Scientific Commons: H. N. Arst

Mutational analysis reveals dispensability of the N-terminal region of the Aspergillus transcription factor mediating nitrogen metabolite repression. (1995)

Langdon, T., Sheerins, A., Ravagnani, A., Gielkens, M., Caddick, M.X., Arst, H.N.

Extracellular arabinases in Aspergillus nidulans: the effect of different cre mutations on enzyme levels. (1994)

Arst, H.N.Jr., Flipphi, M.J.A., Visser, J.

A translocation activating the cryptic nitrogen regulation gene areB inactivates a previously unidentified gene involved in glycerol utilisation in Aspergillus nidulans. (1990)

Arst, H.N., Hondmann, D.H.A., Visser, J.

Characterization of the pH signal transduction pathway gene palA of Aspergillus nidulans and identification of possible homologs.

Negrete-Urtasun, S, Denison, S H, Arst, H N

We have cloned the palA gene of Aspergillus nidulans, one of six genes participating in ambient pH signal transduction in a regulatory circuit mediating pH regulation of gene expression. The derived...

Virulence studies of Aspergillus nidulans mutants requiring lysine or p-aminobenzoic acid in invasive pulmonary aspergillosis.

Tang, C M, Smith, J M, Arst, H N, Holden, D W

To identify steps in fungal intermediary metabolism required by Aspergillus spp. during invasive pulmonary aspergillosis, we have developed murine models involving Aspergillus nidulans as the...

Quantitation of oligohistidine fusion proteins using 63Ni2+ chelation.

Ravagnani, A, Arst, H N

Methylammonium Resistance in Aspergillus nidulans

Arst, H. N., Cove, D. J.

Mutants of Aspergillus nidulans resistant to methylammonium toxicity are simultaneously derepressed in the presence of ammonium for apparently all ammonium-repressible activities. Enzyme assays...

The Aspergillus PacC zinc finger transcription factor mediates regulation of both acid- and alkaline-expressed genes by ambient pH.

Tilburn, J, Sarkar, S, Widdick, D A, Espeso, E A, Orejas, M, Mungroo, J, ...

The pH regulation of gene expression in Aspergillus nidulans is mediated by pacC, whose 678 residue-derived protein contains three putative Cys2His2 zinc fingers. Ten pacCc mutations mimicking growth...

pH regulation is a major determinant in expression of a fungal penicillin biosynthetic gene.

Espeso, E A, Tilburn, J, Arst, H N, Peñalva, M A

Transcription of the ipnA gene encoding isopenicillin N synthetase, an enzyme of secondary metabolism, is under the control of the pH regulatory system in the fungus Aspergillus nidulans. External...

Nitrogen metabolite signalling involves the C-terminus and the GATA domain of the Aspergillus transcription factor AREA and the 3' untranslated region of its mRNA.

Platt, A, Langdon, T, Arst, H N, Kirk, D, Tollervey, D, Sanchez, J M, ...

AREA is a GATA transcription factor which mediates nitrogen metabolite repression in Aspergillus nidulans in response to intracellular glutamine levels. We have identified and localized three...

Cloning of the regulatory gene areA mediating nitrogen metabolite repression in Aspergillus nidulans.

Caddick, M X, Arst, H N, Taylor, L H, Johnson, R I, Brownlee, A G

The areA gene, which mediates nitrogen metabolite repression in the fungus Aspergillus nidulans, lies sufficiently close to a telomere that no indispensable gene can be distal to it. We were able...

Subtle hydrophobic interactions between the seventh residue of the zinc finger loop and the first base of an HGATAR sequence determine promoter-specific recognition by the Aspergillus nidulans GATA factor AreA.

Ravagnani, A, Gorfinkiel, L, Langdon, T, Diallinas, G, Adjadj, E, Demais, S, ...

A change of a universally conserved leucine to valine in the DNA-binding domain of the GATA factor AreA results in inability to activate some AreA-dependent promoters, including that of the uapA gene...

Control of nucleic acid synthesis in Aspergillus nidulans.

Arst, H N, Scazzocchio, C

Nitrate uptake in Aspergillus nidulans and involvement of the third gene of the nitrate assimilation gene cluster.

Brownlee, A G, Arst, H N

In Aspergillus nidulans, chlorate strongly inhibited net nitrate uptake, a process separate and distinct from, but dependent upon, the nitrate reductase reaction. Uptake was inhibited by uncouplers,...

The regulatory gene areA mediating nitrogen metabolite repression in Aspergillus nidulans. Mutations affecting specificity of gene activation alter a loop residue of a putative zinc finger.

Kudla, B, Caddick, M X, Langdon, T, Martinez-Rossi, N M, Bennett, C F, Sibley, S, ...

The regulatory gene areA mediating nitrogen metabolite repression in Aspergillus nidulans has been sequenced and its transcript mapped and orientated. A single ORF can encode a protein of 719 amino...