grosjean, H, Segref, A, Sharma, K, Tollervey, D, ...
Los1p and Pus1p, which are involved in tRNA biogenesis, were found in a genetic screen for components interacting with the nuclear pore protein Nsp1p. LOS1, PUS1 and NSP1 interact functionally, since...
N1-Methylguanosine (m1G) or wye nucleoside (Y) are found 3' adjacent to the anticodon (position 37) of eukaryotic tRNAPhe. The biosynthesis of these two modified nucleosides has been investigated....
Queuosine modification of the wobble base in tRNAHis influences 'in vivo' decoding properties.
Meier, F, Suter, B, Grosjean, H, Keith, G, Kubli, E
The 'in vivo' decoding properties of four tRNAHis isoacceptors, two from Drosophila melanogaster and two from brewer's yeast, were studied after their microinjection, along with turnip yellow mosaic...
Carbon, P, Haumont, E, Fournier, M, De Henau, S, Grosjean, H
We have investigated the specificity of the enzymes Q-insertase and mannosyl-Q transferase that replace the guanosine at position 34 (wobble base) in the anticodon of several tRNAs by Q or mannosyl-Q...
Droogmans, L, Haumont, E, De Henau, S, Grosjean, H
We have investigated the specificity of the enzyme tRNA (wobble guanosine 2'-O-)methyltransferase which catalyses the maturation of guanosine-34 of eukaryotic tRNAPhe to the 2'-O-methyl derivative...
Gerber, A, Grosjean, H, Melcher, T, Keller, W
We have identified an RNA-specific adenosine deaminase (termed Tad1p/scADAT1) from Saccharomyces cerevisiae that selectively converts adenosine at position 37 of eukaryotic tRNAAla to inosine. The...
Motorin, Y, Bec, G, Tewari, R, Grosjean, H
To elucidate the sequence elements required in the anticodon stem for the recognition of Escherichia coli tRNA(Ser) (GGA) by the E. coli isopentenyl-tRNA:A37 transferase (IPTT), which result in the...
Morin, A, Auxilien, S, Senger, B, Tewari, R, Grosjean, H
We have investigated the specificity of the eukaryotic enzymatic machinery that transforms adenosine at position 37 (3' adjacent to anticodon) of several tRNAs into threonylcarbamoyladenosine...
The yeast tRNA:pseudouridine synthase Pus1p displays a multisite substrate specificity.
Motorin, Y, Keith, G, Simon, C, Foiret, D, Simos, G, Hurt, E, ...
We have previously shown that the yeast gene PUS1 codes for a tRNA:pseudouridine synthase and that recombinant Pus1p catalyzes, in an intron-dependent way, the formation of psi34 and psi36 in the...
Becker, H F, Corda, Y, Mathews, M B, Fourrey, J L, Grosjean, H
Sera of some patients afflicted with the inflammatory disease myositis contain antibodies of the anti-PL-12 type. A fraction of these polyclonal autoantibodies specifically precipitates the fully...
Several genes encoding putative RNA:5-methylcytidine-transferases (m5C-transferases) from different organisms, including yeast, have been identified by sequence homology with the recently identified...
Ansmant, I., Massenet, S., Grosjean, H., Motorin, Y., Branlant, C.
So far, four RNA:pseudouridine (Ψ)-synthases have been identified in yeast Saccharomyces cerevisiae. Together, they act on cytoplasmic and mitochondrial tRNAs, U2 snRNA and rRNAs from cytoplasmic...
Jiang, H Q, Motorin, Y, Jin, Y X, Grosjean, H
Cell-free yeast extract has been successfully used to catalyze the enzymatic formation of 11 out of the 14 naturally occurring modified nucleotides in yeast tRNAPhe(anticodon GAA). They are m2G10,...
Becker, H F, Motorin, Y, Planta, R J, Grosjean, H
The protein products of two yeast Saccharomyces cerevisiae genes (YNL292w and CBF5) display a remarkable sequence homology with Escherichia coli tRNA:pseudouridine-55 synthase (encoded by gene truB)....
Constantinesco, F, Benachenhou, N, Motorin, Y, Grosjean, H
The structural gene pfTRM1 (GenBank accession no. AF051912), encoding tRNA(guanine-26, N 2- N 2) methyltransferase (EC 2.1.1.32) of the strictly anaerobic hyperthermophilic archaeon Pyrococcus...
Pseudouridine and ribothymidine formation in the tRNA-like domain of turnip yellow mosaic virus RNA.
Becker, H F, Motorin, Y, Florentz, C, Giegé, R, Grosjean, H
The last 82 nucleotides of the 6.3 kb genomic RNA of plant turnip yellow mosaic virus (TYMV), the so-called 'tRNA-like' domain, presents functional, structural and primary sequence homologies with...
Constantinesco, F, Motorin, Y, Grosjean, H
The modification patterns of in vitro transcripts of two yeast Saccharomyces cerevisiae tRNAs (tRNAPheand tRNAAsp) and one archaeal Haloferax volcanii tRNA (tRNAIle) were investigated in the...
A novel enzymatic pathway leading to 1-methylinosine modification in Haloferax volcanii tRNA.
Grosjean, H, Constantinesco, F, Foiret, D, Benachenhou, N
Transfer RNAs of the extreme halophile Haloferax volcanii contain several modified nucleosides, among them 1-methylpseudouridine (m1 psi), pseudouridine (psi), 2'-0-methylcytosine (Cm) and...
Pleiotrophic effects of point mutations in yeast tRNA(Asp) on the base modification pattern.
Edqvist, J, Stråby, K B, Grosjean, H
The base-modification pattern has been studied in several synthetic variants of yeast tRNA(Asp) injected into Xenopus laevis oocytes. Certain point mutations in the D-stem and the variable loop of...
Modified nucleotides in Bacillus subtilis tRNA(Trp) hyperexpressed in Escherichia coli.
Xue, H, Glasser, A L, Desgres, J, Grosjean, H
In the present study, modified nucleotides in the B. subtilis tRNA(Trp) cloned and hyperexpressed in E. coli have been identified by TLC and HPLC analyses. The modification patterns of the two...
Haumont, E, Fournier, M, De Henau, S, Grosjean, H
We have investigated the specificity of the tRNA modifying enzyme that transforms the adenosine at position 34 (wobble position) into inosine in the anticodon of several tRNAs. For this purpose, we...
Carbon, P, Haumont, E, De Henau, S, Keith, G, Grosjean, H
A combination of several enzymes, RNase-T1, nuclease S1, T4-polynucleotide kinase and T4-RNA ligase were used to prepare and modify different fragments of yeast tRNAAsp (normal anticodon G U C). This...
Grosjean, H, Nicoghosian, K, Haumont, E, Söll, D, Cedergren, R
We have determined the nucleotide sequence of the major species of E. coli tRNASer and of a minor species having the same GGA anticodon. These two tRNAs should recognize the UCC and UCU codons, the...
Nonsense suppression in eukaryotes: the use of the Xenopus oocyte as an in vivo assay system.
Bienz, M, Kubli, E, Kohli, J, De Henau, S, Grosjean, H
Amber, ochre, and opal nonsense suppressor tRNAs isolated from yeast were injected into Xenopus laevis oocytes together with purified mRNAs (globin mRNA from rabbit, tobacco mosaic virus-RNA). Yeast...
Fournier, M, Haumont, E, De Henau, S, Gangloff, J, Grosjean, H
An enzymatic procedure for the replacement of the ICG anticodon of yeast tRNAArgII by NCG trinucleotide (N = A, C, G or U) is described. Partial digestion with S1-nuclease and T1-RNAase provides...
Usage of the three termination codons in a single eukaryotic cell, the Xenopus laevis oocyte.
Bienz, M, Kubli, E, Kohli, J, DeHenau, S, Huez, G, Marbaix, G, ...
Oocytes from Xenopus laevis were injected with purified amber (UAG), ochre (UAA), and opal (UGA) suppressor tRNAs from yeasts. The radioactively labeled proteins translated from the endogenous mRNAs...
Identity elements for N2-dimethylation of guanosine-26 in yeast tRNAs.
Edqvist, J, Grosjean, H, Stråby, K B
N2,N2-dimethylguanosine (m2(2)G) is a characteristic nucleoside that is found in the bend between the dihydro-uridine (D) stem and the anticodon (AC) stem in over 80% of the eukaryotic tRNA species...
Mechanism of codon recognition by transfer RNA studied with oligonucleotides larger than triplets.
Labuda, D, Striker, G, Grosjean, H, Porschke, D
The binding of yeast tRNAPhe to UUCA, UUCC, UUCCC, UUCUUCU, U4, U5, U6 and U7 was analysed by fluorescence temperature jump and equilibrium sedimentation measurements. In all cases the two observed...
Convergence and minimal mutation criteria for evaluating early events in tRNA evolution.
Cedergren, R J, LaRue, B, Sankoff, D, Lapalme, G, Grosjean, H
The convergence of ancestral sequences independently constructed from different branches of a phylogenetic tree can be used as a test of homology of data sequences. This criterion has shown that all...
A method for the isolation of specific tRNA precursors.
Vögeli, G, Grosjean, H, Söll, D
tRNA affinity chromatography, based on complex formation between tRNAs with complementary anticodons, has been applied to the isolation of specific tRNA precursors. When [32P]RNA, isolated from an...
Szweykowska-Kulinska, Z, Senger, B, Keith, G, Fasiolo, F, Grosjean, H
We have isolated and sequenced the minor species of tRNA(Ile) from Saccharomyces cerevisiae. This tRNA contains two unusual pseudouridines (psi s) in the first and third positions of the anticodon....
Nuclear pore proteins are involved in the biogenesis of functional tRNA.
Simos, G, Tekotte, H, Grosjean, H, Segref, A, Sharma, K, Tollervey, D, ...
Los1p and Pus1p, which are involved in tRNA biogenesis, were found in a genetic screen for components interacting with the nuclear pore protein Nsp1p. LOS1, PUS1 and NSP1 interact functionally, since...
N1-Methylguanosine (m1G) or wye nucleoside (Y) are found 3' adjacent to the anticodon (position 37) of eukaryotic tRNAPhe. The biosynthesis of these two modified nucleosides has been investigated....
Queuosine modification of the wobble base in tRNAHis influences 'in vivo' decoding properties.
Meier, F, Suter, B, Grosjean, H, Keith, G, Kubli, E
The 'in vivo' decoding properties of four tRNAHis isoacceptors, two from Drosophila melanogaster and two from brewer's yeast, were studied after their microinjection, along with turnip yellow mosaic...
Carbon, P, Haumont, E, Fournier, M, De Henau, S, Grosjean, H
We have investigated the specificity of the enzymes Q-insertase and mannosyl-Q transferase that replace the guanosine at position 34 (wobble base) in the anticodon of several tRNAs by Q or mannosyl-Q...
Droogmans, L, Haumont, E, De Henau, S, Grosjean, H
We have investigated the specificity of the enzyme tRNA (wobble guanosine 2'-O-)methyltransferase which catalyses the maturation of guanosine-34 of eukaryotic tRNAPhe to the 2'-O-methyl derivative...
Gerber, A, Grosjean, H, Melcher, T, Keller, W
We have identified an RNA-specific adenosine deaminase (termed Tad1p/scADAT1) from Saccharomyces cerevisiae that selectively converts adenosine at position 37 of eukaryotic tRNAAla to inosine. The...
Motorin, Y, Bec, G, Tewari, R, Grosjean, H
To elucidate the sequence elements required in the anticodon stem for the recognition of Escherichia coli tRNA(Ser) (GGA) by the E. coli isopentenyl-tRNA:A37 transferase (IPTT), which result in the...
Morin, A, Auxilien, S, Senger, B, Tewari, R, Grosjean, H
We have investigated the specificity of the eukaryotic enzymatic machinery that transforms adenosine at position 37 (3' adjacent to anticodon) of several tRNAs into threonylcarbamoyladenosine...
The yeast tRNA:pseudouridine synthase Pus1p displays a multisite substrate specificity.
Motorin, Y, Keith, G, Simon, C, Foiret, D, Simos, G, Hurt, E, ...
We have previously shown that the yeast gene PUS1 codes for a tRNA:pseudouridine synthase and that recombinant Pus1p catalyzes, in an intron-dependent way, the formation of psi34 and psi36 in the...
Becker, H F, Corda, Y, Mathews, M B, Fourrey, J L, Grosjean, H
Sera of some patients afflicted with the inflammatory disease myositis contain antibodies of the anti-PL-12 type. A fraction of these polyclonal autoantibodies specifically precipitates the fully...
Several genes encoding putative RNA:5-methylcytidine-transferases (m5C-transferases) from different organisms, including yeast, have been identified by sequence homology with the recently identified...