H. Wolf

Publication List Details

Period

1967 - 9999

Number

370

Co-Authors

Growth of Electrically Charged Soot Particles in Flames, (9999)

Homann,K. H., Stroefer,E., Wolf,H.

A part of the soot particles in fuel-rich hydrocarbon flames carry an electric charge. Charges of both signs occur on soot particles in the same flame. Through a molecular beam sampling system,...

First sequence-confirmed case of infection with the new influenza A(H1N1) strain in Germany (2009)

Melzl, H., Wenzel, J. J., Kochanowski, B., Feierabend, K., Kreuzpaintner, B., Kreuzpaintner, E., ...

Here, we report on the first sequence-confirmed case of infection with the new influenza A(H1N1) virus in Germany. Two direct contacts of the patient were laboratory-confirmed as cases and...

Adverse drug reactions to tocolytic treatment for preterm labour: prospective cohort study (2009)

De Heus, R., Mol, B.W., Van Geijn, H.P., Gyselaers, W.J., Hanssens, M., ...

Objective To evaluate the incidence of serious maternal complications after the use of various tocolytic drugs for the treatment of preterm labour in routine clinical situations. Design Prospective...

Rapid chemiluminescence biosensing of microcystin-LR (2009)

Lindner, P., Molz, R., Yacoub-George, E., Wolf, H.

A rapid immunoassay for sensitive detection of microcystin-LR using a portable chemiluminescence multichannel immunosensor (CL-MADAG) was developed. The sensor device is based on a capillary ELISA...

Density and Absolute Salinity of the Baltic Sea 2006–2009 (2009)

R. Feistel, S. Weinreben, H. Wolf, S. Seitz, P. Spitzer, B. Adel, ...

The brackish water of the Baltic Sea is a mixture of ocean water from the Atlantic/North Sea with fresh water from various rivers draining a large area of lowlands and mountain ranges. The...

Pulsed stress behavior of flexible thick film resistors (2008)

Bonfert, D., Wolf, H., Gieser, H., Klink, G., Bock, K., Svasta, P., ...

In order to investigate the behavior for very high current densities on polymer resistors on flexible substrates, a pulsed measurement technique was applied. The analytical test technique of...

Hair plate mechanoreceptors associated with body segments are not necessary for three-dimensional path integration in desert ants, Cataglyphis fortis (2007)

Wittlinger, M, Wolf, H, Wehner, R

In formicine ants, the hair fields associated with the neck and the petiole (alitrunk–petiole and petiole–gaster joints) have long been established to function in graviception. Here, we examine a...

Investigations with the capacitive coupled TLP on package and wafer-level (2007)

Wolf, H., Gieser, H.

CDM is the major root cause for ESD failures in a modern production and test environment. CDM stress testing is well established for product qualification. Although specified as CDM failure voltage,...

Automated 10-channel capillary chip immunodetector for biological agents detection (2007)

Yacoub-George, E., Hell, W., Meixner, L., Wenninger, F., Bock, K., Lindner, P., ...

The automated 10-channel capillary chip immunodetector (10K-IDWG) is a prototype, which has been developed for automatically operated biological agents (BA) point detection. The current technology...

The desert ant odometer: a stride integrator that accounts for stride length and walking speed. (2007)

Wittlinger, M, Wehner, R, Wolf, H

Desert ants, Cataglyphis, use path integration as a major means of navigation. Path integration requires measurement of two parameters, namely, direction and distance of travel. Directional...

Investigating the CDM susceptibility of ICs at package and wafer level by capacitive coupled TLP (2007)

Wolf, H., Gieser, H., Walter, D.

The method of the Capacitive Coupled Transmission Line Pulsing (CC-TLP) is applied to a product IC at package level and for the first time at wafer level. The investigated product showed a field...

Survey on very fast TLP and ultra fast repetitive pulsing for characterization in the CDM-domain (2007)

Gieser, H.A., Wolf, H.

Charged device model pulses may be less than 1 ns wide with peak currents exceeding 10 A. They are a true challenge for the ESD protection of advanced technologies with shrinking safety margins. This...

Transmission line pulse stress on thick film resistors (2007)

Bonfert, D., Wolf, H., Gieser, H., Svasta, P., Romanescu, A., Cazacu, E.

One of the most important issues of resistors properties is the value stability under different electrical and non electrical influences. Mechanical and/or thermal stress together with the electrical...

The ant odometer: stepping on stilts and stumps. (2006)

Wittlinger, M, Wehner, R, Wolf, H

Desert ants, Cataglyphis, navigate in their vast desert habitat by path integration. They continuously integrate directions steered (as determined by their celestial compass) and distances traveled,...

SOL-ACES: Auto-calibrating EUV/UV spectrometers for measurements onboard the International Space Station (2006)

Schmidtke, G., Brunner, R., Eberhard, D., Halford, B., Klocke, U., Knothe, M., ...

The SOL-ACES experiment is prepared to be flown with the ESA SOLAR payload to the International Space Station as planned for the Shuttle mission E1 (Columbus) in August 2006. Four grazing incidence...

Transient analysis of ESD protection elements by time domain transmission using repetitive pulses (2006)

Wolf, H., Gieser, H., Stadler, W., Wilkening, W., Rose, P., Qu, N.

This paper describes a test method which allows the investigation of the transient switching behavior of ESD-protection elements with very high transient resolution within the first nanoseconds. It...

ESD susceptibility of submicron air gaps (2006)

Wolf, H., Gieser, H., Bonfert, D., Hauser, M.

This work describes the investigation of the ESD susceptibility of submicron air gaps which are used e.g. in filter devices. The breakdown behaviour of the air gaps is analyzed in both the HBM (Human...

Modellierung von Prüfsystemen und Schutzelementen zur Kompaktsimulation von elektrostatischen Entladungen an integrierten Schaltungen (2006)

Wolf, H.

Elektrostatische Entladungen (ESD, Electro Static Discharge) sind eine der Hauptausfallursachen von integrierten Schaltungen. Integrierte ESD Schutzstrukturen reduzieren dieses Risiko. Fehlerhafte...

Transient-induced latch-up test setup for wafer-level and package-level (2006)

Bonfert, D., Gieser, H., Wolf, H., Frank, M., Konrad, A., Schulz, J.

Latch-up triggered by an impulse of short duration, is one root cause for field failures of CMOS devices. Standard tests. like JEDEC 78, which apply quasi-static overvoltage and overcurrent may fail...

Sensitivity of spontaneous baroreflex control of the heart and hemodynamic parameters are not influenced by the menstrual cycle (2006)

Vollebregt, K.C., Seesing, L., Rang, S., Boer, K., Wolf, H.

Objective: The purpose of this study was to evaluate if hemodynamic parameters and sympathetic activity vary between the follicular and luteal phase of the menstrual cycle before using sympathetic...

Increased sympathetic activity present in early hypertensive pregnancy is not lowered by plasma volume expansion (2006)

Metsaars, W.P., Ganzevoort, J.W., Karemaker, J.M., Rang, S., Wolf, H.

Objective: To evaluate whether sympathetic activity is increased in early-onset hypertensive pregnancy and whether this can be influenced by management with plasma volume expansion. Methods: The...

Prediction of maternal complications and adverse infant outcome at admission for temporizing management of early-onset severe hypertensive disorders of pregnancy (2006)

Ganzevoort, J.W., Rep, A., Vries, J.I.P., Bonsel, G.J., Wolf, H.

Objective: We explored the association between clinical parameters at admission and the subsequent development of major maternal complications or adverse infant outcome in women with hypertensive...

ESD susceptibility of thick film chip resistors by means of transmission line pulsing (2006)

Bonfert, D., Wolf, H., Gieser, H., Stocker, A.

The change in resistance (dR) due to an applied high voltage pulse is a measure of the electrostatic discharge susceptibility of a resistor. The influence of the pulse width and height on the...

Desert ants compensate for navigation uncertainty. (2005)

Wolf, H, Wehner, R

During foraging trips, desert ants Cataglyphis fortis do not rely only on their well-studied path integration system, they also use olfactory cues when approaching a familiar food source. When a wind...

ESD-Schutztransistor (2005)

Wolf, H., Wilkening, W., Mettler, S.

WO 200042658 A UPAB: 20010110 NOVELTY - The highly doped n-emitter region (36) is not short-circuited to the highly-doped p-base region (34). USE - To design electrostatic discharge protection...

A dedicated TLP set-up to investigate the ESD robustness of RF elements and circuits (2005)

Wolf, H., Gieser, H., Soldner, W., Goßner, H.

This work describes the development of a combined RF-TLP test set-up. It alternates between pulsed high current characterization and scattering parameter measurements up to 10 GHz in order to...

Capacitively coupled transmission line pulsing cc-TLP - a traceable and reproducible stress method in the CDM-domain (2005)

Wolf, H., Gieser, H., Stadler, W., Wilkening, W.

This paper describes a new test method called capacitively coupled transmission line pulsing cc-TLP. It is applied to different test circuits which were mounted on specially designed package...

Transient latch-up: Experimental analysis and device simulation (2005)

Bargstädt-Franke, S., Stadler, W., Esmark, K., Streibl, M., Domanski, K., Gieser, H., ...

A set-up consisting of at least one pulse generator with baseline functionality was used for transient latch-up (TLU) investigations. Dependencies of the TLU sensitivity of test structures on the...

Test circuits for fast and reliable assessment of CDM robustness of I/O stages (2005)

Stadler, W., Esmark, K., Reynders, K., Zubeidat, M., Graf, M., Wilkening, W., ...

CDM hardening during the development of technology, devices, libraries and finally products differs significantly from the process well-established for HBM. This paper introduces a method on the...

Phenotyping of N-acetyltransferase type 2 by caffeine from uncontrolled dietary exposure (2004)

Jetter, A., Kinzig-Schippers, M., Illauer, M., Hermann, R., Erb, K., Borlak, J., ...

BACKGROUND AND OBJECTIVE: The standard approach for phenotyping of the human arylamine N-acetyltransferase 2 (NAT2) uses urinary caffeine metabolite ratios after a caffeine test dose taken in after...

Characterization and modeling of transient device behavior under CDM ESD stress (2004)

Willemen, J., Andreini, A., Heyn, V. De, Esmark, K., Etherton, M., Gieser, H., ...

Very-fast high-current pulses that occur during charged device model (CDM) ESD events lead to transient voltage overshoots in forward- and reverse-biased pn-junctions, called forward recovery and...

Internal behavior of BCD ESD protection devices under TLP and very-fast TLP stress (2004)

Blaho, M., Zullino, L., Wolf, H., Stella, R., Andreini, A., Gieser, H.A., ...

BCD electrostatic discharge (ESD) protection npn devices with different layout variations are analyzed experimentally an by device simulation. The device internal thermal and free carrier density...

Methods and progress in the conservation of elm genetic resources in Europe (2004)

Collin, E., Rusanen, M., Ackzell, L., Bohnens, J., De Aguiar, A., Diamandis, S., ...

The progress made in the conservation of European elm genetic resources since the 1st International Elm Conference is reviewed, and the complementarity of in situ and ex situ methods is discussed....

Electrical characterisation of a power SO-package in the context of electrostatic discharge (2003)

Dörr, I., Gieser, H.A., Sommer, G., Wolf, H., Wilkening, W., Willemen, J., ...

The electrostatic discharge (ESD) sensitivity of integrated circuits with respect to the charged device model depends strongly on the package. Electrical characteristics of a package are, if at all...

Investigation of ESD protection elements under high current stress in CDM-like time domain using backside laser interferometry (2003)

Bychikhin, S., Dubec, V., Litzenberger, M., Pogany, D., Gornik, E., Groos, G., ...

Switching dynamics and current flow homogeneity under very-fast TLP (vf-TLP) stress is investigated in smart power and CMOS technology ESD protection devices by means of optical transient...

Morphometry of the corpus callosum in patients with questionable and mild dementia (2002)

Hensel,A., Wolf,H., Kruggel,F., Riedel-Heller,S. G., Nikolaus,C., Arendt,T., ...

Previous studies have shown a reduction in corpus callosum area in advanced Alzheimer's disease, but it is unclear whether callosal atrophy is present in the transitional phase between health and the...

Morphometry of the corpus callosum in patients with questionable and mild dementia (2002)

Hensel,A., Wolf,H., Kruggel,F., Riedel-Heller,S. G., Nikolaus,C., Arendt,T., ...

Previous studies have shown a reduction in corpus callosum area in advanced Alzheimer's disease, but it is unclear whether callosal atrophy is present in the transitional phase between health and the...

Theta-power differences in patients with mild cognitive impairment under rest condition and during haptic tasks (2002)

Grunwald,M., Busse,F., Hensel,A., Riedel-Heller,S., Kruggel,F., Arendt,T., ...

The aim of this study was to investigate spectral EEG theta-power during perceptive-cognitive demands in age-homogeneous groups of subjects with mild cognitive impairment (MCI), mild dementia (MDE),...

Theta-power differences in patients with mild cognitive impairment under rest condition and during haptic tasks (2002)

Grunwald,M., Busse,F., Hensel,A., Riedel-Heller,S., Kruggel,F., Arendt,T., ...

The aim of this study was to investigate spectral EEG theta-power during perceptive-cognitive demands in age-homogeneous groups of subjects with mild cognitive impairment (MCI), mild dementia (MDE),...

Morphometry of the corpus callosum in patients with questionable and mild dementia (2002)

Hensel, A., Wolf, H., Kruggel, F., Riedel-Heller, S. G., Nikolaus, C., Arendt, T., ...

Previous studies have shown a reduction in corpus callosum area in advanced Alzheimer's disease, but it is unclear whether callosal atrophy is present in the transitional phase between health and the...

Theta-power differences in patients with mild cognitive impairment under rest condition and during haptic tasks (2002)

Grunwald, M., Busse, F., Hensel, A., Riedel-Heller, S., Kruggel, F., Arendt, T., ...

The aim of this study was to investigate spectral EEG theta-power during perceptive-cognitive demands in age-homogeneous groups of subjects with mild cognitive impairment (MCI), mild dementia (MDE),...

Investigation of long throw PVD of titanium films from polycrystalline targets with texture (2002)

Wolf, H., Streiter, R., Tirschler, W., Giegengack, H., Urbansky, N., Gessner, T.

The influence of target properties on the deposition of titanium films by long throw sputtering has been studied using Monte Carlo simulation. The precise knowledge and consideration of existing...

Chemiluminescence multichannel immunosensor for biodetection (2002)

Yacoub-George, E., Meixner, L., Scheithauer, W., Koppi, A., Drost, S., Wolf, H., ...

An improved portable detector for biological compounds, the chemiluminescence multichannel immunosensor has been developed and characterised. The device is based on a capillary ELISA technique in...

ESD circuit simulation for the prevention of ESD failures. Application to products in a 0.18 µm CMOS technology (2002)

Wolf, H., Gieser, H., Stadler, W., Esmark, K.

This paper describes the ESD circuit simulation of MOS transistors processed in a 0.18 µm CMOS technology. The extended model simulates the breakdown between the external base and the emitter...

ESD-Schutzvorrichtung für integrierte Schaltungen (2002)

Wilkening, W., Wolf, H.

NOVELTY - A highly doped N-region (12,18) contains a second connecting electrode (16), while a weakly doped P-region (20) is adjacent to the highly doped P-region (10) and a weakly doped N-region...

Theta-power differences in patients with mild cognitive impairment under rest condition and during haptic tasks (2002)

Grunwald, M., Busse, F., Hensel, A., Riedel-Heller, S., Kruggel, F., Arendt, T., ...

The aim of this study was to investigate spectral EEG theta-power during perceptive-cognitive demands in age-homogeneous groups of subjects with mild cognitive impairment (MCI), mild dementia (MDE),...

ESD-level circuit simulation impact of interconnect RC-delay on HBM and CDM behavior: (Reprinted from Electrical Overstress/Electrostatic Discharge Symposium Proceedings, 2000) (2002)

Mergens, M.P.J., Wilkening, W., Kiesewetter, G., Mettler, S., Wolf, H., Hieber, E., ...

An extraction method for the distributed, parasitic RC-elements of MOS single- and multi-fingers is introduced by deducing a rule of thumb for an effective poly resistance Reff. The lumped RC element...

Pinpointing food sources: olfactory and anemotactic orientation in desert ants, Cataglyphis fortis. (2000)

Wolf, H, Wehner, R

Desert ants, Cataglyphis fortis, search for a repeatedly visited food source by employing a combined olfactory and anemotactic orientation strategy (in addition to their visually based...

Defect complexes formed with Ag atoms in CDTE, ZnTe, and ZnSe (2000)

Wolf, H, Filz, T, Ostheimer, V, Hamann, J, Lany, S, Wichert, T

Using the radioactive acceptor 111Ag for perturbed gg-angular correlation (PAC) spectroscopy for the first time, defect complexes formed with Ag are investigated in the II-VI semiconductors CdTe,...

Identification of Ag-acceptors in $^{111}Ag / ^{111}Cd$ doped ZnTe and CdTe (2000)

Hamann, J, Burchard, A, Deicher, M, Filz, T, Lany, S, Ostheimer, V, ...

Nominally undoped ZnTe and CdTe crystals were implanted with radioactive 111Ag, which decays to 111Cd, and investigated by photoluminescence spectroscopy (PL). In ZnTe, the PL lines caused by an...

Verfahren zum Nachweis von Prionen sowie eine Vorrichtung hierzu (2000)

Hauck, S., Wolf, H., Drost, S.

A method for determining the presence of spongiform encephalopathy (SE) causing organisms in a biological material containing pathological and physiological prion isoforms, comprises: (a) digesting...

Analysis of lateral DMOS power devices under ESD stress conditions (2000)

Mergens, M.P.J., Wilkening, W., Mettler, S., Wolf, H., Stricker, A., Fichtner, W.

The physical mechanisms specific for 40 V LDMOS power transistors under ESD stress (gate grounded/coupled) are investigated in detail by transmission line pulse (TLP) measurements, human body model...

Modular approach of a high current MOS compact model for circuit-level ESD simulation including transient gate-coupling behaviour (2000)

Mergens, M., Wilkening, W., Mettler, S., Wolf, H., Fichtner, W.

A novel modular strategy for highly flexible modeling of ESD-capable MOS compact models is introduced. This high current MOS model comprises the important gate-coupling effect and an approximated...

Liberal Diagnosis and Treatment of Intrauterine Infection Reduces Early-Onset Neonatal Group B Streptococcal Infection but not Sepsis by Other Pathogens (2000)

H. Wolf, B. J. Smit, L. Spanjaard, A. H. Adriaanse

Objective: Comparison of the incidence and case fatality of early-onset group B streptococcus sepsis and sepsis caused by other pathogens in neonates after change of management of intrauterine...

Liberal Diagnosis and Treatment of Intrauterine Infection Reduces Early-Onset Neonatal Group B Streptococcal Infection but not Sepsis by Other Pathogens (2000)

H. Wolf, B. J. Smit, L. Spanjaard, A. H. Adriaanse

Objective: Comparison of the incidence and case fatality of early-onset group B streptococcus sepsis and sepsis caused by other pathogens in neonates after change of management of intrauterine...

Zeitlich hochaufgelöste Gasanalytik. Anwendung der Infrarotdioden-Laserspektroskopie am Beispiel von Verbrennungsprozessen (1998)

Grisar, R., Riedel, W.J., Tacke, M., Wolf, H., Wagner, E.

Infrared diode laser spectroscopy enables dynamic exhaust gas analysis with millisecond time response. A spectroscopic analyser for the simultaneous measurement of two exhaust gas components at two...

Hierarchical mask data preparation and special fracturing techniques in MGS (1998)

Bürger, B., Bätz, U., Kunze, K., Wolf, H.

MGS is a layout postprocessor software including a fracturing engine for Leica's ZBA e-beam writers. It is installed in mask shops, fabs, research institutes and universities as the data preparation...

Dynamic exhaust gas analysis. A tool to study and to improve engine management (1998)

Wagner, E., Grisar, R., Riedel, W.J., Tacke, M., Wolf, H.

Infrared diode laser spectroscopy enables dynamic exhaust gas analysis with millisecond time response. A spectroscopic analyser for the simultaneous measurement of two exhaust gas components at two...

Reduced Patient Dose and Improved Image Quality in Computed Tomography Examinations with On-Line Anatomically Adapted Tube Current Modulation (1998)

Hentschel, D., Klingenbeck-Regn, K., Popescu, S., Suess, C., Wolf, H., Kalender, W.

A new concept of an on-line anatomically adapted modulation (OAC) of the tube current has been investigated and compared with the pre-programmed sinusoidal modulation (PSC), by measuring Computed...

Ultrasonic Measurement of Hardening Depth (1997)

Reddy, K.M., Theiner, W., Wolf, H.

Induction and flame hardening are standard heat treatment procedures for generating hard cases in steels, typically thicker than 1 mm. Examples are crank shafts for diesel engines or rollers used in...

A silicon micromachined open tubular reactor for immunosensor applications (1996)

Woias, P., Richter, M., Yacoub-George, E., Wolf, H., Abel, T.

In this publication, a micromachined open tubular reactor is presented, which has been developed for the rapid and reliable performance of heterogeneous immunoassays in a miniatunzed chemical...

Process Integrated Nondestructive Testing for Evaluation of Hardness (1996)

Kröning, M., Theiner, W., Kern, R., Bucher, B., Meyer, R., Wolf, H.

With the help of new PINT sensors, product characteristics are evaluated which otherwise could only be determined so far by time-consuming and cost-intensive testing. These sensors would permit...

A miniaturized ISFET-ELISA system with a pretreated fused silica capillary as reaction cartridge (1996)

Yacoub-George, E., Wolf, H., Koch, S., Woias, P.

A universal ELISA-based analytical system is described, which consists of a flow-through set-up with a pretreated fused silica capillary as a reaction cartridge and an ISFET array as a detector. The...

A micromachined open tubular reactor for immunosensor applications (1996)

Woias, P., Richter, M., Yacoub-George, E., Wolf, H., Abel, T.

A micromachined open tubular reactor is presented, which was developed with special focus on the application in heterogeneous immunoassays such as the Enzyme Linked Immunosorbent Assay (ELISA). In...

Aufbau eines Rechteckimpuls-Generators nach dem Transmission-Line-Prinzip mit verschiedenen Pulsdauern und Belastung von Halbleiter-Schutzstrukturen (1995)

Mußhoff, C., Wolf, H., Gieser, H.

Die Entwicklung und die Anwendung eines Rechteckimpulsgenerators für hohe Impulsströme ist Gegenstand dieses Beitrags. Die Impulse variabler Dauer werden mittels Transmission-Line und Relais...

Structure of Hydrophilic Self-Assembled Monolayers: A Combined Scanning Tunneling Microscopy and Computer Simulation Study (1994)

Sprik, M., Delamarche, E., Michel, B., Roethlisberger, U., Klein, M. L., Wolf, H., ...

Monolayers of thiols self-assembled on Au (111) can be made hydrophilic by functionalizing the surface with polar endgroups. The authors present scanning tunneling microscopy (STM) images of 2 such...

Oral administration of a bacterial immunomodulator enhances murine intestinal lamina propria and Peyer's patch lymphocyte traffic to the lung: possible implications for infectious disease prophylaxis and therapy (1993)

Ruedl, Ch., Albin, B., Böck, G., Wick, G., Wolf, H., Doria, G.

LW50020, a bacterial immunomodulator, is a preparation consisting of seven bacteria, commonly causing respiratory disease. When given orally, LW50020 has been shown to enhance the host defense of the...

Analysis of trace components in automotive exhaust gas by TDLS (1992)

Riedel, W.J., Grisar, R., Klocke, U., Knothe, M., Wolf, H., Schottka, P., ...

A four-component tunable diode laser spectroscopy system for highly sensitive real time car exhaust gas analysis with four diode lasers operated simultaneously in frequency multiplex is reported on....

Photoemission study on the valence bands of DMe-DCNQI derived charge-transfer salts (1991)

Schmeißer, D., Gonzales, A., Von Schütz, J., Wachtel, H., Wolf, H.

The electronic structure of crystalline needles of the 2,5-Dimethyl substituted (DCNQI)2M (M = Cu, Rb, Cs) radical-anion salts, prepared in situ, is determined by photoelectrons spectroscopy using...

Time-resolved exhaust gas analysis by infrared diode laser spectroscopy (1991)

Klocke, U., Riedel, W.J., Wolf, H.

We report on a fast exhaust gas analysis system, based on infrared spectroscopy of a singel absorption line by pulse-operated diode lasers and a newly designed gas sampling system. Otto and Diesel...

Monochromatoranordnung. (1991)

Riedel, W., Knothe, M., Grisar, R., Wolf, H., Preier, H.M.

Eine Monochromatoranordnung verfuegt ueber einen Monochromator (9) und eine Umlenkeinrichtung (2), die mit Hilfe von vier Umlenkspiegeln (10, 11, 17, 18) den Austrittslichtstrahl (13) eines in einen...

Low-frequency amplitude-noise characteristics of lead-salt diode lasers fabricated by molecular-beam-epitaxy. (1991)

Fischer, H., Halford, B., Tacke, M., Wolf, H.

Low-frequency amplitude-noise measurements for several lead-salt diode lasers fabricated by molecular-beam-epitaxy are presented. The influence of current fluctuations of the current source and of...

Infrared diode laser exhaust gas analyser for trace components (1991)

Bessey, E., Schottka, P., Pelz, N., Grisar, R., Klocke, U., Knothe, M., ...

We report on a four-component TDLS system for highly sensitive real time car exhaust gas analysis, based on infrared diode laser absorption spectroscopy. Four lead-salt diode lasers, one for each gas...

Fast sampling devices for dynamic exhaust gas analysis (1991)

Grisar, R., Riedel, W., Wolf, H., Wißler, R.

We report on a fast exhaust gas sampling system, optimized for infrared diode laser gas analysis, but also applicable to other techniques. A system response time of 3 ms and a gas delay time of appr....

Dynamische Abgasmessung mit Diodenlasern. (1991)

Nitzschke, E., Wolf, H.

We report on a fast exhaust gas analysis system, based on infrared spectroscopy of a single absorption line by pulse-operated diode lasers and a newly designed gas sampling system. Otto and Diesel...

Development and evaluation of biosensors for HIV-serology (1991)

Aberl, F., Wolf, H., Woias, P., Koch, S., Kößlinger, C., Drost, S.

During the last decade a new generation of sensors, the biosensors, has been developed for the quantitative measurement of biological substances. In serological diagnosis the rapid and sensitive...

Photoemission study on the valence bands of DMe-DCNQI derived charge-transfer salts (1991)

Schmeißer, D., Gonzales, A., Von Schütz, J., Wachtel, H., Wolf, H.

The electronic structure of crystalline needles of the 2,5-Dimethyl substituted (DCNQI)2M (M = Cu, Rb, Cs) radical-anion salts, prepared in situ, is determined by photoelectrons spectroscopy using...

Photoemission study on the valence bands of DMe-DCNQI derived charge-transfer salts (1991)

Schmeißer, D., Gonzales, A., Von Schütz, J., Wachtel, H., Wolf, H.

The electronic structure of crystalline needles of the 2,5-Dimethyl substituted (DCNQI)2M (M = Cu, Rb, Cs) radical-anion salts, prepared in situ, is determined by photoelectrons spectroscopy using...

Verfahren und Vorrichtung zur quantitativen Bestimmung optisch aktiver Substanzen (1990)

Riedel, W., Schmidtke, G., Wolf, H.

Eine Vorrichtung zur quantitativen Bestimmung optisch aktiver Substanzen verfuegt ueber zwei Lumineszenzdioden (1, 21) mit jeweils einer Kollimatorlinse (3, 23) und einem Polarisationsfilter (5, 25),...

A quartz crystal biosensor for measurment in liquids (1990)

Kößlinger, C., Drost, S., Aberl, F., Wolf, H., Koch, S., Woias, P.

The detection of anti-HIV antibodies by means of synthetic HIV-peptide immobilized on a piezoelectric quartz sensor is demonstrated. The measurement set-up consists of an oscillator circuit, a...

Verfahren und Vorrichtung zur Gasanalyse (1989)

Klocke, U., Schmidtke, G., Wolf, H.

Ein Gasanalysegeraet verfuegt ueber einen Messzweig (37) mit einer Auswerteschaltung (35) mit Fourierfilterung sowie einen Referenzzweig (12), der ueber eine Steuerungsschaltung (22) zur Ablenkung...

Laserabsorptionsspektrometer (1989)

Grisar, R., Riedel, W., Halford, B., Wolf, H., Klocke, U., Preier, H.M.

Ein Laserabsorptionsspektrometer verfuegt ueber einen Regelkreis zur Frequenzstabilisierung eines pulsbetriebenen Diodenlasers (1). Ein Teil der Laserstrahlung (5) wird als Referenzstrahl (8) durch...

Infrarot-Analysengeraet (1989)

Schmidtke, G., Wolf, H., Fischer, W.

Ein Infrarot-Analysengeraet, das zum Bestimmen der Konzentration eines Gases dient, verfuegt ueber eine breitbandige Strahlungsquelle (1), deren Licht von zwei Konkavspiegeln (13, 18) derart...

Dynamic car exhaust gas analysis using tunable IR diode lasers (1989)

Grisar, R., Klocke, U., Riedel, W.J., Wolf, H., Wißler, R.

We report on the dynamic car exhaust gas analysis with single ignition cycle resolution. The optical setup using pulsed integrative IR diode laser spectroscopy as well as the fast sampling system are...

Diode laser spectrometer for monitoring up to five atmospheric trace gases in unattended operation. (1989)

Klocke, U., Knothe, M., Kohn, W., Riedel, W.J., Schmidtke, G., Wolf, H.

An automated diode laser spectrometer for recording concentrations of trace gases is described. It has been tested for unattended operation in the laboratory and at an atmospheric surveillance...

Atmospheric trace gas measurement on the Schauinsland surveying station (1989)

Klocke, U., Knothe, M., Kohn, W., Riedel, W.J., Schmidtke, G., Wolf, H.

An automated diode laser spectrometer (DLS) as described has been tested in unattended operation on the Schauinsland surveying station. After selecting diode lasers with uniform far-field pattern and...

Quantitative Analyse von atmosphärischen Spurengasen. (1988)

Klocke, U., Knothe, M., Kohn, W., Riedel, W.J., Schmidtke, G., Wolf, H.

Im Rahmen dieses Vorhabens wurde das erste vollautomatisch arbeitende Diodenlaser-Spektrometer entwickelt und erprobt, das bis zu fuenf Gase im ppb- und sub-ppb-Bereich misst. Von besonderem...

Time-resolved car-exhaust gas analysis using tunable IR diode lasers. (1988)

Grisar, R., Riedel, W.J., Wolf, H., Wißler, R.

We report first results of car exhaust gas monitoring with time resolution in the millisecond range using tunable lead salt diode lasers and a fast heating gas sampling system with a low pressure...

The antigenic index: a novel algorithm for predicting antigenic determinants (1988)

Jameson, B.A., Wolf, H.

In this paper, we introduce a computer algorithm which can be used to predict the topological features of a protein directly from its primary amino acid sequence. The computer program generates...

An integrated family of amino acid sequence analysis programs (1988)

Wolf, H., Modrow, S., Motz, M., Jameson, B.A., Hermann, G., Förtsch, B.

During the last years abundant sequence data has become available due to the rapid progress in protein and DNA sequencing techniques. The exact three-dimensional structures, however, are available...

Spektrometer mit einem Infrarot-Diodenlaser (1987)

Riedel, W., Wolf, H., Grisar, R., Schmidtke, G., Pfirrmann, A., Preier, H.M.

Ein Spektrometer mit einem Infrarot-Diodenlaser (1), einem Monochromator und einem Detektor verfuegt ueber einen beugungsbegrenzten Kollimator mit einer niedrig brechenden Linse (2) in Lupenanordnung...

Diodenlaser-Prozeßspektrometrie zur Rauchgasanalyse in Kohlekraftwerken (1987)

Grisar, R., Klocke, U., Knothe, M., Riedel, W., Wolf, H., Wißler, R.

Es wird ein als Prototyp entwickeltes Zwei-Komponenten-Rauchgasanalysegeraet beschrieben, das auf der Spektrometrie mit abstimmbaren Bleisalz-Diodenlasern beruht. Der optische und elektronische...

Vorrichtung zum Kuehlen optoelektronischer Bauelemente (1986)

Riedel, W., Wissler, R., Wolf, H., Knothe, M., Grisar, R., Hoffmann, R.

Ein vibrationsentkoppelter Kuehler fuer Infrarot-Diodenlaser und Infrarot-Detektoren (9) verfuegt ueber eine Halterung (8), die an einem Stab (12) befestigt ist, der zusammen mit zwei Stuetzen (16,...

Quantitative Analyse von atmosphaerischen Spurengasen (1986)

Riedel, W.J., Wolf, H., Klocke, U., Knothe, M., Kohn, W., Schmidtke, G.

Es wird ein IR-Laserdioden-Spektrometer entwickelt, mit dem die am Ozonkreislauf beteiligten Spurengasse NO, NO2, HNO3 und Ozon sowie zusaetzlich SO2 gemessen werden sollen. Das Geraet wird in einem...

NH3-measurements in power plants with "DENOX" installations (1986)

Wolf, H., Riedel, W.J.

Mit Hilfe eines Diodenlaserspektrometers wurde der NH3-Schlupf im Abgas eines "Denox"-Katalysators in einem Kohlekraftwerk gemessen. Diese Messungen wurden mit einem "line locking"- System und nach...

Automated diode laser spectrometer for monitoring five atmosphere trace gases (1986)

Riedel, W.J., Wolf, H., Klocke, U., Knothe, M., Kohn, W., Schmidtke, G.

Ein automatisiertes Diodenlaserspektrometer zur Bestimmung der Konzentrationen der atmosphaerischen Spurengase NO, NO2, HNO3, O3 und SO3 befinden sich in der Entwicklung. Der experimentelle Aufbau...

TIME-RESOLVED EXCIMER FORMATION IN PYRENE CRYSTALS (1985)

Port, H., Seyfang, R., Wolf, H.

Fluorescence transients of pyrene single crystals are measured with picosecond time resolution (4 K ÷ 300 K). The results indicate a different excimer formation process in low and high temperature...

Mehrkanal-Prozess-Spektrometer (1985)

Klocke, U., Knothe, M., Riedel, W., Schmidtke, G., Wolf, H., Preier, H.M.

Bei einem Mehrkanal-Prozess-Spektrometer wird durch Verstellen eines dispergierenden und reflektierenden Elementes eine Verstimmung des auszuwertenden Spektralbereichs durchgefuehrt. Die dem...

TIME-RESOLVED EXCIMER FORMATION IN PYRENE CRYSTALS (1985)

Port, H., Seyfang, R., Wolf, H.

Fluorescence transients of pyrene single crystals are measured with picosecond time resolution (4 K ÷ 300 K). The results indicate a different excimer formation process in low and high temperature...

TIME-RESOLVED EXCIMER FORMATION IN PYRENE CRYSTALS (1985)

Port, H., Seyfang, R., Wolf, H.

Fluorescence transients of pyrene single crystals are measured with picosecond time resolution (4 K ÷ 300 K). The results indicate a different excimer formation process in low and high temperature...

Select Minerals and Potable Use of Reclaimed Wastewaters (1977)

Wolf, H.

The long-observed relationships of an influence of drinking water mineral content on heart-circulatory deaths are developed to indicate that sodium -- when present in sufficiently high concentrations...

Select Minerals and Potable Use of Reclaimed Wastewaters (1977)

Wolf, H.

The long-observed relationships of an influence of drinking water mineral content on heart-circulatory deaths are developed to indicate that sodium -- when present in sufficiently high concentrations...

Über Einige Wegwespen (Hymenoptera, Pompilidae) aus dem Rijksmuseum van Natuurlijke Historie zu Leiden (1975)

Wolf, H.

Aus dem Reichsmuseum zu Leiden (Niederlande) lagen mir 1972 und 1973 zur Bestimmung 236 westpaläarktische Wegwespen-Arten und -Unterarten in 4131 Exemplaren vor, und zwar 2749 ♀ und 1382 ♂. Sie...

SPECTROSCOPIE ÉLECTRONIQUE DES MOLÉCULES POLYATOMIQUES ET DES SOLIDES ORGANIQUESEXCITONS, AGGREGATES AND DEFECTS IN ORGANIC SOLIDS (1971)

Wolf, H.

Spectroscopic studies using optical and ESR spectroscopy on organic molecular crystals like naphthalene and anthracene are discussed, which give the following information : the magnitude of the...

SPECTROSCOPIE ÉLECTRONIQUE DES MOLÉCULES POLYATOMIQUES ET DES SOLIDES ORGANIQUESEXCITONS, AGGREGATES AND DEFECTS IN ORGANIC SOLIDS (1971)

Wolf, H.

Spectroscopic studies using optical and ESR spectroscopy on organic molecular crystals like naphthalene and anthracene are discussed, which give the following information : the magnitude of the...

SPECTROSCOPIE ÉLECTRONIQUE DES MOLÉCULES POLYATOMIQUES ET DES SOLIDES ORGANIQUESEXCITONS, AGGREGATES AND DEFECTS IN ORGANIC SOLIDS (1971)

Wolf, H.

Spectroscopic studies using optical and ESR spectroscopy on organic molecular crystals like naphthalene and anthracene are discussed, which give the following information : the magnitude of the...

Expression of proteins encoded by Epstein-Barr virus trans-activator genes depends on the differentiation of epithelial cells in oral hairy leukoplakia.

Becker, J, Leser, U, Marschall, M, Langford, A, Jilg, W, Gelderblom, H, ...

The Epstein-Barr virus (EBV) immediate early gene product BZLF1 was localized by indirect immunofluorescence to the cytoplasm of the basal epithelial layer at the lateral border and dorsum of tongue...

Epstein-Barr virus lytic replication is controlled by posttranscriptional negative regulation of BZLF1.

Prang, N, Wolf, H, Schwarzmann, F

Regulation of the immediate-early gene BZLF1 is assumed to play a key role in triggering the lytic replication of Epstein-Barr virus (EBV). The expression of BZLF1 is regulated on multiple levels,...

Specific cytotoxic T lymphocytes recognize the immediate-early transactivator Zta of Epstein-Barr virus.

Bogedain, C, Wolf, H, Modrow, S, Stuber, G, Jilg, W

We identified the immediate-early transactivator Zta of Epstein-Barr virus as a target for specific cytotoxic T lymphocytes (CTL). Cells pulsed with overlapping synthetic peptides representing the...

Lymphoproliferative responses after infection with human parvovirus B19.

Von Poblotzki, A, Gerdes, C, Reischl, U, Wolf, H, Modrow, S

Immunity after infection with the parvovirus B19 is assumed to be conferred by a humoral immune response with development of neutralizing antibody. In contrast, little is known about the nature of...

Structural analysis of the adenovirus type 5 E1B 55-kilodalton-E4orf6 protein complex.

Rubenwolf, S, Schütt, H, Nevels, M, Wolf, H, Dobner, T

The adenovirus type 5 (Ad5) early 1B (E1B) 55-kDa (E1B-55kDa)-E4orf6 protein complex has been implicated in the selective modulation of nucleocytoplasmic mRNA transport at late times after infection....

Unique antibiotic sensitivity of archaebacterial polypeptide elongation factors.

Londei, P, Sanz, J L, Altamura, S, Hummel, H, Cammarano, P, Amils, R, ...

The antibiotic sensitivity of the archaebacterial factors catalyzing the binding of aminoacyl-tRNA to ribosomes (elongation factor Tu [EF-Tu] for eubacteria and elongation factor 1 [EF1] for...

Different activation of Epstein-Barr virus immediate-early and early genes in Burkitt lymphoma cells and lymphoblastoid cell lines.

Bogedain, C, Alliger, P, Schwarzmann, F, Marschall, M, Wolf, H, Jilg, W

Specific expression of the Epstein-Barr virus (EBV) immediate-early and early gene products Zta, Rta, I'ta, and MSta by a recombinant vaccinia virus system allowed us to analyze the first steps in...

Identification of proteins encoded by Epstein-Barr virus trans-activator genes.

Marschall, M, Leser, U, Seibl, R, Wolf, H

Specific antisera were generated to characterize Epstein-Barr virus proteins reported to have trans-activating properties. Open reading frame BRLF1 was found to be expressed in two modifications in...

Synthetic peptides define the fine specificity of the human immunodeficiency virus (HIV) gp160 humoral immune response in HIV type 1-infected chimpanzees.

Warren, R Q, Wolf, H, Shuler, K R, Eichberg, J W, Zajac, R A, Boswell, R N, ...

The fine specificities of antibodies produced against human immunodeficiency virus type 1 (HIV-1) gp160 were examined in sera from 23 HIV-1-infected chimpanzees. These animals had been infected with...

Mapping of herpesvirus saimiri proteins on the viral genome: proteins dependent and not dependent on viral DNA synthesis.

Hell, W, Modrow, S, Wolf, H

Hybrid selected translation was used to map the genome of herpesvirus saimiri, a lymphotropic and oncogenic herpesvirus. RNA extracted from virus-infected cells was hybridized to cloned genomic...

Detection of pseudorabies virus DNA in the inner ear of intranasally infected BALB/c mice with nucleic acid hybridization in situ.

Falser, N, Bandtlow, I, Haus, M, Wolf, H

Evidence for the pathogenicity of pseudorabies virus for the auditory and vestibular organs of experimentally infected mice is presented. We demonstrate viral genomes in cells of the peripheral...

Strain-specific transcription and translation of the BamHI Z area of Epstein-Barr Virus.

Seibl, R, Motz, M, Wolf, H

The expression of the 1,800-base-pair BamHI Z region of Epstein-Barr virus DNA was analyzed by hybrid-selected translation with several DNA subclones and RNA from different cell lines. Furthermore,...

Computer-assisted analysis of envelope protein sequences of seven human immunodeficiency virus isolates: prediction of antigenic epitopes in conserved and variable regions.

Modrow, S, Hahn, B H, Shaw, G M, Gallo, R C, Wong-Staal, F, Wolf, H

Independent isolates of human immunodeficiency virus (HIV) exhibit a striking genomic diversity, most of which is located in the viral envelope gene. Since this property of the HIV group of viruses...

Persistence of Epstein-Barr virus in the parotid gland.

Wolf, H, Haus, M, Wilmes, E

Two independent techniques, in situ hybridization on frozen sections and reassociation kinetics, have been used to localize Epstein-Barr virus genomes in tissue samples from healthy human adults....

Polymorphic proteins encoded within BZLF1 of defective and standard Epstein-Barr viruses disrupt latency.

Countryman, J, Jenson, H, Seibl, R, Wolf, H, Miller, G

These experiments identify an Epstein-Barr virus-encoded gene product, called ZEBRA (BamHI fragment Z Epstein-Barr replication activator) protein, which activates a switch between the latent and...

Effect of the arginine analog canavanine on the synthesis of Epstein-Barr virus-induced proteins in superinfected Raji cells.

Bayliss, G J, Wolf, H

The addition of canavanine to cultures of superinfected Raji cells in the absence of arginine prevented the appearance of early antigens as defined by immunofluorescent staining. Addition of various...

An orally administered bacterial immunomodulator primes rabbit neutrophils for increased oxidative burst in response to opsonized zymosan.

Helmberg, A, Böck, G, Wolf, H, Wick, G

To assess the potential effect of an orally administered immunomodulator, consisting of a lysate of seven different bacteria, on polymorphonuclear leukocyte (PMN) function, rabbits were fed this...

Comparison of antibody reactivity to human immunodeficiency virus type 1 (HIV-1) gp160 epitopes in sera from HIV-1-infected individuals from Tanzania and from the United States.

Warren, R Q, Nkya, W M, Shao, J F, Anderson, S A, Wolf, H, Hendrix, C W, ...

In this study, we compared sera from 159 human immunodeficiency virus type 1 (HIV-1)-infected individuals from Tanzania and 103 infected individuals from the United States for antibodies reactive...

Serodiagnosis of infectious mononucleosis by using recombinant Epstein-Barr virus antigens and enzyme-linked immunosorbent assay technology.

Gorgievski-Hrisoho, M, Hinderer, W, Nebel-Schickel, H, Horn, J, Vornhagen, R, Sonneborn, H H, ...

Four recombinant, diagnostically useful Epstein-Barr virus (EBV) proteins representative of the viral capsid antigen (p150), diffuse early antigen (p54), the major DNA-binding protein (p138), and the...

New oligopeptide immunoglobulin G test for human parvovirus B19 antibodies.

Schwarz, T F, Modrow, S, Hottenträger, B, Höflacher, B, Jäger, G, Scharti, W, ...

A new, highly sensitive and specific enzyme immunoassay using oligopeptides as antigen (enzyme-linked immunosorbent assay [ELISA] B19-OP) for detecting parvovirus B19-specific immunoglobulin G (IgG)...

Immunoglobulin M anti-hepatitis A virus determination reorienting gradient centrifugation for diagnosis of Acute Hepatitis A.

Frösner, G G, Scheid, R, Wolf, H, Deinhardt, F

The persistence of antibody to hepatitis A antigen (anti-HAV) of the immunoglobulin M (IgM) class was evaluated in 88 sera of 51 acute hepatitis A patients. IgM was separated from IgG by a 2-h...

Expression of Epstein-Barr virus genes in different cell types after microinjection of viral DNA.

Graessmann, A, Wolf, H, Bornkamm, G W

Gene expression of Epstein-Barr virus (EBV) was studied after microinjection of viral DNA into different types of cells. Raji TK- cells, known to express viral gene functions after superinfection...

An Epstein--Barr virus early protein induces cell fusion.

Bayliss, G J, Wolf, H

Superinfection of Raji cells with Epstein--Barr virus (EBV) leads to syncytium formation. Studies using metabolic inhibitors and amino acid analogues suggest that the fusion-inducing factor belongs...

Purification of myosin light chain kinase from bovine cardiac muscle.

Wolf, H, Hofmann, F

Myosin light chain kinase was purified > 100,000-fold to apparent homogeneity with a yield of 10% from bovine cardiac muscle. Sodium dodecyl sulfate gels of the purified kinase showed one stained...

Immune response in the lungs following oral immunization with bacterial lysates of respiratory pathogens.

Ruedl, C, Frühwirth, M, Wick, G, Wolf, H

We have investigated the local immune response of the BALB/c mouse respiratory tract after oral immunization with a bacterial lysate of seven common respiratory pathogens. After two immunization on...

Characterization of two related Epstein-Barr virus-encoded membrane proteins that are differentially expressed in Burkitt lymphoma and in vitro-transformed cell lines.

Modrow, S, Wolf, H

Two related but differentially expressed potential membrane proteins of Epstein-Barr virus are encoded by the same reading frame in the EcoRI D het region of the viral genome. Potential antigenic...

Pulvomycin, an inhibitor of protein biosynthesis preventing ternary complex formation between elongation factor Tu, GTP, and aminoacyl-tRNA.

Wolf, H, Assmann, D, Fischer, E

Pulvomycin and the synonymous antibiotics labilomycin and 1063-Z are shown to inhibit prokaryotic protein synthesis by acting on elongation factor Tu (EF-Tu): in the presence of the antibiotic, the...

Lipid utilization by human lymphocytes is correlated with high-density-lipoprotein binding site activity.

Xu, Q, Jürgens, G, Huber, L A, Böck, G, Wolf, H, Wick, G

The nature and physiological importance of high-density lipoprotein (HDL) binding sites on unstimulated (resting) and mitogen-activated (blast) human peripheral blood lymphocytes were investigated....

Heterogeneous nuclear ribonucleoprotein E1B-AP5 is methylated in its Arg-Gly-Gly (RGG) box and interacts with human arginine methyltransferase HRMT1L1.

Kzhyshkowska, J, Schütt, H, Liss, M, Kremmer, E, Stauber, R, Wolf, H, ...

The heterogeneous nuclear ribonucleoprotein (hnRNP) family includes predominantly nuclear proteins acting at different stages of mRNA metabolism. A characteristic feature of hnRNPs is to undergo...

Control by pulse parameters of electric field-mediated gene transfer in mammalian cells.

Wolf, H, Rols, M P, Boldt, E, Neumann, E, Teissié, J

Electric field-mediated gene transfer in mammalian cells (electrotransformation) depends on the pulsing conditions (field intensity, pulse duration, number of pulses). The effect of these parameters...

Red blood cells experience electrostatic repulsion but make molecular adhesions with glass.

Trommler, A, Gingell, D, Wolf, H

We have studied the detachment of unfixed red cells from glass coverslips under unit gravity and by centrifugation in buffered isotonic solutions over a range of ionic strengths. Cell-glass contact...

Chorionic villi sampling: cytogenetic and clinical findings in 500 pregnancies.

Leschot, N J, Wolf, H, Verjaal, M, De Boer, E G, Kanhai, H H, ...

The cytogenetic findings were analysed in a series of 500 pregnancies in which chorionic villi sampling was performed. In all cases a direct method was used, karyotyping being successful in 481 cases...

N-Acetyltransferase phenotype and risk in urinary bladder cancer: approaches in molecular epidemiology. Preliminary results in Sweden and Denmark

Lower, G. M., Nilsson, T., Nelson, C. E., Wolf, H., Gamsky, T. E., Bryan, G. T.

A variable but often significant proportion of urinary bladder cancer in urban areas can be attributed to occupational and cultural (cigarette smoking) situations associated with exposures to various...

Expression of proteins encoded by Epstein-Barr virus trans-activator genes depends on the differentiation of epithelial cells in oral hairy leukoplakia.

Becker, J, Leser, U, Marschall, M, Langford, A, Jilg, W, Gelderblom, H, ...

The Epstein-Barr virus (EBV) immediate early gene product BZLF1 was localized by indirect immunofluorescence to the cytoplasm of the basal epithelial layer at the lateral border and dorsum of tongue...

Epstein-Barr virus lytic replication is controlled by posttranscriptional negative regulation of BZLF1.

Prang, N, Wolf, H, Schwarzmann, F

Regulation of the immediate-early gene BZLF1 is assumed to play a key role in triggering the lytic replication of Epstein-Barr virus (EBV). The expression of BZLF1 is regulated on multiple levels,...

Specific cytotoxic T lymphocytes recognize the immediate-early transactivator Zta of Epstein-Barr virus.

Bogedain, C, Wolf, H, Modrow, S, Stuber, G, Jilg, W

We identified the immediate-early transactivator Zta of Epstein-Barr virus as a target for specific cytotoxic T lymphocytes (CTL). Cells pulsed with overlapping synthetic peptides representing the...

Lymphoproliferative responses after infection with human parvovirus B19.

Von Poblotzki, A, Gerdes, C, Reischl, U, Wolf, H, Modrow, S

Immunity after infection with the parvovirus B19 is assumed to be conferred by a humoral immune response with development of neutralizing antibody. In contrast, little is known about the nature of...

Structural analysis of the adenovirus type 5 E1B 55-kilodalton-E4orf6 protein complex.

Rubenwolf, S, Schütt, H, Nevels, M, Wolf, H, Dobner, T

The adenovirus type 5 (Ad5) early 1B (E1B) 55-kDa (E1B-55kDa)-E4orf6 protein complex has been implicated in the selective modulation of nucleocytoplasmic mRNA transport at late times after infection....

Unique antibiotic sensitivity of archaebacterial polypeptide elongation factors.

Londei, P, Sanz, J L, Altamura, S, Hummel, H, Cammarano, P, Amils, R, ...

The antibiotic sensitivity of the archaebacterial factors catalyzing the binding of aminoacyl-tRNA to ribosomes (elongation factor Tu [EF-Tu] for eubacteria and elongation factor 1 [EF1] for...

Different activation of Epstein-Barr virus immediate-early and early genes in Burkitt lymphoma cells and lymphoblastoid cell lines.

Bogedain, C, Alliger, P, Schwarzmann, F, Marschall, M, Wolf, H, Jilg, W

Specific expression of the Epstein-Barr virus (EBV) immediate-early and early gene products Zta, Rta, I'ta, and MSta by a recombinant vaccinia virus system allowed us to analyze the first steps in...

Identification of proteins encoded by Epstein-Barr virus trans-activator genes.

Marschall, M, Leser, U, Seibl, R, Wolf, H

Specific antisera were generated to characterize Epstein-Barr virus proteins reported to have trans-activating properties. Open reading frame BRLF1 was found to be expressed in two modifications in...

Synthetic peptides define the fine specificity of the human immunodeficiency virus (HIV) gp160 humoral immune response in HIV type 1-infected chimpanzees.

Warren, R Q, Wolf, H, Shuler, K R, Eichberg, J W, Zajac, R A, Boswell, R N, ...

The fine specificities of antibodies produced against human immunodeficiency virus type 1 (HIV-1) gp160 were examined in sera from 23 HIV-1-infected chimpanzees. These animals had been infected with...

Mapping of herpesvirus saimiri proteins on the viral genome: proteins dependent and not dependent on viral DNA synthesis.

Hell, W, Modrow, S, Wolf, H

Hybrid selected translation was used to map the genome of herpesvirus saimiri, a lymphotropic and oncogenic herpesvirus. RNA extracted from virus-infected cells was hybridized to cloned genomic...

Detection of pseudorabies virus DNA in the inner ear of intranasally infected BALB/c mice with nucleic acid hybridization in situ.

Falser, N, Bandtlow, I, Haus, M, Wolf, H

Evidence for the pathogenicity of pseudorabies virus for the auditory and vestibular organs of experimentally infected mice is presented. We demonstrate viral genomes in cells of the peripheral...

Strain-specific transcription and translation of the BamHI Z area of Epstein-Barr Virus.

Seibl, R, Motz, M, Wolf, H

The expression of the 1,800-base-pair BamHI Z region of Epstein-Barr virus DNA was analyzed by hybrid-selected translation with several DNA subclones and RNA from different cell lines. Furthermore,...

Computer-assisted analysis of envelope protein sequences of seven human immunodeficiency virus isolates: prediction of antigenic epitopes in conserved and variable regions.

Modrow, S, Hahn, B H, Shaw, G M, Gallo, R C, Wong-Staal, F, Wolf, H

Independent isolates of human immunodeficiency virus (HIV) exhibit a striking genomic diversity, most of which is located in the viral envelope gene. Since this property of the HIV group of viruses...

Persistence of Epstein-Barr virus in the parotid gland.

Wolf, H, Haus, M, Wilmes, E

Two independent techniques, in situ hybridization on frozen sections and reassociation kinetics, have been used to localize Epstein-Barr virus genomes in tissue samples from healthy human adults....

Polymorphic proteins encoded within BZLF1 of defective and standard Epstein-Barr viruses disrupt latency.

Countryman, J, Jenson, H, Seibl, R, Wolf, H, Miller, G

These experiments identify an Epstein-Barr virus-encoded gene product, called ZEBRA (BamHI fragment Z Epstein-Barr replication activator) protein, which activates a switch between the latent and...

Effect of the arginine analog canavanine on the synthesis of Epstein-Barr virus-induced proteins in superinfected Raji cells.

Bayliss, G J, Wolf, H

The addition of canavanine to cultures of superinfected Raji cells in the absence of arginine prevented the appearance of early antigens as defined by immunofluorescent staining. Addition of various...

An orally administered bacterial immunomodulator primes rabbit neutrophils for increased oxidative burst in response to opsonized zymosan.

Helmberg, A, Böck, G, Wolf, H, Wick, G

To assess the potential effect of an orally administered immunomodulator, consisting of a lysate of seven different bacteria, on polymorphonuclear leukocyte (PMN) function, rabbits were fed this...

Comparison of antibody reactivity to human immunodeficiency virus type 1 (HIV-1) gp160 epitopes in sera from HIV-1-infected individuals from Tanzania and from the United States.

Warren, R Q, Nkya, W M, Shao, J F, Anderson, S A, Wolf, H, Hendrix, C W, ...

In this study, we compared sera from 159 human immunodeficiency virus type 1 (HIV-1)-infected individuals from Tanzania and 103 infected individuals from the United States for antibodies reactive...

Serodiagnosis of infectious mononucleosis by using recombinant Epstein-Barr virus antigens and enzyme-linked immunosorbent assay technology.

Gorgievski-Hrisoho, M, Hinderer, W, Nebel-Schickel, H, Horn, J, Vornhagen, R, Sonneborn, H H, ...

Four recombinant, diagnostically useful Epstein-Barr virus (EBV) proteins representative of the viral capsid antigen (p150), diffuse early antigen (p54), the major DNA-binding protein (p138), and the...

New oligopeptide immunoglobulin G test for human parvovirus B19 antibodies.

Schwarz, T F, Modrow, S, Hottenträger, B, Höflacher, B, Jäger, G, Scharti, W, ...

A new, highly sensitive and specific enzyme immunoassay using oligopeptides as antigen (enzyme-linked immunosorbent assay [ELISA] B19-OP) for detecting parvovirus B19-specific immunoglobulin G (IgG)...

Immunoglobulin M anti-hepatitis A virus determination reorienting gradient centrifugation for diagnosis of Acute Hepatitis A.

Frösner, G G, Scheid, R, Wolf, H, Deinhardt, F

The persistence of antibody to hepatitis A antigen (anti-HAV) of the immunoglobulin M (IgM) class was evaluated in 88 sera of 51 acute hepatitis A patients. IgM was separated from IgG by a 2-h...

Expression of Epstein-Barr virus genes in different cell types after microinjection of viral DNA.

Graessmann, A, Wolf, H, Bornkamm, G W

Gene expression of Epstein-Barr virus (EBV) was studied after microinjection of viral DNA into different types of cells. Raji TK- cells, known to express viral gene functions after superinfection...

An Epstein--Barr virus early protein induces cell fusion.

Bayliss, G J, Wolf, H

Superinfection of Raji cells with Epstein--Barr virus (EBV) leads to syncytium formation. Studies using metabolic inhibitors and amino acid analogues suggest that the fusion-inducing factor belongs...

Purification of myosin light chain kinase from bovine cardiac muscle.

Wolf, H, Hofmann, F

Myosin light chain kinase was purified > 100,000-fold to apparent homogeneity with a yield of 10% from bovine cardiac muscle. Sodium dodecyl sulfate gels of the purified kinase showed one stained...

Immune response in the lungs following oral immunization with bacterial lysates of respiratory pathogens.

Ruedl, C, Frühwirth, M, Wick, G, Wolf, H

We have investigated the local immune response of the BALB/c mouse respiratory tract after oral immunization with a bacterial lysate of seven common respiratory pathogens. After two immunization on...

Characterization of two related Epstein-Barr virus-encoded membrane proteins that are differentially expressed in Burkitt lymphoma and in vitro-transformed cell lines.

Modrow, S, Wolf, H

Two related but differentially expressed potential membrane proteins of Epstein-Barr virus are encoded by the same reading frame in the EcoRI D het region of the viral genome. Potential antigenic...

Pulvomycin, an inhibitor of protein biosynthesis preventing ternary complex formation between elongation factor Tu, GTP, and aminoacyl-tRNA.

Wolf, H, Assmann, D, Fischer, E

Pulvomycin and the synonymous antibiotics labilomycin and 1063-Z are shown to inhibit prokaryotic protein synthesis by acting on elongation factor Tu (EF-Tu): in the presence of the antibiotic, the...

Lipid utilization by human lymphocytes is correlated with high-density-lipoprotein binding site activity.

Xu, Q, Jürgens, G, Huber, L A, Böck, G, Wolf, H, Wick, G

The nature and physiological importance of high-density lipoprotein (HDL) binding sites on unstimulated (resting) and mitogen-activated (blast) human peripheral blood lymphocytes were investigated....

Heterogeneous nuclear ribonucleoprotein E1B-AP5 is methylated in its Arg-Gly-Gly (RGG) box and interacts with human arginine methyltransferase HRMT1L1.

Kzhyshkowska, J, Schütt, H, Liss, M, Kremmer, E, Stauber, R, Wolf, H, ...

The heterogeneous nuclear ribonucleoprotein (hnRNP) family includes predominantly nuclear proteins acting at different stages of mRNA metabolism. A characteristic feature of hnRNPs is to undergo...

Chorionic villi sampling: cytogenetic and clinical findings in 500 pregnancies.

Leschot, N J, Wolf, H, Verjaal, M, De Boer, E G, Kanhai, H H, ...

The cytogenetic findings were analysed in a series of 500 pregnancies in which chorionic villi sampling was performed. In all cases a direct method was used, karyotyping being successful in 481 cases...

Control by pulse parameters of electric field-mediated gene transfer in mammalian cells.

Wolf, H, Rols, M P, Boldt, E, Neumann, E, Teissié, J

Electric field-mediated gene transfer in mammalian cells (electrotransformation) depends on the pulsing conditions (field intensity, pulse duration, number of pulses). The effect of these parameters...

Red blood cells experience electrostatic repulsion but make molecular adhesions with glass.

Trommler, A, Gingell, D, Wolf, H

We have studied the detachment of unfixed red cells from glass coverslips under unit gravity and by centrifugation in buffered isotonic solutions over a range of ionic strengths. Cell-glass contact...

N-Acetyltransferase phenotype and risk in urinary bladder cancer: approaches in molecular epidemiology. Preliminary results in Sweden and Denmark

Lower, G. M., Nilsson, T., Nelson, C. E., Wolf, H., Gamsky, T. E., Bryan, G. T.

A variable but often significant proportion of urinary bladder cancer in urban areas can be attributed to occupational and cultural (cigarette smoking) situations associated with exposures to various...

Liberal diagnosis and treatment of intrauterine infection reduces early-onset neonatal group B streptococcal infection but not sepsis by other pathogens.

Wolf, H, Schaap, A H, Smit, B J, Spanjaard, L, Adriaanse, A H

OBJECTIVE: Comparison of the incidence and case fatality of early-onset group B streptococcus sepsis and sepsis caused by other pathogens in neonates after change of management of intrauterine...

Ankylosing hyperostosis: a study of HLA A, B, and C antigens.

Perry, J D, Wolf, H, Festenstein, H, Storey, G O

HLA A, B, and C antigens were studied in 25 patients with ankylosing hyperostosis. There was no evidence of an increased frequency of any antigen in association with the condition itself. However, a...

Subcellular distribution and life cycle of Epstein-Barr virus in keratinocytes of oral hairy leukoplakia.

Rabanus, J. P., Greenspan, D., Petersen, V., Leser, U., Wolf, H., Greenspan, J. S.

The authors investigated the life cycle of Epstein-Barr virus (EBV) in keratinocytes of oral hairy leukoplakia by combining immunohistochemistry. DNA in situ hybridization, and lectin histochemistry...

Characterization of antisera against bovine prolactin for in vivo studies on prolactin function in the rat.

Kofler, R, Tabarelli, M, Schwarz, S, Wolf, H, Loewit, K, Wick, G

The IgG fraction of rabbit antisera to bovine prolactin (PRL), intended for in vivo studies on the role of PRL in the rat, was prepared and characterized in vitro and in vivo. The antibodies showed a...

Chemokines in onchocerciasis patients after a single dose of ivermectin

Fendt, J, Hamm, DM, Banla, M, Schulz-Key, H, Wolf, H, Helling-Giese, G, ...

Ivermectin treatment will effectively diminish microfilariae (Mf) of Onchocerca volvulus in the skin of patients, but therapy is associated with adverse host inflammatory responses. To investigate...

Influence of obstetric management on outcome of extremely preterm growth retarded infants

Schaap, A, Wolf, H, Bruinse, H, Den Ouden, A L, Haas, H, Van Ertbruggen, I, ...

AIM—To describe the long term outcome of extremely preterm growth retarded infants in relation to obstetric management and various perinatal events.
METHODS—A cohort study was undertaken in two...

Induction of abortive and productive proliferation in resting human T lymphocytes via CD3 and CD28

Müller, Y, Wolf, H, Wierenga, E, Jung, G

How the T-cell receptor (TCR)/CD3 complex mediates positive as well as negative signals for T-cell regulation is not fully understood. We have previously described the induction of anergy in resting...

Regulation of cytochrome P450 gene expression in human colon and breast tumour xenografts.

Smith, G., Harrison, D. J., East, N., Rae, F., Wolf, H., Wolf, C. R.

It is extremely difficult to identify the factors which regulate the expression of drug-metabolising enzymes in man. To address this problem, we have developed a model involving the use of human...

Frequency and mechanism of Lewis antigen expression in human urinary bladder and colon carcinoma patients.

Langkilde, N. C., Wolf, H., Meldgård, P., Orntoft, T. F.

Changes in the expression of Lewis antigens have been associated with cancer diseases, and recent results have pointed at a possible increased risk of cancer development among Lewis negative...

Extracorporeal immunoadsorption of circulating specific serum factors in cancer patients.

Langvad, E., Hydén, H., Wolf, H., Kroeigaard, N.

Circulating serum factors have been said to abrogate the effects of immune response in cancer, i.e. "blocking" and "antigenic inhibition". The aim of this investigation was to isolate such specific...