Lupus nephritis: treatment with mycophenolate mofetil (2004)
Kapitsinou, P. P., Boletis, J. N., Skopouli, F. N., Boki, K. A., Moutsopoulos, H. M.
Objective. To evaluate the safety and efficacy of mycophenolate mofetil (MMF) treatment in patients with lupus nephritis. Methods. Eighteen patients with biopsy-proven lupus nephritis (17 females,...
Autoimmune phenomena in myelodysplastic syndromes: a 4-yr prospective study (2004)
Giannouli, S., Voulgarelis, M., Zintzaras, E., Tzioufas, A. G., Moutsopoulos, H. M.
Objective. To determine the clinical aspects and evolution of autoimmune inflammatory manifestations (AIMs) in patients with myelodysplastic syndrome (MDS) and ascertain the prognostic implications...
Lupus nephritis: treatment with mycophenolate mofetil (2004)
Kapitsinou, P. P., Boletis, J. N., Skopouli, F. N., Boki, K. A., Moutsopoulos, H. M.
Objective. To evaluate the safety and efficacy of mycophenolate mofetil (MMF) treatment in patients with lupus nephritis. Methods. Eighteen patients with biopsy-proven lupus nephritis (17 females,...
Autoimmune phenomena in myelodysplastic syndromes: a 4-yr prospective study (2004)
Giannouli, S., Voulgarelis, M., Zintzaras, E., Tzioufas, A. G., Moutsopoulos, H. M.
Objective. To determine the clinical aspects and evolution of autoimmune inflammatory manifestations (AIMs) in patients with myelodysplastic syndrome (MDS) and ascertain the prognostic implications...
Lupus nephritis: treatment with mycophenolate mofetil (2004)
Kapitsinou, P. P., Boletis, J. N., Skopouli, F. N., Boki, K. A., Moutsopoulos, H. M.
Objective. To evaluate the safety and efficacy of mycophenolate mofetil (MMF) treatment in patients with lupus nephritis. Methods. Eighteen patients with biopsy-proven lupus nephritis (17 females,...
Autoimmune phenomena in myelodysplastic syndromes: a 4-yr prospective study (2004)
Giannouli, S., Voulgarelis, M., Zintzaras, E., Tzioufas, A. G., Moutsopoulos, H. M.
Objective. To determine the clinical aspects and evolution of autoimmune inflammatory manifestations (AIMs) in patients with myelodysplastic syndrome (MDS) and ascertain the prognostic implications...
Ioannidis, J. P. A., Katsifis, G. E., Stavropoulos, E. D., Manoussakis, M. N., Moutsopoulos, H. M.
Objective. To evaluate whether autoantibodies in the absence of rheumatic diseases increase the risk of mortality among very elderly subjects who are otherwise in good functional condition. Methods....
Vlachoyiannopoulos, P. G., Kanellopoulos, P. G., Ioannidis, J. P. A., Tektonidou, M. G., Mastorakou, I., Moutsopoulos, H. M.
Objective. To evaluate whether premenopausal women with antiphospholipid syndrome (APS) or systemic lupus erythematosus (SLE) have increased prevalence of atherosclerosis after...
Katsifis, G. E., Tzioufas, A. G., Vlachoyiannopoulos, P. G., Voulgarelis, M., Moutsopoulos, H. M., Ioannidis, J. P. A.
Objectives. To determine the incidence of serious myelotoxicity from intravenous cyclophosphamide (IC) in systemic lupus erythematosus (SLE). Methods. In a retrospective...
Swaak, A.J., Moutsopoulos, H.M., Dratwianka, B., Kalden, J.R., Tosi, S., Marchesoni, A., ...
OBJECTIVE: Patients characterized with antinuclear antibodies (ANA) and disease symptoms related to one organ system can be described as having incomplete systemic lupus erythematosus (SLE). The aim...
Renal involvement in antiphospholipid syndrome (2001)
Vlachoyiannopoulos, P. G., Kanellopoulos, P., Tektonidou, M., Moutsopoulos, H. M.
Swaak, A. J. G., Smeenk, R. J. T., Manger, K., Kalden, J. R., Tosi, S., ...
Objective. Patients characterized with antinuclear antibodies (ANA) and disease symptoms related to one organ system can be described as having incomplete systemic lupus erythematosus...
Prognostic factors and clustering of serious clinical outcomes in antiphospholipid syndrome (2000)
Tektonidou, M.G., Ioannidis, J.P.A., Boki, K.A., Vlachoyiannopoulos, P.G., Moutsopoulos, H.M.
We assessed whether initial clinical presentations suggestive of antiphospholipid syndrome (APS) predicted the subsequent rate and type of serious clinical outcomes. Eighty‐two...
Risk factors for central nervous system involvement in systemic lupus erythematosus (2000)
KARASSA, F.B., IOANNIDIS, J.P.A., TOULOUMI, G., BOKI, K.A., MOUTSOPOULOS, H.M.
We investigated risk factors for central nervous system (CNS) involvement in systemic lupus erythematosus (SLE), in 32 such patients individually matched 1 : 3 to 96...
Swaak, A.J., Rozman, B., Chwalinska-Sadowska, H, Bombardieri, S., Kovacs, A., Schneider, M., ...
OBJECTIVE: Most information available about the disease course of patients with systemic lupus erythematosus (SLE) is restricted to the first 5 yr after disease onset. Data about the disease course...
Microvascular abnormalities in Sjogren's syndrome: nailfold capillaroscopy (1999)
Tektonidou, M., Kaskani, E., Skopouli, F. N., Moutsopoulos, H. M.
Objective. To describe microvascular abnormalities by nailfold capillaroscopy in patients with primary Sjögren's syndrome (SS) with or without Raynaud's phenomenon (RP) and those with anticentromere...
Recurrent pregnancy loss and autoantibody profile in autoimmune diseases (1999)
Mavragani, C. P., Ioannidis, J. P. A., Tzioufas, A. G., Hantoumi, I. E., Moutsopoulos, H. M.
Objective. To explore the association of non-organ-specific autoimmune responses against three distinct Ro antigen-related reactivities (Ro52, Ro60, p57) with a history of pregnancy loss in women...
Swaak, A. J. G., Van Den Brink, H. G., Smeenk, R. J. T., Manger, K., Kalden, J. R., Tosi, S., ...
Objective. Most information available about the disease course of patients with systemic lupus erythematosus (SLE) is restricted to the first 5 yr after disease onset. Data about the disease course...
PREDOMINANCE OF IgM ANTI-U1RNP ANTIBODIES IN PATIENTS WITH SYSTEMIC LUPUS ERYTHEMATOSUS (1996)
VLACHOYIANNOPOULOS, P. G., GUIALIS, A., TZIOUFAS, A. G., MOUTSOPOULOS, H. M.
Anti-UIRNP antibodies occur in patients with mixed connective tissue disease (MCTD), systemic sclerosis (SSc), systemic lupus erythematosus (SLE) and other ill-defined connective tissue diseases. To...
BOUMBA, D., SKOPOULI, F. N., MOUTSOPOULOS, H. M.
The pattern of cytokine mRNA expression in frozen minor salivary gland tissues from patients with primary Sjögren's syndrome (pSS) (n = 12) and controls (n = 8) using an in situ...
LIVER INVOLVEMENT IN PRIMARY SJOGREN'S SYNDROME (1994)
SKOPOULI, F. N., BARBATIS, C., MOUTSOPOULOS, H. M.
Three hundred patients with primary Sjögren's syndrome (pSS) were investigated for liver involvement using clinical, biochemical, immunological and histological data. Seven percent of patients...
DETECTION OF ANTI-Ro(SSA) ANTIBODIES IN AUTOIMMUNE DISEASES: COMPARISON OF FIVE METHODS (1993)
MANOUSSAKIS, M. N., KISTIS, K. G., LIU, X., AIDINIS, V., GUIALIS, A., MOUTSOPOULOS, H. M.
Counterimmunoelectrophoresis (CIE), RNA precipitation, ELISA and immunoblotting against cytoplasmic HeLa cell extract (IB-HeLa) and erythrocyte extract (IB-RBC) were applied to detect anti-Ro(SSA)...
PATIENTS WITH ANTICENTROMERE ANTIBODIES, CLINICAL FEATURES, DIAGNOSES AND EVOLUTION (1993)
VLACHOYIANNOPOULOS, P. G., DROSOS, A. A., WIIK, A., MOUTSOPOULOS, H. M.
The sera of 1373 patients derived from a random population of 2627 individuals examined in a rheumatology outpatient clinic were found to be positive for antinuclear antibodies (ANA). Forty-seven of...
THE SPECTRUM OF NEUROLOGICAL INVOLVEMENT IN SJOGREN'S SYNDROME (1990)
ANDONOPOULOS, A. P., LAGOS, G., DROSOS, A. A., MOUTSOPOULOS, H. M.
Sixty-three unselected consecutive patients with primary Sjögren's syndrome (pSs) were prospectivelyevaluated for evidence of neurological manifestations. Seventeen had a mild sensory or mixed...
PREVALENCE OF PRIMARY SJOGREN'S SYNDROME IN AN ELDERLY POPULATION (1988)
DROSOS, A. A., ANDONOPOULOS, A. P., COSTOPOULOS, J. S., PAPADIMITRIO, C. S., MOUTSOPOULOS, H. M.
Sixty-two elderly apparently healthy volunteers, inmates of a public nursing home, were examined for evidence of primary Sjogren's syndrome (pSS). Evaluation included a specific questionnaire for...
Dangli, A, Plomaritoglou, A, Boutou, E, Vassiliadou, N, Moutsopoulos, H M, Guialis, A
The structurally related A/B-type core heterogeneous nuclear ribonucleoprotein (hnRNP) polypeptides of 34-39 kDa (A1, A2, B1 and B2) belong to a family of RNA-binding proteins that are major...
Dangli, A, Plomaritoglou, A, Boutou, E, Vassiliadou, N, Moutsopoulos, H M, Guialis, A
The structurally related A/B-type core heterogeneous nuclear ribonucleoprotein (hnRNP) polypeptides of 34-39 kDa (A1, A2, B1 and B2) belong to a family of RNA-binding proteins that are major...
Asymptomatic diffuse fasciitis with eosinophilia
Avgerinos, P. C., Papadimitriou, C. S., Kokkini, G., Moutsopoulos, H. M.
The case of a 70-year-old woman with asymptomatic diffuse fasciitis with eosinophilia, confirmed by biopsy, is presented.
Selective renal angiography in Wegener's granulomatosis
Moutsopoulos, H. M., Avgerinos, P. C., Tsampoulas, C. G., Katsiotis, P. A.
Two patients whose illnesses meet the criteria of Wegener's granulomatosis are presented. Selective renal angiography performed in both revealed multiple arterial aneurysms similar to those...
HLA DR alloantigens in different subsets of patients with Sjögren's syndrome and in family members.
Patients with Sjögren's syndrome alone (Ss), Sjögren's syndrome with rheumatoid arthritis (Ss-RA), and Sjögren's syndrome with Raynaud's phenomenon (Ss-RP) were typed for the HLA DR and MT...
Moutsopoulos, H M, Giotaki, H, Maddison, P J, Mavridis, A C, Drosos, A A, Skopouli, F N
One hundred and twenty-four sera from Greek patients with autoimmune rheumatic diseases (29 with systemic lupus erythematosus (SLE), 24 with scleroderma, 11 with primary Sjögren's syndrome (SS), and...
Moutsopoulos, H M, Costello, R, Drosos, A A, Mavridis, A K, Papadopoulos, N M
Fresh sera and concentrated urine from 17 patients with primary Sjögren's syndrome (SS) were fractionated by high-resolution agarose electrophoresis to investigate the presence of monoclonal...
Moutsopoulos, H M, Skopouli, F N, Sarras, A K, Tsampoulas, C, Mavridis, A K, Constantopoulos, S H, ...
The clinical, laboratory, histological, and radiological manifestations of 90 Greek patients with anti-Ro(SSA) negative rheumatoid arthritis (RA) were compared with those of 15 Greek patients with...
Tsianos, E B, Tzioufas, A G, Mavridis, A K, Sarras, A K, Papadimitriou, C S, Tsolas, O, ...
Fifty-one patients with autoimmune rheumatic diseases underwent biopsy of the labial minor salivary glands. These patients were divided according to histopathology of lip biopsies into three groups...
Oesophageal dysfunction in patients with primary Sjögren's syndrome.
Tsianos, E B, Chiras, C D, Drosos, A A, Moutsopoulos, H M
Oesophageal motility was studied in 22 patients with primary Sjögren's syndrome and 20 normal volunteers. Oesophageal dysfunction was detected in eight of the 22 patients (36.4%) with primary...
HLA-DR expression by labial minor salivary gland tissues in Sjögren's syndrome.
Moutsopoulos, H M, Hooks, J J, Chan, C C, Dalavanga, Y A, Skopouli, F N, Detrick, B
Minor salivary gland biopsy specimens from patients with Sjögren's syndrome (primary and secondary) and from normal controls were examined with the four step biotin-avidin-immunoperoxidase assay....
Cyclosporin A (CyA) in primary Sjögren's syndrome: a double blind study.
Drosos, A A, Skopouli, F N, Costopoulos, J S, Papadimitriou, C S, Moutsopoulos, H M
The efficacy and toxicity of cyclosporin A (CyA) were studied in a blind fashion in 20 patients with primary Sjögren's syndrome (pSS). The dose of CyA or placebo was 5 mg/kg of body weight daily....
Dalavanga, Y A, Detrick, B, Hooks, J J, Drosos, A A, Moutsopoulos, H M
Labial minor salivary gland biopsy specimens from 14 patients with Sjögren's syndrome treated either with cyclosporin A (CyA) or placebo (5 mg/kg body weight day for six months) were studied to...
Outcome of pregnancy in patients with autoimmune rheumatic disease before the disease onset.
Siamopoulou-Mavridou, A, Manoussakis, M N, Mavridis, A K, Moutsopoulos, H M
The outcomes of 419 pregnancies of 154 unselected patients with various auto-immune diseases, including 390 pregnancies before the disease onset, were studied retrospectively. The patients comprised...
Middle ear function in patients with juvenile chronic arthritis.
Siamopoulou-Mavridou, A, Asimakopoulos, D, Mavridis, A, Skevas, A, Moutsopoulos, H M
The conductive hearing of 18 patients with juvenile chronic arthritis was studied. Tympanometry was therefore undertaken for the assessment of functional state of the tympanic membrane, the ossicular...
Moutsopoulos, H M, Tzioufas, A G, Bai, M K, Papadopoulos, N M, Papadimitriou, C S
Minor salivary gland biopsy specimens from 11 patients with primary Sjögren's syndrome with circulating monoclonal IgM kappa cryoglobulins, seven without cryoglobulins, and four patients with...
Slow viruses and the immune system in the pathogenesis of local tissue damage in Sjögren's syndrome.
Examination of HLA-DR4 as a severity marker for rheumatoid arthritis in Greek patients.
Boki, K A, Drosos, A A, Tzioufas, A G, Lanchbury, J S, Panayi, G S, Moutsopoulos, H M
OBJECTIVES--Previous reports have shown that HLA-DR4 may be a severity marker for rheumatoid arthritis (RA) in patients of northern European origin. The aim of the present study was to investigate...
Obstetric and gynaecological profile in patients with primary Sjögren's syndrome.
Skopouli, F N, Papanikolaou, S, Malamou-Mitsi, V, Papanikolaou, N, Moutsopoulos, H M
OBJECTIVE--To describe the effects of Sjögren's syndrome (SS) on the fertility, parity and sexual activity as well as investigating the aetiopathology of dyspareunia in female patients....
Vitali, C, Moutsopoulos, H M, Bombardieri, S
AIM--To establish a definitive set of diagnostic criteria in a multicentre European study a selected number of oral and ocular tests were performed on a large number of patients with Sjögrens...
Vitali, C, Bombardieri, S, Moutsopoulos, H M, Coll, J, Gerli, R, Hatron, P Y, ...
OBJECTIVE: To assess the recently proposed preliminary criteria for the classification of Sjögren's syndrome (SS) in a multicentre European study of a new series of clinically defined cases....
Safety and efficacy of recombinant gamma interferon in the treatment of systemic sclerosis.
Vlachoyiannopoulos, P G, Tsifetaki, N, Dimitriou, I, Galaris, D, Papiris, S A, Moutsopoulos, H M
OBJECTIVE: To evaluate the safety and efficacy of recombinant gamma interferon (rIFN gamma) in the treatment of patients with systemic sclerosis. METHODS: Sixteen patients with systemic sclerosis...
Dalekos, G N, Manoussakis, M N, Goussia, A C, Tsianos, E V, Moutsopoulos, H M
Ulcerative colitis (UC) is an inflammatory bowel disease of unknown aetiology. In this study, serum samples from 80 patients with UC were studied for the presence of various autoantibodies and...
Routsias, J G, Sakarellos-Daitsiotis, M, Detsikas, E, Tzioufas, A G, Sakarellos, C, Moutsopoulos, H M
Previous studies demonstrated a possible antigenic relation between the carboxyl terminal portion of anti-Ro60kD autoantigen and a nucleocapsid protein (N) of vesicular stomatitis virus (VSV). In...
Guialis, A, Patrinou-Georgoula, M, Tsifetaki, N, Aidinis, V, Sekeris, C E, Moutsopoulos, H M
A patient with systemic lupus erythematosus (SLE) and nephritis without antibodies to dsDNA but with antibodies to a 5S RNA/protein (RNP) complex is presented. Combined RNA precipitation and Western...
Katsikis, P D, Youinou, P Y, Galanopoulou, V, Papadopoulos, N M, Tzioufas, A G, Moutsopoulos, H M
Monoclonal or oligoclonal B cell products have been described in the sera and urine of patients with primary Sjögren's syndrome (PSS). In addition, monoclonal expansion of plasma cells has been...
Shokri, F, Mageed, R A, Kitas, G D, Katsikis, P, Moutsopoulos, H M, Jefferis, R
The aetiology of sustained autoantibody production in human autoimmune diseases is unknown. Evidence for structural similarities and common clonal origin among autoantibodies have been demonstrated...
High prevalence of anti-cardiolipin and other autoantibodies in a healthy elderly population.
Manoussakis, M N, Tzioufas, A G, Silis, M P, Pange, P J, Goudevenos, J, Moutsopoulos, H M
Serum samples from 64 apparently healthy individuals (32 men and 32 women, mean age 81.0 years) were examined for the prevalence of several autoantibodies, including rheumatoid factor (RF),...
Calreticulin synthetic peptide analogues: anti-peptide antibodies in autoimmune rheumatic diseases.
Routsias, J G, Tzioufas, A G, Sakarellos-Daitsiotis, M, Sakarellos, C, Moutsopoulos, H M
Autoantibodies in sera from patients with systemic lupus erythematosus (SLE) and onchocerciasis recognize calreticulin (CaR), a calcium-binding protein, as antigen. In this study we present the...
STAIKOU, E V, ROUTSIAS, J G, MAKRI, A A, TERZOGLOU, A, SAKARELLOS-DAITSIOTIS, M, SAKARELLOS, C, ...
Calreticulin is a molecular chaperone to newly synthesized polypeptides. Previous studies suggested that calreticulin is probably a protein member of the Ro/La RNP complex. The aims of this study...
PAPAMATTHEOU, M G, ROUTSIAS, J G, KARAGOUNI, E E, SAKARELLOS, C, SAKARELLOS-DAITSIOTIS, M, MOUTSOPOULOS, H M, ...
Immunotherapies against autoimmune diseases have been of limited success. Preventive vaccines could be developed on the basis to abrogate unwanted immune responses to defined autodeterminants. In...
Stathopoulou, E A, Routsias, J G, Stea, E A, Moutsopoulos, H M, Tzioufas, A G
Coxsackie virus RNA has recently been detected in biopsy specimens of minor salivary glands from patients with primary Sjögren's Syndrome (pSS). A peptide derived from Coxsackie virus 2B protein...
Fine specificity of autoantibodies to La/SSB: epitope mapping, and characterization
TZIOUFAS, A G, YIANNAKI, E, SAKARELLOS-DAITSIOTIS, M, ROUTSIAS, J G, SAKARELLOS, C, MOUTSOPOULOS, H M
The B cell epitope mapping of La/SSB was performed using 20 mer synthetic peptides overlapping by eight amino acids covering the whole sequence of the protein. IgG, purified from sera of five...
Yiannaki, E E, Tzioufas, A G, Bachmann, M, Hantoumi, J, Tsikaris, V, Sakarellos-Daitsiotis, M, ...
In a previous study it was shown that La/SSB contains four linear epitopes, p147–154, p291–302, p301–318 and p349–364. The aim of the present study was to investigate the value of the...
Modes of epithelial cell death and repair in Sjögren's syndrome (SS)
POLIHRONIS, M, TAPINOS, N I, THEOCHARIS, S E, ECONOMOU, A, KITTAS, C, MOUTSOPOULOS, H M
We evaluated possible modes of epithelial cell destruction and restoration in minor salivary gland biopsies from patients with SS. Minor salivary gland biopsies from 10 primary Sjögren's syndrome...
CD4 cytotoxic and dendritic cells in the immunopathologic lesion of Sjögren's syndrome
Xanthou, G, Tapinos, N I, Polihronis, M, Nezis, I P, Margaritis, L H, Moutsopoulos, H M
The existence of CD4+ T lymphocytes with cytotoxic activity in minor salivary gland (MSG) biopsies from Sjögren's syndrome (SS) patients was investigated using in situ double immunohistochemistry...
Archimandritis, A, Sougioultzis, S, Foukas, P G, Tzivras, M, Davaris, P, Moutsopoulos, H M
There is evidence that Helicobacter pylori infection up-regulates the expression of HLA class II molecules by gastric epithelial cells (GEC). In this study we evaluated whether GEC are capable of...
Yiannaki, E, Vlachoyiannopoulos, P G, Manoussakis, M N, Sakarellos, C, Sakarellos-Daitsiotis, M, Moutsopoulos, H M, ...
The aim of this study was to investigate the immunogenicity of four synthetic peptides, representing linear B cell epitopes of the human La/SSB autoantigen: 145–164 aa (p1), 289–308 aa (p2),...
Kapsogeorgou, E K, Dimitriou, I D, Abu-Helu, R F, Moutsopoulos, H M, Manoussakis, M N
ICAM.1 (CD54) is a surface protein expressed on epithelial and other nonhematopoietic cells upon activation and is known to play an important role in the stimulation of T cells by the provision of...
MITSIAS, D I, TZIOUFAS, A G, VEIOPOULOU, C, ZINTZARAS, E, TASSIOS, I K, KOGOPOULOU, O, ...
Expression of type-1 and type-2 cytokines at the mRNA level in labial salivary glands (LSG) of patients with Sjogren’s syndrome (SS), as reported by several groups, have generated conflicting...
DIMITRIOU, I D, KAPSOGEORGOU, E K, MOUTSOPOULOS, H M, MANOUSSAKIS, M N
CD40 has been identified in an expanding list of haematopoietic and non-haematopoietic cells and has received an increased interest based on its role in a variety of cell-mediated responses and its...
Terzoglou, A G, Routsias, J G, Avrameas, S, Moutsopoulos, H M, Tzioufas, A G
Sera from patients with primary Sjögren Syndrome (pSS) or Systemic Lupus Erythematosus (SLE) often contain autoantibodies directed against La/SSB. The sequence 349–368aa represents the major...
B-cell epitopes of intracellular autoantigens: myth and reality.
Moutsopoulos, N. M., Routsias, J. G., Vlachoyiannopoulos, P. G., Tzioufas, A. G., Moutsopoulos, H. M.
Terzoglou, A G, Routsias, J G, Moutsopoulos, H M, Tzioufas, A G
Ro60 kDa is a member of the Ro/LaRNP ribonucleoprotein complex and its major linear B cell epitope, corresponding to the region 169–190aa, has been found to be the initial target of the autoimmune...
Stea, E A, Routsias, J G, Samiotaki, M, Panayotou, G, Papalambros, E, Moutsopoulos, H M, ...
Sjögren's syndrome (SS) is an autoimmune disease characterized by lymphocytic infiltration, destruction of the salivary and lacrimal glands and production of autoantibodies against a variety of...
Spachidou, M P, Bourazopoulou, E, Maratheftis, C I, Kapsogeorgou, E K, Moutsopoulos, H M, Tzioufas, A G, ...
Toll-like receptors (TLR) play an essential role in the activation of both innate and adaptive immune responses. Salivary gland epithelial cells (SGEC) may participate in the development of glandular...
Impaired elastic properties of ascending aorta in patients with giant cell arteritis
Margos, P N, Moyssakis, I E, Tzioufas, A G, Zintzaras, E, Moutsopoulos, H M