Tanaka, S., Yamada, K., Yabumoto, K., Fujii, S., Huser, A., Tsuji, G., ...
Trends in antimicrobial resistance of Streptococcus pneumoniae in Japan.
Yoshida, R, Kaku, M, Kohno, S, Ishida, K, Mizukane, R, Takemura, H, ...
A total of 184 isolates of Streptococcus pneumoniae were recovered from the sputa of patients over a 5-year period in the Nagasaki area and were examined. A total of 30 strains were resistant to...
Ohno, H, Koga, H, Kohno, S, Tashiro, T, Hara, K
We analyzed the relationship between rifampin MICs and rpoB mutations of 40 clinical isolates of Mycobacterium tuberculosis. A point mutation in either codon 516, 526, or 531 was found in 13 strains...
Takemura, H, Kaku, M, Kohno, S, Hirakata, Y, Tanaka, H, Yoshida, R, ...
Defensins are small cationic bactericidal peptides present abundantly in the granules of polymorphonuclear neutrophils (PMNs). Human PMNs contain four defensins termed HNP-1 to HNP-4. We used a new...
Sawai, T, Tomono, K, Yanagihara, K, Yamamoto, Y, Kaku, M, Hirakata, Y, ...
We describe a novel mouse model of acute staphylococcal pneumonia induced by intravenous injection of Staphylococcus aureus enmeshed in agar beads. For comparison, we also used various strains of...
Kakeya, H, Udono, H, Ikuno, N, Yamamoto, Y, Mitsutake, K, Miyazaki, T, ...
Heat shock proteins (HSPs) from several pathogenic microbes have been shown to be target molecules of humoral responses as well as cellular immune responses. However, little is known about target...
High-performance liquid chromatography was used to determine the penetration of 19 antimicrobial agents into human polymorphonuclear leukocytes. The ratios of the intracellular concentration to the...
Efficacy of NY-198 against experimental Legionnaires disease.
Kohno, S, Yamaguchi, K, Dohtsu, Y, Koga, H, Hayashi, T, Hirota, M, ...
The in vitro and in vivo effects of NY-198 against Legionella pneumophila were compared with those of ciprofloxacin. The MIC of NY-198 against 15 standard reference strains of Legionella of various...
Saito, A, Sawatari, K, Fukuda, Y, Nagasawa, M, Koga, H, Tomonaga, A, ...
The antimicrobial activity of ofloxacin was tested against 15 standard strains and 37 clinical and environmental strains of Legionella pneumophila by agar dilution susceptibility studies with a new...
Accumulation of a newly developed fluoroquinolone, OPC-17116, by human polymorphonuclear leukocytes.
The accumulation of OPC-17116 by human polymorphonuclear leukocytes was studied by comparison with three other new quinolones, ofloxacin, levofloxacin, and DR-3354. The intracellular/extracellular...
Maesaki, S, Kohno, S, Kaku, M, Koga, H, Hara, K
The in vitro effects of antifungal agent combinations administered simultaneously and sequentially against 15 strains of Aspergillus fumigatus were tested by the yeast-malt broth method. The...
Efficacy of intravenous itraconazole against experimental pulmonary aspergillosis.
Miyazaki, H M, Kohno, S, Miyazaki, Y, Mitsutake, K, Tomono, K, Kaku, M, ...
The efficacy of intravenous itraconazole solubilized in hydroxypropyl-beta-cyclodextrin was assessed in a rat model of Aspergillus fumigatus pneumonia. Immunosuppressed rats were infected by...
camR, a negative regulator locus of the cytochrome P-450cam hydroxylase operon.
Koga, H, Aramaki, H, Yamaguchi, E, Takeuchi, K, Horiuchi, T, Gunsalus, I C
A 4.27-kilobase insert from a HindIII DNA library of Pseudomonas putida carrying the CAM plasmid allowed coordinate expression of genes camD and camC under control of camR, an upstream regulator. The...
Yamamoto, Y, Kohno, S, Koga, H, Kakeya, H, Tomono, K, Kaku, M, ...
We examined clinical (pulmonary cryptococcosis and cryptococcal meningitis) and environmental (pigeon excreta) isolates of Cryptococcus neoformans var. neoformans (serotype A) in the southern...
Miyamoto, J, Koga, H, Kohno, S, Tashiro, T, Hara, K
We developed a novel method for early detection of drug-resistant strains of Mycobacterium tuberculosis by using the hybridization protection assay (HPA). The number of viable bacteria during the...
Detection of Bacteroides fragilis in clinical specimens by PCR.
Yamashita, Y, Kohno, S, Koga, H, Tomono, K, Kaku, M
The direct detection of Bacteroides fragilis from clinical specimens was examined by using the PCR method for amplifying a specific fragment of the glutamine synthetase gene from B. fragilis. By this...
Rapid and highly reproducible method for antifungal susceptibility testing of Aspergillus species.
Yamada, H, Kohno, S, Maesaki, S, Koga, H, Kaku, M, Hara, K, ...
A new method for determining the MICs for Aspergillus species with slant-shaking incubation is described. The method was rapid (24 h), produced sharp visual end points, and showed high...
Pneumonia caused by Stenotrophomonas maltophilia with a mucoid phenotype.
Irifune, K, Ishida, T, Shimoguchi, K, Ohtake, J, Tanaka, T, Morikawa, N, ...
We describe the first known case of pneumonia caused by a mucoid Stenotrophomonas maltophilia (Xanthomonas maltophilia) strain in a patient with bronchiectasis. The patient was admitted because of...
Matsuda, H, Kohno, S, Maesaki, S, Yamada, H, Koga, H, Tamura, M, ...
The ubiquinone systems and electrophoretic comparison of enzymes were used to determine the relatedness among 64 isolates of seven Aspergillus spp. These were 31 clinical and 3 nonclinical isolates...
Nested polymerase chain reaction for detection of Mycobacterium tuberculosis in clinical samples.
Miyazaki, Y, Koga, H, Kohno, S, Kaku, M
The nested polymerase chain reaction technique was compared with the conventional smear and culture methods for detection of Mycobacterium tuberculosis. The nested polymerase chain reaction used in...
Mizukane, R, Hirakata, Y, Kaku, M, Ishii, Y, Furuya, N, Ishida, K, ...
The inhibitory effects of azithromycin (AZM), a new 15-membered macrolide antibiotic, on the production of exotoxin A, total protease, elastase, and phospholipase C by Pseudomonas aeruginosa were...
Imai, M, Shimada, H, Watanabe, Y, Matsushima-Hibiya, Y, Makino, R, Koga, H, ...
Site-directed mutants of cytochrome P-450cam (the cytochrome P-450 that acts as the terminal monooxygenase in the d-camphor monooxygenase system), in which threonine-252 had been changed to alanine,...
Genetic mapping of ossification of the posterior longitudinal ligament of the spine.
Koga, H, Sakou, T, Taketomi, E, Hayashi, K, Numasawa, T, Harata, S, ...
Ossification of the posterior longitudinal ligament of the spine (OPLL) is recognized as a common disorder among Japanese and throughout Asia. Estimates of its prevalence are in the range of 1....
Trends in antimicrobial resistance of Streptococcus pneumoniae in Japan.
Yoshida, R, Kaku, M, Kohno, S, Ishida, K, Mizukane, R, Takemura, H, ...
A total of 184 isolates of Streptococcus pneumoniae were recovered from the sputa of patients over a 5-year period in the Nagasaki area and were examined. A total of 30 strains were resistant to...
Ohno, H, Koga, H, Kohno, S, Tashiro, T, Hara, K
We analyzed the relationship between rifampin MICs and rpoB mutations of 40 clinical isolates of Mycobacterium tuberculosis. A point mutation in either codon 516, 526, or 531 was found in 13 strains...
Takemura, H, Kaku, M, Kohno, S, Hirakata, Y, Tanaka, H, Yoshida, R, ...
Defensins are small cationic bactericidal peptides present abundantly in the granules of polymorphonuclear neutrophils (PMNs). Human PMNs contain four defensins termed HNP-1 to HNP-4. We used a new...
Sawai, T, Tomono, K, Yanagihara, K, Yamamoto, Y, Kaku, M, Hirakata, Y, ...
We describe a novel mouse model of acute staphylococcal pneumonia induced by intravenous injection of Staphylococcus aureus enmeshed in agar beads. For comparison, we also used various strains of...
Kakeya, H, Udono, H, Ikuno, N, Yamamoto, Y, Mitsutake, K, Miyazaki, T, ...
Heat shock proteins (HSPs) from several pathogenic microbes have been shown to be target molecules of humoral responses as well as cellular immune responses. However, little is known about target...
High-performance liquid chromatography was used to determine the penetration of 19 antimicrobial agents into human polymorphonuclear leukocytes. The ratios of the intracellular concentration to the...
Efficacy of NY-198 against experimental Legionnaires disease.
Kohno, S, Yamaguchi, K, Dohtsu, Y, Koga, H, Hayashi, T, Hirota, M, ...
The in vitro and in vivo effects of NY-198 against Legionella pneumophila were compared with those of ciprofloxacin. The MIC of NY-198 against 15 standard reference strains of Legionella of various...
Saito, A, Sawatari, K, Fukuda, Y, Nagasawa, M, Koga, H, Tomonaga, A, ...
The antimicrobial activity of ofloxacin was tested against 15 standard strains and 37 clinical and environmental strains of Legionella pneumophila by agar dilution susceptibility studies with a new...
Accumulation of a newly developed fluoroquinolone, OPC-17116, by human polymorphonuclear leukocytes.
The accumulation of OPC-17116 by human polymorphonuclear leukocytes was studied by comparison with three other new quinolones, ofloxacin, levofloxacin, and DR-3354. The intracellular/extracellular...
Maesaki, S, Kohno, S, Kaku, M, Koga, H, Hara, K
The in vitro effects of antifungal agent combinations administered simultaneously and sequentially against 15 strains of Aspergillus fumigatus were tested by the yeast-malt broth method. The...
Efficacy of intravenous itraconazole against experimental pulmonary aspergillosis.
Miyazaki, H M, Kohno, S, Miyazaki, Y, Mitsutake, K, Tomono, K, Kaku, M, ...
The efficacy of intravenous itraconazole solubilized in hydroxypropyl-beta-cyclodextrin was assessed in a rat model of Aspergillus fumigatus pneumonia. Immunosuppressed rats were infected by...
camR, a negative regulator locus of the cytochrome P-450cam hydroxylase operon.
Koga, H, Aramaki, H, Yamaguchi, E, Takeuchi, K, Horiuchi, T, Gunsalus, I C
A 4.27-kilobase insert from a HindIII DNA library of Pseudomonas putida carrying the CAM plasmid allowed coordinate expression of genes camD and camC under control of camR, an upstream regulator. The...
Yamamoto, Y, Kohno, S, Koga, H, Kakeya, H, Tomono, K, Kaku, M, ...
We examined clinical (pulmonary cryptococcosis and cryptococcal meningitis) and environmental (pigeon excreta) isolates of Cryptococcus neoformans var. neoformans (serotype A) in the southern...
Miyamoto, J, Koga, H, Kohno, S, Tashiro, T, Hara, K
We developed a novel method for early detection of drug-resistant strains of Mycobacterium tuberculosis by using the hybridization protection assay (HPA). The number of viable bacteria during the...
Detection of Bacteroides fragilis in clinical specimens by PCR.
Yamashita, Y, Kohno, S, Koga, H, Tomono, K, Kaku, M
The direct detection of Bacteroides fragilis from clinical specimens was examined by using the PCR method for amplifying a specific fragment of the glutamine synthetase gene from B. fragilis. By this...
Rapid and highly reproducible method for antifungal susceptibility testing of Aspergillus species.
Yamada, H, Kohno, S, Maesaki, S, Koga, H, Kaku, M, Hara, K, ...
A new method for determining the MICs for Aspergillus species with slant-shaking incubation is described. The method was rapid (24 h), produced sharp visual end points, and showed high...
Pneumonia caused by Stenotrophomonas maltophilia with a mucoid phenotype.
Irifune, K, Ishida, T, Shimoguchi, K, Ohtake, J, Tanaka, T, Morikawa, N, ...
We describe the first known case of pneumonia caused by a mucoid Stenotrophomonas maltophilia (Xanthomonas maltophilia) strain in a patient with bronchiectasis. The patient was admitted because of...
Matsuda, H, Kohno, S, Maesaki, S, Yamada, H, Koga, H, Tamura, M, ...
The ubiquinone systems and electrophoretic comparison of enzymes were used to determine the relatedness among 64 isolates of seven Aspergillus spp. These were 31 clinical and 3 nonclinical isolates...
Nested polymerase chain reaction for detection of Mycobacterium tuberculosis in clinical samples.
Miyazaki, Y, Koga, H, Kohno, S, Kaku, M
The nested polymerase chain reaction technique was compared with the conventional smear and culture methods for detection of Mycobacterium tuberculosis. The nested polymerase chain reaction used in...
Mizukane, R, Hirakata, Y, Kaku, M, Ishii, Y, Furuya, N, Ishida, K, ...
The inhibitory effects of azithromycin (AZM), a new 15-membered macrolide antibiotic, on the production of exotoxin A, total protease, elastase, and phospholipase C by Pseudomonas aeruginosa were...
Imai, M, Shimada, H, Watanabe, Y, Matsushima-Hibiya, Y, Makino, R, Koga, H, ...
Site-directed mutants of cytochrome P-450cam (the cytochrome P-450 that acts as the terminal monooxygenase in the d-camphor monooxygenase system), in which threonine-252 had been changed to alanine,...
Genetic mapping of ossification of the posterior longitudinal ligament of the spine.
Koga, H, Sakou, T, Taketomi, E, Hayashi, K, Numasawa, T, Harata, S, ...
Ossification of the posterior longitudinal ligament of the spine (OPLL) is recognized as a common disorder among Japanese and throughout Asia. Estimates of its prevalence are in the range of 1....
Are DISH and OPLL genetically related?
HAVELKA, S, VESELA, M, PAVELKOVA, A, RUZICKOVA, S, KOGA, H, MAEDA, S, ...
Matsumoto, T, Iida, M, Kuroki, F, Hizawa, K, Koga, H, Fujishima, M
The effects of dietary pretreatment on longitudinal ulcers of the intestine induced by indomethacin given intracolonically were investigated in rats. The rats were pretreated with either standard...
Decreased plasma tryptophan associated with deep white matter lesions in elderly subjects
Yao, H, Yuzuriha, T, Koga, H, Fukuda, K, Endo, K, Matsumoto, T, ...
The aim was to identify potentially treatable risk factors for cerebral white matter lesions often found on MRI in elderly persons. findings were assessed on 1.0 T MRI of 178 subjects living in the...
Quantitative MRI findings and cognitive impairment among community dwelling elderly subjects
Koga, H, Yuzuriha, T, Yao, H, Endo, K, Hiejima, S, Takashima, Y, ...
Objectives: To study the factors which influence cognitive impairment among elderly subjects living in a local community, based on both MRI and clinical findings, to further elucidate the causes of...