Fibronectin Adsorption to Nanopatterned Silicon Surfaces (2008)
I. Salakhutdinov, P. VandeVord, O. Palyvoda, H. Matthew, G. Tatagiri, H. Handa, ...
The possibility of using surface topography for guidance of different biological molecules and cells is a relevant topic that can be applied to a wide research activity. This study investigated the...
Fibronectin Adsorption to Nanopatterned Silicon Surfaces (2008)
I. Salakhutdinov, P. VandeVord, O. Palyvoda, H. Matthew, G. Tatagiri, H. Handa, ...
The possibility of using surface topography for guidance of different biological molecules and cells is a relevant topic that can be applied to a wide research activity. This study investigated the...
A double layered state space construction method for reinforcement learning agents (2004)
In this paper, we propose a new double-layered state space construction method, which consists of Fritzke's Growing Neural Gas algorithm and a class management mechanism of GNG units. The...
No abstract available.
Ohbayashi, T., Shimada, M., Nakadai, T., Wada, T., Handa, H., Tamura, T.
The TBP‐like protein (TLP/TRF2/TLF), which belongs to the TBP family of proteins, is present in all metazoan organisms. Although the human TLP has been reported to interfere with transcription...
Evolutionary constitution of game player agents (2002)
In this paper, we propose a constitution method of game player agent that adopts a neural network as a state evaluation function for the game player, and evolves its weights and structure by...
Handa, H., Katai, O., Konishi, T., Baba, M.
We discuss adaptability of evolutionary computations in dynamic environments. We introduce two classes of dynamic environments which are utilizing the notion of constraint satisfaction problems:...
Casein kinase II interacts with the bZIP domains of several transcription factors (1998)
Yamaguchi, Y, Wada, T, Suzuki, F, Takagi, T, Hasegawa, J, Handa, H
The State of Europium Complexes on Alumina Surface (1997)
Yoshida, T., Tanaka, T., Yoshida, S., Handa, H., Hikita, S., Baba, T., ...
Amide complexes of europium prepared by dissolving Eu in a liquid ammonia were fixed on alumina and heated at various temperatures in vacuo. The activity of the samples for the isomerization of...
Short-term effect of fresh disk allograph on the osteoarthrotic sheep temporomandibular joint (1997)
The State of Europium Complexes on Alumina Surface (1997)
Yoshida, T., Tanaka, T., Yoshida, S., Handa, H., Hikita, S., Baba, T., ...
Amide complexes of europium prepared by dissolving Eu in a liquid ammonia were fixed on alumina and heated at various temperatures in vacuo. The activity of the samples for the isomerization of...
The State of Europium Complexes on Alumina Surface (1997)
Yoshida, T., Tanaka, T., Yoshida, S., Handa, H., Hikita, S., Baba, T., ...
Amide complexes of europium prepared by dissolving Eu in a liquid ammonia were fixed on alumina and heated at various temperatures in vacuo. The activity of the samples for the isomerization of...
Short-term effect of fresh disk allograph on the osteoarthrotic sheep temporomandibular joint (1997)
Copurification of casein kinase II with transcription factor ATF/E4TF3 (1996)
Wada, T, Takagi, T, Yamaguchi, Y, Kawase, H, Hiramoto, M, Ferdous, A, ...
Replication factor encoded by a putative oncogene, set, associated with myeloid leukemogenesis.
Nagata, K, Kawase, H, Handa, H, Yano, K, Yamasaki, M, Ishimi, Y, ...
DNA replication of the adenovirus genome complexed with viral core proteins is dependent on the host factor designated template activating factor I (TAF-I) in addition to factors required for...
Mizutani, M, Ohta, T, Watanabe, H, Handa, H, Hirose, S
Transcription of the fibroin gene can be reconstituted with partially purified components from HeLa cells. Transcription factors IIB, IID, and IIE and RNA polymerase II are required for accurate...
Copurification of casein kinase II with transcription factor ATF/E4TF3.
Wada, T, Takagi, T, Yamaguchi, Y, Kawase, H, Hiramoto, M, Ferdous, A, ...
We have developed a simple method to purify sequence-specific DNA-binding proteins directly from crude cell extracts by using DNA affinity latex beads. The method enabled us to purify not only...
Sawa, C, Goto, M, Suzuki, F, Watanabe, H, Sawada, J, Handa, H
Transcription factor E4TF1 is the human homolog of GABP and has been renamed hGABP (human GABP). hGABP is composed of two types of subunits; hGABP beta1/E4TF1-53 and the ets-related protein hGABP...
Casein kinase II interacts with the bZIP domains of several transcription factors.
Yamaguchi, Y, Wada, T, Suzuki, F, Takagi, T, Hasegawa, J, Handa, H
Casein kinase II (CKII) is thought to regulate a broad range of transcription factors, but its mode of action is not well characterized. We previously showed that CKII is co-purified with the ATF...
Ohbayashi, T., Shimada, M., Nakadai, T., Wada, T., Handa, H., Tamura, T.
The TBP-like protein (TLP/TRF2/TLF), which belongs to the TBP family of proteins, is present in all metazoan organisms. Although the human TLP has been reported to interfere with transcription from...
Mechanism for inhibition of influenza virus RNA polymerase activity by matrix protein.
Watanabe, K, Handa, H, Mizumoto, K, Nagata, K
Influenza virus M1 protein has been shown to inhibit the transcription catalyzed by viral ribonucleoprotein complexes isolated from virions. Here, this inhibition mechanism was studied with the...
A nuclear factor (NF2d9) that binds to the male-specific P450 (Cyp 2d-9) gene in mouse liver.
Sueyoshi, T, Kobayashi, R, Nishio, K, Aida, K, Moore, R, Wada, T, ...
Expression of the Cyp 2d-9 (steroid 16 alpha-hydroxylase) gene in mouse liver is male specific in such Mus musculus domesticus strains as FVB/N, whereas the corresponding P450 genes in the wild mouse...
Wada, T, Watanabe, H, Usuda, Y, Handa, H
The transcription factor ATF/E4TF3 stimulates transcription from the adenovirus early region 4 (E4) promoter by binding to specific promoter elements. Among the multiple forms of ATF/E4TF3, two forms...
Mitogenic activity of the adenovirus type 12 E1A gene induced by hormones in rat cells.
Oda, K, Masuda-Murata, M, Shiroki, K, Handa, H
Several lines of rat 3Y1 cells in which expression of the adenovirus type 12 E1A gene can be regulated by dexamethasone were established by introduction of recombinant vector DNA containing the...
Kawaguchi, H, Asai, A, Ohtsuka, Y, Watanabe, H, Wada, T, Handa, H
A simple method with the use of affinity latex particles has been developed for the fast and efficient purification of sequence-specific DNA-binding proteins on the basis of their ability to...
Akahori, Y, Handa, H, Imai, K, Abe, M, Kameyama, K, Hibiya, M, ...
Noncoding regions within the cluster of immunoglobulin heavy chain constant genes in the human genome contained a number of repeats. In the mu-delta intron, two repeating units were contained. One...
Nishigaki, T, Hanaka, S, Kingston, R E, Handa, H
We constructed a series of mutations that delete sequences in the promoter region of the early-region IV (EIV) promoter of adenovirus type 5. We fused these promoter mutations to the coding sequences...
Watanabe, H, Imai, T, Sharp, P A, Handa, H
Two kinds of trans-acting factors that regulate transcription from the promoter of the adenovirus early-region 4 (E4) have been identified by reconstituting nuclear extracts of HeLa cells. They were...
Hanaka, S, Nishigaki, T, Sharp, P A, Handa, H
A series of deletion mutants spanning the promoter of the adenovirus early-region IV (EIV) gene were tested for transcriptional activity, using both in vitro and in vivo assays. Four distinct domains...
A series of deletion mutants spanning the adenovirus early region IV (EIV) promoter were tested for transcription activity in vitro. At least three elements were found to be important for maximal...
Sequential transcription-translation of simian virus 40 by using mammalian cell extracts.
Cepko, C L, Hansen, U, Handa, H, Sharp, P A
Ribonucleic acids (RNAs) transcribed in vitro by using the whole-cell extract system of Manley et al. (Proc. Natl. Acad. Sci. U.S.A. 77:3855-3859, 1980) were tested for their efficiency and fidelity...
Transcriptional activation through the tetrameric complex formation of E4TF1 subunits.
Sawada, J, Goto, M, Sawa, C, Watanabe, H, Handa, H
Transcription factor E4TF1 is composed of two types of subunit, an ets-related DNA binding protein, E4TF1-60, and its associated proteins with four tandemly repeated Notch-ankyrin motifs, E4TF1-53...
Generation of functional clonal cell lines from human bone marrow stroma.
Five clonal human bone marrow stromal cell lines were isolated from the adherent cell populations in long-term liquid cultures after transfection with the recombinant plasmid pSV3gpt. All the...
Transcription in the reverse orientation at either terminus of the adenovirus type 5 genome.
Ooyama, S, Imai, T, Hanaka, S, Handa, H
The right terminus of the adenovirus type 5 (Ad5) genome, which contained the early-region 4 (E4) promoter, was capable of initiating transcription in the reverse orientation in in vitro and in vivo...
Helper factor(s) for growth of adeno-associated virus in cells transformed by adenovirus 12.
Handa, H, Shiroki, K, Shimojo, H
Evidence is presented that a helper factor(s) for growth of adeno-associated virus (AAV) is present in cells transformed by adenovirus type 12 (Ad12). The growth of AAV was observed in heterokaryons...
Affinity purification of transcription factor IIA from HeLa cell nuclear extracts.
Usuda, Y, Kubota, A, Berk, A J, Handa, H
One of the general transcription factors, TFIIA, was purified to homogeneity from HeLa cell nuclear extracts by yeast TFIID affinity chromatography. Human TFIIA had a molecular weight of...
Isolation of the viral DNA replication complex from adeno-associated virus type 1-infected cells.
The replication complex active in adeno-associated virus type 1 (AAV-1) DNA synthesis in vitro was solubilized, with a nonionic detergent, from the nuclei of human embryonic kidney cells coinfected...
Regulation of influenza virus RNA polymerase activity by cellular and viral factors.
Shimizu, K, Handa, H, Nakada, S, Nagata, K
An in vitro RNA synthesis system mimicking replication of genomic influenza virus RNA was developed with nuclear extracts prepared from influenza virus-infected HeLa cells using exogenously added RNA...
Wada, T, Takagi, T, Yamaguchi, Y, Watanabe, D, Handa, H
Recently, a positive and a negative elongation factor, implicated in 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB) inhibition of transcription elongation, has been identified. P-TEFb is a...
Caspase-mediated cleavage of eukaryotic translation initiation factor subunit 2alpha.
Satoh, S, Hijikata, M, Handa, H, Shimotohno, K
Eukaryotic translation initiation factor 2alpha (eIF-2alpha), a target molecule of the interferon-inducible double-stranded-RNA-dependent protein kinase (PKR), was cleaved in apoptotic Saos-2 cells...
Missense mutation in the alternative splice region of the PAX6 gene in eye anomalies.
Azuma, N, Yamaguchi, Y, Handa, H, Hayakawa, M, Kanai, A, Yamada, M
The PAX6 gene is involved in ocular morphogenesis, and PAX6 mutations have been detected in various types of ocular anomalies, including aniridia, Peters anomaly, corneal dystrophy, congenital...
cDNA cloning of transcription factor E4TF1 subunits with Ets and notch motifs.
Watanabe, H, Sawada, J, Yano, K, Yamaguchi, K, Goto, M, Handa, H
E4TF1 was originally identified as one of the transcription factors responsible for adenovirus E4 gene transcription. It is composed of two subunits, a DNA binding protein with a molecular mass of 60...
Transcription factor E4TF1 contains two subunits with different functions.
Watanabe, H, Wada, T, Handa, H
The transcription factor E4TF1 stimulates transcription from the adenovirus early region 4 promoter by binding to a specific promoter element. E4TF1 has been purified to homogeneity from HeLa cells...
Replication factor encoded by a putative oncogene, set, associated with myeloid leukemogenesis.
Nagata, K, Kawase, H, Handa, H, Yano, K, Yamasaki, M, Ishimi, Y, ...
DNA replication of the adenovirus genome complexed with viral core proteins is dependent on the host factor designated template activating factor I (TAF-I) in addition to factors required for...
Mizutani, M, Ohta, T, Watanabe, H, Handa, H, Hirose, S
Transcription of the fibroin gene can be reconstituted with partially purified components from HeLa cells. Transcription factors IIB, IID, and IIE and RNA polymerase II are required for accurate...
Copurification of casein kinase II with transcription factor ATF/E4TF3.
Wada, T, Takagi, T, Yamaguchi, Y, Kawase, H, Hiramoto, M, Ferdous, A, ...
We have developed a simple method to purify sequence-specific DNA-binding proteins directly from crude cell extracts by using DNA affinity latex beads. The method enabled us to purify not only...
Sawa, C, Goto, M, Suzuki, F, Watanabe, H, Sawada, J, Handa, H
Transcription factor E4TF1 is the human homolog of GABP and has been renamed hGABP (human GABP). hGABP is composed of two types of subunits; hGABP beta1/E4TF1-53 and the ets-related protein hGABP...
Casein kinase II interacts with the bZIP domains of several transcription factors.
Yamaguchi, Y, Wada, T, Suzuki, F, Takagi, T, Hasegawa, J, Handa, H
Casein kinase II (CKII) is thought to regulate a broad range of transcription factors, but its mode of action is not well characterized. We previously showed that CKII is co-purified with the ATF...
Ohbayashi, T., Shimada, M., Nakadai, T., Wada, T., Handa, H., Tamura, T.
The TBP-like protein (TLP/TRF2/TLF), which belongs to the TBP family of proteins, is present in all metazoan organisms. Although the human TLP has been reported to interfere with transcription from...
Mechanism for inhibition of influenza virus RNA polymerase activity by matrix protein.
Watanabe, K, Handa, H, Mizumoto, K, Nagata, K
Influenza virus M1 protein has been shown to inhibit the transcription catalyzed by viral ribonucleoprotein complexes isolated from virions. Here, this inhibition mechanism was studied with the...
A nuclear factor (NF2d9) that binds to the male-specific P450 (Cyp 2d-9) gene in mouse liver.
Sueyoshi, T, Kobayashi, R, Nishio, K, Aida, K, Moore, R, Wada, T, ...
Expression of the Cyp 2d-9 (steroid 16 alpha-hydroxylase) gene in mouse liver is male specific in such Mus musculus domesticus strains as FVB/N, whereas the corresponding P450 genes in the wild mouse...
Wada, T, Watanabe, H, Usuda, Y, Handa, H
The transcription factor ATF/E4TF3 stimulates transcription from the adenovirus early region 4 (E4) promoter by binding to specific promoter elements. Among the multiple forms of ATF/E4TF3, two forms...
Mitogenic activity of the adenovirus type 12 E1A gene induced by hormones in rat cells.
Oda, K, Masuda-Murata, M, Shiroki, K, Handa, H
Several lines of rat 3Y1 cells in which expression of the adenovirus type 12 E1A gene can be regulated by dexamethasone were established by introduction of recombinant vector DNA containing the...
Kawaguchi, H, Asai, A, Ohtsuka, Y, Watanabe, H, Wada, T, Handa, H
A simple method with the use of affinity latex particles has been developed for the fast and efficient purification of sequence-specific DNA-binding proteins on the basis of their ability to...
Akahori, Y, Handa, H, Imai, K, Abe, M, Kameyama, K, Hibiya, M, ...
Noncoding regions within the cluster of immunoglobulin heavy chain constant genes in the human genome contained a number of repeats. In the mu-delta intron, two repeating units were contained. One...
cDNA cloning of transcription factor E4TF1 subunits with Ets and notch motifs.
Watanabe, H, Sawada, J, Yano, K, Yamaguchi, K, Goto, M, Handa, H
E4TF1 was originally identified as one of the transcription factors responsible for adenovirus E4 gene transcription. It is composed of two subunits, a DNA binding protein with a molecular mass of 60...
Nishigaki, T, Hanaka, S, Kingston, R E, Handa, H
We constructed a series of mutations that delete sequences in the promoter region of the early-region IV (EIV) promoter of adenovirus type 5. We fused these promoter mutations to the coding sequences...
Watanabe, H, Imai, T, Sharp, P A, Handa, H
Two kinds of trans-acting factors that regulate transcription from the promoter of the adenovirus early-region 4 (E4) have been identified by reconstituting nuclear extracts of HeLa cells. They were...
Hanaka, S, Nishigaki, T, Sharp, P A, Handa, H
A series of deletion mutants spanning the promoter of the adenovirus early-region IV (EIV) gene were tested for transcriptional activity, using both in vitro and in vivo assays. Four distinct domains...
A series of deletion mutants spanning the adenovirus early region IV (EIV) promoter were tested for transcription activity in vitro. At least three elements were found to be important for maximal...
Sequential transcription-translation of simian virus 40 by using mammalian cell extracts.
Cepko, C L, Hansen, U, Handa, H, Sharp, P A
Ribonucleic acids (RNAs) transcribed in vitro by using the whole-cell extract system of Manley et al. (Proc. Natl. Acad. Sci. U.S.A. 77:3855-3859, 1980) were tested for their efficiency and fidelity...
Transcriptional activation through the tetrameric complex formation of E4TF1 subunits.
Sawada, J, Goto, M, Sawa, C, Watanabe, H, Handa, H
Transcription factor E4TF1 is composed of two types of subunit, an ets-related DNA binding protein, E4TF1-60, and its associated proteins with four tandemly repeated Notch-ankyrin motifs, E4TF1-53...
Generation of functional clonal cell lines from human bone marrow stroma.
Five clonal human bone marrow stromal cell lines were isolated from the adherent cell populations in long-term liquid cultures after transfection with the recombinant plasmid pSV3gpt. All the...
Transcription in the reverse orientation at either terminus of the adenovirus type 5 genome.
Ooyama, S, Imai, T, Hanaka, S, Handa, H
The right terminus of the adenovirus type 5 (Ad5) genome, which contained the early-region 4 (E4) promoter, was capable of initiating transcription in the reverse orientation in in vitro and in vivo...
Helper factor(s) for growth of adeno-associated virus in cells transformed by adenovirus 12.
Handa, H, Shiroki, K, Shimojo, H
Evidence is presented that a helper factor(s) for growth of adeno-associated virus (AAV) is present in cells transformed by adenovirus type 12 (Ad12). The growth of AAV was observed in heterokaryons...
Affinity purification of transcription factor IIA from HeLa cell nuclear extracts.
Usuda, Y, Kubota, A, Berk, A J, Handa, H
One of the general transcription factors, TFIIA, was purified to homogeneity from HeLa cell nuclear extracts by yeast TFIID affinity chromatography. Human TFIIA had a molecular weight of...
Isolation of the viral DNA replication complex from adeno-associated virus type 1-infected cells.
The replication complex active in adeno-associated virus type 1 (AAV-1) DNA synthesis in vitro was solubilized, with a nonionic detergent, from the nuclei of human embryonic kidney cells coinfected...
Regulation of influenza virus RNA polymerase activity by cellular and viral factors.
Shimizu, K, Handa, H, Nakada, S, Nagata, K
An in vitro RNA synthesis system mimicking replication of genomic influenza virus RNA was developed with nuclear extracts prepared from influenza virus-infected HeLa cells using exogenously added RNA...
Transcription factor E4TF1 contains two subunits with different functions.
Watanabe, H, Wada, T, Handa, H
The transcription factor E4TF1 stimulates transcription from the adenovirus early region 4 promoter by binding to a specific promoter element. E4TF1 has been purified to homogeneity from HeLa cells...
Wada, T, Takagi, T, Yamaguchi, Y, Watanabe, D, Handa, H
Recently, a positive and a negative elongation factor, implicated in 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB) inhibition of transcription elongation, has been identified. P-TEFb is a...
Caspase-mediated cleavage of eukaryotic translation initiation factor subunit 2alpha.
Satoh, S, Hijikata, M, Handa, H, Shimotohno, K
Eukaryotic translation initiation factor 2alpha (eIF-2alpha), a target molecule of the interferon-inducible double-stranded-RNA-dependent protein kinase (PKR), was cleaved in apoptotic Saos-2 cells...
Missense mutation in the alternative splice region of the PAX6 gene in eye anomalies.
Azuma, N, Yamaguchi, Y, Handa, H, Hayakawa, M, Kanai, A, Yamada, M
The PAX6 gene is involved in ocular morphogenesis, and PAX6 mutations have been detected in various types of ocular anomalies, including aniridia, Peters anomaly, corneal dystrophy, congenital...
Keyaki, A, Kuribayashi, K, Sakaguchi, S, Masuda, T, Yamashita, J, Handa, H, ...
We demonstrated the efficacy of a long-term cultured cytotoxic T-lymphocyte line, CTLL-D4, on tumour growth inhibition using athymic nude mice as recipients. CTLL-D4, specific for a unique surface...
PAX6 expression in the developing human eye
Nishina, S., Kohsaka, S., Yamaguchi, Y., Handa, H., Kawakami, A., Fujisawa, H., ...
AIMS—To investigate the changes in PAX6 expression in the developing human eye. METHODS—Six developing human eyes from 6 to 22 weeks' gestation were evaluated. Frozen sections were...
Abducens nerve palsy as initial symptom of trigeminal schwannoma.
Yamashita, J, Asato, R, Handa, H, Nakao, S, Ogata, M
The insidious onset and slow progression of symptoms may cause delay in the recognition of trigeminal schwannomas. Two cases with abducens nerve palsy as an initial symptom are reported, in both of...
Thalamic tumours invading the brain stem produce crossed cerebellar diaschisis demonstrated by PET.
Fukuyama, H, Kameyama, M, Harada, K, Fujimoto, N, Kobayashi, A, Taki, W, ...
Two cases of thalamic tumour, spreading through the midbrain into the pons ventrolaterally, showed suppression of cerebellar blood flow on the opposite side to the tumour, and also showed reduction...
Immortalisation of human oesophageal epithelial cells by a recombinant SV40 adenovirus vector.
Inokuchi, S., Handa, H., Imai, T., Makuuchi, H., Kidokoro, M., Tohya, H., ...
We introduced the origin-defective SV40 early gene into cultured human oesophageal epithelial cells by infection of a recombinant SV40 adenovirus vector. The virus-infected cells formed colonies 3-4...