Diacylglycerol kinase zeta inhibits G alpha q-induced atrial remodeling in transgenic mice (2009)
Hirose, M, Takeishi, Y, Niizeki, T, Shimojo, H, Nakada, T, Kubota, I, ...
Incomplete transformation of rat cells by a deletion mutant of adenovirus type 5.
Shiroki, K, Maruyama, K, Saito, I, Fukui, Y, Shimojo, H
Rat 3Y1 cells were infected with adenovirus type 5 (Ad5) wild type, dl312 (deletion of 902 base pairs between 1.5-4.5 map units), and dl313 (deletion of 2,350 base pairs between 3.5-10.5 map units)....
Ad5-HBL is a type 5 adenovirus bearing the large BglII fragment (2.8 kilobases; 87% of the total genome) of hepatitis B virus (HBV), subtype adr. Eight HBV RNAs expressed in HeLa cells infected with...
Saito, I, Oya, Y, Yamamoto, K, Yuasa, T, Shimojo, H
A novel helper-free adenovirus type 5 (Ad5) vector system, which utilizes a cloning site 0.2 kilobase (kb) from the right end of the genome, has been developed. To construct a nondefective Ad5...
Shiroki, K, Saito, I, Maruyama, K, Shimojo, H
A nondefective recombinant between adenovirus type 5 (Ad5) and type 12 (Ad12), rc-1 (Ad5 dl312, carrying the Ad12 E1A gene), was isolated from hamster cell foci transformed by a defective...
Fukui, Y, Saito, I, Shiroki, K, Shimojo, H
We isolated three adenovirus 12 early region 1B mutants (in205B, in205C, and dl205) by ligation of the cleaved DNA-protein complex and transfection of human embryo kidney cells with the ligation...
Characterization of a host range mutant of human adenovirus 12 defective in early region 1B.
Fukui, Y, Shiroki, K, Saito, I, Shimojo, H
We isolated an adenovirus 12 early region 1B mutant (in206B) by ligation of the cleaved DNA-protein complex and transfection of human embryo kidney cells with the ligation products. By deduction from...
Shiroki, K, Hashimoto, S, Saito, I, Fukui, Y, Fukui, Y, Kato, H, ...
Rat 3Y1 cells were transfected with recombinant gARC ( pSV2gpt carrying the adenovirus 12 early region 1 [E1] gene), and focus formation was observed in monolayer cultures after culture of cells in...
Expression of adenovirus type 12 E1A gene in monkey cells, using a simian virus 40 vector.
Oda, K, Kato, H, Saito, I, Sugano, S, Maruyama, K, Masuda, M, ...
Simian virus 40 (SV40) recombinants carrying the adenovirus type 12 E1A gene were constructed. The SV40 expression vector was constructed by removing most of the VP1 gene and an internal part of the...
Oda, K, Fukui, Y, Saito, I, Masuda, M, Shiroki, K, Shimojo, H
Simian virus 40 recombinant DNAs carrying the adenovirus type 12 E1B gene were constructed, propagated, and packaged in monkey cells. Monkey cells infected with the resulting virus stocks...
Shiroki, K, Saito, I, Maruyama, K, Fukui, Y, Imatani, Y, Oda, K I, ...
The adenovirus type 12 (Ad12) early region 1 (E1) gene was introduced into KB cells by using a dominant selection vector, pSV2-gpt, and over 80 Gpt+ KB cell clones were established. Three types of...
Small t protein of simian virus 40 is required for dense focus formation in a rat cell line.
Sugano, S, Yamaguchi, N, Shimojo, H
Incomplete foci were formed in a Fischer rat cell line infected with simian virus 40 detection mutants encoding defective small t antigen. The foci were small and rarely piled up. The fraction of...
Shiroki, K, Maruyama, K, Saito, I, Fukui, Y, Yazaki, K, Shimojo, H
Recombinants between an adenovirus type 5 (Ad5) deletion mutant and the Ad12 DNA fragment containing early region 1 (E1) were isolated from cells cotransfected with the EcoRI-C fragment of Ad12 DNA...
Kimura, T, Sawada, Y, Shinawawa, M, Shimizu, Y, Shiroki, K, Shimojo, H, ...
The nucleotide sequence of the entire transforming early region of E1b of the highly oncogenic adenovirus type 12 (Ad12) DNA has been determined. The total sequence (3860 base pairs) encompasses the...
Yoneyama, T, Hagiwara, A, Hara, M, Shimojo, H
A close relationship was demonstrated by oligonucleotide fingerprinting between genomes of the poliovirus type 2 Sabin vaccine strain and recent isolates from paralytic cases associated with...
Sawada, Y, Ojima, S, Shimojo, H, Shiroki, K, Fujinaga, K
Rat cell lines tranformed by viral DNA fragments, EcoRI-C and HindIII-G, of adenovirus type 12 DNA were analyzed for the viral transforming DNA sequences present in cell DNAs. Cell lines transformed...
Helper factor(s) for growth of adeno-associated virus in cells transformed by adenovirus 12.
Handa, H, Shiroki, K, Shimojo, H
Evidence is presented that a helper factor(s) for growth of adeno-associated virus (AAV) is present in cells transformed by adenovirus type 12 (Ad12). The growth of AAV was observed in heterokaryons...
Isolation of the viral DNA replication complex from adeno-associated virus type 1-infected cells.
The replication complex active in adeno-associated virus type 1 (AAV-1) DNA synthesis in vitro was solubilized, with a nonionic detergent, from the nuclei of human embryonic kidney cells coinfected...
Segawa, K, Oda, K, Yuasa, Y, Shiroki, K, Shimojo, H
Endogenous protein phosphorylation in chromatin and ribosomes of monkey, mouse, and rat cells transformed by DNA, RNA tumor viruses, and a chemical carcinogen revealed the association of a protein of...
Incomplete transformation of rat cells by a deletion mutant of adenovirus type 5.
Shiroki, K, Maruyama, K, Saito, I, Fukui, Y, Shimojo, H
Rat 3Y1 cells were infected with adenovirus type 5 (Ad5) wild type, dl312 (deletion of 902 base pairs between 1.5-4.5 map units), and dl313 (deletion of 2,350 base pairs between 3.5-10.5 map units)....
Ad5-HBL is a type 5 adenovirus bearing the large BglII fragment (2.8 kilobases; 87% of the total genome) of hepatitis B virus (HBV), subtype adr. Eight HBV RNAs expressed in HeLa cells infected with...
Saito, I, Oya, Y, Yamamoto, K, Yuasa, T, Shimojo, H
A novel helper-free adenovirus type 5 (Ad5) vector system, which utilizes a cloning site 0.2 kilobase (kb) from the right end of the genome, has been developed. To construct a nondefective Ad5...
Shiroki, K, Saito, I, Maruyama, K, Shimojo, H
A nondefective recombinant between adenovirus type 5 (Ad5) and type 12 (Ad12), rc-1 (Ad5 dl312, carrying the Ad12 E1A gene), was isolated from hamster cell foci transformed by a defective...
Fukui, Y, Saito, I, Shiroki, K, Shimojo, H
We isolated three adenovirus 12 early region 1B mutants (in205B, in205C, and dl205) by ligation of the cleaved DNA-protein complex and transfection of human embryo kidney cells with the ligation...
Characterization of a host range mutant of human adenovirus 12 defective in early region 1B.
Fukui, Y, Shiroki, K, Saito, I, Shimojo, H
We isolated an adenovirus 12 early region 1B mutant (in206B) by ligation of the cleaved DNA-protein complex and transfection of human embryo kidney cells with the ligation products. By deduction from...
Shiroki, K, Hashimoto, S, Saito, I, Fukui, Y, Fukui, Y, Kato, H, ...
Rat 3Y1 cells were transfected with recombinant gARC ( pSV2gpt carrying the adenovirus 12 early region 1 [E1] gene), and focus formation was observed in monolayer cultures after culture of cells in...
Expression of adenovirus type 12 E1A gene in monkey cells, using a simian virus 40 vector.
Oda, K, Kato, H, Saito, I, Sugano, S, Maruyama, K, Masuda, M, ...
Simian virus 40 (SV40) recombinants carrying the adenovirus type 12 E1A gene were constructed. The SV40 expression vector was constructed by removing most of the VP1 gene and an internal part of the...
Oda, K, Fukui, Y, Saito, I, Masuda, M, Shiroki, K, Shimojo, H
Simian virus 40 recombinant DNAs carrying the adenovirus type 12 E1B gene were constructed, propagated, and packaged in monkey cells. Monkey cells infected with the resulting virus stocks...
Shiroki, K, Saito, I, Maruyama, K, Fukui, Y, Imatani, Y, Oda, K I, ...
The adenovirus type 12 (Ad12) early region 1 (E1) gene was introduced into KB cells by using a dominant selection vector, pSV2-gpt, and over 80 Gpt+ KB cell clones were established. Three types of...
Small t protein of simian virus 40 is required for dense focus formation in a rat cell line.
Sugano, S, Yamaguchi, N, Shimojo, H
Incomplete foci were formed in a Fischer rat cell line infected with simian virus 40 detection mutants encoding defective small t antigen. The foci were small and rarely piled up. The fraction of...
Shiroki, K, Maruyama, K, Saito, I, Fukui, Y, Yazaki, K, Shimojo, H
Recombinants between an adenovirus type 5 (Ad5) deletion mutant and the Ad12 DNA fragment containing early region 1 (E1) were isolated from cells cotransfected with the EcoRI-C fragment of Ad12 DNA...
Kimura, T, Sawada, Y, Shinawawa, M, Shimizu, Y, Shiroki, K, Shimojo, H, ...
The nucleotide sequence of the entire transforming early region of E1b of the highly oncogenic adenovirus type 12 (Ad12) DNA has been determined. The total sequence (3860 base pairs) encompasses the...
Yoneyama, T, Hagiwara, A, Hara, M, Shimojo, H
A close relationship was demonstrated by oligonucleotide fingerprinting between genomes of the poliovirus type 2 Sabin vaccine strain and recent isolates from paralytic cases associated with...
Sawada, Y, Ojima, S, Shimojo, H, Shiroki, K, Fujinaga, K
Rat cell lines tranformed by viral DNA fragments, EcoRI-C and HindIII-G, of adenovirus type 12 DNA were analyzed for the viral transforming DNA sequences present in cell DNAs. Cell lines transformed...
Helper factor(s) for growth of adeno-associated virus in cells transformed by adenovirus 12.
Handa, H, Shiroki, K, Shimojo, H
Evidence is presented that a helper factor(s) for growth of adeno-associated virus (AAV) is present in cells transformed by adenovirus type 12 (Ad12). The growth of AAV was observed in heterokaryons...
Isolation of the viral DNA replication complex from adeno-associated virus type 1-infected cells.
The replication complex active in adeno-associated virus type 1 (AAV-1) DNA synthesis in vitro was solubilized, with a nonionic detergent, from the nuclei of human embryonic kidney cells coinfected...
Segawa, K, Oda, K, Yuasa, Y, Shiroki, K, Shimojo, H
Endogenous protein phosphorylation in chromatin and ribosomes of monkey, mouse, and rat cells transformed by DNA, RNA tumor viruses, and a chemical carcinogen revealed the association of a protein of...