Regulation of ubiquitin-binding proteins by monoubiquitination (2006)
Hoeller, Daniela, Crosetto, Nicola, Blagoev, Blagoy, Raiborg, Camilla, Tikkanen, Ritva, Wagner, Sebastian, ...
Proteins containing ubiquitin-binding domains (UBDs) interact with ubiquitinated targets and regulate diverse biological processes, including endocytosis, signal transduction, transcription and DNA...
Early endosomal regulation of Smad-dependent signaling in endothelial cells (2002)
Panopoulou, Ekaterini, Gillooly, David J., Wrana, Jeffrey L., Zerial, Marino, Stenmark, Harald, Murphy, Carol, ...
Transforming growth factor beta (TGFbeta) receptors require SARA for phosphorylation of the downstream transducing Smad proteins. SARA, a FYVE finger protein, binds to membrane lipids suggesting that...
Stenmark, Harald, Olkkonen, Vesa M
Summary The Rab family is part of the Ras superfamily of small GTPases. There are at least 60 Rab genes in the human genome, and a number of Rab GTPases are conserved from yeast to humans. The...
Urbé, Sylvie, Mills, Ian G., Stenmark, Harald, Kitamura, Naomi, Clague, Michael J.
Hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs) is a prominent substrate for activated tyrosine kinase receptors that has been proposed to play a role in endosomal membrane...
Kim, Dae Heon, Eu, Young-Jae, Yoo, Cheol Min, Kim, Yong-Woo, Pih, Kyeong Tae, Jin, Jing Bo, ...
Very limited information is available on the role of phosphatidylinositol 3-phosphate (PI[3]P) in vesicle trafficking in plant cells. To investigate the role of PI(3)P during the vesicle trafficking...
Hrs recruits clathrin to early endosomes
Raiborg, Camilla, Grønvold Bache, Kristi, Mehlum, Anja, Stang, Espen, Stenmark, Harald
The hepatocyte growth factor-regulated tyrosine kinase substrate, Hrs, has been implicated in intracellular trafficking and signal transduction. Hrs contains a phosphatidylinositol...
Stenmark, Harald, Olkkonen, Vesa M
The Rab family is part of the Ras superfamily of small GTPases. There are at least 60 Rab genes in the human genome, and a number of Rab GTPases are conserved from yeast to humans. The different Rab...
Localization of phosphatidylinositol 3-phosphate in yeast and mammalian cells
Gillooly, David J., Morrow, Isabel C., Lindsay, Margaret, Gould, Robert, Bryant, Nia J., Gaullier, Jean-Michel, ...
Phosphatidylinositol 3-kinase (PI3K) regulates several vital cellular processes, including signal transduction and membrane trafficking. In order to study the intracellular localization of the PI3K...
Acquisition of Hrs, an Essential Component of Phagosomal Maturation, Is Impaired by Mycobacteria
Vieira, Otilia V., Harrison, Rene E., Scott, Cameron C., Stenmark, Harald, Alexander, David, Liu, Jun, ...
Pathogenic mycobacteria survive within macrophages by precluding the fusion of phagosomes with late endosomes or lysosomes. Because the molecular determinants of normal phagolysosome formation are...
Cbl-dependent Ubiquitination Is Required for Progression of EGF Receptors into Clathrin-coated Pits
Stang, Espen, Blystad, Frøydis D., Kazazic, Maja, Bertelsen, Vibeke, Brodahl, Tonje, Raiborg, Camilla, ...
Ligand binding causes the EGF receptor (EGFR) to become ubiquitinated by Cbl upon association with the adaptor protein Grb2. We have investigated the role of ubiquitin and Grb2 in ligand-induced...
Defective downregulation of receptor tyrosine kinases in cancer
Bache, Kristi G, Slagsvold, Thomas, Stenmark, Harald
Most growth factors control cellular functions by activating specific receptor tyrosine kinases (RTKs). While overactivation of RTK signalling pathways is strongly associated with carcinogenesis, it...
Bache, Kristi G., Slagsvold, Thomas, Cabezas, Alicia, Rosendal, Ken R., Raiborg, Camilla, Stenmark, Harald
The biogenesis of multivesicular bodies and endosomal sorting of membrane cargo are driven forward by the endosomal sorting complexes required for transport, ESCRT-I, -II, and -III. ESCRT-I is...
Bache, Kristi G., Stuffers, Susanne, Malerød, Lene, Slagsvold, Thomas, Raiborg, Camilla, Lechardeur, Delphine, ...
The endosomal sorting complexes required for transport, ESCRT-I, -II, and -III, are thought to mediate the biogenesis of multivesicular endosomes (MVEs) and endosomal sorting of ubiquitinated...
Rusten, Tor Erik, Rodahl, Lina M.W., Pattni, Krupa, Englund, Camilla, Samakovlis, Christos, Dove, Stephen, ...
The trafficking of endocytosed receptors through phosphatidylinositol 3-phosphate [PtdIns(3)P]-containing endosomes is thought to attenuate their signaling. Here, we show that the PtdIns(3)P 5-kinase...
Urbé, Sylvie, Mills, Ian G., Stenmark, Harald, Kitamura, Naomi, Clague, Michael J.
Hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs) is a prominent substrate for activated tyrosine kinase receptors that has been proposed to play a role in endosomal membrane...
Kim, Dae Heon, Eu, Young-Jae, Yoo, Cheol Min, Kim, Yong-Woo, Pih, Kyeong Tae, Jin, Jing Bo, ...
Very limited information is available on the role of phosphatidylinositol 3-phosphate (PI[3]P) in vesicle trafficking in plant cells. To investigate the role of PI(3)P during the vesicle trafficking...
Hrs recruits clathrin to early endosomes
Raiborg, Camilla, Grønvold Bache, Kristi, Mehlum, Anja, Stang, Espen, Stenmark, Harald
The hepatocyte growth factor-regulated tyrosine kinase substrate, Hrs, has been implicated in intracellular trafficking and signal transduction. Hrs contains a phosphatidylinositol...
Stenmark, Harald, Olkkonen, Vesa M
The Rab family is part of the Ras superfamily of small GTPases. There are at least 60 Rab genes in the human genome, and a number of Rab GTPases are conserved from yeast to humans. The different Rab...
Localization of phosphatidylinositol 3-phosphate in yeast and mammalian cells
Gillooly, David J., Morrow, Isabel C., Lindsay, Margaret, Gould, Robert, Bryant, Nia J., Gaullier, Jean-Michel, ...
Phosphatidylinositol 3-kinase (PI3K) regulates several vital cellular processes, including signal transduction and membrane trafficking. In order to study the intracellular localization of the PI3K...
Acquisition of Hrs, an Essential Component of Phagosomal Maturation, Is Impaired by Mycobacteria
Vieira, Otilia V., Harrison, Rene E., Scott, Cameron C., Stenmark, Harald, Alexander, David, Liu, Jun, ...
Pathogenic mycobacteria survive within macrophages by precluding the fusion of phagosomes with late endosomes or lysosomes. Because the molecular determinants of normal phagolysosome formation are...
Cbl-dependent Ubiquitination Is Required for Progression of EGF Receptors into Clathrin-coated Pits
Stang, Espen, Blystad, Frøydis D., Kazazic, Maja, Bertelsen, Vibeke, Brodahl, Tonje, Raiborg, Camilla, ...
Ligand binding causes the EGF receptor (EGFR) to become ubiquitinated by Cbl upon association with the adaptor protein Grb2. We have investigated the role of ubiquitin and Grb2 in ligand-induced...
Defective downregulation of receptor tyrosine kinases in cancer
Bache, Kristi G, Slagsvold, Thomas, Stenmark, Harald
Most growth factors control cellular functions by activating specific receptor tyrosine kinases (RTKs). While overactivation of RTK signalling pathways is strongly associated with carcinogenesis, it...
Bache, Kristi G., Slagsvold, Thomas, Cabezas, Alicia, Rosendal, Ken R., Raiborg, Camilla, Stenmark, Harald
The biogenesis of multivesicular bodies and endosomal sorting of membrane cargo are driven forward by the endosomal sorting complexes required for transport, ESCRT-I, -II, and -III. ESCRT-I is...
Bache, Kristi G., Stuffers, Susanne, Malerød, Lene, Slagsvold, Thomas, Raiborg, Camilla, Lechardeur, Delphine, ...
The endosomal sorting complexes required for transport, ESCRT-I, -II, and -III, are thought to mediate the biogenesis of multivesicular endosomes (MVEs) and endosomal sorting of ubiquitinated...
Rusten, Tor Erik, Rodahl, Lina M.W., Pattni, Krupa, Englund, Camilla, Samakovlis, Christos, Dove, Stephen, ...
The trafficking of endocytosed receptors through phosphatidylinositol 3-phosphate [PtdIns(3)P]-containing endosomes is thought to attenuate their signaling. Here, we show that the PtdIns(3)P 5-kinase...
Endocytic pathways regulate Toll-like receptor 4 signaling and link innate and adaptive immunity
Husebye, Harald, Halaas, Øyvind, Stenmark, Harald, Tunheim, Gro, Sandanger, Øystein, Bogen, Bjarne, ...
Immune responses are initiated when molecules of microbial origin are sensed by the Toll-like receptors (TLRs). We now report the identification of essential molecular components for the trafficking...
CISK attenuates degradation of the chemokine receptor CXCR4 via the ubiquitin ligase AIP4
Slagsvold, Thomas, Marchese, Adriano, Brech, Andreas, Stenmark, Harald
HER2 overexpression in cancers causes hyperactivation of the PI 3-kinase pathway and elevated levels of the chemokine receptor CXCR4, which is strongly associated with increased metastatic potential....
Bjørkøy, Geir, Lamark, Trond, Brech, Andreas, Outzen, Heidi, Perander, Maria, Øvervatn, Aud, ...
Autophagic degradation of ubiquitinated protein aggregates is important for cell survival, but it is not known how the autophagic machinery recognizes such aggregates. In this study, we report that...
Misfolding diverts CFTR from recycling to degradation: quality control at early endosomes
Sharma, Manu, Pampinella, Francesca, Nemes, Csilla, Benharouga, Mohamed, So, Jeffrey, Du, Kai, ...
To investigate the degradation mechanism of misfolded membrane proteins from the cell surface, we used mutant cystic fibrosis transmembrane conductance regulators (CFTRs) exhibiting conformational...
Zacchi, Paola, Stenmark, Harald, Parton, Robert G., Orioli, Donata, Lim, Filip, Giner, Angelika, ...
A key feature of polarized epithelial cells is the ability to maintain the specific biochemical composition of the apical and basolateral plasma membrane domains while selectively allowing transport...
Phosphoinositides and phagocytosis
Gillooly, David J., Simonsen, Anne, Stenmark, Harald
Phosphoinositide 3 kinases (PI3Ks)* are known as regulators of phagocytosis. Recent results demonstrate that class I and III PI3Ks act consecutively in phagosome formation and maturation, and that...
Hrs regulates multivesicular body formation via ESCRT recruitment to endosomes
Bache, Kristi G., Brech, Andreas, Mehlum, Anja, Stenmark, Harald
Hrs and the endosomal sorting complexes required for transport, ESCRT-I, -II, and -III, are involved in the endosomal sorting of membrane proteins into multivesicular bodies and lysosomes or...
Filimonenko, Maria, Stuffers, Susanne, Raiborg, Camilla, Yamamoto, Ai, Malerød, Lene, Fisher, Elizabeth M.C., ...
The endosomal sorting complexes required for transport (ESCRTs) are required to sort integral membrane proteins into intralumenal vesicles of the multivesicular body (MVB). Mutations in the ESCRT-III...
Mechanisms and functions of endocytosis
Miaczynska, Marta, Stenmark, Harald
A recent EMBO-FEBS workshop entitled Endocytic Systems: Mechanism and Function, organized by Howard Riezman in Villars-sur-Ollon (Switzerland), showcased the multifaceted approaches and model systems...
Nezis, Ioannis P., Simonsen, Anne, Sagona, Antonia P., Finley, Kim, Gaumer, Sébastien, Contamine, Didier, ...
p62 has been proposed to mark ubiquitinated protein bodies for autophagic degradation. We report that the Drosophila melanogaster p62 orthologue, Ref(2)P, is a regulator of protein aggregation in the...
Roxrud, Ingrid, Raiborg, Camilla, Pedersen, Nina Marie, Stang, Espen, Stenmark, Harald
Down-regulation of activated and ubiquitinated growth factor (GF) receptors by endocytosis and subsequent lysosomal degradation ensures attenuation of GF signaling. The ubiquitin-binding adaptor...
Umebayashi, Kyohei, Stenmark, Harald, Yoshimori, Tamotsu
c-Cbl is the E3 ubiquitin ligase that ubiquitinates the epidermal growth factor (EGF) receptor (EGFR). On the basis of localization, knockdown, and in vitro activity analyses, we have identified the...