Olawole, O., Jacobsen, E., Timmers, J.F.P., Gilbert, H.J., Blake, W., Knox, J.P., ...
We have compared heterologous expression of two types of carbohydrate binding module (CBM) in tobacco cell walls. These are the promiscuous CBM29 modules (a tandem CBM29-1-2 and its single derivative...
Taylor, E.J., Goyal, A., Prates, J.A.M., Money, V.A., Ferry, N., ...
One of the most intriguing features of the 90 glycoside hydrolase families (GHs) is the range of specificities displayed by different members of the same family, whereas the catalytic apparatus and...
Proctor, M.R., Taylor, E.J., Nurizzo, D., Turkenburg, J.P., Lloyd, R.M., Vardakou, M., ...
Enzymes acting on polymeric substrates are frequently classified as exo or endo, reflecting their preference for, or ignorance of, polymer chain ends. Most biotechnological applications, especially...
Ji, Q., Vincken, J.P., Bolam, D.N., Gilbert, H.J., ...
Granule size is an important parameter when using starch in industrial applications. An artificial tandem repeat of a family 20 starch-binding domain (SBD2) was engineered by two copies of the SBD...
Vincent, F., Charnock, S.J., Verschueren, K.H.G., Turkenburg, J.P., Scott, D.J., Offen, W.A., ...
Esterases and deacetylases active on carbohydrate ligands have been classified into 14 families based upon amino acid sequence similarities. Enzymes from carbohydrate esterase family seven (CE-7) are...
A new family of rhamnogalacturonan lyases contains an enzyme that binds to cellulose (2001)
McKie, V.A., Vincken, J.P., Voragen, A.G.J., Stimson, E., Gilbert, H.J.
Pseudomonas cellulosa is an aerobic bacterium that synthesizes an extensive array of modular cellulases and hemicellulases, which have a modular architecture consisting of catalytic domains and...
Role of hydrogen bonding in the interaction between a xylan binding module and xylan (2001)
Xie, H.F., Bolam, D.N., Nagy, T., Szabo, L., Cooper, A., Simpson, P.J., ...
NMR studies of the internal family 2b carbohydrate binding module (CBM2b-1) of Cellulomonas fimi xylanase 11A have identified six polar residues and two aromatic residues that interact with its...
Role of hydrogen bonding in the interaction between a xylan binding module and xylan
Xie, H.F., Bolam, D.N., Nagy, T., Szabo, L., Cooper, A., Simpson, P.J., ...
NMR studies of the internal family 2b carbohydrate binding module (CBM2b-1) of Cellulomonas fimi xylanase 11A have identified six polar residues and two aromatic residues that interact with its...
Probiotics Shown To Change Bacterial Community Structure in the Avian Gastrointestinal Tract
Netherwood, Trudy, Gilbert, H. J., Parker, D. S., O’Donnell, A. G.
Culturing and molecular techniques were used to monitor changes in the bacterial flora of the avian gastrointestinal (GI) tract following introduction of genetically modified (GM) and unmodified...
Gene Transfer in the Gastrointestinal Tract
Netherwood, Trudy, Bowden, R., Harrison, P., O’Donnell, A. G., Parker, D. S., Gilbert, H. J.
The maximum in vivo transfer rate of plasmid pAMβ1 in the gut was 0.03 transconjugant per recipient cell, and this rate could be simulated in vitro only by forced filter mating. Transfer was not...
Bartolomé, B, Faulds, C B, Kroon, P A, Waldron, K, Gilbert, H J, Hazlewood, G, ...
Diferulate esters strengthen and cross-link primary plant cell walls and help to defend the plant from invading microbes. Phenolics also limit the degradation of plant cell walls by saprophytic...
Expression of a Clostridium thermocellum endoglucanase gene in Lactobacillus plantarum.
Bates, E E, Gilbert, H J, Hazlewood, G P, Huckle, J, Laurie, J I, Mann, S P
Recombinant plasmid pM25 containing the celE gene of Clostridium thermocellum, which codes for an enzymatically active endoglucanase, was transformed into Lactobacillus plantarum by electroporation....
Gilbert, H J, Clarke, I N, Gibson, R K, Stephenson, J R, Tully, M
A genomic library of Rhodosporidium toruloides DNA was constructed in bacteriophage lambda 1059. Recombinant phage containing phenylalanine ammonia lyase (PAL) gene sequences were identified by using...
Synthesis and degradation of phenylalanine ammonia-lyase of Rhodosporidium toruloides.
The regulation of the enzyme phenylalanine ammonia-lyase (PAL), which is of potential use in oral treatment of phenylketonuria, was investigated. Antiserum against PAL was prepared and was shown to...
Ferreira, L M, Durrant, A J, Hall, J, Hazlewood, G P, Gilbert, H J
Xylanase A (XYLA) from Pseudomonas fluorescens subspecies cellulosa shows sequence conservation with two endoglucanases from the same organism. The conserved sequence in XYLA, consisting of the...
Rixon, J E, Ferreira, L M, Durrant, A J, Laurie, J I, Hazlewood, G P, Gilbert, H J
A genomic library of Pseudomonas fluorescens subsp. cellulosa DNA constructed in pUC18 and expressed in Escherichia coli was screened for recombinants expressing 4-methylumbelliferyl beta-D-glucoside...
Ferreira, L M, Wood, T M, Williamson, G, Faulds, C, Hazlewood, G P, Black, G W, ...
The 5' regions of genes xynB and xynC, coding for a xylanase and arabinofuranosidase respectively, are identical and are reiterated four times within the Pseudomonas fluorescens subsp. cellulosa...
Hall, J, Black, G W, Ferreira, L M, Millward-Sadler, S J, Ali, B R, Hazlewood, G P, ...
A genomic library of Pseudomonas fluorescens subsp. cellulosa DNA, constructed in lambda ZAPII, was screened for carboxymethyl-cellulase activity. The pseudomonad insert from a recombinant phage...
Millward-Sadler, S J, Davidson, K, Hazlewood, G P, Black, G W, Gilbert, H J, Clarke, J H
To test the hypothesis that selective pressure has led to the retention of cellulose-binding domains (CBDs) by hemicellulase enzymes from aerobic bacteria, four new xylanase (xyn) genes from two...
A non-modular endo-beta-1,4-mannanase from Pseudomonas fluorescens subspecies cellulosa.
Braithwaite, K L, Black, G W, Hazlewood, G P, Ali, B R, Gilbert, H J
Pseudomonas fluorescens subsp. cellulosa when cultured in the presence of carob galactomannan degraded the polysaccharide. To isolate gene(s) from P. fluorescens subsp. cellulosa encoding...
Fontes, C M, Hazlewood, G P, Morag, E, Hall, J, Hirst, B H, Gilbert, H J
A genomic library of Clostridium thermocellum DNA constructed in lambda ZAPII was screened for xylanase-expressing clones. Cross-hybridization experiments revealed a new xylanase gene isolated from...
A modular xylanase containing a novel non-catalytic xylan-specific binding domain.
Black, G W, Hazlewood, G P, Millward-Sadler, S J, Laurie, J I, Gilbert, H J
Xylanase D (XYLD) from Cellulomonas fimi contains a C-terminal cellulose-binding domain (CBD) and an internal domain that exhibits 65% sequence identity with the C-terminal CBD. Full-length XYLD...
Zhou, L, Xue, G P, Orpin, C G, Black, G W, Gilbert, H J, Hazlewood, G P
The cDNA designated celB from the anaerobic rumen fungus Neocallimastix patriciarum contained a single open reading frame of 1422 bp coding for a protein (CelB) of M(r) 53,070. CelB expressed by...
Black, G W, Hazlewood, G P, Xue, G P, Orpin, C G, Gilbert, H J
A Neocallimastix patriciarum cDNA library was screened for xylanase-expressing clones, which were distinct from the previously characterized N. patriciarum xynA cDNA encoding xylanase A. A single...
Kellett, L E, Poole, D M, Ferreira, L M, Durrant, A J, Hazlewood, G P, Gilbert, H J
The complete nucleotide sequence of the Pseudomonas fluorescens subsp. cellulosa xynB gene, encoding an endo-beta-1,4-xylanase (xylanase B; XYLB) has been determined. The structural gene consists of...
Durrant, A J, Hall, J, Hazlewood, G P, Gilbert, H J
Mature endoglucanase E (EGE) from Clostridium thermocellum consists of 780 amino acid residues and has an Mr of 84,016. The N-terminal 334 amino acids comprise a functional catalytic domain....
Gilbert, H J, Lowe, C R, Drabble, W T
Escherichia coli IMP dehydrogenase (EC 1.2.1.14) was purified by affinity chromatography on immobilized nucleotides. The enzyme binds to agarose-bound 8-(6-aminohexyl)-AMP, N6-(6-aminohexyl)-AMP and...
The effect of proteinases on phenylalanine ammonia-lyase from the yeast Rhodotorula glutinis.
Phenylalanine ammonia-lyase (EC 4.3.1.5) of the yeast Rhodotorula glutinis was rapidly inactivated by duodenal juice. It was susceptible to chymotrypsin and subtilisin and to a lesser extent trypsin....
A protein targeting signal that functions in polarized epithelial cells in vivo.
Ali, S, Hall, J, Hazlewood, G P, Hirst, B H, Gilbert, H J
Eukaryotic membrane-associated polypeptides often contain a glycosylphosphatidylinositol (GPI) anchor that signals the attachment of GPI lipids to these proteins. The GPI anchor can function as a...
Black, G W, Rixon, J E, Clarke, J H, Hazlewood, G P, Theodorou, M K, Morris, P, ...
Xylanase A (XYLA) and arabinofuranosidase C (XYLC) from Pseudomonas fluorescens subsp. cellulosa are modular enzymes consisting of discrete cellulose-binding domains (CBDs) and catalytic domains...
McKie, V A, Black, G W, Millward-Sadler, S J, Hazlewood, G P, Laurie, J I, Gilbert, H J
Pseudomonas fluorescens subsp. cellulosa expressed arabinanase activity when grown on media supplemented with arabinan or arabinose. Arabinanase activity was not induced by the inclusion of other...
Bolam, D N, Ciruela, A, McQueen-Mason, S, Simpson, P, Williamson, M P, Rixon, J E, ...
To investigate the mode of action of cellulose-binding domains (CBDs), the Type II CBD from Pseudomonas fluorescens subsp. cellulosa xylanase A (XYLACBD) and cellulase E (CELECBD) were expressed as...
Fernandes, A C, Fontes, C M, Gilbert, H J, Hazlewood, G P, Fernandes, T H, Ferreira, L M
Clostridium thermocellum produces a consortium of plant-cell-wall hydrolases that form a cell-bound multi-enzyme complex called the cellulosome. In the present study two similar xylanase genes, xynU...
Gill, J, Rixon, J E, Bolam, D N, McQueen-Mason, S, Simpson, P J, Williamson, M P, ...
Xylanase A (Pf Xyn10A), in common with several other Pseudomonas fluorescens subsp. cellulosa polysaccharidases, consists of a Type II cellulose-binding domain (CBD), a catalytic domain (Pf...
Fillingham, I J, Kroon, P A, Williamson, G, Gilbert, H J, Hazlewood, G P
A collection of clones, isolated from a Piromyces equi cDNA expression library by immunoscreening with antibodies raised against affinity purified multienzyme fungal cellulase-hemicellulase complex,...
Abou Hachem, M, Nordberg Karlsson, E, Bartonek-Roxâ, E, Raghothama, S, Simpson, P J, Gilbert, H J, ...
The two N-terminally repeated carbohydrate-binding modules (CBM4-1 and CBM4-2) encoded by xyn10A from Rhodothermus marinus were produced in Escherichia coli and purified by affinity chromatography....
A new family of rhamnogalacturonan lyases contains an enzyme that binds to cellulose.
McKie, V A, Vincken, J P, Voragen, A G, Van Den Broek, L A, Stimson, E, Gilbert, H J
Pseudomonas cellulosa is an aerobic bacterium that synthesizes an extensive array of modular cellulases and hemicellulases, which have a modular architecture consisting of catalytic domains and...
Beylot, M H, Emami, K, McKie, V A, Gilbert, H J, Pell, G
In the accompanying paper [Beylot, McKie, Voragen, Doeswijk-Voragen and Gilbert (2001) Biochem. J. 358, 607-614] the chromosome of Pseudomonas cellulosa was shown to contain two genes, abf51A and...
Beylot, M H, McKie, V A, Voragen, A G, Doeswijk-Voragen, C H, Gilbert, H J
To investigate the mechanism by which Pseudomonas cellulosa releases arabinose from polysaccharides and oligosaccharides, a gene library of P. cellulosa genomic DNA was screened for...
Poole, D M, Durrant, A J, Hazlewood, G P, Gilbert, H J
The N-terminal 160 or 267 residues of xylanase A from Pseudomonas fluorescens subsp. cellulosa, containing a non-catalytic cellulose-binding domain (CBD), were fused to the N-terminus of the...
Ferreira, L M, Hazlewood, G P, Barker, P J, Gilbert, H J
A genomic library of Pseudomonas fluorescens subsp. cellulosa DNA was constructed in pUC18 and Escherichia coli recombinants expressing 4-methylumbelliferyl beta-D-cellobioside-hydrolysing activity...
Probiotics Shown To Change Bacterial Community Structure in the Avian Gastrointestinal Tract
Netherwood, Trudy, Gilbert, H. J., Parker, D. S., O’Donnell, A. G.
Culturing and molecular techniques were used to monitor changes in the bacterial flora of the avian gastrointestinal (GI) tract following introduction of genetically modified (GM) and unmodified...
Gene Transfer in the Gastrointestinal Tract
Netherwood, Trudy, Bowden, R., Harrison, P., O’Donnell, A. G., Parker, D. S., Gilbert, H. J.
The maximum in vivo transfer rate of plasmid pAMβ1 in the gut was 0.03 transconjugant per recipient cell, and this rate could be simulated in vitro only by forced filter mating. Transfer was not...
Bartolomé, B, Faulds, C B, Kroon, P A, Waldron, K, Gilbert, H J, Hazlewood, G, ...
Diferulate esters strengthen and cross-link primary plant cell walls and help to defend the plant from invading microbes. Phenolics also limit the degradation of plant cell walls by saprophytic...
Expression of a Clostridium thermocellum endoglucanase gene in Lactobacillus plantarum.
Bates, E E, Gilbert, H J, Hazlewood, G P, Huckle, J, Laurie, J I, Mann, S P
Recombinant plasmid pM25 containing the celE gene of Clostridium thermocellum, which codes for an enzymatically active endoglucanase, was transformed into Lactobacillus plantarum by electroporation....
Gilbert, H J, Clarke, I N, Gibson, R K, Stephenson, J R, Tully, M
A genomic library of Rhodosporidium toruloides DNA was constructed in bacteriophage lambda 1059. Recombinant phage containing phenylalanine ammonia lyase (PAL) gene sequences were identified by using...
Synthesis and degradation of phenylalanine ammonia-lyase of Rhodosporidium toruloides.
The regulation of the enzyme phenylalanine ammonia-lyase (PAL), which is of potential use in oral treatment of phenylketonuria, was investigated. Antiserum against PAL was prepared and was shown to...
Ferreira, L M, Durrant, A J, Hall, J, Hazlewood, G P, Gilbert, H J
Xylanase A (XYLA) from Pseudomonas fluorescens subspecies cellulosa shows sequence conservation with two endoglucanases from the same organism. The conserved sequence in XYLA, consisting of the...
Rixon, J E, Ferreira, L M, Durrant, A J, Laurie, J I, Hazlewood, G P, Gilbert, H J
A genomic library of Pseudomonas fluorescens subsp. cellulosa DNA constructed in pUC18 and expressed in Escherichia coli was screened for recombinants expressing 4-methylumbelliferyl beta-D-glucoside...
Ferreira, L M, Wood, T M, Williamson, G, Faulds, C, Hazlewood, G P, Black, G W, ...
The 5' regions of genes xynB and xynC, coding for a xylanase and arabinofuranosidase respectively, are identical and are reiterated four times within the Pseudomonas fluorescens subsp. cellulosa...
Hall, J, Black, G W, Ferreira, L M, Millward-Sadler, S J, Ali, B R, Hazlewood, G P, ...
A genomic library of Pseudomonas fluorescens subsp. cellulosa DNA, constructed in lambda ZAPII, was screened for carboxymethyl-cellulase activity. The pseudomonad insert from a recombinant phage...
Millward-Sadler, S J, Davidson, K, Hazlewood, G P, Black, G W, Gilbert, H J, Clarke, J H
To test the hypothesis that selective pressure has led to the retention of cellulose-binding domains (CBDs) by hemicellulase enzymes from aerobic bacteria, four new xylanase (xyn) genes from two...
A non-modular endo-beta-1,4-mannanase from Pseudomonas fluorescens subspecies cellulosa.
Braithwaite, K L, Black, G W, Hazlewood, G P, Ali, B R, Gilbert, H J
Pseudomonas fluorescens subsp. cellulosa when cultured in the presence of carob galactomannan degraded the polysaccharide. To isolate gene(s) from P. fluorescens subsp. cellulosa encoding...
Fontes, C M, Hazlewood, G P, Morag, E, Hall, J, Hirst, B H, Gilbert, H J
A genomic library of Clostridium thermocellum DNA constructed in lambda ZAPII was screened for xylanase-expressing clones. Cross-hybridization experiments revealed a new xylanase gene isolated from...
A modular xylanase containing a novel non-catalytic xylan-specific binding domain.
Black, G W, Hazlewood, G P, Millward-Sadler, S J, Laurie, J I, Gilbert, H J
Xylanase D (XYLD) from Cellulomonas fimi contains a C-terminal cellulose-binding domain (CBD) and an internal domain that exhibits 65% sequence identity with the C-terminal CBD. Full-length XYLD...
Zhou, L, Xue, G P, Orpin, C G, Black, G W, Gilbert, H J, Hazlewood, G P
The cDNA designated celB from the anaerobic rumen fungus Neocallimastix patriciarum contained a single open reading frame of 1422 bp coding for a protein (CelB) of M(r) 53,070. CelB expressed by...
Black, G W, Hazlewood, G P, Xue, G P, Orpin, C G, Gilbert, H J
A Neocallimastix patriciarum cDNA library was screened for xylanase-expressing clones, which were distinct from the previously characterized N. patriciarum xynA cDNA encoding xylanase A. A single...
Kellett, L E, Poole, D M, Ferreira, L M, Durrant, A J, Hazlewood, G P, Gilbert, H J
The complete nucleotide sequence of the Pseudomonas fluorescens subsp. cellulosa xynB gene, encoding an endo-beta-1,4-xylanase (xylanase B; XYLB) has been determined. The structural gene consists of...
Durrant, A J, Hall, J, Hazlewood, G P, Gilbert, H J
Mature endoglucanase E (EGE) from Clostridium thermocellum consists of 780 amino acid residues and has an Mr of 84,016. The N-terminal 334 amino acids comprise a functional catalytic domain....
Poole, D M, Durrant, A J, Hazlewood, G P, Gilbert, H J
The N-terminal 160 or 267 residues of xylanase A from Pseudomonas fluorescens subsp. cellulosa, containing a non-catalytic cellulose-binding domain (CBD), were fused to the N-terminus of the...
Ferreira, L M, Hazlewood, G P, Barker, P J, Gilbert, H J
A genomic library of Pseudomonas fluorescens subsp. cellulosa DNA was constructed in pUC18 and Escherichia coli recombinants expressing 4-methylumbelliferyl beta-D-cellobioside-hydrolysing activity...
Gilbert, H J, Lowe, C R, Drabble, W T
Escherichia coli IMP dehydrogenase (EC 1.2.1.14) was purified by affinity chromatography on immobilized nucleotides. The enzyme binds to agarose-bound 8-(6-aminohexyl)-AMP, N6-(6-aminohexyl)-AMP and...
The effect of proteinases on phenylalanine ammonia-lyase from the yeast Rhodotorula glutinis.
Phenylalanine ammonia-lyase (EC 4.3.1.5) of the yeast Rhodotorula glutinis was rapidly inactivated by duodenal juice. It was susceptible to chymotrypsin and subtilisin and to a lesser extent trypsin....
A protein targeting signal that functions in polarized epithelial cells in vivo.
Ali, S, Hall, J, Hazlewood, G P, Hirst, B H, Gilbert, H J
Eukaryotic membrane-associated polypeptides often contain a glycosylphosphatidylinositol (GPI) anchor that signals the attachment of GPI lipids to these proteins. The GPI anchor can function as a...
Black, G W, Rixon, J E, Clarke, J H, Hazlewood, G P, Theodorou, M K, Morris, P, ...
Xylanase A (XYLA) and arabinofuranosidase C (XYLC) from Pseudomonas fluorescens subsp. cellulosa are modular enzymes consisting of discrete cellulose-binding domains (CBDs) and catalytic domains...
McKie, V A, Black, G W, Millward-Sadler, S J, Hazlewood, G P, Laurie, J I, Gilbert, H J
Pseudomonas fluorescens subsp. cellulosa expressed arabinanase activity when grown on media supplemented with arabinan or arabinose. Arabinanase activity was not induced by the inclusion of other...
Bolam, D N, Ciruela, A, McQueen-Mason, S, Simpson, P, Williamson, M P, Rixon, J E, ...
To investigate the mode of action of cellulose-binding domains (CBDs), the Type II CBD from Pseudomonas fluorescens subsp. cellulosa xylanase A (XYLACBD) and cellulase E (CELECBD) were expressed as...
Fernandes, A C, Fontes, C M, Gilbert, H J, Hazlewood, G P, Fernandes, T H, Ferreira, L M
Clostridium thermocellum produces a consortium of plant-cell-wall hydrolases that form a cell-bound multi-enzyme complex called the cellulosome. In the present study two similar xylanase genes, xynU...
Gill, J, Rixon, J E, Bolam, D N, McQueen-Mason, S, Simpson, P J, Williamson, M P, ...
Xylanase A (Pf Xyn10A), in common with several other Pseudomonas fluorescens subsp. cellulosa polysaccharidases, consists of a Type II cellulose-binding domain (CBD), a catalytic domain (Pf...
Fillingham, I J, Kroon, P A, Williamson, G, Gilbert, H J, Hazlewood, G P
A collection of clones, isolated from a Piromyces equi cDNA expression library by immunoscreening with antibodies raised against affinity purified multienzyme fungal cellulase-hemicellulase complex,...
Abou Hachem, M, Nordberg Karlsson, E, Bartonek-Roxâ, E, Raghothama, S, Simpson, P J, Gilbert, H J, ...
The two N-terminally repeated carbohydrate-binding modules (CBM4-1 and CBM4-2) encoded by xyn10A from Rhodothermus marinus were produced in Escherichia coli and purified by affinity chromatography....
A new family of rhamnogalacturonan lyases contains an enzyme that binds to cellulose.
McKie, V A, Vincken, J P, Voragen, A G, Van Den Broek, L A, Stimson, E, Gilbert, H J
Pseudomonas cellulosa is an aerobic bacterium that synthesizes an extensive array of modular cellulases and hemicellulases, which have a modular architecture consisting of catalytic domains and...
Beylot, M H, Emami, K, McKie, V A, Gilbert, H J, Pell, G
In the accompanying paper [Beylot, McKie, Voragen, Doeswijk-Voragen and Gilbert (2001) Biochem. J. 358, 607-614] the chromosome of Pseudomonas cellulosa was shown to contain two genes, abf51A and...
Beylot, M H, McKie, V A, Voragen, A G, Doeswijk-Voragen, C H, Gilbert, H J
To investigate the mechanism by which Pseudomonas cellulosa releases arabinose from polysaccharides and oligosaccharides, a gene library of P. cellulosa genomic DNA was screened for...
Role of hydrogen bonding in the interaction between a xylan binding module and xylan
Xie, H.F., Bolam, D.N., Nagy, T., Szabo, L., Cooper, A., Simpson, P.J., ...
NMR studies of the internal family 2b carbohydrate binding module (CBM2b-1) of Cellulomonas fimi xylanase 11A have identified six polar residues and two aromatic residues that interact with its...