Pietiäinen, Milla, François, Patrice, Hyyryläinen, Hanne-Leena, Tangomo, Manuela, Sass, Vera, Sahl, Hans-Georg, ...
Abstract Background Understanding how pathogens respond to antimicrobial peptides, and how this compares to currently available antibiotics, is crucial for optimizing antimicrobial therapy....
Sass, Peter, Jansen, Andrea, Szekat, Christiane, Sass, Vera, Sahl, Hans-Georg, Bierbaum, Gabriele
Abstract Background The lantibiotic mersacidin is an antimicrobial peptide of 20 amino acids that is ribosomally produced by Bacillus sp. strain HIL Y-85,54728. Mersacidin acts by complexing the...
Kruszewska, Danuta, Sahl, Hans-Georg, Bierbaum, Gabriele, Pag, Ulrike, Hynes, Sean O., Ljungh, Asa
Abstract is not available
Kruszewska, Danuta, Sahl, Hans-Georg, Bierbaum, Gabriele, Pag, Ulrike, Hynes, Sean O., Ljungh, Åsa
Objectives: Methicillin-resistant Staphylococcus aureus (MRSA) often colonize the anterior nares, and nasal carriage remains the main source of bacterial dissemination. The aim of this study was to...
Kruszewska, Danuta, Sahl, Hans-Georg, Bierbaum, Gabriele, Pag, Ulrike, Hynes, Sean O., Ljungh, Åsa
Objectives: Methicillin-resistant Staphylococcus aureus (MRSA) often colonize the anterior nares, and nasal carriage remains the main source of bacterial dissemination. The aim of this study was to...
Wiedemann, Imke, Breukink, Eefjan, Kraaij, Cindy Van, Kuipers, Oscar P., Bierbaum, Gabriele, Kruijff, Ben De, ...
Unlike numerous pore-forming amphiphilic peptide antibiotics, the lantibiotic nisin is active in nanomolar concentrations, which results from its ability to use the lipid-bound cell wall precursor...
Protein engineering of lantibiotics (1996)
Kuipers, Oscar P., Bierbaum, Gabriele, Ottenwälder, Birgit, Dodd, Helen M., Horn, Nicky, Metzger, Jörg, ...
Whereas protein engineering of enzymes and structural proteins nowadays is an established research tool for studying structure-function relationships of polypeptides and for improving their...
Mechanistic Studies of Lantibiotic-Induced Permeabilization of Phospholipid Vesicles (1995)
Driessen, Arnold J.M., Kuiper, Wieny, Kamp, Mart Van De, Sahl, Hans-Georg, Konings, Ruud N.H., ...
Nisin is a cationic polycyclic bacteriocin secreted by some lactic acid bacteria. Nisin has previously been shown to permeabilize liposomes. The interaction of nisin was analyzed with liposomes...
Kamp, Mart Van De, Konings, Ruud N.H., Bierbaum, Gabriele, Sahl, Hans-Georg, Kuipers, Oscar P., ...
Lantibiotics are bacteriocins that contain unusual amino acids such as lanthionines and α,β-didehydro residues generated by posttranslational modification of a ribosomally synthesized precursor...
Bonn, Univ., Habil.-Schr., 1983 (Nicht f.d. Austausch).
Two-Component Anti-Staphylococcus aureus Lantibiotic Activity Produced by Staphylococcus aureus C55
Sahl, Hans-Georg, Tagg, John R.
Staphylococcus aureus C55 was shown to produce bacteriocin activity comprising three distinct peptide components, termed staphylococcins C55α, C55β, and C55γ. The three peptides were purified to...
Molecular Analysis of Expression of the Lantibiotic Pep5 Immunity Phenotype
Pag, Ulrike, Heidrich, Christoph, Bierbaum, Gabriele, Sahl, Hans-Georg
The lantibiotic Pep5 is produced by Staphylococcus epidermidis 5. Within its biosynthetic gene cluster, the immunity gene pepI, providing producer self-protection, is localized upstream of the...
Sahl, Hans-Georg, Tagg, John R.
The production of exfoliative toxin B (ET-B), but not ET-A, was shown to be specifically associated with production of a highly conserved two-component lantibiotic peptide system in phage group II...
The Lantibiotic Mersacidin Inhibits Peptidoglycan Synthesis by Targeting Lipid II
Brötz, Heike, Bierbaum, Gabriele, Leopold, Klaus, Reynolds, Peter E., Sahl, Hans-Georg
The lantibiotic mersacidin exerts its bactericidal action by inhibition of peptidoglycan biosynthesis. It interferes with the membrane-associated transglycosylation reaction; during this step the...
Heidrich, Christoph, Pag, Ulrike, Josten, Michaele, Metzger, Jörg, Jack, Ralph W., Bierbaum, Gabriele, ...
Epicidin 280 is a novel type A lantibiotic produced by Staphylococcus epidermidis BN 280. During C18 reverse-phase high-performance liquid chromatography two epicidin 280 peaks were obtained; the two...
Guder, André, Schmitter, Tim, Wiedemann, Imke, Sahl, Hans-Georg, Bierbaum, Gabriele
The lantibiotic mersacidin is an antimicrobial peptide of 20 amino acids which inhibits bacterial cell wall biosynthesis by binding to the precursor molecule lipid II and which is produced by...
Mode of Action of the Antimicrobial Peptide Aureocin A53 from Staphylococcus aureus
Netz, Daili Jacqueline Aguilar, Sahl, Hans-Georg
We investigated the mode of action of aureocin A53 on living bacterial cells and model membranes. Aureocin A53 acted bactericidally against Staphylococcus simulans 22, with >90% of the cells killed...
Lipid II-Mediated Pore Formation by the Peptide Antibiotic Nisin: a Black Lipid Membrane Study
Wiedemann, Imke, Benz, Roland, Sahl, Hans-Georg
The antibiotic peptide nisin is the first known lantibiotic that uses a docking molecule within the bacterial cytoplasmic membrane for pore formation. Through specific interaction with the cell wall...
Hoffmann, Anja, Schneider, Tanja, Pag, Ulrike, Sahl, Hans-Georg
Pep5 is a cationic pore-forming lantibiotic produced by Staphylococcus epidermidis strain 5. The producer strain protects itself from the lethal action of its own bacteriocin through the...
Zelezetsky, Igor, Pacor, Sabrina, Pag, Ulrike, Papo, Niv, Shai, Yechiel, Sahl, Hans-Georg, ...
A novel method, based on the rational and systematic modulation of macroscopic structural characteristics on a template originating from a large number of natural, cell-lytic, amphipathic α-helical...
Bayer, Arnold S., McNamara, Peter, Yeaman, Michael R., Lucindo, Natalie, Jones, Tiffanny, Cheung, Ambrose L., ...
The cationic molecule thrombin-induced platelet microbicidal protein 1 (tPMP-1) exerts potent activity against Staphylococcus aureus. We previously reported that a Tn551 S. aureus transposon mutant,...
Bonelli, Raquel Regina, Schneider, Tanja, Sahl, Hans-Georg, Wiedemann, Imke
The activity of lanthionine-containing peptide antibiotics (lantibiotics) is based on different killing mechanisms which may be combined in one molecule. The prototype lantibiotic nisin inhibits...
Chatterjee, Indranil, Somerville, Greg A., Heilmann, Christine, Sahl, Hans-Georg, Maurer, Hans H., Herrmann, Mathias
Pharmaceuticals, culture media used for in vitro diagnostics and research, human body fluids, and environments can retain very low ethanol concentrations (VLEC) (≤0.1%, vol/vol). In contrast to the...
Lipid II-Based Antimicrobial Activity of the Lantibiotic Plantaricin C
Wiedemann, Imke, Böttiger, Tim, Bonelli, Raquel Regina, Schneider, Tanja, Sahl, Hans-Georg, Martínez, Beatriz
We analyzed the mode of action of the lantibiotic plantaricin C (PlnC), produced by Lactobacillus plantarum LL441. Compared to the well-characterized type A lantibiotic nisin and type B lantibiotic...
Two-Component Anti-Staphylococcus aureus Lantibiotic Activity Produced by Staphylococcus aureus C55
Sahl, Hans-Georg, Tagg, John R.
Staphylococcus aureus C55 was shown to produce bacteriocin activity comprising three distinct peptide components, termed staphylococcins C55α, C55β, and C55γ. The three peptides were purified to...
Molecular Analysis of Expression of the Lantibiotic Pep5 Immunity Phenotype
Pag, Ulrike, Heidrich, Christoph, Bierbaum, Gabriele, Sahl, Hans-Georg
The lantibiotic Pep5 is produced by Staphylococcus epidermidis 5. Within its biosynthetic gene cluster, the immunity gene pepI, providing producer self-protection, is localized upstream of the...
Sahl, Hans-Georg, Tagg, John R.
The production of exfoliative toxin B (ET-B), but not ET-A, was shown to be specifically associated with production of a highly conserved two-component lantibiotic peptide system in phage group II...
The Lantibiotic Mersacidin Inhibits Peptidoglycan Synthesis by Targeting Lipid II
Brötz, Heike, Bierbaum, Gabriele, Leopold, Klaus, Reynolds, Peter E., Sahl, Hans-Georg
The lantibiotic mersacidin exerts its bactericidal action by inhibition of peptidoglycan biosynthesis. It interferes with the membrane-associated transglycosylation reaction; during this step the...
Heidrich, Christoph, Pag, Ulrike, Josten, Michaele, Metzger, Jörg, Jack, Ralph W., Bierbaum, Gabriele, ...
Epicidin 280 is a novel type A lantibiotic produced by Staphylococcus epidermidis BN 280. During C18 reverse-phase high-performance liquid chromatography two epicidin 280 peaks were obtained; the two...
Guder, André, Schmitter, Tim, Wiedemann, Imke, Sahl, Hans-Georg, Bierbaum, Gabriele
The lantibiotic mersacidin is an antimicrobial peptide of 20 amino acids which inhibits bacterial cell wall biosynthesis by binding to the precursor molecule lipid II and which is produced by...
Mode of Action of the Antimicrobial Peptide Aureocin A53 from Staphylococcus aureus
Netz, Daili Jacqueline Aguilar, Sahl, Hans-Georg
We investigated the mode of action of aureocin A53 on living bacterial cells and model membranes. Aureocin A53 acted bactericidally against Staphylococcus simulans 22, with >90% of the cells killed...
Lipid II-Mediated Pore Formation by the Peptide Antibiotic Nisin: a Black Lipid Membrane Study
Wiedemann, Imke, Benz, Roland, Sahl, Hans-Georg
The antibiotic peptide nisin is the first known lantibiotic that uses a docking molecule within the bacterial cytoplasmic membrane for pore formation. Through specific interaction with the cell wall...
Hoffmann, Anja, Schneider, Tanja, Pag, Ulrike, Sahl, Hans-Georg
Pep5 is a cationic pore-forming lantibiotic produced by Staphylococcus epidermidis strain 5. The producer strain protects itself from the lethal action of its own bacteriocin through the...
Zelezetsky, Igor, Pacor, Sabrina, Pag, Ulrike, Papo, Niv, Shai, Yechiel, Sahl, Hans-Georg, ...
A novel method, based on the rational and systematic modulation of macroscopic structural characteristics on a template originating from a large number of natural, cell-lytic, amphipathic α-helical...
Bayer, Arnold S., McNamara, Peter, Yeaman, Michael R., Lucindo, Natalie, Jones, Tiffanny, Cheung, Ambrose L., ...
The cationic molecule thrombin-induced platelet microbicidal protein 1 (tPMP-1) exerts potent activity against Staphylococcus aureus. We previously reported that a Tn551 S. aureus transposon mutant,...
Bonelli, Raquel Regina, Schneider, Tanja, Sahl, Hans-Georg, Wiedemann, Imke
The activity of lanthionine-containing peptide antibiotics (lantibiotics) is based on different killing mechanisms which may be combined in one molecule. The prototype lantibiotic nisin inhibits...
Chatterjee, Indranil, Somerville, Greg A., Heilmann, Christine, Sahl, Hans-Georg, Maurer, Hans H., Herrmann, Mathias
Pharmaceuticals, culture media used for in vitro diagnostics and research, human body fluids, and environments can retain very low ethanol concentrations (VLEC) (≤0.1%, vol/vol). In contrast to the...
Lipid II-Based Antimicrobial Activity of the Lantibiotic Plantaricin C
Wiedemann, Imke, Böttiger, Tim, Bonelli, Raquel Regina, Schneider, Tanja, Sahl, Hans-Georg, Martínez, Beatriz
We analyzed the mode of action of the lantibiotic plantaricin C (PlnC), produced by Lactobacillus plantarum LL441. Compared to the well-characterized type A lantibiotic nisin and type B lantibiotic...
Jones, Tiffanny, Yeaman, Michael R., Sakoulas, George, Yang, Soo-Jin, Proctor, Richard A., Sahl, Hans-Georg, ...
Increasingly frequent reports have described the in vivo loss of daptomycin susceptibility in association with clinical treatment failures. The mechanism(s) of daptomycin resistance is not well...
Insights into the Mode of Action of Chitosan as an Antibacterial Compound▿ †
Raafat, Dina, Von Bargen, Kristine, Haas, Albert, Sahl, Hans-Georg
Chitosan is a polysaccharide biopolymer that combines a unique set of versatile physicochemical and biological characteristics which allow for a wide range of applications. Although its antimicrobial...
Martínez, Beatriz, Böttiger, Tim, Schneider, Tanja, Rodríguez, Ana, Sahl, Hans-Georg, Wiedemann, Imke
Lactococcin 972 (Lcn972) is a nonlantibiotic bacteriocin that inhibits septum biosynthesis in Lactococcus lactis rather than forming pores in the cytoplasmic membrane. In this study, a deeper...
Insights into the Mode of Action of Chitosan as an Antibacterial Compound
Raafat, Dina, Von Bargen, Kristine, Haas, Albert, Sahl, Hans-Georg