Harold P. Klein

Studies on Acetyl-Coenzyme A Synthetase of Yeast: Inhibition by Long-Chain Acyl-Coenzyme A Esters

Satyanarayana, T., Klein, Harold P.

Long-chain acyl-coenzyme A (CoA) compounds (palmityl, stearyl, and oleyl) were found to be potent inhibitors of acetyl-CoA synthetase (ACS) of Saccharomyces cerevisiae strain LK2G12 from aerobic, but...

Subcellular Sites Involved in Lipid Synthesis in Saccharomyces cerevisiae

Klein, Harold P., Volkmann, Carol M., Leaffer, Morris A.

When the crude ribosomal fraction of Saccharomyces cerevisiae was separated into “light” and “heavy” fractions, fatty acid synthetase was concentrated in the former, whereas acetyl-Coenzyme A...

Activation of Fatty Acid Synthesis in Cell-free Extracts of Saccharomyces cerevisiae

Rasmussen, Roberta K., Klein, Harold P.

Fatty acid synthesis from acetate in extracts of Saccharomyces cerevisiae strain LK2G12 was shown to be stimulated by α-glycerophosphate and citrate, and by a number of compounds related to them....

Mechanism of α-Glycerophosphate Regulation of Acetyl-Coenzyme A Carboxylase of Saccharomyces cerevisiae

Rasmussen, Roberta K., Klein, Harold P.

The mechanism proposed for the activation of animal acetyl-coenzyme A (CoA) carboxylase by α-glycerophosphate, namely, the removal of inhibitory palmityl-CoA via glyceride synthesis, is not the only...

Synthesis of Fatty Acids by Yeast Particles

Klein, Harold P.

Klein, Harold P. (Ames Research Center, Moffett Field, Calif.). Synthesis of fatty acids by yeast particles. J. Bacteriol. 92:130–135. 1966.—When a mitochondria-free homogenate of Saccharomyces...

Fatty Acid Synthetase of Saccharomyces cerevisiae

Klein, Harold P., Volkmann, Carol M., Chao, Fu-Chuan

A light particle fraction of Saccharomyces cerevisiae, obtained from the crude ribosomal material, and containing the fatty acid synthetase, consisted primarily of 27S and 47S components. This...

EFFECTS OF ASPARTATE ON GROWTH AND ON THE SYNTHESIS OF α-AMYLASE IN PSEUDOMONAS SACCHAROPHILA

Eisenstadt, Jerome M., Klein, Harold P.

Eisenstadt, Jerome M. (Brandeis University, Waltham, Mass.), and Harold P. Klein. Effects of aspartate on growth and on the synthesis of α-amylase in Pseudomonas saccharophila. J. Bacteriol....

EVIDENCE FOR THE DE NOVO SYNTHESIS OF THE ALPHA-AMYLASE OF PSEUDOMONAS SACCHAROPHILA

Eisenstadt, Jerome M., Klein, Harold P.

Eisenstadt, Jerome M. (Brandeis University, Waltham, Mass.) and Harold P. Klein. Evidence for the de novo synthesis of the alpha-amylase of Pseudomonas saccharophila. J. Bacteriol. 82:798–807....

Variations in the Localization of Acetyl-Coenzyme A Synthetase in Aerobic Yeast Cells

Klein, Harold P., Jahnke, Linda

In cells of Saccharomyces cerevisiae growing aerobically for 24 hr, acetyl-coenzyme A synthetase [acetate: CoA ligase (AMP), EC 6.2.1.1] was localized principally in the microsomal fraction. On...

Membranes of Saccharomyces cerevisiae

Klein, Harold P., Volkmann, Carol, Weibel, Joseph

A crude small particle pellet, obtained from postmitochondrial supernatant fractions of Saccharomyces cerevisiae, contains about half the ergosterol and phospholipid of crude cell homogenates. Most...

Cellular Localization of Acetyl-Coenzyme A Synthetase in Yeast

Klein, Harold P., Jahnke, Linda

In cells of Saccharomyces cerevisiae grown with glucose in standing cultures, the microsomal fraction had the highest specific activity for acetyl-coenzyme A synthetase and contained the greatest...

Nature of Particles Involved in Lipid Synthesis in Yeast

Klein, Harold P.

Klein, Harold P. (Ames Research Center, Moffett Field, Calif.). Nature of particles involved in lipid synthesis in yeast. J. Bacteriol. 90:227–234. 1965.—Mitochondria-free homogenates of...

Effects of α-Glycerophosphate and of Palmityl-Coenzyme A on Lipid Synthesis in Yeast Extracts

White, David, Klein, Harold P.

White, David (Ames Research Center, Moffett Field, Calif.), and Harold P. Klein. Effects of α-glycerophosphate and of palmityl-coenzyme A on lipid synthesis in yeast extracts. J. Bacteriol....

Studies on Acetyl-Coenzyme A Synthetase of Yeast: Inhibition by Long-Chain Acyl-Coenzyme A Esters

Satyanarayana, T., Klein, Harold P.

Long-chain acyl-coenzyme A (CoA) compounds (palmityl, stearyl, and oleyl) were found to be potent inhibitors of acetyl-CoA synthetase (ACS) of Saccharomyces cerevisiae strain LK2G12 from aerobic, but...

Subcellular Sites Involved in Lipid Synthesis in Saccharomyces cerevisiae

Klein, Harold P., Volkmann, Carol M., Leaffer, Morris A.

When the crude ribosomal fraction of Saccharomyces cerevisiae was separated into “light” and “heavy” fractions, fatty acid synthetase was concentrated in the former, whereas acetyl-Coenzyme A...

Activation of Fatty Acid Synthesis in Cell-free Extracts of Saccharomyces cerevisiae

Rasmussen, Roberta K., Klein, Harold P.

Fatty acid synthesis from acetate in extracts of Saccharomyces cerevisiae strain LK2G12 was shown to be stimulated by α-glycerophosphate and citrate, and by a number of compounds related to them....

Mechanism of α-Glycerophosphate Regulation of Acetyl-Coenzyme A Carboxylase of Saccharomyces cerevisiae

Rasmussen, Roberta K., Klein, Harold P.

The mechanism proposed for the activation of animal acetyl-coenzyme A (CoA) carboxylase by α-glycerophosphate, namely, the removal of inhibitory palmityl-CoA via glyceride synthesis, is not the only...

Synthesis of Fatty Acids by Yeast Particles

Klein, Harold P.

Klein, Harold P. (Ames Research Center, Moffett Field, Calif.). Synthesis of fatty acids by yeast particles. J. Bacteriol. 92:130–135. 1966.—When a mitochondria-free homogenate of Saccharomyces...

Fatty Acid Synthetase of Saccharomyces cerevisiae

Klein, Harold P., Volkmann, Carol M., Chao, Fu-Chuan

A light particle fraction of Saccharomyces cerevisiae, obtained from the crude ribosomal material, and containing the fatty acid synthetase, consisted primarily of 27S and 47S components. This...

EFFECTS OF ASPARTATE ON GROWTH AND ON THE SYNTHESIS OF α-AMYLASE IN PSEUDOMONAS SACCHAROPHILA

Eisenstadt, Jerome M., Klein, Harold P.

Eisenstadt, Jerome M. (Brandeis University, Waltham, Mass.), and Harold P. Klein. Effects of aspartate on growth and on the synthesis of α-amylase in Pseudomonas saccharophila. J. Bacteriol....

EVIDENCE FOR THE DE NOVO SYNTHESIS OF THE ALPHA-AMYLASE OF PSEUDOMONAS SACCHAROPHILA

Eisenstadt, Jerome M., Klein, Harold P.

Eisenstadt, Jerome M. (Brandeis University, Waltham, Mass.) and Harold P. Klein. Evidence for the de novo synthesis of the alpha-amylase of Pseudomonas saccharophila. J. Bacteriol. 82:798–807....

Variations in the Localization of Acetyl-Coenzyme A Synthetase in Aerobic Yeast Cells

Klein, Harold P., Jahnke, Linda

In cells of Saccharomyces cerevisiae growing aerobically for 24 hr, acetyl-coenzyme A synthetase [acetate: CoA ligase (AMP), EC 6.2.1.1] was localized principally in the microsomal fraction. On...

Membranes of Saccharomyces cerevisiae

Klein, Harold P., Volkmann, Carol, Weibel, Joseph

A crude small particle pellet, obtained from postmitochondrial supernatant fractions of Saccharomyces cerevisiae, contains about half the ergosterol and phospholipid of crude cell homogenates. Most...

Cellular Localization of Acetyl-Coenzyme A Synthetase in Yeast

Klein, Harold P., Jahnke, Linda

In cells of Saccharomyces cerevisiae grown with glucose in standing cultures, the microsomal fraction had the highest specific activity for acetyl-coenzyme A synthetase and contained the greatest...

Nature of Particles Involved in Lipid Synthesis in Yeast

Klein, Harold P.

Klein, Harold P. (Ames Research Center, Moffett Field, Calif.). Nature of particles involved in lipid synthesis in yeast. J. Bacteriol. 90:227–234. 1965.—Mitochondria-free homogenates of...

Effects of α-Glycerophosphate and of Palmityl-Coenzyme A on Lipid Synthesis in Yeast Extracts

White, David, Klein, Harold P.

White, David (Ames Research Center, Moffett Field, Calif.), and Harold P. Klein. Effects of α-glycerophosphate and of palmityl-coenzyme A on lipid synthesis in yeast extracts. J. Bacteriol....