Scientific Commons: H. Mitsuya

Designing CD4 immunoadhesins for AIDS therapy (2007)

Capon, D. J., Chamow, S. M., Mordenti, J., Marsters, S. A., Gregory, T., Mitsuya, H., ...

A newly-constructed antibody-like molecule containing the gp120-binding domain of the receptor for human immunodeficiency virus blocks HIV-1 infection of T cells and monocytes. Its long plasma...

Disparate actions of hydroxyurea in potentiation of purine and pyrimidine 2',3'-dideoxynucleoside activities against replication of human immunodeficiency virus.

Gao, W Y, Johns, D G, Chokekuchai, S, Mitsuya, H

We and other groups have recently reported the potentiation by ribonucleotide reductase inhibitors such as hydroxyurea of the anti-human immunodeficiency virus type 1 (HIV-1) activity of purine and...

Changes in drug sensitivity of human immunodeficiency virus type 1 during therapy with azidothymidine, dideoxycytidine, and dideoxyinosine: an in vitro comparative study.

Shirasaka, T, Yarchoan, R, O'Brien, M C, Husson, R N, Anderson, B D, Kojima, E, ...

Human immunodeficiency virus type 1 (HIV-1) strains were isolated from nine patients before and after prolonged therapy with either an alternating regimen of 3'-azido-3'-deoxythymidine (AZT) and...

Lipophilic halogenated congeners of 2',3'-dideoxypurine nucleosides active against human immunodeficiency virus in vitro.

Shirasaka, T, Murakami, K, Ford, H, Kelley, J A, Yoshioka, H, Kojima, E, ...

Four 2-amino-6-halo- and four 6-halo-2',3'-dideoxypurine ribofuranosides (ddPs) were synthesized and tested for in vitro activity to suppress the infectivity, cytopathic effect, Gag protein...

Pharmacokinetics of dideoxypurine nucleoside analogs in plasma and cerebrospinal fluid of rhesus monkeys.

Hawkins, M E, Mitsuya, H, McCully, C M, Godwin, K S, Murakami, K, Poplack, D G, ...

The pharmacokinetics of 2',3'-dideoxyadenosine (ddA), didanosine, 2',3'-dideoxyguanosine (ddG), and 6-halogenated prodrugs of ddG, 6-chloro-ddG and 6-iodo-ddG, in plasma and cerebrospinal fluid (CSF)...

Comparative analysis of anti-human immunodeficiency virus type 1 activities of dideoxynucleoside analogs in resting and activated peripheral blood mononuclear cells.

Shirasaka, T, Chokekijchai, S, Yamada, A, Gosselin, G, Imbach, J L, Mitsuya, H

We determined the anti-human immunodeficiency virus type 1 (anti-HIV-1) activities of various dideoxy-nucleoside analogs by using phytohemagglutinin-activated peripheral blood mononuclear cells...

Removal of human immunodeficiency virus type 1 (HIV-1) protease inhibitors from preparations of immature HIV-1 virions does not result in an increase in infectivity or the appearance of mature morphology.

Humphrey, R W, Ohagen, A, Davis, D A, Fukazawa, T, Hayashi, H, Höglund, S, ...

The processing of gag and gag-pol polyproteins by human immunodeficiency virus type 1 (HIV-1) protease is a crucial step in the formation of infectious HIV-1 virions. In this study, we examine...

In vitro induction of human immunodeficiency virus type 1 variants resistant to 2'-beta-Fluoro-2',3'-dideoxyadenosine.

Tanaka, M, Srinivas, R V, Ueno, T, Kavlick, M F, Hui, F K, Fridland, A, ...

2'-beta-Fluoro-2',3'-dideoxyadenosine (F-ddA) is an acid-stable purine dideoxynucleoside analog active against a wide spectrum of human immunodeficiency virus type 1 (HIV-1) and HIV-2 strains in...

In vitro inhibition of the infectivity and replication of human immunodeficiency virus type 1 by combination of antiretroviral 2',3'-dideoxynucleosides and virus-binding inhibitors.

Hayashi, S, Fine, R L, Chou, T C, Currens, M J, Broder, S, Mitsuya, H

We tested the in vitro inhibitory activities of three 2',3'-dideoxynucleosides and two inhibitors of viral binding in combinations against the infectivity and cytopathic effect of human...

Cyclobut-A and cyclobut-G, carbocyclic oxetanocin analogs that inhibit the replication of human immunodeficiency virus in T cells and monocytes and macrophages in vitro.

Hayashi, S, Norbeck, D W, Rosenbrook, W, Fine, R L, Matsukura, M, Plattner, J J, ...

Two newly synthesized carbocyclic oxetanocin analogs, (+/-)-9-[(1 beta,2 alpha,3 beta)-2,3-bis(hydroxymethyl)-1-cyclobutyl]adenine (cyclobut-A) and (+/-)-9-[(1 beta,2 alpha,3...

Protein binding of human immunodeficiency virus protease inhibitor KNI-272 and alteration of its in vitro antiretroviral activity in the presence of high concentrations of proteins.

Kageyama, S, Anderson, B D, Hoesterey, B L, Hayashi, H, Kiso, Y, Flora, K P, ...

KNI-272 represents a peptide-based protease inhibitor having potent antiretroviral activity against human immunodeficiency virus (HIV) in vitro. The structure contains allophenylnorstatine...

In vitro inhibition of human immunodeficiency virus (HIV) type 1 replication by C2 symmetry-based HIV protease inhibitors as single agents or in combinations.

Kageyama, S, Weinstein, J N, Shirasaka, T, Kempf, D J, Norbeck, D W, Plattner, J J, ...

C2 symmetry-based human immunodeficiency virus (HIV) protease inhibitors were examined in vitro as single agents or in combination with 3'-azido-2',3'-dideoxythymidine (AZT) or 2',3'-dideoxyinosine...

Human immunodeficiency virus type 1 infection of H9 cells induces increased glucose transporter expression.

Sorbara, L R, Maldarelli, F, Chamoun, G, Schilling, B, Chokekijcahi, S, Staudt, L, ...

A clone obtained from a differential display screen for cellular genes with altered expression during human immunodeficiency virus (HIV) infection matched the sequence for the human GLUT3...

Central nervous system targeting of 2',3'-dideoxyinosine via adenosine deaminase-activated 6-halo-dideoxypurine prodrugs.

Morgan, M E, Chi, S C, Murakami, K, Mitsuya, H, Anderson, B D

AIDS dementia complex is a neurologic disorder, characterized by increasingly severe cognitive, behavioral, and motor impairment, which is associated with human immunodeficiency virus (HIV) infection...

Adenallene and cytallene: acyclic-nucleoside analogues that inhibit replication and cytopathic effect of human immunodeficiency virus in vitro.

Hayashi, S, Phadtare, S, Zemlicka, J, Matsukura, M, Mitsuya, H, Broder, S

Although several antiretroviral compounds are already known, almost no acyclic nucleoside derivatives lacking an oxacyclopentane have been reported to exert significant inhibition against human...

Regulation of viral expression of human immunodeficiency virus in vitro by an antisense phosphorothioate oligodeoxynucleotide against rev (art/trs) in chronically infected cells.

Matsukura, M, Zon, G, Shinozuka, K, Robert-Guroff, M, Shimada, T, Stein, C A, ...

In this report, we demonstrate the sequence-specific suppression of viral expression in T cells chronically infected with human immunodeficiency virus 1 (HIV-1), using antisense phosphorothioate...

Differential phosphorylation of azidothymidine, dideoxycytidine, and dideoxyinosine in resting and activated peripheral blood mononuclear cells.

Gao, W Y, Shirasaka, T, Johns, D G, Broder, S, Mitsuya, H

The antiviral activity of azidothymidine (AZT), dideoxycytidine (ddC), and dideoxyinosine (ddI) against HIV-1 was comparatively evaluated in PHA-stimulated PBM. The mean drug concentration which...

Targeted and highly efficient gene transfer into CD4+ cells by a recombinant human immunodeficiency virus retroviral vector.

Shimada, T, Fujii, H, Mitsuya, H, Nienhuis, A W

We have established a recombinant HIV gene transfer system based on transient expression of the HIV packaging functions and a recombinant vector genome in monkey kidney Cos cells. The recombinant HIV...

Phosphorothioate analogs of oligodeoxynucleotides: inhibitors of replication and cytopathic effects of human immunodeficiency virus.

Matsukura, M, Shinozuka, K, Zon, G, Mitsuya, H, Reitz, M, Cohen, J S, ...

Nuclease-resistant phosphorothioate analogs of certain oligodeoxynucleotides have been tested in vitro as antiviral agents against human immunodeficiency virus (HIV) in human T cells....

Long-term inhibition of human T-lymphotropic virus type III/lymphadenopathy-associated virus (human immunodeficiency virus) DNA synthesis and RNA expression in T cells protected by 2',3'-dideoxynucleosides in vitro.

Mitsuya, H, Jarrett, R F, Matsukura, M, Di Marzo Veronese, F, DeVico, A L, Sarngadharan, M G, ...

We report that 2',3'-dideoxyadenosine and 2',3'-dideoxycytidine inhibit retroviral DNA synthesis and mRNA expression in T cells exposed to the virus that causes acquired immunodeficiency syndrome,...

Broad spectrum antiretroviral activity of 2',3'-dideoxynucleosides.

Dahlberg, J E, Mitsuya, H, Blam, S B, Broder, S, Aaronson, S A

Certain dideoxynucleosides have been shown to markedly inhibit the infectivity of human T-lymphotropic virus type III/lymphadenopathy-associated virus, the causative agent of acquired...

Inhibition of the in vitro infectivity and cytopathic effect of human T-lymphotrophic virus type III/lymphadenopathy-associated virus (HTLV-III/LAV) by 2',3'-dideoxynucleosides.

Mitsuya, H, Broder, S

Human T-lymphotropic virus type III (HTLV-III)/lymphadenopathy-associated virus (LAV) is a a newly discovered lymphotropic retrovirus that is cytopathic for helper/inducer T cells in vitro. This...

Human monoclonal antibody directed against an envelope glycoprotein of human T-cell leukemia virus type I.

Matsushita, S, Robert-Guroff, M, Trepel, J, Cossman, J, Mitsuya, H, Broder, S

We report the production and characterization of a human monoclonal antibody reactive against the major envelope glycoprotein of human T-cell leukemia virus type I (HTLV-I), a virus linked to the...

Correction of adenosine deaminase deficiency in cultured human T and B cells by retrovirus-mediated gene transfer.

Kantoff, P W, Kohn, D B, Mitsuya, H, Armentano, D, Sieberg, M, Zwiebel, J A, ...

A retroviral vector called SAX, containing the cloned human cDNA for adenosine deaminase (ADA), has been constructed and used to introduce the ADA gene into cultured T- and B-lymphocyte lines derived...

Phosphorylation of 3'-azido-3'-deoxythymidine and selective interaction of the 5'-triphosphate with human immunodeficiency virus reverse transcriptase.

Furman, P A, Fyfe, J A, St Clair, M H, Weinhold, K, Rideout, J L, Freeman, G A, ...

The thymidine analog 3'-azido-3'-deoxythymidine (BW A509U, azidothymidine) can inhibit human immunodeficiency virus (HIV) replication effectively in the 50-500 nM range [Mitsuya, H., Weinhold, K. J.,...

3'-Azido-3'-deoxythymidine (BW A509U): an antiviral agent that inhibits the infectivity and cytopathic effect of human T-lymphotropic virus type III/lymphadenopathy-associated virus in vitro.

Mitsuya, H, Weinhold, K J, Furman, P A, St Clair, M H, Lehrman, S N, Gallo, R C, ...

The acquired immune deficiency syndrome (AIDS) is thought to result from infection of T cells by a pathogenic human retrovirus, human T-lymphotropic virus type III (HTLV-III) or...

Infection of human T lymphotropic virus-I-specific immune T cell clones by human T lymphotropic virus-I.

Mitsuya, H, Jarrett, R F, Cossman, J, Cohen, O J, Kao, C S, Guo, H G, ...

Human T lymphotropic virus-I (HTLV-I)-specific T cell lines were established and cloned. K5, an OKT8+ clone bearing multiple proviral integration sites, retained its HTLV-I-specific cytotoxicity and...

Alterations in cytotoxic and helper T cell function after infection of T cell clones with human T cell leukemia virus, type I.

Yarchoan, R, Guo, H G, Reitz, M, Maluish, A, Mitsuya, H, Broder, S

HTLV-I is a transforming human retrovirus that is an etiologic agent of adult T cell leukemia/lymphoma. To investigate the effects of this virus on T cell functions, two OKT3+, OKT4+, OKT8- cytotoxic...

Pharmacological inhibition of in vitro infectivity of human T lymphotropic virus type I.

Matsushita, S, Mitsuya, H, Reitz, M S, Broder, S

Human T lymphotropic virus type I (HTLV-I) is an exogenous RNA tumor virus etiologically linked to adult T cell leukemia and related diseases. In this paper, we describe that two...