The primary structure of chicken B-creatine kinase and evidence for heterogeneity of its mRNA (1986)
Hossle, J.P., Rosenberg, U.B., Schäfer, B., Eppenberger, H.M.
cDNA clones for chicken B-CK were isolated by immunoscreening from a gizzard cDNA library constructed in the expression vector λgtll. The entire coding portion in addition to the complete 3′...
Donath, M Y, Zapf, J, Eppenberger-Eberhardt, M, Froesch, E R, Eppenberger, H M
Adult rat cardiomyocytes in long-term culture express type 1 insulin-like growth factor (IGF) receptors. In contrast to insulin receptors, type 1 IGF receptors are up-regulated during culturing....
Stadhouders, A M, Jap, P H, Winkler, H P, Eppenberger, H M, Wallimann, T
Overaccumulation of abnormally organized mitochondria in so-called "ragged-red" skeletal muscle fibers is a morphological hallmark of mitochondrial myopathies, in particular of mitochondrial...
Biogenesis of the nuclear lamina: in vivo synthesis and processing of nuclear protein precursors.
Lehner, C F, Fürstenberger, G, Eppenberger, H M, Nigg, E A
Utilizing antibodies against lamins A, B1, and B2, we have studied the biogenesis of the nuclear lamina in chicken embryo fibroblasts. (Lamins B1 and B2 have been identified recently as structurally...
Wallimann, T, Wegmann, G, Moser, H, Huber, R, Eppenberger, H M
Two isoforms of creatine kinase (CK; ATP:creatine N-phosphotransferase, E.C. 2.7.3.2), brain type (BB-CK) and mitochondrial type (MiMi-CK), but not the muscle types (MM- or hybrid MB-CK), were...
Monovalent antibodies against MM-creatine kinase remove the M line from myofibrils.
Wallimann, T, Pelloni, G, Turner, D C, Eppenberger, H M
Column-purified antibodies against creatine kinase (EC 2.7.3.2) from chicken skeletal muscle (the homodimeric isoenzyme designated MM-CK) bind specifically to the M lines of chicken pectoral muscle...
The primary structure of chicken B-creatine kinase and evidence for heterogeneity of its mRNA.
Hossle, J P, Rosenberg, U B, Schäfer, B, Eppenberger, H M, Perriard, J C
cDNA clones for chicken B-CK were isolated by immunoscreening from a gizzard cDNA library constructed in the expression vector lambda gtll. The entire coding portion in addition to the complete 3'...
Molecular cloning and expression during myogenesis of sequences coding for M-creatine kinase.
Rosenberg, U B, Kunz, G, Frischauf, A, Lehrach, H, Mähr, R, Eppenberger, H M, ...
Sequences complementary to muscle poly(A)+RNA were cloned in the plasmid pBR322 and the resulting colonies were screened by colony hybridization with labeled cDNA derived from skeletal muscle and...
Birchmeier, C, Kreis, T E, Eppenberger, H M, Winterhalter, K H, Birchmeier, W
The distributions of both fibronectin (LETS, CSP) fibers and focal contacts to the substratum, as viewed by fluorescence and reflection contrast microscopy, respectively, have been compared in...
Gosteli-Peter, M A, Harder, B A, Eppenberger, H M, Zapf, J, Schaub, M C
Effects of triiodothyronine (T3) on the expression of cytoskeletal and myofibrillar proteins in adult rat cardiomyocytes (ARC) were followed during two weeks of culture in the presence of 20%...
Nigg, E A, Hilz, H, Eppenberger, H M, Dutly, F
In unstimulated interphase bovine epithelial (MDBK) cells, both regulatory (R II) and catalytic (C) subunits of the type II enzyme of cAMP-dependent protein kinase (cAMP-dPK II) are associated with...
Unshadowed myosin molecules: STEM mass-maps of myosin heads.
Walzthöny, D, Bähler, M, Eppenberger, H M, Wallimann, T, Engel, A
Myosin molecules were directly visualized without heavy metal shadowing by scanning transmission electron microscopy (STEM) under low dose conditions. The general appearance and dimensions of heavy...
Expression of active octameric chicken cardiac mitochondrial creatine kinase in Escherichia coli.
Furter, R, Kaldis, P, Furter-Graves, E M, Schnyder, T, Eppenberger, H M, Wallimann, T
Sarcomeric mitochondrial creatine kinase (Mib-CK) of chicken was expressed in Escherichia coli as a soluble enzyme by using an inducible phage-T7 promoter. Up to one third of the protein in E. coli...
Donath, M Y, Zapf, J, Eppenberger-Eberhardt, M, Froesch, E R, Eppenberger, H M
Adult rat cardiomyocytes in long-term culture express type 1 insulin-like growth factor (IGF) receptors. In contrast to insulin receptors, type 1 IGF receptors are up-regulated during culturing....
Stadhouders, A M, Jap, P H, Winkler, H P, Eppenberger, H M, Wallimann, T
Overaccumulation of abnormally organized mitochondria in so-called "ragged-red" skeletal muscle fibers is a morphological hallmark of mitochondrial myopathies, in particular of mitochondrial...
Biogenesis of the nuclear lamina: in vivo synthesis and processing of nuclear protein precursors.
Lehner, C F, Fürstenberger, G, Eppenberger, H M, Nigg, E A
Utilizing antibodies against lamins A, B1, and B2, we have studied the biogenesis of the nuclear lamina in chicken embryo fibroblasts. (Lamins B1 and B2 have been identified recently as structurally...
Wallimann, T, Wegmann, G, Moser, H, Huber, R, Eppenberger, H M
Two isoforms of creatine kinase (CK; ATP:creatine N-phosphotransferase, E.C. 2.7.3.2), brain type (BB-CK) and mitochondrial type (MiMi-CK), but not the muscle types (MM- or hybrid MB-CK), were...
Monovalent antibodies against MM-creatine kinase remove the M line from myofibrils.
Wallimann, T, Pelloni, G, Turner, D C, Eppenberger, H M
Column-purified antibodies against creatine kinase (EC 2.7.3.2) from chicken skeletal muscle (the homodimeric isoenzyme designated MM-CK) bind specifically to the M lines of chicken pectoral muscle...
The primary structure of chicken B-creatine kinase and evidence for heterogeneity of its mRNA.
Hossle, J P, Rosenberg, U B, Schäfer, B, Eppenberger, H M, Perriard, J C
cDNA clones for chicken B-CK were isolated by immunoscreening from a gizzard cDNA library constructed in the expression vector lambda gtll. The entire coding portion in addition to the complete 3'...
Molecular cloning and expression during myogenesis of sequences coding for M-creatine kinase.
Rosenberg, U B, Kunz, G, Frischauf, A, Lehrach, H, Mähr, R, Eppenberger, H M, ...
Sequences complementary to muscle poly(A)+RNA were cloned in the plasmid pBR322 and the resulting colonies were screened by colony hybridization with labeled cDNA derived from skeletal muscle and...
Birchmeier, C, Kreis, T E, Eppenberger, H M, Winterhalter, K H, Birchmeier, W
The distributions of both fibronectin (LETS, CSP) fibers and focal contacts to the substratum, as viewed by fluorescence and reflection contrast microscopy, respectively, have been compared in...
Gosteli-Peter, M A, Harder, B A, Eppenberger, H M, Zapf, J, Schaub, M C
Effects of triiodothyronine (T3) on the expression of cytoskeletal and myofibrillar proteins in adult rat cardiomyocytes (ARC) were followed during two weeks of culture in the presence of 20%...
Nigg, E A, Hilz, H, Eppenberger, H M, Dutly, F
In unstimulated interphase bovine epithelial (MDBK) cells, both regulatory (R II) and catalytic (C) subunits of the type II enzyme of cAMP-dependent protein kinase (cAMP-dPK II) are associated with...
Unshadowed myosin molecules: STEM mass-maps of myosin heads.
Walzthöny, D, Bähler, M, Eppenberger, H M, Wallimann, T, Engel, A
Myosin molecules were directly visualized without heavy metal shadowing by scanning transmission electron microscopy (STEM) under low dose conditions. The general appearance and dimensions of heavy...
Expression of active octameric chicken cardiac mitochondrial creatine kinase in Escherichia coli.
Furter, R, Kaldis, P, Furter-Graves, E M, Schnyder, T, Eppenberger, H M, Wallimann, T
Sarcomeric mitochondrial creatine kinase (Mib-CK) of chicken was expressed in Escherichia coli as a soluble enzyme by using an inducible phage-T7 promoter. Up to one third of the protein in E. coli...
Gross, M., Furter-Graves, E. M., Wallimann, T., Eppenberger, H. M., Furter, R.
The 5 tryptophan residues of chicken sarcomeric mitochondrial creatine kinase (Mib-CK) were individually replaced by phenylalanine or cysteine using site-directed mutagenesis. The mutant proteins...