Morphological aspects of oligomeric protein structures (2005)
Ponstingl, H., Kabir, T., Gorse, D., Thornton, J.M.
Features of multimeric proteins are reviewed to shed light on the formation of protein assemblies from a structural perspective. The features comprise biochemical and geometric properties. They are...
Grunicke, Hans, Stoeck, Michael, Elsner, J., Seemann, G., Pfeiffer, C., Wegmann, B., ...
Peer Reviewed
Bischoff, F R, Krebber, H, Kempf, T, Hermes, I, Ponstingl, H
RanGAP1 is the GTPase activator for the nuclear Ras-related regulatory protein Ran, converting it to the putatively inactive GDP-bound state. Here, we report the amino acid sequence of RanGAP1,...
RanGAP1 induces GTPase activity of nuclear Ras-related Ran.
Bischoff, F R, Klebe, C, Kretschmer, J, Wittinghofer, A, Ponstingl, H
The nuclear Ras-related protein Ran binds guanine nucleotide and is involved in cell cycle regulation. Models of the signal pathway predict Ran to be active as Ran.GTP at the initiation of S phase...
Mitotic regulator protein RCC1 is complexed with a nuclear ras-related polypeptide.
We previously reported the purification of a complex of two proteins from human chromatin, consisting of a 47-kDa component called RCC1, which is a negative regulator of mitosis, and a 25-kDa...
Bischoff, F R, Maier, G, Tilz, G, Ponstingl, H
Several autoimmune sera from patients with Raynaud phenomenon decorated mammalian kinetochores and bound to a 47-kDa protein on immunoblots of nuclear lysates. Antibody affinity-purified from...
Marshallsay, C, Dickmanns, A, Bischoff, F R, Ponstingl, H, Fanning, E, Lührmann, R
GTP-hydrolysis, the small ras-related GTP-binding protein Ran and its cognate guanosine nucleotide exchange factor, the RCC1 gene product, have recently been identified as essential components of the...
Deane, R, Schäfer, W, Zimmermann, H P, Mueller, L, Görlich, D, Prehn, S, ...
We report the identification and characterization of a novel 124-kDa Ran binding protein, RanBP5. This protein is related to importin-beta, the key mediator of nuclear localization signal...
Complete amino acid sequence of alpha-tubulin from porcine brain.
Ponstingl, H, Krauhs, E, Little, M, Kempf, T
The amino acid sequence of alpha-tubulin from porcine brain was determined by automated and manual Edman degradation of eight sets of overlapping peptides. It comprises 450 residues plus a...
Complete amino acid sequence of beta-tubulin from porcine brain.
Krauhs, E, Little, M, Kempf, T, Hofer-Warbinek, R, Ade, W, Ponstingl, H
The primary structure of porcine brain beta-tubulin was determined by automated and manual Edman degradation of six sets of overlapping peptides. The protein consists of 445 amino acid residues and...
Common and distinct tubulin binding sites for microtubule-associated proteins.
Littauer, U Z, Giveon, D, Thierauf, M, Ginzburg, I, Ponstingl, H
A specific binding assay was developed that monitors the interaction of 125I-labeled microtubule-associated proteins (MAPs) with tubulin or its fragments bound to nitrocellulose membrane. To identify...
Co-activation of RanGTPase and inhibition of GTP dissociation by Ran-GTP binding protein RanBP1.
Bischoff, F R, Krebber, H, Smirnova, E, Dong, W, Ponstingl, H
RCC1 (the regulator of chromosome condensation) stimulates guanine nucleotide dissociation on the Ras-related nuclear protein Ran. Both polypeptides are components of a regulatory pathway that has...
Bossemeyer, D, Engh, R A, Kinzel, V, Ponstingl, H, Huber, R
The crystal structure of the porcine heart catalytic subunit of cAMP-dependent protein kinase in a ternary complex with the MgATP analogue MnAMP-PNP and a pseudosubstrate inhibitor peptide,...
Schröter, H, Maier, G, Ponstingl, H, Nordheim, A
Chicken erythrocyte nuclei were incubated with DNA intercalating agents in order to isolate from chromatin specific DNA-binding proteins whose binding specificity may be determined by DNA secondary...
Yrb4p, a yeast ran-GTP-binding protein involved in import of ribosomal protein L25 into the nucleus.
Schlenstedt, G, Smirnova, E, Deane, R, Solsbacher, J, Kutay, U, Görlich, D, ...
Gsp1p, the essential yeast Ran homologue, is a key regulator of transport across the nuclear pore complex (NPC). We report the identification of Yrb4p, a novel Gsp1p binding protein. The 123 kDa...
Bischoff, F R, Krebber, H, Kempf, T, Hermes, I, Ponstingl, H
RanGAP1 is the GTPase activator for the nuclear Ras-related regulatory protein Ran, converting it to the putatively inactive GDP-bound state. Here, we report the amino acid sequence of RanGAP1,...
RanGAP1 induces GTPase activity of nuclear Ras-related Ran.
Bischoff, F R, Klebe, C, Kretschmer, J, Wittinghofer, A, Ponstingl, H
The nuclear Ras-related protein Ran binds guanine nucleotide and is involved in cell cycle regulation. Models of the signal pathway predict Ran to be active as Ran.GTP at the initiation of S phase...
Mitotic regulator protein RCC1 is complexed with a nuclear ras-related polypeptide.
We previously reported the purification of a complex of two proteins from human chromatin, consisting of a 47-kDa component called RCC1, which is a negative regulator of mitosis, and a 25-kDa...
Bischoff, F R, Maier, G, Tilz, G, Ponstingl, H
Several autoimmune sera from patients with Raynaud phenomenon decorated mammalian kinetochores and bound to a 47-kDa protein on immunoblots of nuclear lysates. Antibody affinity-purified from...
Marshallsay, C, Dickmanns, A, Bischoff, F R, Ponstingl, H, Fanning, E, Lührmann, R
GTP-hydrolysis, the small ras-related GTP-binding protein Ran and its cognate guanosine nucleotide exchange factor, the RCC1 gene product, have recently been identified as essential components of the...
Deane, R, Schäfer, W, Zimmermann, H P, Mueller, L, Görlich, D, Prehn, S, ...
We report the identification and characterization of a novel 124-kDa Ran binding protein, RanBP5. This protein is related to importin-beta, the key mediator of nuclear localization signal...
Complete amino acid sequence of alpha-tubulin from porcine brain.
Ponstingl, H, Krauhs, E, Little, M, Kempf, T
The amino acid sequence of alpha-tubulin from porcine brain was determined by automated and manual Edman degradation of eight sets of overlapping peptides. It comprises 450 residues plus a...
Complete amino acid sequence of beta-tubulin from porcine brain.
Krauhs, E, Little, M, Kempf, T, Hofer-Warbinek, R, Ade, W, Ponstingl, H
The primary structure of porcine brain beta-tubulin was determined by automated and manual Edman degradation of six sets of overlapping peptides. The protein consists of 445 amino acid residues and...
Common and distinct tubulin binding sites for microtubule-associated proteins.
Littauer, U Z, Giveon, D, Thierauf, M, Ginzburg, I, Ponstingl, H
A specific binding assay was developed that monitors the interaction of 125I-labeled microtubule-associated proteins (MAPs) with tubulin or its fragments bound to nitrocellulose membrane. To identify...
Co-activation of RanGTPase and inhibition of GTP dissociation by Ran-GTP binding protein RanBP1.
Bischoff, F R, Krebber, H, Smirnova, E, Dong, W, Ponstingl, H
RCC1 (the regulator of chromosome condensation) stimulates guanine nucleotide dissociation on the Ras-related nuclear protein Ran. Both polypeptides are components of a regulatory pathway that has...
Bossemeyer, D, Engh, R A, Kinzel, V, Ponstingl, H, Huber, R
The crystal structure of the porcine heart catalytic subunit of cAMP-dependent protein kinase in a ternary complex with the MgATP analogue MnAMP-PNP and a pseudosubstrate inhibitor peptide,...
Schröter, H, Maier, G, Ponstingl, H, Nordheim, A
Chicken erythrocyte nuclei were incubated with DNA intercalating agents in order to isolate from chromatin specific DNA-binding proteins whose binding specificity may be determined by DNA secondary...
Yrb4p, a yeast ran-GTP-binding protein involved in import of ribosomal protein L25 into the nucleus.
Schlenstedt, G, Smirnova, E, Deane, R, Solsbacher, J, Kutay, U, Görlich, D, ...
Gsp1p, the essential yeast Ran homologue, is a key regulator of transport across the nuclear pore complex (NPC). We report the identification of Yrb4p, a novel Gsp1p binding protein. The 123 kDa...