Probing the structure of the Escherichia coli 10Sa RNA (tmRNA) (1997)
Felden, B., Himeno, H., Muto, A., McCutcheon, J. P., Atkins, J. F., Gesteland, R. F.
Prokaryotes have three amino acid-specific class II tRNAs that possess a characteristic long variable arm, tRNASer, tRNALeuand tRNATyr, while eukaryotes have only two, tRNASerand tRNALeu. Because of...
Nameki, N, Chattopadhyay, P, Himeno, H, Muto, A, Kawai, G
Transfer-messenger RNA (tmRNA) is a unique molecule that combines properties from both tRNA and mRNA, and facilitates a novel translation reaction termed trans -translation. According to phylogenetic...
Identity elements of Saccharomyces cerevisiae tRNA(His).
Nameki, N, Asahara, H, Shimizu, M, Okada, N, Himeno, H
Recognition of tRNA(His) by Saccharomyces cerevisiae histidyl-tRNA synthetase was studied using in vitro transcripts. Histidine tRNA is unique in possessing an extra nucleotide, G-1, at the 5' end....
Identity determinants of E. coli tryptophan tRNA.
Himeno, H, Hasegawa, T, Asahara, H, Tamura, K, Shimizu, M
The first base pair of the acceptor stem A1-U72 and the discriminator base G73, as well as the anticodon nucleotides, characterize the tryptophan tRNA in E. coli. To determine the contribution of...
Conversion of aminoacylation specificity from tRNA(Tyr) to tRNA(Ser) in vitro.
Himeno, H, Hasegawa, T, Ueda, T, Watanabe, K, Shimizu, M
The discrimination mechanism between tRNA(Ser) and tRNA(Tyr) was studied using various in vitro transcripts of E. coli tRNATyr variants. The insertion of only two nucleotides into the variable stem...
Role of the extra G-C pair at the end of the acceptor stem of tRNA(His) in aminoacylation.
Himeno, H, Hasegawa, T, Ueda, T, Watanabe, K, Miura, K, Shimizu, M
All sequenced histidine tRNAs have one additional nucleotide at the 5' end compared with other tRNA species. To investigate the role of this unique structure in aminoacylation, we constructed in...
tRNA-like structures in 10Sa RNAs of Mycoplasma capricolum and Bacillus subtilis.
Ushida, C, Himeno, H, Watanabe, T, Muto, A
The stable RNAs, whose sequences are homologous to 10Sa RNA of Escherichia coli, have been isolated from Mycoplasma capricolum and Bacillus subtilis, both belonging to the Gram-positive bacterial...
Felden, B, Hanawa, K, Atkins, J F, Himeno, H, Muto, A, Gesteland, R F, ...
Escherichia coli tmRNA functions uniquely as both tRNA and mRNA and possesses structural elements similar to canonical tRNAs. To test whether this mimicry extends to post-transcriptional...
Probing the structure of the Escherichia coli 10Sa RNA (tmRNA).
Felden, B, Himeno, H, Muto, A, McCutcheon, J P, Atkins, J F, Gesteland, R F
The conformation of the Escherichia coli 10Sa RNA (tmRNA) in solution was investigated using chemical and enzymatic probes. Single- and double-stranded domains were identified by hydrolysis of tmRNA...
Lee, S, Ishii, M, Tadaki, T, Muto, A, Himeno, H
tmRNA facilitates a novel translation, trans-translation, in which a ribosome can switch between translation of a truncated mRNA and the tmRNA's tag sequence. The mechanism underlying resumption of...
In vitro study of E.coli tRNA(Arg) and tRNA(Lys) identity elements.
Tamura, K, Himeno, H, Asahara, H, Hasegawa, T, Shimizu, M
Various tRNA transcripts were constructed to study the identity elements of E.coli tRNA(Arg) and tRNA(Lys). Exchange of the anticodon of the major tRNA(Arg) from ACG to either CCG or CCU did not...
Nucleotide Sequence and Gene Organization of the Starfish Asterina Pectinifera Mitochondrial Genome
Asakawa, S., Himeno, H., Miura, K. I., Watanabe, K.
The 16,260-bp mitochondrial DNA (mtDNA) from the starfish Asterina pectinifera has been sequenced. The genes for 13 proteins, two rRNAs and 22 tRNAs are organized in an extremely economical fashion,...
Prokaryotes have three amino acid-specific class II tRNAs that possess a characteristic long variable arm, tRNASer, tRNALeuand tRNATyr, while eukaryotes have only two, tRNASerand tRNALeu. Because of...
Nameki, N, Chattopadhyay, P, Himeno, H, Muto, A, Kawai, G
Transfer-messenger RNA (tmRNA) is a unique molecule that combines properties from both tRNA and mRNA, and facilitates a novel translation reaction termed trans -translation. According to phylogenetic...
Identity elements of Saccharomyces cerevisiae tRNA(His).
Nameki, N, Asahara, H, Shimizu, M, Okada, N, Himeno, H
Recognition of tRNA(His) by Saccharomyces cerevisiae histidyl-tRNA synthetase was studied using in vitro transcripts. Histidine tRNA is unique in possessing an extra nucleotide, G-1, at the 5' end....
In vitro study of E.coli tRNA(Arg) and tRNA(Lys) identity elements.
Tamura, K, Himeno, H, Asahara, H, Hasegawa, T, Shimizu, M
Various tRNA transcripts were constructed to study the identity elements of E.coli tRNA(Arg) and tRNA(Lys). Exchange of the anticodon of the major tRNA(Arg) from ACG to either CCG or CCU did not...
Identity determinants of E. coli tryptophan tRNA.
Himeno, H, Hasegawa, T, Asahara, H, Tamura, K, Shimizu, M
The first base pair of the acceptor stem A1-U72 and the discriminator base G73, as well as the anticodon nucleotides, characterize the tryptophan tRNA in E. coli. To determine the contribution of...
Conversion of aminoacylation specificity from tRNA(Tyr) to tRNA(Ser) in vitro.
Himeno, H, Hasegawa, T, Ueda, T, Watanabe, K, Shimizu, M
The discrimination mechanism between tRNA(Ser) and tRNA(Tyr) was studied using various in vitro transcripts of E. coli tRNATyr variants. The insertion of only two nucleotides into the variable stem...
Role of the extra G-C pair at the end of the acceptor stem of tRNA(His) in aminoacylation.
Himeno, H, Hasegawa, T, Ueda, T, Watanabe, K, Miura, K, Shimizu, M
All sequenced histidine tRNAs have one additional nucleotide at the 5' end compared with other tRNA species. To investigate the role of this unique structure in aminoacylation, we constructed in...
tRNA-like structures in 10Sa RNAs of Mycoplasma capricolum and Bacillus subtilis.
Ushida, C, Himeno, H, Watanabe, T, Muto, A
The stable RNAs, whose sequences are homologous to 10Sa RNA of Escherichia coli, have been isolated from Mycoplasma capricolum and Bacillus subtilis, both belonging to the Gram-positive bacterial...
Felden, B, Hanawa, K, Atkins, J F, Himeno, H, Muto, A, Gesteland, R F, ...
Escherichia coli tmRNA functions uniquely as both tRNA and mRNA and possesses structural elements similar to canonical tRNAs. To test whether this mimicry extends to post-transcriptional...
Nucleotide Sequence and Gene Organization of the Starfish Asterina Pectinifera Mitochondrial Genome
Asakawa, S., Himeno, H., Miura, K. I., Watanabe, K.
The 16,260-bp mitochondrial DNA (mtDNA) from the starfish Asterina pectinifera has been sequenced. The genes for 13 proteins, two rRNAs and 22 tRNAs are organized in an extremely economical fashion,...
Probing the structure of the Escherichia coli 10Sa RNA (tmRNA).
Felden, B, Himeno, H, Muto, A, McCutcheon, J P, Atkins, J F, Gesteland, R F
The conformation of the Escherichia coli 10Sa RNA (tmRNA) in solution was investigated using chemical and enzymatic probes. Single- and double-stranded domains were identified by hydrolysis of tmRNA...