Paragonimus heterotremus Infection in Nagaland: A New Focus of Paragonimiasis in India (2009)
Singh, T.S., Sugiyama, H., Umehara, A., Hiese, S., Khalo, K.
Purpose: To determine the prevalence of paragonimiasis among the patients who were attending the tuberculosis (TB) clinics at the Community Health Centre, Pfutsero, Phek District, Nagaland. To...
Chen, Jun-Yuan, Bottjer, David J., Davidson, Eric H., Li, Gang, Gao, Feng, Cameron, R. Andrew, ...
Microfossils from the Ediacaran Weng’an Phosphate Member of the Doushantuo Formation (Guizhou Province, southern China) have received widespread attention. The Doushantuo, which overlies the...
Petcov, S. T., Sugiyama, H., Takanishi, Y.
The connection between the neutrinoless double beta ((\beta\beta)_{0 \nu}-) decay effective Majorana mass, |M_{ee}|, and the branching ratios of the decays H^{\pm\pm}\to l^\pm {l'}^\pm, l,{l'}=...
Aoki, M., Akiyama, K., Fukuda, Y., Fukui, A., Funaki, Y., Furuta, H., ...
One of the current most-demanded experiments in neutrino physics is to measure the last mixing angle theta_13. KASKA is an experiment to detect new type of reactor neutrino oscillation and to measure...
Resolving theta(23) degeneracy by accelerator and reactor neutrino oscillation experiments (2006)
Hiraide, K, Minakata, H, Nakaya, T, Nunokawa, H, Sugiyama, H, Teves, WJC, ...
Systematic limits on sin22theta13 in neutrino oscillation experiments with multireactors (2006)
Sugiyama, H., Yasuda, O., Suekane, F., Horton-Smith, G. A.
Sensitivities to sin22theta13 without statistical errors ("systematic limit") are investigated in neutrino oscillation experiments with multiple reactors. Using an analytical approach, we show that...
Using an analytical approach, the sensitivity to sin^2{2theta_13} with infinite statistics in a spectral analysis is investigated in reactor neutrino oscillation experiments with one reactor and two...
Sugiyama, H., Yasuda, O., Suekane, F., Horton-Smith, G. A.
Sensitivities to sin^2{2theta_{13}} without statistical errors (``systematic limit'') are investigated in neutrino oscillation experiments with multiple reactors. Using an analytical approach, we...
White paper report on using nuclear reactors to search for a value of theta(13). (2004)
Anderson, K., Anjos, J.C., Ayres, D., Beacom, J., Bediaga, I., De Bellefon, A., ...
An International Working Group has been meeting to discuss ideas for a new Nuclear Reactor Neutrino Experiment at meetings in May 2003 (Alabama), October 2003 (Munich) and plans for March 2004...
White paper report on using nuclear reactors to search for a value of theta13 (2004)
Anderson, K., Anjos, J.C., Ayres, D., Beacom, J., Bediaga, I., De Bellefon, A., ...
White paper report on using nuclear reactors to search for a value of theta(13). (2004)
Anderson, K., Anjos, J.C., Ayres, D., Beacom, J., Bediaga, I., De Bellefon, A., ...
An International Working Group has been meeting to discuss ideas for a new Nuclear Reactor Neutrino Experiment at meetings in May 2003 (Alabama), October 2003 (Munich) and plans for March 2004...
White paper report on using nuclear reactors to search for a value of theta(13). (2004)
Anderson, K., Anjos, J.C., Ayres, D., Beacom, J., Bediaga, I., De Bellefon, A., ...
An International Working Group has been meeting to discuss ideas for a new Nuclear Reactor Neutrino Experiment at meetings in May 2003 (Alabama), October 2003 (Munich) and plans for March 2004...
White paper report on: Using nuclear reactors to search for a value of θ13 (2004)
Anderson,K., Anjos,J.C, Ayres,D., Beacom,J., Bediaga,I., De Bellefon,A., ...
White paper report on: Using nuclear reactors to search for a value of θ13 (2004)
Anderson, K., Anjos, J.C, Ayres, D., Beacom, J., Bediaga, I., De Bellefon, A., ...
Serological Screening Test for Any Botulinum Toxin Type (2003)
Methods are available to test samples directly for A, B or E botulinum toxin, If the toxin type is known. If the type is unknown, testing is complicated in that separate tests for the three types...
Reactor Measurement of $\theta_{13}$ and Its Complementarity to Long-Baseline Experiments (2002)
Minakata, H., Sugiyama, H., Yasuda, O., Inoue, K., Suekane, F.
A possibility to measure $\sin^22\theta_{13}$ using reactor neutrinos is examined in detail. It is shown that the sensitivity $\sin^22\theta_{13}>0.02$ can be reached with 20 ton-year data by placing...
Balboni, G, Pavani, M G, Spalluto, G, Tabrizi, M A, Clercq, E D, ...
We have synthesized and evaluated a series of hybrids, denoted 22--27, for in vitro cytotoxic activity against a variety of cancer cell lines. These hybrids represent a molecular combination of...
Amplification of pepleomycin-mediated DNA cleavage and apoptosis by unfused aromatic cations (2000)
Kawanishi, S, Oikawa, S, Kawanishi, M, Sugiyama, H, Saito, I, Strekowski, L, ...
Substrate recognition by Escherichia coli MutY using substrate analogs (1999)
Chepanoske, CL, Porello, SL, Fujiwara, T, Sugiyama, H, David, SS
Kataoka, T., Tsukamoto, O., Yunoki, E., Michihiro, K., Sugiyama, H., Shimizu, M., ...
Variation of 222Rn concentration in outdoor air, returns of a monostatic acoustic sounder and sensible heat flux are simultaneously observed at Okayama-shi in Japan. The sensible heat flux is...
Sugiyama, H, Kawai, K, Matsunaga, A, Fujimoto, K, Saito, I, Robinson, H, ...
Various oligonucleotides containing 8-methylguanine (m8G) have been synthesized and their structures and thermodynamic properties investigated. Introduction Of M8G into DNA sequences markedly...
Replication of DNA templates containing 5-formyluracil, a major oxidative lesion of thymine in DNA.
Zhang, Q M, Sugiyama, H, Miyabe, I, Matsuda, S, Saito, I, Yonei, S
5-Formyluracil (5-foU) is a major lesion of thymine produced in DNA by ionizing radiation and various chemical oxidants. To assess its biochemical effects on DNA replication, 22mer oligonucleotide...
Substrate recognition by Escherichia coli MutY using substrate analogs.
Chepanoske, C L, Porello, S L, Fujiwara, T, Sugiyama, H, David, S S
The Escherichia coli adenine glycosylase MutY is involved in the repair of 7,8-dihydro-8-oxo-2"-deoxyguanosine (OG):A and G:A mispairs in DNA. Our approach toward understanding recognition and...
Imidazole-imidazole pair as a minor groove recognition motif for T:G mismatched base pairs.
Yang, X L, Hubbard , R B, Lee, M, Tao, Z F, Sugiyama, H, Wang, A H
The T:G mismatched base pair is associated with many genetic mutations. Understanding its biological consequences may be aided by studying the structural perturbation of DNA caused by a T:G base pair...
Improved procedure for crystallization of Clostridium botulinum type A toxic complexes.
Sugiyama, H, Moberg, L J, Messer, S L
A modified protocol for the preparation of botulinum type A toxic crystals is decribed. The essential change was the substitution of chromatography on diethylaminoethyl-Sephadex for the step in which...
The genes for the Clostridium botulinum type G toxin complex are on a plasmid.
Zhou, Y, Sugiyama, H, Nakano, H, Johnson, E A
Clostridium botulinum type G produces a toxin complex that is composed of neurotoxin, hemagglutinin, and nontoxic nonhemagglutinin. The three genes encoding these proteins were closely linked on a...
SYMPOSIUM ON THE BIOLOGY OF BACTERIAL SPORES1
Williams, O. B., Lamanna, Carl, Knaysi, Georges, Wynne, E. Staten, Schmidt, C. F., Curran, Harold R., ...
Colony immunoblot assay of botulinal toxin.
Goodnough, M C, Hammer, B, Sugiyama, H, Johnson, E A
Botulinal neurotoxin in and around colonies of Clostridium botulinum types A, B, and E and of toxigenic Clostridium butyricum was detected by an enzyme-linked immunoassay procedure whereby the toxin...
Zhou, Y, Sugiyama, H, Johnson, E A
Two Clostridium butyricum strains from infant botulism cases produce a toxic molecule very similar to C. botulinum type E neurotoxin. Chromosomal, plasmid, and bacteriophage DNAs of toxigenic and...
Purification and Properties of Clostridium botulinum Type F Toxin
Clostridium botulinum type F toxin of proteolytic Langeland strain was purified. Toxin in whole cultures was precipitated with (NH4)2SO4. Extract of the precipitate was successively chromatographed...
Detection of Clostridium botulinum Toxin by Local Paralysis Elicited with Intramuscular Challenge
Sugiyama, H., Brenner, Shelley L., Dasgupta, B. R.
Clostridium botulinum toxin can be identified by a characteristic, acute local paralysis that follows its injection into the gastrocnemius (“calf” muscle) of mice The local botulism can be...
Simplified purification method for Clostridium botulinum type E toxin.
Clostridium botulinum type E toxin was purified in three chromatography steps. Toxin extracted from cells was concentrated by precipitation and dissolving in a small volume of citrate buffer. When...
Production of toxin by Clostridium botulinum type A strains cured by plasmids.
Weickert, M J, Chambliss, G H, Sugiyama, H
Twelve strains of Clostridium botulinum type A and seven strains of Clostridium sporogenes were screened for plasmids by agarose gel electrophoresis of cleared lysates of cells from 5 ml of...
Acute toxicity of aminoglycoside antibiotics as an aid in detecting botulism.
Wang, Y C, Burr, D H, Korthals, G J, Sugiyama, H
Gentamicin sulfate or neomycin sulfate injected intraperitoneally into 24- to 27-g mice at a dose of 6.2 mg per mouse elicited botulism-like responses in less than 30 min, but a dose of 3.1 mg per...
Affinity chromatography purification of type A botulinum neurotoxin from crystalline toxic complex.
Type A botulinum neurotoxin was purified from toxic crystals by adsorption to p-aminophenyl-beta-D-thiogalactopyranoside coupled to CH-Sepharose 4B. At pH 6.3, the toxic complex was held by the...
Toxicity in culture fluids of several Clostridium botulinum type A strains was assayed in mice and converted to weight equivalent. The toxin-related antigen in the samples was quantitated by a...
Nature of intracellular type A botulinum neurotoxin.
The neurotoxin in cells of young Clostridium botulinum type A culture was extracted with lysozyme. Highly purified neurotoxin preparation, obtained by processing the extract in two chromatographic...
Boticinogeny and Actions of the Bacteriocin1
Anastasio, Kathryn L., Soucheck, J. A., Sugiyama, H.
The bacteriocin, boticin E, was produced by only a few strains of those clostridia which are nontoxigenic but otherwise identical to Clostridium botulinum type E. Boticin preparations from four...
Brown, George W., King, Gretchen, Sugiyama, H.
When logarithmically growing cultures of Clostridium botulinum types A and E are treated with penicillin in a liquid medium containing 8% polyethylene glycol, protoplast-like spherical bodies are...
Fine Structure of Protoplasts and L-Forms of Clostridium botulinum Types A and E1
Brown, George W., Pate, J. L., Sugiyama, H.
Spherical bodies, that are obtained by adding penicillin and lysozyme to Clostridium botulinum types E and A cultures which are growing in an osmotically stabilized medium, are shown to be...
Bott, Thomas L., Johnson, Jodie, Foster, E. M., Sugiyama, H.
Bottom and shoreline sediments of Green Bay, northern Lake Michigan, and rivers of the Green Bay drainage basin, as well as soils of the surrounding land mass, were examined for Clostridium botulinum...
Rabbit Ileal Loop Response to Strains of Clostridium perfringens1
Duncan, Charles L., Sugiyama, H., Strong, Dorothy H.
The ligated loop of the rabbit intestine was investigated as a possible experimental model for the study of Clostridium perfringens food poisoning. The method of preparation of the challenge inoculum...
Comparison of toxins of Clostridium butyricum and Clostridium botulinum type E.
The toxin of Clostridium butyricum strains isolated from two infants with botulism is neutralized by antitoxin for type E botulinum toxin. This toxin and that of a C. botulinum type E strain were...
Sugiyama, H, Prather, J L, Woller, M J
Adult mice, made susceptible to Clostridium botulinum by feedings of metronidazole, were immobilized with an anesthetic and held for 30 min in isolators in which a fine powder of lyophilized pathogen...
Inactivation of botulinum and tetanus toxins by chelators.
Bhattacharyya, S D, Sugiyama, H
Purified type A botulinum toxin of about 10(6) mouse 50% lethal doses per ml was greater than 99.9% inactivated when incubated at pH 7.4 for 30 min at 37 degrees C in 20 mM 1,10-phenanthroline (PTL)...
Conventional adult mice were pretreated with metronidazole to make their intestinal tracts receptive to colonization by Clostridium botulinum. These mice, in groups of 10, were fed 0 (controls),...
Leukocytic Response in Monkeys Challenged with Staphylococcal Enterotoxin
Sugiyama, H. (University of Chicago, Chicago, Ill.), and E. M. McKissic, Jr. Leukocytic response in monkeys challenged with staphylococcal enterotoxin. J. Bacteriol. 92:349–352. 1966—The feeding...
Saito, I, Sugiyama, H, Matsuura, T, Ueda, K, Komano, T
A new procedure for T specific cleavage of DNA fragments utilizing photoreaction with spermine has been described. Irradiation of 3'-[32P]-end-labeled DNA fragments for 10-20 min with a germicidal...
The D-JH complex is an intermediate to the complete immunoglobulin heavy-chain V-region gene.
Yaoita, Y, Matsunami, N, Choi, C Y, Sugiyama, H, Kishimoto, T, Honjo, T
We have examined the organization of the immunoglobulin JH segments in three clones derived from a single Abelson murine leukemia virus-transformed cell. Cloning and nucleotide sequence analyses of...
Nakagawa, M, Tsukada, S, Soma, T, Shimizu, Y, Miyake, S, Iwamatsu, A, ...
Kawai, S, Yoshida, M, Segawa, K, Sugiyama, H, Ishizaki, R, Toyoshima, K
The Y73 strain of avian sarcoma virus recently isolated in Japan is defective in replication and is associated with subgroup A leukosis virus (YAV). The virus caused sarcoma but not acute leukosis...
Susceptibility to enteric botulinum colonization of antibiotic-treated adult mice.
The relationship between the indigenous intestinal microflora of adults and their resistance to the enteric botulinum infection of infant botulism was studied. Orogastric challenges of 10(5) type A...
Identification of the human beta-actin enhancer and its binding factor.
Kawamoto, T, Makino, K, Niwa, H, Sugiyama, H, Kimura, S, Amemura, M, ...
An enhancer of the human beta-actin gene and a factor that specifically interacts with it were detected. A mobility shift assay showed that the factor bound to the 25-base-pair sequence (between +759...
Growth potential of Clostridium botulinum in fresh mushrooms packaged in semipermeable plastic film.
Fresh mushrooms (Agaricus bisporus) were inoculated in the stem, gill, or cap with Clostridium botulinum spores. They were placed with uninoculated mushrooms in paper board trays, which were then...
Evaluation of Type A Botulinal Toxin Assays that Use Antitoxin to Crystalline Toxin
Sugiyama, H., Ohishi, I., Dasgupta, B. R.
The type A botulinal toxin assay by the reverse passive hemagglutination procedure which uses antitoxin to crystalline toxin was examined for specificity. The analysis was based on the fact that...
Christian, C N, Daniels, M P, Sugiyama, H, Vogel, Z, Jacques, L, Nelson, P G
There is an increase in the number of acetylcholine (AcCho) receptor aggregates on striated embryonic mouse myotubules when they are cocultured with clonal neuroblastoma-glioma hybrid cells. Medium...
Microbial ecological basis of infant botulism as studied with germfree mice.
The possible role of the indigenous intestinal microflora in the toxicoinfection of human infant botulism was studied with adult germfree mice. Intraintestinal botulinum monoassociation was...
Molecular forms of neurotoxins in proteolytic Clostridium botulinum type B cultures.
A modified purification method was used to isolate the neurotoxin of proteolytic Clostridium botulinum type B strain Lamanna. The preparation was found to be a mixture of two protein forms. They were...
Intraintestinal toxin in infant mice challenged intragastrically with Clostridium botulinum spores.
Conventionally raised suckling mice were injected intragastrically with 10(5) spores of a Clostridium botulinum type A culture. Botulism was not observed, but 80% or more of mice challenged when 8 to...
Role of a Protease in Natural Activation of Clostridium botulinum Neurotoxin
Gupta, Bibhuti R. Das, Sugiyama, H.
All tested proteolytic Clostridium botulinum type A, B, and F strains and certain non-proteolytic B and F cultures produced a protease having trypsin-like substrate specificity; none of the tested...
Muscarinic acetylcholine receptors of the developing retina.
Sugiyama, H, Daniels, M P, Nirenberg, M
Six- and 13-day chicken embryo retinas contain 10 and 320 fmol per mg of protein of specific binding sites for 3-[3H]quinuclidinyl benzilate, a ligand of muscarinic acetylcholine receptors. Most of...
Inhibition of mesangial cell proliferation by E2F decoy oligodeoxynucleotide in vitro and in vivo.
Maeshima, Y, Kashihara, N, Yasuda, T, Sugiyama, H, Sekikawa, T, Okamoto, K, ...
The transcription factor E2F coordinately activates several cell cycle-regulatory genes. We attempted to inhibit the proliferation of mesangial cells in vitro and in vivo by inhibiting E2F activity...
Survival and Outgrowth of Clostridium botulinum Type E Spores in Smoked Fish
Christiansen, Lee N., Deffner, Janet, Foster, E. M., Sugiyama, H.
Chub injected in the loin muscle with 106Clostridium botulinum type E spores were smoked to an internal temperature of 180 F (82.2 C) for 30 min, sealed in plastic bags, and incubated at room...
The rat as an animal model for infant botulism.
Susceptibility to intraintestinal Clostridium botulinum colonization of conventional infant and germfree adult rats is comparable to that of mice. C. botulinum-monoassociated rats pass C. botulinum...
Immunoglobulin gene expression and DNA methylation in murine pre-B cell lines.
Akira, S, Sugiyama, H, Sakaguchi, N, Kishimoto, T
DNA modification accompanying immunoglobulin gene expression was examined in various Abelson murine leukemia virus (A-MuLV)-transformed cell lines, which were able to differentiate from the mu- to...
Yang, X L, Sugiyama, H, Ikeda, S, Saito, I, Wang, A H
Isoguanine (2-hydroxyladenine) is a product of oxidative damage to DNA and has been shown to cause mutation. It is also a potent inducer of parallel-stranded DNA duplex structure. The structure of...
Ito, I, Futai, K, Katagiri, H, Watanabe, M, Sakimura, K, Mishina, M, ...
1. We have explored the effects of targeted disruption of the N-methyl-D-aspartate (NMDA) receptor epsilon 1 or epsilon 2 subunit gene on NMDA receptor-mediated excitatory postsynaptic currents (NMDA...
Nakamura, K, Nukada, T, Haga, T, Sugiyama, H
1. Metabotropic glutamate receptor subtype 1 (mGluR1), when expressed in Xenopus oocytes, activates phosphoinositide-specific phospholipase C (PLC) in a G protein-dependent manner. This reaction...
Hirono, C, Ito, I, Sugiyama, H
1. The intracellular reaction mechanism underlying electrophysiological responses evoked by neurotensin (NT) was studied using Xenopus laevis oocytes injected with poly (A)+ messenger ribonucleic...
Furuya, S, Ohmori, H, Shigemoto, T, Sugiyama, H
1. Intracellular free calcium ([Ca2+]i) was monitored by means of Fura-2 fluorescence measurements in hippocampal cells in primary cultures from newborn rats. 2. In external media containing 200...
Allosteric potentiation of quisqualate receptors by a nootropic drug aniracetam.
Ito, I, Tanabe, S, Kohda, A, Sugiyama, H
1. Allosteric potentiation of the ionotropic quisqualate (iQA) receptor by a nootropic drug aniracetam (1-p-anisoyl-2-pyrrolidinone) was investigated using Xenopus oocytes injected with rat brain...
Sugiyama, H, Kawai, K, Matsunaga, A, Fujimoto, K, Saito, I, Robinson, H, ...
Various oligonucleotides containing 8-methylguanine (m8G) have been synthesized and their structures and thermodynamic properties investigated. Introduction Of M8G into DNA sequences markedly...
Replication of DNA templates containing 5-formyluracil, a major oxidative lesion of thymine in DNA.
Zhang, Q M, Sugiyama, H, Miyabe, I, Matsuda, S, Saito, I, Yonei, S
5-Formyluracil (5-foU) is a major lesion of thymine produced in DNA by ionizing radiation and various chemical oxidants. To assess its biochemical effects on DNA replication, 22mer oligonucleotide...
Substrate recognition by Escherichia coli MutY using substrate analogs.
Chepanoske, C L, Porello, S L, Fujiwara, T, Sugiyama, H, David, S S
The Escherichia coli adenine glycosylase MutY is involved in the repair of 7,8-dihydro-8-oxo-2"-deoxyguanosine (OG):A and G:A mispairs in DNA. Our approach toward understanding recognition and...
Imidazole-imidazole pair as a minor groove recognition motif for T:G mismatched base pairs.
Yang, X L, Hubbard , R B, Lee, M, Tao, Z F, Sugiyama, H, Wang, A H
The T:G mismatched base pair is associated with many genetic mutations. Understanding its biological consequences may be aided by studying the structural perturbation of DNA caused by a T:G base pair...
Improved procedure for crystallization of Clostridium botulinum type A toxic complexes.
Sugiyama, H, Moberg, L J, Messer, S L
A modified protocol for the preparation of botulinum type A toxic crystals is decribed. The essential change was the substitution of chromatography on diethylaminoethyl-Sephadex for the step in which...
The genes for the Clostridium botulinum type G toxin complex are on a plasmid.
Zhou, Y, Sugiyama, H, Nakano, H, Johnson, E A
Clostridium botulinum type G produces a toxin complex that is composed of neurotoxin, hemagglutinin, and nontoxic nonhemagglutinin. The three genes encoding these proteins were closely linked on a...
SYMPOSIUM ON THE BIOLOGY OF BACTERIAL SPORES1
Williams, O. B., Lamanna, Carl, Knaysi, Georges, Wynne, E. Staten, Schmidt, C. F., Curran, Harold R., ...
Colony immunoblot assay of botulinal toxin.
Goodnough, M C, Hammer, B, Sugiyama, H, Johnson, E A
Botulinal neurotoxin in and around colonies of Clostridium botulinum types A, B, and E and of toxigenic Clostridium butyricum was detected by an enzyme-linked immunoassay procedure whereby the toxin...
Zhou, Y, Sugiyama, H, Johnson, E A
Two Clostridium butyricum strains from infant botulism cases produce a toxic molecule very similar to C. botulinum type E neurotoxin. Chromosomal, plasmid, and bacteriophage DNAs of toxigenic and...
Purification and Properties of Clostridium botulinum Type F Toxin
Clostridium botulinum type F toxin of proteolytic Langeland strain was purified. Toxin in whole cultures was precipitated with (NH4)2SO4. Extract of the precipitate was successively chromatographed...
Detection of Clostridium botulinum Toxin by Local Paralysis Elicited with Intramuscular Challenge
Sugiyama, H., Brenner, Shelley L., Dasgupta, B. R.
Clostridium botulinum toxin can be identified by a characteristic, acute local paralysis that follows its injection into the gastrocnemius (“calf” muscle) of mice The local botulism can be...
Simplified purification method for Clostridium botulinum type E toxin.
Clostridium botulinum type E toxin was purified in three chromatography steps. Toxin extracted from cells was concentrated by precipitation and dissolving in a small volume of citrate buffer. When...
Production of toxin by Clostridium botulinum type A strains cured by plasmids.
Weickert, M J, Chambliss, G H, Sugiyama, H
Twelve strains of Clostridium botulinum type A and seven strains of Clostridium sporogenes were screened for plasmids by agarose gel electrophoresis of cleared lysates of cells from 5 ml of...
Acute toxicity of aminoglycoside antibiotics as an aid in detecting botulism.
Wang, Y C, Burr, D H, Korthals, G J, Sugiyama, H
Gentamicin sulfate or neomycin sulfate injected intraperitoneally into 24- to 27-g mice at a dose of 6.2 mg per mouse elicited botulism-like responses in less than 30 min, but a dose of 3.1 mg per...
Affinity chromatography purification of type A botulinum neurotoxin from crystalline toxic complex.
Type A botulinum neurotoxin was purified from toxic crystals by adsorption to p-aminophenyl-beta-D-thiogalactopyranoside coupled to CH-Sepharose 4B. At pH 6.3, the toxic complex was held by the...
Toxicity in culture fluids of several Clostridium botulinum type A strains was assayed in mice and converted to weight equivalent. The toxin-related antigen in the samples was quantitated by a...
Nature of intracellular type A botulinum neurotoxin.
The neurotoxin in cells of young Clostridium botulinum type A culture was extracted with lysozyme. Highly purified neurotoxin preparation, obtained by processing the extract in two chromatographic...
Boticinogeny and Actions of the Bacteriocin1
Anastasio, Kathryn L., Soucheck, J. A., Sugiyama, H.
The bacteriocin, boticin E, was produced by only a few strains of those clostridia which are nontoxigenic but otherwise identical to Clostridium botulinum type E. Boticin preparations from four...
Brown, George W., King, Gretchen, Sugiyama, H.
When logarithmically growing cultures of Clostridium botulinum types A and E are treated with penicillin in a liquid medium containing 8% polyethylene glycol, protoplast-like spherical bodies are...
Fine Structure of Protoplasts and L-Forms of Clostridium botulinum Types A and E1
Brown, George W., Pate, J. L., Sugiyama, H.
Spherical bodies, that are obtained by adding penicillin and lysozyme to Clostridium botulinum types E and A cultures which are growing in an osmotically stabilized medium, are shown to be...
Bott, Thomas L., Johnson, Jodie, Foster, E. M., Sugiyama, H.
Bottom and shoreline sediments of Green Bay, northern Lake Michigan, and rivers of the Green Bay drainage basin, as well as soils of the surrounding land mass, were examined for Clostridium botulinum...
Rabbit Ileal Loop Response to Strains of Clostridium perfringens1
Duncan, Charles L., Sugiyama, H., Strong, Dorothy H.
The ligated loop of the rabbit intestine was investigated as a possible experimental model for the study of Clostridium perfringens food poisoning. The method of preparation of the challenge inoculum...
Comparison of toxins of Clostridium butyricum and Clostridium botulinum type E.
The toxin of Clostridium butyricum strains isolated from two infants with botulism is neutralized by antitoxin for type E botulinum toxin. This toxin and that of a C. botulinum type E strain were...
Sugiyama, H, Prather, J L, Woller, M J
Adult mice, made susceptible to Clostridium botulinum by feedings of metronidazole, were immobilized with an anesthetic and held for 30 min in isolators in which a fine powder of lyophilized pathogen...
Inactivation of botulinum and tetanus toxins by chelators.
Bhattacharyya, S D, Sugiyama, H
Purified type A botulinum toxin of about 10(6) mouse 50% lethal doses per ml was greater than 99.9% inactivated when incubated at pH 7.4 for 30 min at 37 degrees C in 20 mM 1,10-phenanthroline (PTL)...
Conventional adult mice were pretreated with metronidazole to make their intestinal tracts receptive to colonization by Clostridium botulinum. These mice, in groups of 10, were fed 0 (controls),...
Leukocytic Response in Monkeys Challenged with Staphylococcal Enterotoxin
Sugiyama, H. (University of Chicago, Chicago, Ill.), and E. M. McKissic, Jr. Leukocytic response in monkeys challenged with staphylococcal enterotoxin. J. Bacteriol. 92:349–352. 1966—The feeding...
Saito, I, Sugiyama, H, Matsuura, T, Ueda, K, Komano, T
A new procedure for T specific cleavage of DNA fragments utilizing photoreaction with spermine has been described. Irradiation of 3'-[32P]-end-labeled DNA fragments for 10-20 min with a germicidal...
The D-JH complex is an intermediate to the complete immunoglobulin heavy-chain V-region gene.
Yaoita, Y, Matsunami, N, Choi, C Y, Sugiyama, H, Kishimoto, T, Honjo, T
We have examined the organization of the immunoglobulin JH segments in three clones derived from a single Abelson murine leukemia virus-transformed cell. Cloning and nucleotide sequence analyses of...
Nakagawa, M, Tsukada, S, Soma, T, Shimizu, Y, Miyake, S, Iwamatsu, A, ...
Kawai, S, Yoshida, M, Segawa, K, Sugiyama, H, Ishizaki, R, Toyoshima, K
The Y73 strain of avian sarcoma virus recently isolated in Japan is defective in replication and is associated with subgroup A leukosis virus (YAV). The virus caused sarcoma but not acute leukosis...
Susceptibility to enteric botulinum colonization of antibiotic-treated adult mice.
The relationship between the indigenous intestinal microflora of adults and their resistance to the enteric botulinum infection of infant botulism was studied. Orogastric challenges of 10(5) type A...
Identification of the human beta-actin enhancer and its binding factor.
Kawamoto, T, Makino, K, Niwa, H, Sugiyama, H, Kimura, S, Amemura, M, ...
An enhancer of the human beta-actin gene and a factor that specifically interacts with it were detected. A mobility shift assay showed that the factor bound to the 25-base-pair sequence (between +759...
Growth potential of Clostridium botulinum in fresh mushrooms packaged in semipermeable plastic film.
Fresh mushrooms (Agaricus bisporus) were inoculated in the stem, gill, or cap with Clostridium botulinum spores. They were placed with uninoculated mushrooms in paper board trays, which were then...
Evaluation of Type A Botulinal Toxin Assays that Use Antitoxin to Crystalline Toxin
Sugiyama, H., Ohishi, I., Dasgupta, B. R.
The type A botulinal toxin assay by the reverse passive hemagglutination procedure which uses antitoxin to crystalline toxin was examined for specificity. The analysis was based on the fact that...
Christian, C N, Daniels, M P, Sugiyama, H, Vogel, Z, Jacques, L, Nelson, P G
There is an increase in the number of acetylcholine (AcCho) receptor aggregates on striated embryonic mouse myotubules when they are cocultured with clonal neuroblastoma-glioma hybrid cells. Medium...
Microbial ecological basis of infant botulism as studied with germfree mice.
The possible role of the indigenous intestinal microflora in the toxicoinfection of human infant botulism was studied with adult germfree mice. Intraintestinal botulinum monoassociation was...
Molecular forms of neurotoxins in proteolytic Clostridium botulinum type B cultures.
A modified purification method was used to isolate the neurotoxin of proteolytic Clostridium botulinum type B strain Lamanna. The preparation was found to be a mixture of two protein forms. They were...
Intraintestinal toxin in infant mice challenged intragastrically with Clostridium botulinum spores.
Conventionally raised suckling mice were injected intragastrically with 10(5) spores of a Clostridium botulinum type A culture. Botulism was not observed, but 80% or more of mice challenged when 8 to...
Role of a Protease in Natural Activation of Clostridium botulinum Neurotoxin
Gupta, Bibhuti R. Das, Sugiyama, H.
All tested proteolytic Clostridium botulinum type A, B, and F strains and certain non-proteolytic B and F cultures produced a protease having trypsin-like substrate specificity; none of the tested...
Muscarinic acetylcholine receptors of the developing retina.
Sugiyama, H, Daniels, M P, Nirenberg, M
Six- and 13-day chicken embryo retinas contain 10 and 320 fmol per mg of protein of specific binding sites for 3-[3H]quinuclidinyl benzilate, a ligand of muscarinic acetylcholine receptors. Most of...
Inhibition of mesangial cell proliferation by E2F decoy oligodeoxynucleotide in vitro and in vivo.
Maeshima, Y, Kashihara, N, Yasuda, T, Sugiyama, H, Sekikawa, T, Okamoto, K, ...
The transcription factor E2F coordinately activates several cell cycle-regulatory genes. We attempted to inhibit the proliferation of mesangial cells in vitro and in vivo by inhibiting E2F activity...
Survival and Outgrowth of Clostridium botulinum Type E Spores in Smoked Fish
Christiansen, Lee N., Deffner, Janet, Foster, E. M., Sugiyama, H.
Chub injected in the loin muscle with 106Clostridium botulinum type E spores were smoked to an internal temperature of 180 F (82.2 C) for 30 min, sealed in plastic bags, and incubated at room...
The rat as an animal model for infant botulism.
Susceptibility to intraintestinal Clostridium botulinum colonization of conventional infant and germfree adult rats is comparable to that of mice. C. botulinum-monoassociated rats pass C. botulinum...
Immunoglobulin gene expression and DNA methylation in murine pre-B cell lines.
Akira, S, Sugiyama, H, Sakaguchi, N, Kishimoto, T
DNA modification accompanying immunoglobulin gene expression was examined in various Abelson murine leukemia virus (A-MuLV)-transformed cell lines, which were able to differentiate from the mu- to...
Ito, I, Futai, K, Katagiri, H, Watanabe, M, Sakimura, K, Mishina, M, ...
1. We have explored the effects of targeted disruption of the N-methyl-D-aspartate (NMDA) receptor epsilon 1 or epsilon 2 subunit gene on NMDA receptor-mediated excitatory postsynaptic currents (NMDA...
Nakamura, K, Nukada, T, Haga, T, Sugiyama, H
1. Metabotropic glutamate receptor subtype 1 (mGluR1), when expressed in Xenopus oocytes, activates phosphoinositide-specific phospholipase C (PLC) in a G protein-dependent manner. This reaction...
Hirono, C, Ito, I, Sugiyama, H
1. The intracellular reaction mechanism underlying electrophysiological responses evoked by neurotensin (NT) was studied using Xenopus laevis oocytes injected with poly (A)+ messenger ribonucleic...
Furuya, S, Ohmori, H, Shigemoto, T, Sugiyama, H
1. Intracellular free calcium ([Ca2+]i) was monitored by means of Fura-2 fluorescence measurements in hippocampal cells in primary cultures from newborn rats. 2. In external media containing 200...
Allosteric potentiation of quisqualate receptors by a nootropic drug aniracetam.
Ito, I, Tanabe, S, Kohda, A, Sugiyama, H
1. Allosteric potentiation of the ionotropic quisqualate (iQA) receptor by a nootropic drug aniracetam (1-p-anisoyl-2-pyrrolidinone) was investigated using Xenopus oocytes injected with rat brain...
Yang, X L, Sugiyama, H, Ikeda, S, Saito, I, Wang, A H
Isoguanine (2-hydroxyladenine) is a product of oxidative damage to DNA and has been shown to cause mutation. It is also a potent inducer of parallel-stranded DNA duplex structure. The structure of...
AT8--1-12--5-1, an Abelson virus-transformed immature B-cell line, produced truncated mu-chains. Sequencing analysis of the mu-expressed allele revealed that the variable region was an out-of-frame...
Minami, Y, Sakato, N, Komori, T, Kishimoto, S, Sugiyama, H
To raise monoclonal antibodies that specifically recognize the heavy chain variable region of MOPC141 myeloma protein (VH141), which belongs to VHQ52 family, rats were immunized with Fd'-conjugated...
Sugiyama, H, Minami, Y, Komori, T, Sakato, N, Kishimoto, S
We raised anti-VH315 antibodies by immunization of rabbits with VH315 fragments, the variable portions of the immunoglobulin heavy chains of MOPC315 myeloma protein. Inhibition radioimmunoassay using...
Implantation of endovascular stents for the obstructive right ventricular outflow tract
Sugiyama, H, Williams, W, Benson, L N
Objectives: To evaluate the effectiveness and long term outcomes of catheter intervention for obstructive conduits between the right ventricle and pulmonary arteries.