HA Chase

Production of enzymatically active ketosteroid isomerase following insoluble expression in Escherichia coli (2006)

Hutchinson, MH, Morrealle, G, Middelberg, APJ, Chase, HA

Enzymatically active Delta(5)-3-ketosteroid isomerase (KSI) protein with a C-terminus his(6)-tag was produced following insoluble expression using Escherichia coli. A simple, integrated process was...

Coupling of chemical extraction and expanded-bed adsorption for simplified inclusion-body processing: Optimization using surface plasmon resonance (2003)

Choe, WS, Clemmitt, RH, Chase, HA, Middelberg, APJ

Integration of the chemical extraction of recombinant inclusion-body protein from Escherichia coli, and its recovery by metal-affinity expanded-bed adsorption (IMAC-EBA) under denaturing conditions,...