Nagase, H, Visse, R, Kashiwagi, M, Gendron, C, Itoh, Y
No abstract available.
Jozic, D., Bourenkov, G., Lim, N. H., Visse, R., Nagase, H., Bode, W., ...
Jozic, D., Bourenkov, G., Lim, N. H., Visse, R., Nagase, H., Bode, W., ...
TIMP-3 inhibits aggrecan breakdown in pig articular cartilage stimulated with IL-1 (2003)
Nagase, H, Gendron, C, Kashiwagi, M
No abstract available.
Germ-line mutations of the APC gene in 53 familial adenomatous polyposis patients.
Miyoshi, Y, Ando, H, Nagase, H, Nishisho, I, Horii, A, Miki, Y, ...
We searched for germ-line mutations of the APC gene in 79 unrelated patients with familial adenomatous polyposis using a ribonuclease protection analysis coupled with polymerase chain reaction...
Gruber, B L, Marchese, M J, Suzuki, K, Schwartz, L B, Okada, Y, Nagase, H, ...
Mast cells have been implicated in the pathogenesis of the matrix degradation observed in the cartilaginous and osseous structures of the rheumatoid joint. We previously reported that human mast cell...
Morodomi, T, Ogata, Y, Sasaguri, Y, Morimatsu, M, Nagase, H
The precursor of matrix metalloproteinase 9 (proMMP-9), also known as '92 kDa progelatinase/type IV procollagenase', was purified from the conditioned medium of U937 monocytic leukaemia and HT1080...
Okada, Y, Harris, E D, Nagase, H
Two active forms (Mr 45,000 and 28,000) of a metalloendopeptidase that digest proteoglycans and other extracellular matrix components of connective tissues have previously been purified from...
Degradation of basement membranes by human matrix metalloproteinase 3 (stromelysin).
Bejarano, P A, Noelken, M E, Suzuki, K, Hudson, B G, Nagase, H
Connective tissue cells synthesize and secrete a group of matrix metalloproteinases (MMPs), all of which are capable of degrading the extracellular-matrix components. One of them, MMP-3 (stromelysin)...
Suzuki, K, Lees, M, Newlands, G F, Nagase, H, Woolley, D E
Histological studies have previously demonstrated an association between mast-cell activation/degranulation and areas of connective-tissue lysis in vivo; in addition, mast-cell extracts have been...
Tissue inhibitor of metalloproteinases (TIMP)-2 forms a noncovalent complex with the precursor of matrix metalloproteinase 2 (proMMP-2, progelatinase A) through interaction of the C-terminal domain...
Gunja-Smith, Z, Nagase, H, Woessner, J F
The 'neutral' proteoglycan-degrading metalloproteinase of human articular cartilage was purified 3,500-fold by use of an anti-(matrix metalloproteinase-3) immunoglobulin G affinity column. Molecular...
Proactivator-dependent activation of procollagenase induced by treatment with EGTA.
Vater, C A, Nagase, H, Harris, E D
A new mechanism for activation of the proactivator of procollagenase [Vater, Nagase & Harris (1983) J. Biol. Chem. 258, 9374-9382] has been found. Collagenolytic and other proteolytic enzyme...
Sato, T, Ito, A, Mori, Y, Yamashita, K, Hayakawa, T, Nagase, H
Rabbit uterine cervical fibroblasts produced a large amount of matrix metalloproteinases (MMPs) such as collagenase (MMP-1) and stromelysin (MMP-3) and a small relatively amount of tissue inhibitor...
Nagase, H, Brinckerhoff, C E, Vater, C A, Harris, E D
Monolayer cultures of rabbit synovial fibroblasts stimulated with phorbol myristate acetate to produce large amounts of collagenase (EC 3.4.24.7) were used to study the biosynthesis and secretion of...
Human plasma kallikrein. A rapid purification method with high yield.
A simple method for isolation of kallikrein from human plasma is described. Before activation of the enzyme with acetone, the plasma was treated with 0.2 M-methylamine at pH 8.2 to inactivate alpha...
Nagase, H, Suzuki, K, Cawston, T E, Brew, K
Tissue inhibitors of metalloproteinases (TIMPs) inhibit matrix metalloproteinases (MMPs) by forming a 1:1 stoichiometric complex, but the inhibition mechanism of these inhibitors is not known. Here...
Germ-line mutations of the APC gene in 53 familial adenomatous polyposis patients.
Miyoshi, Y, Ando, H, Nagase, H, Nishisho, I, Horii, A, Miki, Y, ...
We searched for germ-line mutations of the APC gene in 79 unrelated patients with familial adenomatous polyposis using a ribonuclease protection analysis coupled with polymerase chain reaction...
Gruber, B L, Marchese, M J, Suzuki, K, Schwartz, L B, Okada, Y, Nagase, H, ...
Mast cells have been implicated in the pathogenesis of the matrix degradation observed in the cartilaginous and osseous structures of the rheumatoid joint. We previously reported that human mast cell...
Morodomi, T, Ogata, Y, Sasaguri, Y, Morimatsu, M, Nagase, H
The precursor of matrix metalloproteinase 9 (proMMP-9), also known as '92 kDa progelatinase/type IV procollagenase', was purified from the conditioned medium of U937 monocytic leukaemia and HT1080...
Okada, Y, Harris, E D, Nagase, H
Two active forms (Mr 45,000 and 28,000) of a metalloendopeptidase that digest proteoglycans and other extracellular matrix components of connective tissues have previously been purified from...
Degradation of basement membranes by human matrix metalloproteinase 3 (stromelysin).
Bejarano, P A, Noelken, M E, Suzuki, K, Hudson, B G, Nagase, H
Connective tissue cells synthesize and secrete a group of matrix metalloproteinases (MMPs), all of which are capable of degrading the extracellular-matrix components. One of them, MMP-3 (stromelysin)...
Suzuki, K, Lees, M, Newlands, G F, Nagase, H, Woolley, D E
Histological studies have previously demonstrated an association between mast-cell activation/degranulation and areas of connective-tissue lysis in vivo; in addition, mast-cell extracts have been...
Tissue inhibitor of metalloproteinases (TIMP)-2 forms a noncovalent complex with the precursor of matrix metalloproteinase 2 (proMMP-2, progelatinase A) through interaction of the C-terminal domain...
Gunja-Smith, Z, Nagase, H, Woessner, J F
The 'neutral' proteoglycan-degrading metalloproteinase of human articular cartilage was purified 3,500-fold by use of an anti-(matrix metalloproteinase-3) immunoglobulin G affinity column. Molecular...
Proactivator-dependent activation of procollagenase induced by treatment with EGTA.
Vater, C A, Nagase, H, Harris, E D
A new mechanism for activation of the proactivator of procollagenase [Vater, Nagase & Harris (1983) J. Biol. Chem. 258, 9374-9382] has been found. Collagenolytic and other proteolytic enzyme...
Sato, T, Ito, A, Mori, Y, Yamashita, K, Hayakawa, T, Nagase, H
Rabbit uterine cervical fibroblasts produced a large amount of matrix metalloproteinases (MMPs) such as collagenase (MMP-1) and stromelysin (MMP-3) and a small relatively amount of tissue inhibitor...
Nagase, H, Brinckerhoff, C E, Vater, C A, Harris, E D
Monolayer cultures of rabbit synovial fibroblasts stimulated with phorbol myristate acetate to produce large amounts of collagenase (EC 3.4.24.7) were used to study the biosynthesis and secretion of...
Human plasma kallikrein. A rapid purification method with high yield.
A simple method for isolation of kallikrein from human plasma is described. Before activation of the enzyme with acetone, the plasma was treated with 0.2 M-methylamine at pH 8.2 to inactivate alpha...
Nagase, H, Suzuki, K, Cawston, T E, Brew, K
Tissue inhibitors of metalloproteinases (TIMPs) inhibit matrix metalloproteinases (MMPs) by forming a 1:1 stoichiometric complex, but the inhibition mechanism of these inhibitors is not known. Here...
Okada, Y, Takeuchi, N, Tomita, K, Nakanishi, I, Nagase, H
Metalloproteinases produced by connective tissue cells may play a key part in the destruction of joints in rheumatoid arthritis. Matrix metalloproteinase 3 (MMP-3; stromelysin) capable of degrading...
Epithelial carcinogenesis in the mouse: correlating the genetics and the biology.
Frame, S, Crombie, R, Liddell, J, Stuart, D, Linardopoulos, S, Nagase, H, ...
Tumour formation relies on a complex combination of genetic and environmental factors. In particular, the contributions from inherited predisposition genes as well as carcinogens, for example from...
Sasaguri, Y., Komiya, S., Sugama, K., Suzuki, K., Inoue, A., Morimatsu, M., ...
Matrix metalloproteinases play a central role in the catabolism of extracellular matrix macromolecules. Here the authors report that giant cell tumor of bone (GCT) produces two matrix...
Zhang, Y, Yasuda, T, Wang, C R, Yoshimoto, T, Nagase, H, Takamoto, M, ...
The role of CD4 molecules in the autoimmune and lymphoproliferative syndrome caused by murine Fas mutations was studied using the novel systemic lupus erythematosus (SLE) model, MRL-Faslprcg/Faslprcg...
MATSUSHITA, N, KASHIWAGI, M, WAIT, R, NAGAYOSHI, R, NAKAMURA, M, MATSUDA, T, ...
The aim of the present study was to evaluate levels of soluble CD 163 in sera and fluids from rheumatoid arthritis (RA) patients and elucidate the mechanism that regulates the shedding of CD163....
Palytoxin-induced contraction and release of endogenous noradrenaline in rat tail artery.
Karaki, H., Nagase, H., Ohizumi, Y., Satake, N., Shibata, S.
1. The mechanism of the contractile effect of a potent marine toxin, palytoxin (PTX) on the rat isolated tail artery was examined. 2. PTX (10(-7) M) induced a contraction in the tail artery which was...
Karaki, H., Mitsui, M., Nagase, H., Ozaki, H., Shibata, S., Uemura, D.
1. Effects of okadaic acid, a toxin isolated from marine sponges, on smooth muscle contraction and platelet activation were examined. 2. Contractions in rabbit aorta induced by high concentrations of...
Karaki, H., Nagase, H., Ozaki, H., Urakawa, N., Weiss, G. B.
Effects of a Ca2+ channel facilitator, CGP 28,392, on smooth muscle contractions were examined in order to delineate characteristics of Ca2+ channels in rabbit and rat aortae and guinea-pig taenia...
Maher, E R, Barton, D E, Slatter, R, Koch, D J, Jones, M H, Nagase, H, ...
A population based clinical and molecular genetic study of familial adenomatous polyposis coli (FAPC) was performed to investigate the value of molecular genetic analysis and ophthalmological...
Effects of exposure to microwaves on cellular immunity and placental steroids in pregnant rats.
Nakamura, H, Seto, T, Nagase, H, Yoshida, M, Dan, S, Ogino, K
OBJECTIVES: Microwaves produce various detrimental changes based on actions of heat or non-specific stress, although the effects of microwaves on pregnant organisms has not been uniform. This study...
Wang, C-R, Hino, A, Yoshimoto, T, Nagase, H, Kato, T, Hirokawa, K, ...
Delayed-type hypersensitivity (DTH) is an important in vivo manifestation of cell-mediated immunity. We examined the DTH response to methylated bovine serum albumin of a novel mutant strain of mice...
Ito, A., Nakajima, S., Sasaguri, Y., Nagase, H., Mori, Y.
No measurable amounts of matrix metalloproteinases (MMPs) were produced by human breast adenocarcinoma cell lines MCF-7 and BT-20 in culture. When MCF-7 cells were co-cultured with human dermal...