Department of Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, (2008)
Helga Westers, Ronald Dorenbos, Jorrit Kabel, Tony Flanagan, Kevin M. Devine, ...
Reconstructing the Bacillus genome (Westers et al.) Bacterial genomes contain 250 to 500 essential genes as suggested by single gene disruptions and theoretical considerations. If this view is...
Darmon, Elise, Dorenbos, Ronald, Meens, Jochen, Freudl, Roland, Antelmann, Haike, Hecker, Michael, ...
The gram-positive bacterium Bacillus subtilis secretes high levels of proteins into its environment. Most of these secretory proteins are exported from the cytoplasm in an unfolded state and have to...
Tjalsma, Harold, Antelmann, Haike, Jongbloed, Jan D.H., Braun, Peter G., Darmon, Elise, Dorenbos, Ronald, ...
The extracellular proteome of Bacillus subtilis under secretion stress conditions (2003)
Antelmann, Haike, Darmon, Elise, Noone, David, Veening, Jan-Willem, Westers, Helga, Bron, Sierd, ...
The accumulation of malfolded proteins in the cell envelope of the Gram-positive eubacterium Bacillus subtilis was previously shown to provoke a so-called secretion stress response. In the present...
Genome Engineering Reveals Large Dispensable Regions in Bacillus subtilis (2003)
Westers, Helga, Dorenbos, Ronald, Dijl, Jan Maarten Van, Kabel, Jorrit, Flanagan, Tony, Devine, Kevin M., ...
Bacterial genomes contain 250 to 500 essential genes, as suggested by single gene disruptions and theoretical considerations. If this view is correct, the remaining nonessential genes of an organism,...
Genome Engineering Reveals Large Dispensable Regions in Bacillus subtilis (2003)
Westers, Helga, Dorenbos, Ronald, Van Dijl, Jan Maarten, Kabel, Jorrit, Flanagan, Tony, Devine, Kevin M., ...
Bacterial genomes contain 250 to 500 essential genes as suggested by single gene disruptions and theoretical considerations. If this view is correct, the remaining non-essential genes of an organism,...
Genome Engineering Reveals Large Dispensable Regions in Bacillus subtilis (2003)
Westers, Helga, Dorenbos, Ronald, Van Dijl, Jan Maarten, Kabel, Jorrit, Flanagan, Tony, Devine, Kevin M., ...
Bacterial genomes contain 250 to 500 essential genes, as suggested by single gene disruptions and theoretical considerations. If this view is correct, the remaining nonessential genes of an organism,...
Genome Engineering Reveals Large Dispensable Regions in Bacillus subtilis (2003)
Westers, Helga, Dorenbos, Ronald, Van Dijl, Jan Maarten, Kabel, Jorrit, Flanagan, Tony, Devine, Kevin M., ...
Bacterial genomes contain 250 to 500 essential genes as suggested by single gene disruptions and theoretical considerations. If this view is correct, the remaining non-essential genes of an organism,...
A Proteomic View on Genome-Based Signal Peptide Predictions (2001)
Antelmann, Haike, Tjalsma, Harold, Voigt, Birgit, Ohlmeier, Steffen, Bron, Sierd, Van Dijl, Jan Maarten, ...
Greifswald, University, Diss., 1998.
Antelmann, Haike, Scharf, Christian, Hecker, Michael
The phosphate starvation response in Bacillus subtilis was analyzed using two-dimensional (2D) polyacrylamide gel electrophoresis of cell extracts and supernatants from phosphate-starved cells. Most...
One of Two OsmC Homologs in Bacillus subtilis Is Part of the ςB-Dependent General Stress Regulon
Völker, Uwe, Andersen, Kasper Krogh, Antelmann, Haike, Devine, Kevin M., Hecker, Michael
In this report we present the identification and analysis of two Bacillus subtilis genes, yklA and ykzA, which are homologous to the partially RpoS-controlled osmC gene from Escherichia coli. The...
Binding of σA and σB to Core RNA Polymerase after Environmental Stress in Bacillus subtilis
Rollenhagen, Claudia, Antelmann, Haike, Kirstein, Janine, Delumeau, Olivier, Hecker, Michael, Yudkin, Michael D.
In Bacillus subtilis, the alternative sigma factor σB is activated in response to environmental stress or energy depletion. The general stress regulon under the control of σB provides the cell with...
Proteomics of Protein Secretion by Bacillus subtilis: Separating the “Secrets” of the Secretome
Tjalsma, Harold, Antelmann, Haike, Jongbloed, Jan D.H., Braun, Peter G., Darmon, Elise, Dorenbos, Ronald, ...
Secretory proteins perform a variety of important “remote-control” functions for bacterial survival in the environment. The availability of complete genome sequences has allowed us to make...
Zanen, Geeske, Antelmann, Haike, Westers, Helga, Hecker, Michael, Van Dijl, Jan Maarten, Quax, Wim J.
Bacillus subtilis contains three proteins of the signal recognition particle-GTPase family known as Ffh, FtsY, and FlhF. Here we show that FlhF is dispensable for protein secretion, unlike Ffh and...
Westers, Helga, Braun, Peter G., Westers, Lidia, Antelmann, Haike, Hecker, Michael, Jongbloed, Jan D. H., ...
Small lipases of Bacillus species, such as LipA from Bacillus subtilis, have a high potential for industrial applications. Recent studies showed that deletion of six AT-rich islands from the B....
Role of the Fur Regulon in Iron Transport in Bacillus subtilis
Ollinger, Juliane, Song, Kyung-Bok, Antelmann, Haike, Hecker, Michael, Helmann, John D.
The Bacillus subtilis ferric uptake regulator (Fur) protein mediates the iron-dependent repression of at least 20 operons encoding ∼40 genes. We investigated the physiological roles of...
Antelmann, Haike, Scharf, Christian, Hecker, Michael
The phosphate starvation response in Bacillus subtilis was analyzed using two-dimensional (2D) polyacrylamide gel electrophoresis of cell extracts and supernatants from phosphate-starved cells. Most...
One of Two OsmC Homologs in Bacillus subtilis Is Part of the ςB-Dependent General Stress Regulon
Völker, Uwe, Andersen, Kasper Krogh, Antelmann, Haike, Devine, Kevin M., Hecker, Michael
In this report we present the identification and analysis of two Bacillus subtilis genes, yklA and ykzA, which are homologous to the partially RpoS-controlled osmC gene from Escherichia coli. The...
Binding of σA and σB to Core RNA Polymerase after Environmental Stress in Bacillus subtilis
Rollenhagen, Claudia, Antelmann, Haike, Kirstein, Janine, Delumeau, Olivier, Hecker, Michael, Yudkin, Michael D.
In Bacillus subtilis, the alternative sigma factor σB is activated in response to environmental stress or energy depletion. The general stress regulon under the control of σB provides the cell with...
Proteomics of Protein Secretion by Bacillus subtilis: Separating the “Secrets” of the Secretome
Tjalsma, Harold, Antelmann, Haike, Jongbloed, Jan D.H., Braun, Peter G., Darmon, Elise, Dorenbos, Ronald, ...
Secretory proteins perform a variety of important “remote-control” functions for bacterial survival in the environment. The availability of complete genome sequences has allowed us to make...
Zanen, Geeske, Antelmann, Haike, Westers, Helga, Hecker, Michael, Van Dijl, Jan Maarten, Quax, Wim J.
Bacillus subtilis contains three proteins of the signal recognition particle-GTPase family known as Ffh, FtsY, and FlhF. Here we show that FlhF is dispensable for protein secretion, unlike Ffh and...
Westers, Helga, Braun, Peter G., Westers, Lidia, Antelmann, Haike, Hecker, Michael, Jongbloed, Jan D. H., ...
Small lipases of Bacillus species, such as LipA from Bacillus subtilis, have a high potential for industrial applications. Recent studies showed that deletion of six AT-rich islands from the B....
Role of the Fur Regulon in Iron Transport in Bacillus subtilis
Ollinger, Juliane, Song, Kyung-Bok, Antelmann, Haike, Hecker, Michael, Helmann, John D.
The Bacillus subtilis ferric uptake regulator (Fur) protein mediates the iron-dependent repression of at least 20 operons encoding ∼40 genes. We investigated the physiological roles of...
Darmon, Elise , Dorenbos, Ronald, Meens, Jochen, Freudl, Roland, Antelmann, Haike, Hecker, Michael, ...
The gram-positive bacterium Bacillus subtilis secretes high levels of proteins into its environment. Most of these secretory proteins are exported from the cytoplasm in an unfolded state and have to...
Gaballa, Ahmed, Antelmann, Haike, Aguilar, Claudio, Khakh, Sukhjit K., Song, Kyung-Bok, Smaldone, Gregory T., ...
Regulation of bacterial iron homeostasis is often controlled by the iron-sensing ferric uptake repressor (Fur). The Bacillus subtilis Fur protein acts as an iron-dependent repressor for siderophore...
MacLellan, Shawn R., Helmann, John D., Antelmann, Haike
YvrI is a recently identified alternative σ factor in Bacillus subtilis that requires the coactivator YvrHa to activate transcription. Previously, a strain engineered to overproduce YvrI was found...