Barbara Hobom-schnegg, Harriet L. Robinson, W. S. Robinson
When synchronized chicken embryo cells were infected with Rous sarcoma virus in the the G-I period of the cell cycle, virus replication was delayed by several hours compared with cells infected in...
Su, Jin, Luscher, Mark A., Xiong, Yelin, Rustam, Tarick, Amara, Rama Rao, Rakasz, Eva, ...
Simian immunodeficiency virus (SIV) infection of rhesus macaques provides an excellent model for investigating the basis of protective immunity against human immunodeficiency virus (HIV). One...
Su, Jin, Luscher, Mark A., Xiong, Yelin, Rustam, Tarick, Amara, Rama Rao, Rakasz, Eva, ...
Simian immunodeficiency virus (SIV) infection of rhesus macaques provides an excellent model for investigating the basis of protective immunity against human immunodeficiency virus (HIV). One...
Th1 genetic adjuvants modulate immune responses in neonates (2001)
Pertmer, Tamera Marie, Oran, Alp E., Madorin, Catherine A., Robinson, Harriet L.
In these studies, we address the ability of DNA encoding Th1 cytokines to bias the isotype of antibody raised by neonatal or adult immunization with an influenza hemagglutinin expressing DNA...
Pertmer, Tamera Marie, Oran, Alp E., Moser, Janice M., Madorin, Catherine A., Robinson, Harriet L.
Maternal antibody is the major form of protection from disease in early life when the neonatal immune system is still immature; however, the presence of maternal antibody also interferes with active...
Polack, Fernando P., Lee, Sok H., Permar, Sallie R., Manyara, Elizabeth, Nousari, Hossein G., Jeng, Yaikah, ...
Measles remains a principal cause of worldwide mortality, in part because young infants cannot be immunized effectively. Development of new vaccines has been hindered by previous experience with a...
Basic mechanisms of DNA-raised antibody responses to intramuscular and gene gun immunizations (2000)
Boyle, Christine Margaret, Robinson, Harriet L.
DNA-raised antibody (Ab) responses have been compared for the dependence on CD4+ and CD8+ cells, the longevity of functional antigen (Ag) expression, and the nature of the Ag-presenting cell after...
DNA immunization: effect of secretion of DNA-expressed hemagglutinins on antibody responses (1999)
Torres, Celia Aurora Tiglao, Yang, Kejian, Mustafa, Farah, Robinson, Harriet L.
DNA vaccines expressing plasma membrane and secreted forms of the influenza and measles virus hemagglutinins (HAs) have been used to evaluate the effect of secretion on DNA-raised antibody responses....
Feltquate, David Marc, Robinson, Harriet L.
We previously showed that intramuscular saline DNA immunizations favor the development of an IgG2a-dominant Th1 immune response, whereas gene gun DNA immunizations stimulate the production of an...
Pertmer, Tamera Marie, Robinson, Harriet L.
Neonatal mice have immature immune systems with defects in several components of inflammatory, innate, and specific immune responses and develop a preferential T helper type 2 response following...
Lu, Shan, Wyatt, Richard T., Richmond, Joan F. L., Mustafa, Farah, Wang, Shixia, Weng, Jiayu, ...
DNA vaccines that express the human immunodeficiency virus type 1 HXB-2 envelope glycoprotein (Env) with or without deletions of the major variable regions V1/V2 and V3 were tested for the ability to...
Robinson, Harriet L., Torres, Celia Aurora Tiglao
DNA vaccines use eukaryotic expression vectors to produce immunizing proteins in the vaccinated host. Popular methods of delivery are intramuscular and intradermal saline injections of DNA and gene...
Robinson, Harriet L., Boyle, Christine Margaret, Feltquate, David Marc, Morin, Merribeth J., Santoro, Joseph C., Webster, Robert G.
DNA-based immunizations have been used to analyze the ability of DNA-expressed hemagglutinin (HA) and nucleoprotein (NP) to protect BALB/c mice against a homologous influenza virus, A/PR/8/34 (H1N1),...
Iwasaki, Akiko, Torres, Celia Aurora Tiglao, Ohashi, Pamela S., Robinson, Harriet L., Barber, Brian H.
Although plasmid DNA immunization provides an effective means of inducing CTL responses to an expressed Ag, the mechanism by which CTL precursors are activated remains to be established. Insights...
Early studies on DNA-based immunizations for measles virus (1997)
Yang, Hyekyung, Mustafa, Farah, Valsamakis, Alexandra, Santoro, Joseph C., Griffin, Diane E., Robinson, Harriet L.
DNA-mediated immunizations have been used to raise neutralizing antibodies for measles virus. Single inoculations of plasmids expressing measles hemagglutinin or fusion glycoproteins raised...
Torres, Celia Aurora Tiglao, Iwasaki, Akiko, Barber, Brian H., Robinson, Harriet L.
Surgical ablations revealed different dependencies on the injection site tissue for i.m. needle and epidermal gene gun DNA immunizations. Excision of an injected muscle bundle within 10 min of DNA...
Richmond, Joan F. L., Mustafa, Farah, Lu, Shan, Santoro, Joseph C., Weng, Jiayu, O'Connell, Maryellen, ...
HIV-1 envelopes from two series of primary isolates (from Swedish patients 5 and 6), from JR-FL and BaL (prototypic monocyte/macrophage tropic viruses) and from HXB-2 (a prototypic...
Mustafa, Farah, Richmond, Joan F. L., Fernandez-Larsson, Roberto, Lu, Shan, Fredriksson, Robert, Fenyo, Eva Maria, ...
Seven envelope regions from two series of patient isolates have been molecularly cloned and analyzed for replication phenotypes and immunogenicity. Growth potential was analyzed for env sequences...
Feltquate, David Marc, Heaney, Shaun, Webster, Robert G., Robinson, Harriet L.
Several routes and methods of DNA immunization have been shown to generate Ab, Th cells, and CTL responses. However, few studies have directly compared the immune responses generated by different...
Boyle, Christine Margaret, Morin, Merribeth J., Webster, Robert G., Robinson, Harriet L.
Antibody responses in mice immunized by a single gene gun inoculation of plasmid expressing the influenza virus H1 hemagglutinin and in mice immunized by a sublethal H1 influenza virus infection have...
Simian immunodeficiency virus DNA vaccine trial in macaques (1996)
Lu, Shan, Arthos, James, Montefiori, David C., Yasutomi, Yasuhiro, Manson, Kelledy, Mustafa, Farah, ...
An experimental vaccine consisting of five DNA plasmids expressing different combinations and forms of simian immunodeficiency virus-macaque (SIVmac) proteins has been evaluated for the ability to...
Robinson, Harriet L., Lu, Shan, Feltquate, David Marc, Torres, Celia Aurora Tiglao, Richmond, Joann M., Boyle, Christine Margaret, ...
Yasutomi, Yasuhiro, Robinson, Harriet L., Lu, Shan, Mustafa, Farah, Lekutis, Christine, Arthos, James, ...
In view of the growing evidence that virus-specific cytotoxic T lymphocytes (CTL) play an important role in containing the early spread of human immunodeficiency virus type 1 (HIV-1) in infected...
Zarozinski, Christopher C., Fynan, Ellen F., Selin, Liisa K., Robinson, Harriet L., Welsh, Raymond M.
Anti-viral CTL were induced in vitro using a particle bombardment device or "gene-gun" to deliver plasmid DNA encoding the nucleoprotein of the lymphocytic choriomeningitis virus (LCMV). Using this...
Foster, Rosalinda Gram, Lian, Jane B., Stein, Gary S., Robinson, Harriet L.
Avian leukosis virus (ALV)-induced osteopetrosis is caused by the abnormal growth and differentiation of osteoblasts. To evaluate the role of infection in osteopetrosis induction, the replication of...
Foster, Rosalinda Gram, Robinson, Harriet L.
Avian leukosis virus (ALV)-induced osteopetrosis is characterized by the presence of unintegrated viral DNA is diseased bone. To learn more about this phenomenon, ALVs with high (Br21) and low...
DNA vaccines: protective immunizations by parenteral, mucosal, and gene-gun inoculations (1993)
Fynan, Ellen F., Webster, Robert G., Fuller, D H, Haynes, Joel R., Santoro, Joseph C., Robinson, Harriet L.
Plasmid DNAs expressing influenza virus hemagglutinin glycoproteins have been tested for their ability to raise protective immunity against lethal influenza challenges of the same subtype. In trials...
Mustafa, Farah, Robinson, Harriet L.
Two molecularly cloned viruses, human immunodeficiency virus type 1 (HIV-1)-NL4-3 (NL4-3) and HIV-1-HXB-2 (HXB-2), have been used to study the role of HIV-1 auxiliary genes in the establishment of...
Induction of renal adenocarcinoma by a nonmutated erbB oncogene (1993)
Taglienti, Cherie A., Banner, Barbara F., Davis, Roger J., Robinson, Harriet L.
Oncogenicity tests have revealed that a nonmutated erbB oncogene induces renal adenocarcinoma in addition to erythroblastosis. The erbB oncogene is a truncated form of the chicken epidermal growth...
5' avian leukosis virus sequences and osteopetrotic potential (1992)
Robinson, Harriet L., Foster, Rosalinda Gram, Blais, Bruce P., Reinsch, Sigrid S., Newstein, Michael C., Shank, Peter R.
Recombinants of Rous-associated virus-0 and Br21 have been used to localize 5' viral sequences that affect the osteopetrotic potential of avian leukosis viruses. Rous-associated virus-0 is a benign...
Characterization of an angiosarcoma-inducing mutation in the erbB oncogene (1992)
Robinson, Harriet L., Tracy, Sharon Elizabeth, Nair, Nandini, Taglienti, Cherie A., Gamett, Daniel C.
The erbB oncogenes of two transducing viruses that arose in chicken 5005 have been molecularly characterized. One of these viruses, AEV-5005, caused erythroblastosis. The other, AAV-5005, caused...
Nair, Nandini, Davis, Roger J., Robinson, Harriet L.
We have compared the protein tyrosine kinase activities of the chicken epidermal growth factor receptor (chEGFR) and three ErbB proteins to learn whether cancer-activating mutations affect the...
Theroux, Steven J., Taglienti, Cherie A., Nair, Nandini, Countaway, Janice L., Robinson, Harriet L., Davis, Roger J.
The erbB oncogene encodes an altered form of the epidermal growth factor (EGF) receptor that lacks the extracellular ligand binding domain. This oncogene is exclusively leukemogenic. However, an...
Lu, Shan, Putney, Scott D., Robinson, Harriet L.
The entry of human immunodeficiency virus type 1 into two T-cell lines has been analyzed to determine the relative time courses with which virus entry can be blocked (i) by washing, (ii) by adding a...
Fernandez-Larsson, Roberto, Srivastava, Kishore K., Lu, Shan, Robinson, Harriet L.
Isolates of human immunodeficiency virus type 1 (HIV-1) undergo many different rates of replication, with the time course of replication being determined by the host cell and the virus. Recently, we...
Srivastava, Kishore K., Fernandez-Larsson, Roberto, Zinkus, Donna M., Robinson, Harriet L.
Single-cycle infections have been used to study the human immunodeficiency virus type 1 (HIV-1) life cycle in CD4+ T-cell lines that differ in their permissiveness for infection. In single-cycle...
Robinson, Harriet L., Zinkus, Donna M.
Human immunodeficiency virus type 1 DNA synthesis was followed in a CD4+ line of T cells (C8166) grown in the presence or absence of a monoclonal antibody to CD4 that blocks infection By 48 h after...
Infrequent involvement of c-fos in avian leukosis virus-induced nephroblastoma (1990)
Collart, K. L., Aurigemma, Rosemarie E., Smith, Ralph E., Kawai, Sadaaki, Robinson, Harriet L.
To determine whether c-fos is involved in avian leukosis virus-induced nephroblastoma, 28 tumors from chickens were analyzed for novel fos fragments. DNA from 1 of 16 clonal outgrowths (in chicken...
Brown, David W., Robinson, Harriet L.
Adsorption and penetration of retroviruses into eucaryotic cells is mediated by retroviral envelope glycoproteins interacting with host receptors. Recombinant avian leukosis viruses (ALVs) differing...
Gamett, Daniel C., Tracy, Sharon Elizabeth, Robinson, Harriet L.
Eleven recently isolated erbB-transducing viruses as well as avian erythroblastosis virus (AEV)-R (ES4) and AEV-H have been characterized for the type of disease they cause, their ability to...
Induction of angiosarcoma by a c-erbB transducing virus (1985)
Tracy, Sharon Elizabeth, Woda, Bruce A., Robinson, Harriet L.
Recently, 12 new transductions of c-erbB have been identified in a series of Rous-associated virus type 1-induced erythroleukemias. During the passage of these new transducing viruses it has become...
Pertmer, Tamera M., Oran, Alp E., Moser, Janice M., Madorin, Catherine A., Robinson, Harriet L.
Maternal antibody is the major form of protection from disease in early life when the neonatal immune system is still immature; however, the presence of maternal antibody also interferes with active...
Montefiori, David C., Safrit, Jeffrey T., Lydy, Shari L., Barry, Ashley P., Bilska, Miroslawa, Vo, Ha T. T., ...
The ability to generate antibodies that cross-neutralize diverse primary isolates is an important goal for human immunodeficiency virus type 1 (HIV-1) vaccine development. Most of the candidate HIV-1...
Xiong, Yelin, Luscher, Mark A., Altman, John D., Hulsey, Michael, Robinson, Harriet L., Ostrowski, Mario, ...
A vigorous expansion of antigen-specific CD8+ T cells lacking apparent effector function was observed in a rhesus macaque acutely infected with the simian immunodeficiency virus (SIV) strain...
Amara, Rama Rao, Smith, James M., Staprans, Silvija I., Montefiori, David C., Villinger, Francois, Altman, John D., ...
Cellular immune responses against epitopes in conserved Gag and Pol sequences of human immunodeficiency virus type 1 have become popular targets for candidate AIDS vaccines. Recently, we used a...
Regulation of DNA-Raised Immune Responses by Cotransfected Interferon Regulatory Factors
Sasaki, Shin, Amara, Rama Rao, Yeow, Wen-Shuz, Pitha, Paula M., Robinson, Harriet L.
Interferon regulatory factor 1 (IRF-1), IRF-3, and IRF-7 have been tested as genetic adjuvants for influenza virus hemagglutinin (HA) and nucleoprotein vaccine DNAs. Cotransfection of HA with IRF-3...
Amara, Rama Rao, Villinger, Francois, Staprans, Silvija I., Altman, John D., Montefiori, David C., Kozyr, Natalia L., ...
Recently we demonstrated the control of a mucosal challenge with a pathogenic chimera of simian and human immunodeficiency virus (SHIV-89.6P) by priming with a Gag-Pol-Env-expressing DNA and boosting...
Tang, Yuyang, Villinger, Francois, Staprans, Silvija I., Amara, Rama Rao, Smith, James M., Herndon, James G., ...
In a recent vaccine trial, we showed efficient control of a virulent simian-human immunodeficiency virus SHIV-89.6P challenge by priming with a Gag-Pol-Env-expressing DNA and boosting with a...
Neuman De Vegvar, Henry E., Amara, Rama Rao, Steinman, Lawrence, Utz, Paul J., Robinson, Harriet L., Robinson, William H.
We developed antigen microarrays to profile the breadth, strength, and kinetics of epitope-specific antiviral antibody responses in vaccine trials with a simian-human immunodeficiency virus (SHIV)...
Conklin, Kathleen F., Coffin, John M., Robinson, Harriet L., Groudine, Mark, Eisenman, Robert
The endogenous avian provirus ev-1 is widespread in white leghorn chickens. Although it has no major structural defects, ev-1 has not been associated with any phenotype and is ordinarily expressed at...
Oran, Alp E., Robinson, Harriet L.
For this study, we used DNA-based immunizations to elicit gamma interferon-producing (Tc1) or interleukin 4 (IL-4)-producing (Tc2) CD8 T cells to the influenza virus nucleoprotein. We examined the...
Proteins of Avian Tumor Viruses with Different Coat Antigens
Robinson, William S., Hung, Paul, Robinson, Harriet L., Ralph, David D.
Isoelectric focusing of avian tumor viruses with distinct type-specific envelope antigens demonstrated no differences in isoelectric points. Viruses with different type-specific antigens were found...
DNA in Uninfected and Virus-Infected Cells Complementary to Avian Tumor Virus RNA
Rosenthal, Peter N., Robinson, Harriet L., Robinson, William S., Hanafusa, Teruko, Hanafusa, Hidesaburo
The 70S RNA component of several avian tumor viruses was hybridized with DNA extracted from avian tumor virus-infected and uninfected chicken and Japanese quail cells. Tritium-labeled 70S RNAs from...
Sadagopal, Shanmugalakshmi, Amara, Rama Rao, Montefiori, David C., Wyatt, Linda S., Staprans, Silvija I., Kozyr, Natalia L., ...
In 2001, we reported 20 weeks of control of challenge with the virulent 89.6P chimera of simian and human immunodeficiency viruses (SHIV-89.6P) by a Gag-Pol-Env vaccine consisting of DNA priming and...
Amara, Rama Rao, Patel, Kalpana, Niedziela, Genevieve, Nigam, Pragati, Sharma, Sunita, Staprans, Silvija I., ...
Among the most effective vaccine candidates tested in the simian immunodeficiency virus (SIV)/macaque system, live attenuated viruses have been shown to provide the best protection from challenge. To...
C3d enhancement of neutralizing antibodies to measles hemagglutinin
Green, Thomas D., Newton, Bruce R., Rota, Paul A., Xu, Yan, Robinson, Harriet L., Ross, Ted M.
Measles remains a major cause of worldwide infant mortality despite the use of current live attenuated vaccines. New approaches to measles virus (MV) vaccine development are critical to interrupt the...
Pertmer, Tamera M., Oran, Alp E., Moser, Janice M., Madorin, Catherine A., Robinson, Harriet L.
Maternal antibody is the major form of protection from disease in early life when the neonatal immune system is still immature; however, the presence of maternal antibody also interferes with active...
Montefiori, David C., Safrit, Jeffrey T., Lydy, Shari L., Barry, Ashley P., Bilska, Miroslawa, Vo, Ha T. T., ...
The ability to generate antibodies that cross-neutralize diverse primary isolates is an important goal for human immunodeficiency virus type 1 (HIV-1) vaccine development. Most of the candidate HIV-1...
Xiong, Yelin, Luscher, Mark A., Altman, John D., Hulsey, Michael, Robinson, Harriet L., Ostrowski, Mario, ...
A vigorous expansion of antigen-specific CD8+ T cells lacking apparent effector function was observed in a rhesus macaque acutely infected with the simian immunodeficiency virus (SIV) strain...
Amara, Rama Rao, Smith, James M., Staprans, Silvija I., Montefiori, David C., Villinger, Francois, Altman, John D., ...
Cellular immune responses against epitopes in conserved Gag and Pol sequences of human immunodeficiency virus type 1 have become popular targets for candidate AIDS vaccines. Recently, we used a...
Regulation of DNA-Raised Immune Responses by Cotransfected Interferon Regulatory Factors
Sasaki, Shin, Amara, Rama Rao, Yeow, Wen-Shuz, Pitha, Paula M., Robinson, Harriet L.
Interferon regulatory factor 1 (IRF-1), IRF-3, and IRF-7 have been tested as genetic adjuvants for influenza virus hemagglutinin (HA) and nucleoprotein vaccine DNAs. Cotransfection of HA with IRF-3...
Amara, Rama Rao, Villinger, Francois, Staprans, Silvija I., Altman, John D., Montefiori, David C., Kozyr, Natalia L., ...
Recently we demonstrated the control of a mucosal challenge with a pathogenic chimera of simian and human immunodeficiency virus (SHIV-89.6P) by priming with a Gag-Pol-Env-expressing DNA and boosting...
Tang, Yuyang, Villinger, Francois, Staprans, Silvija I., Amara, Rama Rao, Smith, James M., Herndon, James G., ...
In a recent vaccine trial, we showed efficient control of a virulent simian-human immunodeficiency virus SHIV-89.6P challenge by priming with a Gag-Pol-Env-expressing DNA and boosting with a...
Neuman De Vegvar, Henry E., Amara, Rama Rao, Steinman, Lawrence, Utz, Paul J., Robinson, Harriet L., Robinson, William H.
We developed antigen microarrays to profile the breadth, strength, and kinetics of epitope-specific antiviral antibody responses in vaccine trials with a simian-human immunodeficiency virus (SHIV)...
Conklin, Kathleen F., Coffin, John M., Robinson, Harriet L., Groudine, Mark, Eisenman, Robert
The endogenous avian provirus ev-1 is widespread in white leghorn chickens. Although it has no major structural defects, ev-1 has not been associated with any phenotype and is ordinarily expressed at...
Oran, Alp E., Robinson, Harriet L.
For this study, we used DNA-based immunizations to elicit gamma interferon-producing (Tc1) or interleukin 4 (IL-4)-producing (Tc2) CD8 T cells to the influenza virus nucleoprotein. We examined the...
Proteins of Avian Tumor Viruses with Different Coat Antigens
Robinson, William S., Hung, Paul, Robinson, Harriet L., Ralph, David D.
Isoelectric focusing of avian tumor viruses with distinct type-specific envelope antigens demonstrated no differences in isoelectric points. Viruses with different type-specific antigens were found...
DNA in Uninfected and Virus-Infected Cells Complementary to Avian Tumor Virus RNA
Rosenthal, Peter N., Robinson, Harriet L., Robinson, William S., Hanafusa, Teruko, Hanafusa, Hidesaburo
The 70S RNA component of several avian tumor viruses was hybridized with DNA extracted from avian tumor virus-infected and uninfected chicken and Japanese quail cells. Tritium-labeled 70S RNAs from...
Sadagopal, Shanmugalakshmi, Amara, Rama Rao, Montefiori, David C., Wyatt, Linda S., Staprans, Silvija I., Kozyr, Natalia L., ...
In 2001, we reported 20 weeks of control of challenge with the virulent 89.6P chimera of simian and human immunodeficiency viruses (SHIV-89.6P) by a Gag-Pol-Env vaccine consisting of DNA priming and...
Amara, Rama Rao, Patel, Kalpana, Niedziela, Genevieve, Nigam, Pragati, Sharma, Sunita, Staprans, Silvija I., ...
Among the most effective vaccine candidates tested in the simian immunodeficiency virus (SIV)/macaque system, live attenuated viruses have been shown to provide the best protection from challenge. To...
Kannanganat, Sunil, Ibegbu, Chris, Chennareddi, Lakshmi, Robinson, Harriet L., Amara, Rama Rao
Virus-specific CD4 T cells are endowed with multiple functions, such as cytokine production, CD40 ligand (CD40L) expression (associated with the costimulation of CD8 and B cells), and degranulation...
Kannanganat, Sunil, Kapogiannis, Bill G., Ibegbu, Chris, Chennareddi, Lakshmi, Goepfert, Paul, Robinson, Harriet L., ...
Here, we evaluate the cytokine coexpression profiles of human immunodeficiency virus (HIV)-specific CD4 T cells for the expression of the cytokines gamma interferon (IFN-γ), interleukin-2, and tumor...
Kwissa, Marcin, Amara, Rama R., Robinson, Harriet L., Moss, Bernard, Alkan, Sefik, Jabbar, Abdul, ...
DNA vaccines offer promising strategies for immunization against infections. However, their clinical use requires improvements in immunogenicity. We explored the efficacy of Toll-like receptor (TLR)...
Sadagopal, Shanmugalakshmi, Amara, Rama Rao, Kannanganat, Sunil, Sharma, Sunita, Chennareddi, Lakshmi, Robinson, Harriet L.
In this study, we monitored the temporal breadths, frequencies, and functions of antiviral CD4 and CD8 T cells in 2 of 22 DNA/modified vaccinia virus Ankara-vaccinated macaques that lost control of a...
Zhao, Jun, Lai, Lilin, Amara, Rama Rao, Montefiori, David C., Villinger, Francois, Chennareddi, Lakshmi, ...
A major challenge for human immunodeficiency virus (HIV)/AIDS vaccines is the elicitation of anti-Env antibodies (Ab) capable of neutralizing the diversity of isolates in the pandemic. Here, we show...