Hajime Tokuda

Large-scale preparation of the homogeneous LolA lipoprotein complex and efficient in vitro transfer of lipoproteins to the outer membrane in a LolB-dependent manner (2007)

Watanabe, Shoji, Oguchi, Yuki, Yokota, Naoko, Tokuda, Hajime

An ATP-binding cassette transporter LolCDE complex of Escherichia coli releases lipoproteins destined to the outer membrane from the inner membrane as a complex with a periplasmic chaperone, LolA....

Membrane Topology Inversion of SecG Detected by Labeling with a Membrane-Impermeable Sulfhydryl Reagent that Causes a Close Association of SecG with SecA (2002)

Nagamori, Shushi, Nishiyama, Ken-ichi, Tokuda, Hajime

SecG stimulates protein translocation in Escherichia coli by facilitating the membrane insertion-deinsertion cycle of SecA. SecG was previously shown to undergo membrane topology inversion, since...

Two SecG Molecules Present in a Single Protein Translocation Machinery Are Functional Even after Crosslinking (2000)

Nagamori, Shushi, Nishiyama, Ken-ichi, Tokuda, Hajime

SecG, a membrane component of the protein translocation apparatus of Escherichia coli, undergoes membrane topology inversion, which is coupled to the membrane insertion and deinsertion cycle of SecA....

Roles of the Conserved Cytoplasmic Region and Non-Conserved Carboxy-Terminal Region of SecE in Escherichia coli Protein Translocase (1996)

Kontinen, Vesa P., Yamanaka, Miyuki, Nishiyama, Ken-ichi, Tokuda, Hajime

SecE, an essential membrane component of the Escherichia coli protein translocase, consists of 127 ami no acid residues. Only a part of the second putative cytoplasmic region comprising some 13...

Fluorescence Quenching Studies on the Characterization of Energy Generated at the NADH:Quinone Oxidoreductase and Quinol Oxidase Segments of Marine Bacteria (1991)

Kim, Young Jae, Mizushima, Shoji, Tokuda, Hajime

Generation of membrane potential (inside-positive) and ΔpH (inside-acidic) at two kinds of NADH:quinone oxidoreductase segments, the Na+-motive segment and another segment, of Vibrio...

Roles of the Respiratory Na+ Pump in Bioenergetics of Vibrio alginolyticus (1988)

Tokuda, Hajime, Asano, Makoto, Shimamura, Yoshiki, Unemoto, Tsutomu, Sugiyama, Shigeru, Imae, Yasuo

Bioenergetic characteristics of Na+ pump-defective mutants of a marine bacterium Vibrio alginolyticus were compared with those of the wild type and revertant. Generation of membrane potential and...

Overexpression of yccL (gnsA) and ydfY (gnsB) Increases Levels of Unsaturated Fatty Acids and Suppresses both the Temperature-Sensitive fabA6 Mutation and Cold-Sensitive secG Null Mutation of Escherichia coli

Sugai, Rie, Shimizu, Hisayo, Nishiyama, Ken-ichi, Tokuda, Hajime

A multicopy suppressor of the cold-sensitive secG null mutation was isolated. The suppressor contained sfa and yccL, the former of which has been reported to be a multicopy suppressor of the fabA6...

Deletion of lolB, Encoding an Outer Membrane Lipoprotein, Is Lethal for Escherichia coli and Causes Accumulation of Lipoprotein Localization Intermediates in the Periplasm

Tanaka, Kimie, Matsuyama, Shin-Ichi, Tokuda, Hajime

Outer membrane lipoproteins of Escherichia coli are released from the inner membrane upon the formation of a complex with a periplasmic chaperone, LolA, followed by localization to the outer...

Elucidation of the function of lipoprotein-sorting signals that determine membrane localization

Masuda, Kazuhiro, Matsuyama, Shin-ichi, Tokuda, Hajime

Escherichia coli lipoproteins are anchored to the inner or outer membrane depending on the residue at position 2. Aspartate at this position makes lipoproteins specific to the inner membrane, whereas...

Disruption of lolCDE, Encoding an ATP-Binding Cassette Transporter, Is Lethal for Escherichia coli and Prevents Release of Lipoproteins from the Inner Membrane

Narita, Shin-ichiro, Tanaka, Kimie, Matsuyama, Shin-ichi, Tokuda, Hajime

ATP-binding cassette transporter LolCDE was previously identified, by using reconstituted proteoliposomes, as an apparatus catalyzing the release of outer membrane-specific lipoproteins from the...

Crystal structures of bacterial lipoprotein localization factors, LolA and LolB

Takeda, Kazuki, Miyatake, Hideyuki, Yokota, Naoko, Matsuyama, Shin-ichi, Tokuda, Hajime, Miki, Kunio

Lipoproteins having a lipid-modified cysteine at the N-terminus are localized on either the inner or the outer membrane of Escherichia coli depending on the residue at position 2. Five Lol proteins...

Overexpression of gnsA, a Multicopy Suppressor of the secG Null Mutation, Increases Acidic Phospholipid Contents by Inhibiting Phosphatidylethanolamine Synthesis at Low Temperatures

Sugai, Rie, Shimizu, Hisayo, Nishiyama, Ken-ichi, Tokuda, Hajime

GnsA overproduction was previously found to suppress both the secG null mutation and the fabA6 mutation in Escherichia coli by increasing the unsaturated fatty acid contents. We report here that it...

Genetic Analysis of the Mode of Interplay between an ATPase Subunit and Membrane Subunits of the Lipoprotein-Releasing ATP-Binding Cassette Transporter LolCDE†

Ito, Yasuko, Matsuzawa, Hitomi, Matsuyama, Shin-ichi, Narita, Shin-ichiro, Tokuda, Hajime

The LolCDE complex, an ATP-binding cassette (ABC) transporter, releases lipoproteins from the inner membrane, thereby initiating lipoprotein sorting to the outer membrane of Escherichia coli. The...

Overexpression of yccL (gnsA) and ydfY (gnsB) Increases Levels of Unsaturated Fatty Acids and Suppresses both the Temperature-Sensitive fabA6 Mutation and Cold-Sensitive secG Null Mutation of Escherichia coli

Sugai, Rie, Shimizu, Hisayo, Nishiyama, Ken-ichi, Tokuda, Hajime

A multicopy suppressor of the cold-sensitive secG null mutation was isolated. The suppressor contained sfa and yccL, the former of which has been reported to be a multicopy suppressor of the fabA6...

Deletion of lolB, Encoding an Outer Membrane Lipoprotein, Is Lethal for Escherichia coli and Causes Accumulation of Lipoprotein Localization Intermediates in the Periplasm

Tanaka, Kimie, Matsuyama, Shin-Ichi, Tokuda, Hajime

Outer membrane lipoproteins of Escherichia coli are released from the inner membrane upon the formation of a complex with a periplasmic chaperone, LolA, followed by localization to the outer...

Elucidation of the function of lipoprotein-sorting signals that determine membrane localization

Masuda, Kazuhiro, Matsuyama, Shin-ichi, Tokuda, Hajime

Escherichia coli lipoproteins are anchored to the inner or outer membrane depending on the residue at position 2. Aspartate at this position makes lipoproteins specific to the inner membrane, whereas...

Disruption of lolCDE, Encoding an ATP-Binding Cassette Transporter, Is Lethal for Escherichia coli and Prevents Release of Lipoproteins from the Inner Membrane

Narita, Shin-ichiro, Tanaka, Kimie, Matsuyama, Shin-ichi, Tokuda, Hajime

ATP-binding cassette transporter LolCDE was previously identified, by using reconstituted proteoliposomes, as an apparatus catalyzing the release of outer membrane-specific lipoproteins from the...

Crystal structures of bacterial lipoprotein localization factors, LolA and LolB

Takeda, Kazuki, Miyatake, Hideyuki, Yokota, Naoko, Matsuyama, Shin-ichi, Tokuda, Hajime, Miki, Kunio

Lipoproteins having a lipid-modified cysteine at the N-terminus are localized on either the inner or the outer membrane of Escherichia coli depending on the residue at position 2. Five Lol proteins...

Overexpression of gnsA, a Multicopy Suppressor of the secG Null Mutation, Increases Acidic Phospholipid Contents by Inhibiting Phosphatidylethanolamine Synthesis at Low Temperatures

Sugai, Rie, Shimizu, Hisayo, Nishiyama, Ken-ichi, Tokuda, Hajime

GnsA overproduction was previously found to suppress both the secG null mutation and the fabA6 mutation in Escherichia coli by increasing the unsaturated fatty acid contents. We report here that it...

Genetic Analysis of the Mode of Interplay between an ATPase Subunit and Membrane Subunits of the Lipoprotein-Releasing ATP-Binding Cassette Transporter LolCDE†

Ito, Yasuko, Matsuzawa, Hitomi, Matsuyama, Shin-ichi, Narita, Shin-ichiro, Tokuda, Hajime

The LolCDE complex, an ATP-binding cassette (ABC) transporter, releases lipoproteins from the inner membrane, thereby initiating lipoprotein sorting to the outer membrane of Escherichia coli. The...

Large-scale preparation of the homogeneous LolA–lipoprotein complex and efficient in vitro transfer of lipoproteins to the outer membrane in a LolB-dependent manner

Watanabe, Shoji, Oguchi, Yuki, Yokota, Naoko, Tokuda, Hajime

An ATP-binding cassette transporter LolCDE complex of Escherichia coli releases lipoproteins destined to the outer membrane from the inner membrane as a complex with a periplasmic chaperone, LolA....

Purification, crystallization and preliminary X-ray crystallographic analysis of the outer membrane lipoprotein NlpE from Escherichia coli

Hirano, Yu, Hossain, Md. Motarab, Takeda, Kazuki, Tokuda, Hajime, Miki, Kunio

A water-soluble mutant of the outer membrane lipoprotein NlpE has been overexpressed, purified and crystallized. Diffraction data from two crystal forms obtained under two different conditions were...

Molecular Events Involved in a Single Cycle of Ligand Transfer from an ATP Binding Cassette Transporter, LolCDE, to a Molecular Chaperone, LolA*

Taniguchi, Naohiro, Tokuda, Hajime

An ATP binding cassette transporter LolCDE complex releases lipoproteins from the inner membrane of Escherichia coli in an ATP-dependent manner, leading to the formation of a complex between a...

Model of mouth-to-mouth transfer of bacterial lipoproteins through inner membrane LolC, periplasmic LolA, and outer membrane LolB

Okuda, Suguru, Tokuda, Hajime

Outer membrane-specific lipoproteins in Escherichia coli are released from the inner membrane by an ATP-binding cassette transporter, the LolCDE complex, which causes the formation of a soluble...