Rodenberg, S., Steinberg, W., Piper, J., Nickerson, Kenneth, Vary, J., Epstein, R., ...
The rate of protein and ribonucleic acid (RNA) synthesis was examined during the outgrowth of spores of Bacillus cereus T in a chemically defined medium. RNA synthesis started 2.5 min after the...
THE EFFECT OF OXIDATIVE RANCIDITY IN UNSATURATED FATTY ACIDS ON THE GERMINATION OF BACTERIAL SPORES1
Wang, Y, Mahler, I, Levinson, H S, Halvorson, H O
A 7.9-kilobase (kb) chromosomal fragment was cloned from a mercury-resistant Bacillus sp. In Escherichia coli, in the presence of a second plasmid carrying functional transport genes, resistance to...
Boschwitz, H, Halvorson, H O, Keynan, A, Milner, Y
Trypsin-like enzymes were studied in dormant, activated, and germinated spores of Bacillus cereus T. Dormant spores contained two heat-labile enzyme activities. One was extractable with 2 M KCl and...
THE EFFECT OF POLY U SIZE ON THE INCORPORATION OF PHENYLALANINE IN THE CELL-FREE YEAST SYSTEM*
Marcus, L., Bretthauer, R. K., Bock, R. M., Halvorson, H. O.
Effect of inhibitors of trypsin-like proteolytic enzymes Bacillus cereus T spore germination.
Boschwitz, H, Milner, Y, Keynan, A, Halvorson, H O, Troll, W
The germination of Bacillus cereus T spore suspensions is partially prevented by several inhibitors of trypsin-like enzymes. Leupeptin, antipain, and tosyl-lysine-chloromethyl ketone are effective...
Ribosomal DNA magnification in Saccharomyces cerevisiae.
Strains monosomic for chromosome I of Saccharomyces cerevisiae contain 25 to 35% fewer rRNA genes than do normal diploid strains. When these strains are repeatedly subcultured, colonies are isolated...
Transformation of Escherichia coli and Bacillus subtilis with a hybrid plasmid molecule.
A hybrid molecule constructed from Escherichia coli plasmid pMB9 and a fragment of Bacillus subtilis 168 deoxyribonucleic acid functions in cells of leu-E. coli, converting them to leucine...
Sena, E P, Welch, J W, Halvorson, H O, Fogel, S
To study nuclear and mitochondrial deoxyribonucleic acid (DNA) synthesis during the cell cycle, a 15N-labeled log-phase population of Saccharomyces cervisiae was shifted to 14N medium. After one-half...
Proteinase activities of Saccharomyces cerevisiae during sporulation.
Sporulation in Saccharomyces cerevisiae occurs in the absence of a exogenous nitrogen source. Thus, the internal amino acid pool and the supply of nitrogen compounds from protein and nucleic acid...
Cadmium- and mercury-resistant Bacillus strains from a salt marsh and from Boston Harbor.
Mahler, I, Levinson, H S, Wang, Y, Halvorson, H O
Bacteria resistant to cadmium or mercury or both were isolated from the Great Sippewissett Marsh (Cape Cod, Mass.) and from Boston Harbor. Many of these metal-resistant isolates were gram-positive...
Nature of Ribonucleic Acid Synthesis During Early Sporulation in Saccharomyces cerevisiae
Chaffin, W. L., Sogin, S. J., Halvorson, H. O.
Phosphate uptake in sporulating cultures of Saccharomyces cerevisiae has been found to occur approximately 2 h after the transfer to sporulation medium. Early ribonucleic acid synthesis begins at...
Use of Yeast Populations Fractionated by Zonal Centrifugation to Study the Cell Cycle
Sebastian, J., Carter, B. L. A., Halvorson, H. O.
Zonal centrifugation in a sucrose density gradient was used to separate yeast cells primarily by size and thus by age in the cell cycle. This approach provides an alternative to synchronous growth...
Conditional Mutants of Meiosis in Yeast
Esposito, Michael S., Esposito, Rochelle E., Arnaud, Maryvonne, Halvorson, H. O.
Three temperature-sensitive mutants, spo1-1, spo2-1, and spo3-1, were characterized with respect to their behavior in sporulation medium at a restrictive temperature. The time of expression of the...
Appearance of a New Species of Ribonucleic Acid During Sporulation in Saccharomyces cerevisiae
Kadowaki, Kiyoshi, Halvorson, H. O.
In the course of study on ribonucleic acid (RNA) metabolism during sporulation in Saccharomyces cerevisiae, a new species of RNA (20S) was observed in sporulating cells by polyacrylamide gel...
Kadowaki, Kiyoshi, Halvorson, H. O.
A new species of ribonucleic acid (RNA) was detected in sporulating culture of Saccharomyces cerevisiae. This RNA was isolated by sucrose density gradient centrifugation and polyacrylamide gel...
Preparation and Storage of Single Spores of Saccharomyces cerevisiae
Rousseau, P., Halvorson, H. O.
A method is described for large-scale isolation and storage of single spores of Saccharomyces cerevisiae.
Rodenberg, S., Steinberg, W., Piper, J., Nickerson, K., Vary, J., Epstein, R., ...
The rate of protein and ribonucleic acid (RNA) synthesis was examined during the outgrowth of spores of Bacillus cereus T in a chemically defined medium. RNA synthesis started 2.5 min after the...
ACTIVATION OF BACTERIAL ENDOSPORES
Keynan, A., Evenchik, Z., Halvorson, H. O., Hastings, J. W.
A. Keynan (Israel Institute of Biological Research, Ness Ziona, Israel), Z. Evenchik, H. O. Halvorson, and J. W. Hastings. Studies on the activation of bacterial endospores. J. Bacteriol....
Kinetics of Germination of Bacillus Spores1
Vary, J. C. (University of Wisconsin, Madison), and H. O. Halvorson. Kinetics or germination of Bacillus spores. J. Bacteriol. 89:1340–1347. 1965.—The kinetics of germination of Bacillus cereus...
GERMINATION PROPERTIES OF SPORES WITH LOW DIPICOLINIC ACID CONTENT
Keynan, A., Murrell, W. G., Halvorson, H. O.
Keynan, A. (University of Wisconsin, Madison), W. G. Murrell, and H. O. Halvorson. Germination properties of spores with low dipicolinic acid content. J. Bacteriol. 83:395–399. 1962.—When the...
Okada, Hirosuke, Halvorson, H. O.
Okada, Hirosuke (University of Wisconsin, Madison), and H. O. Halvorson. Comparison of the active transport systems for α-thioethyl-d-glucopyranoside and maltose in Saccharomyces cerevisiae. J....
Sporulation of Yeast Harvested During Logarithmic Growth
Roth, Robert, Halvorson, H. O.
Rapid and abundant sporulation of yeast can be obtained, with cells harvested during logarithmic growth, by employing potassium acetate rather than glucose as a carbon source.
Characterization of Insoluble Protein Fractions of Mitochondria from Saccharomyces cerevisiae
Fiechter, A., Mian, F. A., Ris, H., Halvorson, H. O.
Saccharomyces cerevisiae was grown in a chemostat in the presence of excess oxygen. Cells harvested from fully derepressed and strongly repressed steady states show typical promitochondria-like...
Lipid Synthesis During Sporulation of Saccharomyces cerevisiae
Henry, Susan A., Halvorson, H. O.
Lipid synthesis was studied in both sporulating (diploid) and nonsporulating (haploid) cells of Saccharomyces cerevisiae. Two phases of lipid synthesis occur in diploid cells transferred to...
Arnaud, M, Mahler, I, Halvorson, H O, Boschwitz, H, Keynan, A
An Escherichia coli translation system supplemented with ribonucleic acid from sporulating Bacillus subtilis produces unique polypeptides which are missing among translation products of ribonucleic...
CALCIUM DIPICOLINIC ACID-INDUCED GERMINATION OF BACILLUS CEREUS SPORES
Keynan, A. (University of Wisconsin, Madison) and H. O. Halvorson. Calcium dipicolinic acid-induced germination of Bacillus cereus spores. J. Bacteriol. 83:100–105. 1962.—The germination of...
Initiation of Bacterial Spore Germination
To investigate the problem of initiation in bacterial spore germination, we isolated, from extracts of dormant spores of Bacillus cereus strain T and B. licheniformis, a protein that initiated spore...
Acetate Utilization and Macromolecular Synthesis During Sporulation of Yeast
Esposito, Michael S., Esposito, Rochelle E., Arnaud, Maryvonne, Halvorson, H. O.
Acetate utilization and macromolecule synthesis during sporulation (meiosis) of Saccharomyces cerevisiae were studied. When diploid cells are transferred from glucose nutrient medium to acetate...
Purification of a Salt-Requiring Enzyme from an Obligately Halophilic Bacterium
Holmes, P. K., Halvorson, H. O.
Holmes, P. K. (University of Illinois, Urbana), and H. O. Halvorson. Purification of a salt-requiring enzyme from an obligately halophilic bacterium. J. Bacteriol. 90: 312–315. 1965.—The...
Mutations affecting the signal sequence alter synthesis and secretion of yeast invertase.
Perlman, D, Raney, P, Halvorson, H O
Insertion mutations previously constructed within the proximal region of the yeast invertase signal sequence did not interfere with secretion or glycosylation of the enzyme. We now describe deletion...
Saunders, C A, Bostian, K A, Halvorson, H O
Thermal elution poly(U)-Sepharose chromatography was utilized to fractionate yeast mRNA based on poly(A) size. Analysis of the in vitro translation products of the fractionated RNAs in a wheat-embryo...
The MURFI linker for multiple reading frame insertion of a sense or nonsense codon into DNA.
Blunt-end palindromic DNA linkers with a central restriction site have been designed for the multiple reading frame insertion (abbreviated MURFI) of a sense or nonsense codon into DNA. We have...
Perlman, D, Halvorson, H O, Cannon, L E
Saccharomyces cerevisiae strain FH4C carries a single invertase structural gene, SUC2, but produces distinct invertase mRNAs and polypeptides for the secreted and cytoplasmic forms of the enzyme. The...
Bostian, K A, Lemire, J M, Cannon, L E, Halvorson, H O
Antibodies to repressible nonspecific acid phosphatase [APase; orthophosphoric-monoester phosphohydrolase (acid optimum), EC 3.1.3.2] purified from Saccharomyces cerevisiae were used to detect the in...
Genetic Evidence that Bacillus Bacteriophage φ105 Integrates by Insertion
Dean, D. H., Arnaud, M., Halvorson, H. O.
Genetic analysis by PBS1 transduction has demonstrated that bacterial markers spanning the φ105 attachment site are spread upon integration, accounting for insertion of one or more φ105 genomes.
New temperate bacteriophage for Bacillus subtilis, rho 11.
Dean, D H, Orrego, J C, Hutchison, K W, Halvorson, H O
A new temperate bacteriophage, rho11, isolated by J. Hoch, has been characterized. This new phage is very similar to the temperate phage phi3T in size (380 nm), host range, homoimmunity, DNA buoyant...
Regulation of repressible acid phosphatase gene transcription in Saccharomyces cerevisiae.
Lemire, J M, Willcocks, T, Halvorson, H O, Bostian, K A
We examined the genetic system responsible for transcriptional regulation of repressible acid phosphatase (APase; orthophosphoric-monoester phosphohydrolase [acid optimum, EC 3.1.3.2]) in...
Physiological control of repressible acid phosphatase gene transcripts in Saccharomyces cerevisiae.
Bostian, K A, Lemire, J M, Halvorson, H O
We have examined the regulation of repressible acid phosphatase (APase; orthophosphoric-monoester phosphohydrolase [acid optimum], EC 3.1.3.2) in Saccharomyces cerevisiae at the physiological and...
Perlman, D, Raney, P, Halvorson, H O
A single structural gene, SUC2, encodes both secreted and cytoplasmic invertase in Saccharomyces cerevisiae. It is known that the unprocessed polypeptides which differ by a secretion signal sequence...
Bostian, K A, Lemire, J M, Halvorson, H O
The synthesis of repressible acid phosphatase in Saccharomyces cerevisiae was examined under conditions of blocked derepression as described by Toh-e et al. (Mol. Gen. Genet. 162:139-149, 1978)....
Characterization of Mitochondrial Ribosomes from Yeast
Morimoto, H., Halvorson, H. O.
A method for the preparation of mitochondrial ribosomes from yeast is presented. Mitochondrial ribosomes differ from their cytoplasmic counterpart in nucleotide composition, electrophoretic mobility...
Magnification of genes coding for ribosomal RNA in Saccharomyces cerevisiae.
When a strain of Saccharomyces cerevisiae monosomic for chromosome I and initially deficient for 25% of the genes coding for ribosomal RNA is repeatedly subcultured, the number of these genes...
Specific inhibition of outgrowth of Bacillus subtilis spores by novobiocin.
Gottfried, M, Orrego, C, Keynan, A, Halvorson, H O
Spores of a Bacillus subtilis mutant temperature sensitive in deoxyribonucleic acid (DNA) replication proceeded through outgrowth at the nonpermissive temperature to the same extent as the wild-type...
THE EFFECT OF OXIDATIVE RANCIDITY IN UNSATURATED FATTY ACIDS ON THE GERMINATION OF BACTERIAL SPORES1
Wang, Y, Mahler, I, Levinson, H S, Halvorson, H O
A 7.9-kilobase (kb) chromosomal fragment was cloned from a mercury-resistant Bacillus sp. In Escherichia coli, in the presence of a second plasmid carrying functional transport genes, resistance to...
Boschwitz, H, Halvorson, H O, Keynan, A, Milner, Y
Trypsin-like enzymes were studied in dormant, activated, and germinated spores of Bacillus cereus T. Dormant spores contained two heat-labile enzyme activities. One was extractable with 2 M KCl and...
Specific inhibition of outgrowth of Bacillus subtilis spores by novobiocin.
Gottfried, M, Orrego, C, Keynan, A, Halvorson, H O
Spores of a Bacillus subtilis mutant temperature sensitive in deoxyribonucleic acid (DNA) replication proceeded through outgrowth at the nonpermissive temperature to the same extent as the wild-type...
THE EFFECT OF POLY U SIZE ON THE INCORPORATION OF PHENYLALANINE IN THE CELL-FREE YEAST SYSTEM*
Marcus, L., Bretthauer, R. K., Bock, R. M., Halvorson, H. O.
Effect of inhibitors of trypsin-like proteolytic enzymes Bacillus cereus T spore germination.
Boschwitz, H, Milner, Y, Keynan, A, Halvorson, H O, Troll, W
The germination of Bacillus cereus T spore suspensions is partially prevented by several inhibitors of trypsin-like enzymes. Leupeptin, antipain, and tosyl-lysine-chloromethyl ketone are effective...
Ribosomal DNA magnification in Saccharomyces cerevisiae.
Strains monosomic for chromosome I of Saccharomyces cerevisiae contain 25 to 35% fewer rRNA genes than do normal diploid strains. When these strains are repeatedly subcultured, colonies are isolated...
Transformation of Escherichia coli and Bacillus subtilis with a hybrid plasmid molecule.
A hybrid molecule constructed from Escherichia coli plasmid pMB9 and a fragment of Bacillus subtilis 168 deoxyribonucleic acid functions in cells of leu-E. coli, converting them to leucine...
Sena, E P, Welch, J W, Halvorson, H O, Fogel, S
To study nuclear and mitochondrial deoxyribonucleic acid (DNA) synthesis during the cell cycle, a 15N-labeled log-phase population of Saccharomyces cervisiae was shifted to 14N medium. After one-half...
Proteinase activities of Saccharomyces cerevisiae during sporulation.
Sporulation in Saccharomyces cerevisiae occurs in the absence of a exogenous nitrogen source. Thus, the internal amino acid pool and the supply of nitrogen compounds from protein and nucleic acid...
Cadmium- and mercury-resistant Bacillus strains from a salt marsh and from Boston Harbor.
Mahler, I, Levinson, H S, Wang, Y, Halvorson, H O
Bacteria resistant to cadmium or mercury or both were isolated from the Great Sippewissett Marsh (Cape Cod, Mass.) and from Boston Harbor. Many of these metal-resistant isolates were gram-positive...
Nature of Ribonucleic Acid Synthesis During Early Sporulation in Saccharomyces cerevisiae
Chaffin, W. L., Sogin, S. J., Halvorson, H. O.
Phosphate uptake in sporulating cultures of Saccharomyces cerevisiae has been found to occur approximately 2 h after the transfer to sporulation medium. Early ribonucleic acid synthesis begins at...
Use of Yeast Populations Fractionated by Zonal Centrifugation to Study the Cell Cycle
Sebastian, J., Carter, B. L. A., Halvorson, H. O.
Zonal centrifugation in a sucrose density gradient was used to separate yeast cells primarily by size and thus by age in the cell cycle. This approach provides an alternative to synchronous growth...
Conditional Mutants of Meiosis in Yeast
Esposito, Michael S., Esposito, Rochelle E., Arnaud, Maryvonne, Halvorson, H. O.
Three temperature-sensitive mutants, spo1-1, spo2-1, and spo3-1, were characterized with respect to their behavior in sporulation medium at a restrictive temperature. The time of expression of the...
Appearance of a New Species of Ribonucleic Acid During Sporulation in Saccharomyces cerevisiae
Kadowaki, Kiyoshi, Halvorson, H. O.
In the course of study on ribonucleic acid (RNA) metabolism during sporulation in Saccharomyces cerevisiae, a new species of RNA (20S) was observed in sporulating cells by polyacrylamide gel...
Kadowaki, Kiyoshi, Halvorson, H. O.
A new species of ribonucleic acid (RNA) was detected in sporulating culture of Saccharomyces cerevisiae. This RNA was isolated by sucrose density gradient centrifugation and polyacrylamide gel...
Preparation and Storage of Single Spores of Saccharomyces cerevisiae
Rousseau, P., Halvorson, H. O.
A method is described for large-scale isolation and storage of single spores of Saccharomyces cerevisiae.
Rodenberg, S., Steinberg, W., Piper, J., Nickerson, K., Vary, J., Epstein, R., ...
The rate of protein and ribonucleic acid (RNA) synthesis was examined during the outgrowth of spores of Bacillus cereus T in a chemically defined medium. RNA synthesis started 2.5 min after the...
ACTIVATION OF BACTERIAL ENDOSPORES
Keynan, A., Evenchik, Z., Halvorson, H. O., Hastings, J. W.
A. Keynan (Israel Institute of Biological Research, Ness Ziona, Israel), Z. Evenchik, H. O. Halvorson, and J. W. Hastings. Studies on the activation of bacterial endospores. J. Bacteriol....
Kinetics of Germination of Bacillus Spores1
Vary, J. C. (University of Wisconsin, Madison), and H. O. Halvorson. Kinetics or germination of Bacillus spores. J. Bacteriol. 89:1340–1347. 1965.—The kinetics of germination of Bacillus cereus...
GERMINATION PROPERTIES OF SPORES WITH LOW DIPICOLINIC ACID CONTENT
Keynan, A., Murrell, W. G., Halvorson, H. O.
Keynan, A. (University of Wisconsin, Madison), W. G. Murrell, and H. O. Halvorson. Germination properties of spores with low dipicolinic acid content. J. Bacteriol. 83:395–399. 1962.—When the...
Okada, Hirosuke, Halvorson, H. O.
Okada, Hirosuke (University of Wisconsin, Madison), and H. O. Halvorson. Comparison of the active transport systems for α-thioethyl-d-glucopyranoside and maltose in Saccharomyces cerevisiae. J....
Sporulation of Yeast Harvested During Logarithmic Growth
Roth, Robert, Halvorson, H. O.
Rapid and abundant sporulation of yeast can be obtained, with cells harvested during logarithmic growth, by employing potassium acetate rather than glucose as a carbon source.
Characterization of Insoluble Protein Fractions of Mitochondria from Saccharomyces cerevisiae
Fiechter, A., Mian, F. A., Ris, H., Halvorson, H. O.
Saccharomyces cerevisiae was grown in a chemostat in the presence of excess oxygen. Cells harvested from fully derepressed and strongly repressed steady states show typical promitochondria-like...
Lipid Synthesis During Sporulation of Saccharomyces cerevisiae
Henry, Susan A., Halvorson, H. O.
Lipid synthesis was studied in both sporulating (diploid) and nonsporulating (haploid) cells of Saccharomyces cerevisiae. Two phases of lipid synthesis occur in diploid cells transferred to...
Arnaud, M, Mahler, I, Halvorson, H O, Boschwitz, H, Keynan, A
An Escherichia coli translation system supplemented with ribonucleic acid from sporulating Bacillus subtilis produces unique polypeptides which are missing among translation products of ribonucleic...
CALCIUM DIPICOLINIC ACID-INDUCED GERMINATION OF BACILLUS CEREUS SPORES
Keynan, A. (University of Wisconsin, Madison) and H. O. Halvorson. Calcium dipicolinic acid-induced germination of Bacillus cereus spores. J. Bacteriol. 83:100–105. 1962.—The germination of...
Initiation of Bacterial Spore Germination
To investigate the problem of initiation in bacterial spore germination, we isolated, from extracts of dormant spores of Bacillus cereus strain T and B. licheniformis, a protein that initiated spore...
Acetate Utilization and Macromolecular Synthesis During Sporulation of Yeast
Esposito, Michael S., Esposito, Rochelle E., Arnaud, Maryvonne, Halvorson, H. O.
Acetate utilization and macromolecule synthesis during sporulation (meiosis) of Saccharomyces cerevisiae were studied. When diploid cells are transferred from glucose nutrient medium to acetate...
Purification of a Salt-Requiring Enzyme from an Obligately Halophilic Bacterium
Holmes, P. K., Halvorson, H. O.
Holmes, P. K. (University of Illinois, Urbana), and H. O. Halvorson. Purification of a salt-requiring enzyme from an obligately halophilic bacterium. J. Bacteriol. 90: 312–315. 1965.—The...
Mutations affecting the signal sequence alter synthesis and secretion of yeast invertase.
Perlman, D, Raney, P, Halvorson, H O
Insertion mutations previously constructed within the proximal region of the yeast invertase signal sequence did not interfere with secretion or glycosylation of the enzyme. We now describe deletion...
Saunders, C A, Bostian, K A, Halvorson, H O
Thermal elution poly(U)-Sepharose chromatography was utilized to fractionate yeast mRNA based on poly(A) size. Analysis of the in vitro translation products of the fractionated RNAs in a wheat-embryo...
The MURFI linker for multiple reading frame insertion of a sense or nonsense codon into DNA.
Blunt-end palindromic DNA linkers with a central restriction site have been designed for the multiple reading frame insertion (abbreviated MURFI) of a sense or nonsense codon into DNA. We have...
Perlman, D, Halvorson, H O, Cannon, L E
Saccharomyces cerevisiae strain FH4C carries a single invertase structural gene, SUC2, but produces distinct invertase mRNAs and polypeptides for the secreted and cytoplasmic forms of the enzyme. The...
Bostian, K A, Lemire, J M, Cannon, L E, Halvorson, H O
Antibodies to repressible nonspecific acid phosphatase [APase; orthophosphoric-monoester phosphohydrolase (acid optimum), EC 3.1.3.2] purified from Saccharomyces cerevisiae were used to detect the in...
Genetic Evidence that Bacillus Bacteriophage φ105 Integrates by Insertion
Dean, D. H., Arnaud, M., Halvorson, H. O.
Genetic analysis by PBS1 transduction has demonstrated that bacterial markers spanning the φ105 attachment site are spread upon integration, accounting for insertion of one or more φ105 genomes.
New temperate bacteriophage for Bacillus subtilis, rho 11.
Dean, D H, Orrego, J C, Hutchison, K W, Halvorson, H O
A new temperate bacteriophage, rho11, isolated by J. Hoch, has been characterized. This new phage is very similar to the temperate phage phi3T in size (380 nm), host range, homoimmunity, DNA buoyant...
Regulation of repressible acid phosphatase gene transcription in Saccharomyces cerevisiae.
Lemire, J M, Willcocks, T, Halvorson, H O, Bostian, K A
We examined the genetic system responsible for transcriptional regulation of repressible acid phosphatase (APase; orthophosphoric-monoester phosphohydrolase [acid optimum, EC 3.1.3.2]) in...
Physiological control of repressible acid phosphatase gene transcripts in Saccharomyces cerevisiae.
Bostian, K A, Lemire, J M, Halvorson, H O
We have examined the regulation of repressible acid phosphatase (APase; orthophosphoric-monoester phosphohydrolase [acid optimum], EC 3.1.3.2) in Saccharomyces cerevisiae at the physiological and...
Perlman, D, Raney, P, Halvorson, H O
A single structural gene, SUC2, encodes both secreted and cytoplasmic invertase in Saccharomyces cerevisiae. It is known that the unprocessed polypeptides which differ by a secretion signal sequence...
Bostian, K A, Lemire, J M, Halvorson, H O
The synthesis of repressible acid phosphatase in Saccharomyces cerevisiae was examined under conditions of blocked derepression as described by Toh-e et al. (Mol. Gen. Genet. 162:139-149, 1978)....
Characterization of Mitochondrial Ribosomes from Yeast
Morimoto, H., Halvorson, H. O.
A method for the preparation of mitochondrial ribosomes from yeast is presented. Mitochondrial ribosomes differ from their cytoplasmic counterpart in nucleotide composition, electrophoretic mobility...
Magnification of genes coding for ribosomal RNA in Saccharomyces cerevisiae.
When a strain of Saccharomyces cerevisiae monosomic for chromosome I and initially deficient for 25% of the genes coding for ribosomal RNA is repeatedly subcultured, the number of these genes...
OCCURRENCE, ISOLATION, AND CHARACTERIZATION OF POLYRIBOSOMES IN YEAST
Marcus, Leon, Ris, H., Halvorson, H. O., Bretthauer, R. K., Bock, R. M.
This report details the procedural requirements for preparing cell-free extracts of yeast rich in polyribosomes. This enabled us to demonstrate the occurrence of polyribosomes in yeast, to show their...
PERIODIC CHANGES IN RATE OF AMINO ACID UPTAKE DURING YEAST CELL CYCLE
Carter, B. L. A., Halvorson, H. O.
Uptake of amino acids is a complex process but in cells growing with ammonia as sole nitrogen source the initial uptake rate of amino acids is a measure of the transport capacity of the uptake system...