In vivo behavior of a Helicobacter pylori SS1 nixA mutant with reduced urease activity (2002)
Nolan, Kylie J., McGee, David J., Mitchell, Hazel M., Kolesnikow, Tassia, Harro, Janette M., O'Rourke, Jani, ...
Helicobacter pylori mutants devoid of urease activity fail to colonize the gastric mucosa of mice; however, the effect of decreased levels of urease on colonization has not been examined. The nixA...
In vivo behavior of a Helicobacter pylori SS1 nixA mutant with reduced urease activity (2002)
Nolan, Kylie J., McGee, David J., Mitchell, Hazel M., Kolesnikow, Tassia, Harro, Janette M., O'Rourke, Jani, ...
Helicobacter pylori mutants devoid of urease activity fail to colonize the gastric mucosa of mice; however, the effect of decreased levels of urease on colonization has not been examined. The nixA...
In vivo behavior of a Helicobacter pylori SS1 nixA mutant with reduced urease activity (2002)
Nolan, Kylie J., McGee, David J., Mitchell, Hazel M., Kolesnikow, Tassia, Harro, Janette M., O'Rourke, Jani, ...
Helicobacter pylori mutants devoid of urease activity fail to colonize the gastric mucosa of mice; however, the effect of decreased levels of urease on colonization has not been examined. The nixA...
In vivo behavior of a Helicobacter pylori SS1 nixA mutant with reduced urease activity (2002)
Nolan, Kylie J., McGee, David J., Mitchell, Hazel M., Kolesnikow, Tassia, Harro, Janette M., O'Rourke, Jani, ...
Helicobacter pylori mutants devoid of urease activity fail to colonize the gastric mucosa of mice; however, the effect of decreased levels of urease on colonization has not been examined. The nixA...
In vivo behavior of a Helicobacter pylori SS1 nixA mutant with reduced urease activity (2002)
Nolan, Kylie J., McGee, David J., Mitchell, Hazel M., Kolesnikow, Tassia, Harro, Janette M., O'Rourke, Jani, ...
Helicobacter pylori mutants devoid of urease activity fail to colonize the gastric mucosa of mice; however, the effect of decreased levels of urease on colonization has not been examined. The nixA...
In vivo behavior of a Helicobacter pylori SS1 nixA mutant with reduced urease activity (2002)
Nolan, Kylie J., McGee, David J., Mitchell, Hazel M., Kolesnikow, Tassia, Harro, Janette M., O'Rourke, Jani, ...
Helicobacter pylori mutants devoid of urease activity fail to colonize the gastric mucosa of mice; however, the effect of decreased levels of urease on colonization has not been examined. The nixA...
In vivo behavior of a Helicobacter pylori SS1 nixA mutant with reduced urease activity (2002)
Nolan, Kylie J., McGee, David J., Mitchell, Hazel M., Kolesnikow, Tassia, Harro, Janette M., O'Rourke, Jani, ...
Helicobacter pylori mutants devoid of urease activity fail to colonize the gastric mucosa of mice; however, the effect of decreased levels of urease on colonization has not been examined. The nixA...
In vivo behavior of a Helicobacter pylori SS1 nixA mutant with reduced urease activity (2002)
Nolan, Kylie J., McGee, David J., Mitchell, Hazel M., Kolesnikow, Tassia, Harro, Janette M., O'Rourke, Jani, ...
Helicobacter pylori mutants devoid of urease activity fail to colonize the gastric mucosa of mice; however, the effect of decreased levels of urease on colonization has not been examined. The nixA...
In vivo behavior of a Helicobacter pylori SS1 nixA mutant with reduced urease activity (2002)
Nolan, Kylie J., McGee, David J., Mitchell, Hazel M., Kolesnikow, Tassia, Harro, Janette M., O'Rourke, Jani, ...
Helicobacter pylori mutants devoid of urease activity fail to colonize the gastric mucosa of mice; however, the effect of decreased levels of urease on colonization has not been examined. The nixA...
Gobert, Alain P., McGee, David J., Akhtar, Mahmood, Mendz, George L., Newton, Jamie C., Cheng, Yulan, ...
The antimicrobial effect of nitric oxide (NO) is an essential part of innate immunity. The vigorous host response to the human gastric pathogen Helicobacter pylori fails to eradicate the organism,...
Testerman, Traci L., McGee, David J., Mobley, Harry L. T.
Obstacles continue to hinder in vitro studies of the gastric human pathogen Helicobacter pylori, including difficulty culturing the organism in the absence of serum or blood, rapid loss of viability...
Isolation of Helicobacter pylori Genes That Modulate Urease Activity
McGee, David J., May, Carrie A., Garner, Rachel M., Himpsl, Janette M., Mobley, Harry L. T.
Helicobacter pylori urease, a nickel-requiring metalloenzyme, hydrolyzes urea to NH3 and CO2. We sought to identify H. pylori genes that modulate urease activity by constructing pHP8080, a plasmid...
Fulkerson, John F., Mobley, Harry L. T.
NixA, the high-affinity cytoplasmic membrane nickel transport protein of Helicobacter pylori, imports Ni2+ into the cell for insertion into the active site of the urease metalloenzyme, which is...
Heimer, Susan R., Mobley, Harry L. T.
Proteus mirabilis, a gram-negative bacterium associated with complicated urinary tract infections, produces a metalloenzyme urease which hydrolyzes urea to ammonia and carbon dioxide. The apourease...
Poore, Carrie A., Coker, Christopher, Dattelbaum, Jonathan D., Mobley, Harry L. T.
Proteus mirabilis urease catalyzes the hydrolysis of urea to CO2 and NH3, resulting in urinary stone formation in individuals with complicated urinary tract infections. UreR, a member of the AraC...
Li, Xin, Johnson, David E., Mobley, Harry L. T.
Two new genes, mrpH and mrpJ, were identified downstream of mrpG in the mrp gene cluster encoding mannose-resistant Proteus-like (MR/P) fimbriae of uropathogenic Proteus mirabilis. Since the...
Johnson, David E., Bahrani, Farah K., Lockatell, C. Virginia, Drachenberg, Cinthia B., Hebel, J. Richard, Belas, Robert, ...
We tested the hypothesis that experimental Proteus mirabilis urinary tract infection in mice would protect against homologous bladder rechallenge. Despite production of serum immunoglobulin G (IgG)...
Gunther, Nereus W., Lockatell, Virginia, Johnson, David E., Mobley, Harry L. T.
Escherichia coli is the primary cause of uncomplicated infections of the urinary tract including cystitis. More serious infections, characterized as acute pyelonephritis, can also develop. Type 1...
Cloning, Expression, and Catalytic Activity of Helicobacter hepaticus Urease
Beckwith, Catherine S., McGee, David J., Mobley, Harry L. T., Riley, Lela K.
Helicobacter hepaticus causes disease in the liver and lower intestinal tract of mice. It is strongly urease positive, although it does not live in an acidic environment. The H. hepaticus urease gene...
McGee, David J., Radcliff, Fiona J., Mendz, George L., Ferrero, Richard L., Mobley, Harry L. T.
Arginase of the Helicobacter pylori urea cycle hydrolyzes l-arginine to l-ornithine and urea. H. pylori urease hydrolyzes urea to carbon dioxide and ammonium, which neutralizes acid. Both enzymes are...
Shen, Zeli, Schauer, David B., Mobley, Harry L. T., Fox, James G.
Infection with Helicobacter hepaticus causes chronic active hepatitis in certain strains of mice and is associated with hepatocellular carcinoma in A/JCr mice. Like the gastric helicobacters, H....
Zhao, Hui, Thompson, Richard B., Lockatell, Virginia, Johnson, David E., Mobley, Harry L. T.
Proteus mirabilis, a cause of complicated urinary tract infection, expresses urease when exposed to urea. While it is recognized that the positive transcriptional activator UreR induces gene...
Insertional mutagenesis studies of mrpB, a putative pilin-encoding open reading frame of the mrp gene cluster, which encodes mannose-resistant Proteus-like (MR/P) fimbriae of Proteus mirabilis,...
Garner, Rachel M., Fulkerson, John, Mobley, Harry L. T.
Helicobacter pylori urease, produced in abundance, is indispensable for the survival of H. pylori in animal hosts. Urea is hydrolyzed by the enzyme, resulting in the liberation of excess ammonia,...
Lim, Jean K., Gunther, Nereus W., Zhao, Hui, Johnson, David E., Keay, Susan K., Mobley, Harry L. T.
Type 1 fimbriae, expressed by most Escherichia coli strains, are thought to attach to human uroepithelium as an initial step in the pathogenesis of urinary tract infections (UTI). Numerous reports...
Guyer, Debra M., Kao, Jyh-Shyang, Mobley, Harry L. T.
Urinary tract infection is the most frequently diagnosed kidney and urologic disease and Escherichia coli is by far the most common etiologic agent. Uropathogenic strains have been shown to contain...
Helicobacter pylori Containing Only Cytoplasmic Urease Is Susceptible to Acid
Krishnamurthy, Partha, Parlow, Mary, Zitzer, Jason B., Vakil, Nimish B., Mobley, Harry L. T., Levy, Marilyn, ...
Helicobacter pylori, an important etiologic agent in a variety of gastroduodenal diseases, produces large amounts of urease as an essential colonization factor. We have demonstrated previously that...
H-NS Is a Repressor of the Proteus mirabilis Urease Transcriptional Activator Gene ureR
Coker, Christopher, Bakare, Olubunmi O., Mobley, Harry L. T.
Expression of Proteus mirabilis urease is governed by UreR, an AraC-like positive transcriptional activator. A poly(A) tract nucleotide sequence, consisting of A6TA2CA2TGGTA5GA6TGA5, is located 16 bp...
Repression of bacterial motility by a novel fimbrial gene product
Li, Xin, Rasko, David A., Lockatell, C.Virginia, Johnson, David E., Mobley, Harry L.T.
Proteus mirabilis is a common uropathogen in patients with long-term catheterization or with structural or functional abnormalities in the urinary tract. The mannose-resistant, Proteus-like (MR/P)...
Li, Xin, Zhao, Hui, Lockatell, C. Virginia, Drachenberg, Cinthia B., Johnson, David E., Mobley, Harry L. T.
The virulence of a urease-negative mutant of uropathogenic Proteus mirabilis and its wild-type parent strain was assessed by using a CBA mouse model of catheterized urinary tract infection. Overall,...
In Vivo Behavior of a Helicobacter pylori SS1 nixA Mutant with Reduced Urease Activity
Nolan, Kylie J., McGee, David J., Mitchell, Hazel M., Kolesnikow, Tassia, Harro, Janette M., O'Rourke, Jani, ...
Helicobacter pylori mutants devoid of urease activity fail to colonize the gastric mucosa of mice; however, the effect of decreased levels of urease on colonization has not been examined. The nixA...
Heimer, Susan R., Welch, Rod A., Perna, Nicole T., Pósfai, György, Evans, Peter S., Kaper, James B., ...
Recent genomic analyses of Escherichia coli O157:H7 strain EDL933 revealed two loci encoding urease gene homologues (ureDABCEFG), which are absent in nonpathogenic E. coli strain K-12. This report...
Gunther, Nereus W., Snyder, Jennifer A., Lockatell, Virginia, Blomfield, Ian, Johnson, David E., Mobley, Harry L. T.
Type 1 fimbria is a proven virulence factor of uropathogenic Escherichia coli (UPEC), causing urinary tract infections. Expression of the fimbria is regulated at the transcriptional level by a...
Guyer, Debra M., Radulovic, Suzana, Jones, Faye-Ellen, Mobley, Harry L. T.
The secreted autotransporter toxin (Sat) of uropathogenic Escherichia coli exhibits cytopathic activity upon incubation with HEp-2 cells. We further investigated the effects of Sat on cell lines more...
Dattelbaum, Jonathan D., Lockatell, C. Virginia, Johnson, David E., Mobley, Harry L. T.
Proteus mirabilis, a cause of complicated urinary tract infection, produces urease, an essential virulence factor for this species. UreR, a member of the AraC/XylS family of transcriptional...
Jansen, Angela M., Lockatell, C. Virginia, Johnson, David E., Mobley, Harry L. T.
Proteus mirabilis, a common cause of nosocomial and catheter-associated urinary tract infection, colonizes the bladder and ascends the ureters to the proximal tubules of the kidneys, leading to the...
Development of an Intranasal Vaccine To Prevent Urinary Tract Infection by Proteus mirabilis
Li, Xin, Lockatell, C. Virginia, Johnson, David E., Lane, M. Chelsea, Warren, John W., Mobley, Harry L. T.
Proteus mirabilis commonly infects the complicated urinary tract and is associated with urolithiasis. Stone formation is caused by bacterial urease, which hydrolyzes urea to ammonia, causing local pH...
Heimer, Susan R., Rasko, David A., Lockatell, C. Virginia, Johnson, David E., Mobley, Harry L. T.
We have identified two chromosomal open reading frames in uropathogenic Escherichia coli (UPEC) strain CFT073 which are highly homologous to serine protease autotransporters Pic and Tsh. Both cloned...
Burall, Laurel S., Harro, Janette M., Li, Xin, Lockatell, C.Virginia, Himpsl, Stephanie D., Hebel, J. Richard, ...
Proteus mirabilis, a common cause of urinary tract infections (UTI) in individuals with functional or structural abnormalities or with long-term catheterization, forms bladder and kidney stones as a...
Transcriptome of Uropathogenic Escherichia coli during Urinary Tract Infection
Snyder, Jennifer A., Haugen, Brian J., Buckles, Eric L., Lockatell, C. Virginia, Johnson, David E., Donnenberg, Michael S., ...
A uropathogenic Escherichia coli strain CFT073-specific DNA microarray that includes each open reading frame was used to analyze the transcriptome of CFT073 bacteria isolated directly from the urine...
Jansen, Angela M., Lockatell, Virginia, Johnson, David E., Mobley, Harry L. T.
Proteus mirabilis, an etiologic agent of complicated urinary tract infections, expresses mannose-resistant Proteus-like (MR/P) fimbriae whose expression is phase variable. Here we examine the role of...
Li, Xin, Erbe, Jarrod L., Lockatell, C. Virginia, Johnson, David E., Jobling, Michael G., Holmes, Randall K., ...
This is a follow-up to our previous study using an intranasal vaccine composed of MrpH, the tip adhesin of the MR/P fimbria, and cholera toxin to prevent urinary tract infection by Proteus mirabilis...
Johnson, James R., Jelacic, Srdjan, Schoening, Laura M., Clabots, Connie, Shaikh, Nurmohammad, Mobley, Harry L. T., ...
The role of the Escherichia coli iron-regulated gene homologue adhesin (Iha) in the pathogenesis of urinary tract infections (UTIs) is unknown. We performed a series of complementary analyses to...
Role of Motility in the Colonization of Uropathogenic Escherichia coli in the Urinary Tract
Lane, M. Chelsea, Lockatell, Virginia, Monterosso, Greta, Lamphier, Daniel, Weinert, Julia, Hebel, J. Richard, ...
Uropathogenic Escherichia coli (UPEC) causes most uncomplicated urinary tract infections (UTIs) in humans. Flagellum-mediated motility and chemotaxis have been suggested to contribute to virulence by...
Coordinate Expression of Fimbriae in Uropathogenic Escherichia coli
Snyder, Jennifer A., Haugen, Brian J., Lockatell, C. Virginia, Maroncle, Nathalie, Hagan, Erin C., Johnson, David E., ...
Uropathogenic Escherichia coli is the most common etiological agent of urinary tract infections. Bacteria can often express multiple adhesins during infection in order to favor attachment to specific...
Snyder, Jennifer A., Lloyd, Amanda L., Lockatell, C. Virginia, Johnson, David E., Mobley, Harry L. T.
Type 1 fimbrial phase-locked mutants of uropathogenic Escherichia coli cystitis isolate F11 were used to assess the role of the invertible element during urinary tract infection. Compared to the wild...
Testerman, Traci L., Conn, P. Brian, Mobley, Harry L. T., McGee, David J.
The growth of the gastric pathogen Helicobacter pylori in the absence of serum remains challenging, and nutritional requirements have only partially been defined, while almost nothing is known about...
Lane, M. Chelsea, Lloyd, Amanda L., Markyvech, Tiffany A., Hagan, Erin C., Mobley, Harry L. T.
The prevalence and function of four chemoreceptors, Tsr, Tar, Trg, and Tap, were determined for a collection of uropathogenic, fecal-commensal, and diarrheagenic Escherichia coli strains. tar and tsr...
Gobert, Alain P., McGee, David J., Akhtar, Mahmood, Mendz, George L., Newton, Jamie C., Cheng, Yulan, ...
The antimicrobial effect of nitric oxide (NO) is an essential part of innate immunity. The vigorous host response to the human gastric pathogen Helicobacter pylori fails to eradicate the organism,...
Testerman, Traci L., McGee, David J., Mobley, Harry L. T.
Obstacles continue to hinder in vitro studies of the gastric human pathogen Helicobacter pylori, including difficulty culturing the organism in the absence of serum or blood, rapid loss of viability...
Isolation of Helicobacter pylori Genes That Modulate Urease Activity
McGee, David J., May, Carrie A., Garner, Rachel M., Himpsl, Janette M., Mobley, Harry L. T.
Helicobacter pylori urease, a nickel-requiring metalloenzyme, hydrolyzes urea to NH3 and CO2. We sought to identify H. pylori genes that modulate urease activity by constructing pHP8080, a plasmid...
Fulkerson, John F., Mobley, Harry L. T.
NixA, the high-affinity cytoplasmic membrane nickel transport protein of Helicobacter pylori, imports Ni2+ into the cell for insertion into the active site of the urease metalloenzyme, which is...
Heimer, Susan R., Mobley, Harry L. T.
Proteus mirabilis, a gram-negative bacterium associated with complicated urinary tract infections, produces a metalloenzyme urease which hydrolyzes urea to ammonia and carbon dioxide. The apourease...
Poore, Carrie A., Coker, Christopher, Dattelbaum, Jonathan D., Mobley, Harry L. T.
Proteus mirabilis urease catalyzes the hydrolysis of urea to CO2 and NH3, resulting in urinary stone formation in individuals with complicated urinary tract infections. UreR, a member of the AraC...
Li, Xin, Johnson, David E., Mobley, Harry L. T.
Two new genes, mrpH and mrpJ, were identified downstream of mrpG in the mrp gene cluster encoding mannose-resistant Proteus-like (MR/P) fimbriae of uropathogenic Proteus mirabilis. Since the...
Johnson, David E., Bahrani, Farah K., Lockatell, C. Virginia, Drachenberg, Cinthia B., Hebel, J. Richard, Belas, Robert, ...
We tested the hypothesis that experimental Proteus mirabilis urinary tract infection in mice would protect against homologous bladder rechallenge. Despite production of serum immunoglobulin G (IgG)...
Gunther, Nereus W., Lockatell, Virginia, Johnson, David E., Mobley, Harry L. T.
Escherichia coli is the primary cause of uncomplicated infections of the urinary tract including cystitis. More serious infections, characterized as acute pyelonephritis, can also develop. Type 1...
Cloning, Expression, and Catalytic Activity of Helicobacter hepaticus Urease
Beckwith, Catherine S., McGee, David J., Mobley, Harry L. T., Riley, Lela K.
Helicobacter hepaticus causes disease in the liver and lower intestinal tract of mice. It is strongly urease positive, although it does not live in an acidic environment. The H. hepaticus urease gene...
McGee, David J., Radcliff, Fiona J., Mendz, George L., Ferrero, Richard L., Mobley, Harry L. T.
Arginase of the Helicobacter pylori urea cycle hydrolyzes l-arginine to l-ornithine and urea. H. pylori urease hydrolyzes urea to carbon dioxide and ammonium, which neutralizes acid. Both enzymes are...
Shen, Zeli, Schauer, David B., Mobley, Harry L. T., Fox, James G.
Infection with Helicobacter hepaticus causes chronic active hepatitis in certain strains of mice and is associated with hepatocellular carcinoma in A/JCr mice. Like the gastric helicobacters, H....
Zhao, Hui, Thompson, Richard B., Lockatell, Virginia, Johnson, David E., Mobley, Harry L. T.
Proteus mirabilis, a cause of complicated urinary tract infection, expresses urease when exposed to urea. While it is recognized that the positive transcriptional activator UreR induces gene...
Insertional mutagenesis studies of mrpB, a putative pilin-encoding open reading frame of the mrp gene cluster, which encodes mannose-resistant Proteus-like (MR/P) fimbriae of Proteus mirabilis,...
Garner, Rachel M., Fulkerson, John, Mobley, Harry L. T.
Helicobacter pylori urease, produced in abundance, is indispensable for the survival of H. pylori in animal hosts. Urea is hydrolyzed by the enzyme, resulting in the liberation of excess ammonia,...
Lim, Jean K., Gunther, Nereus W., Zhao, Hui, Johnson, David E., Keay, Susan K., Mobley, Harry L. T.
Type 1 fimbriae, expressed by most Escherichia coli strains, are thought to attach to human uroepithelium as an initial step in the pathogenesis of urinary tract infections (UTI). Numerous reports...
Guyer, Debra M., Kao, Jyh-Shyang, Mobley, Harry L. T.
Urinary tract infection is the most frequently diagnosed kidney and urologic disease and Escherichia coli is by far the most common etiologic agent. Uropathogenic strains have been shown to contain...
Helicobacter pylori Containing Only Cytoplasmic Urease Is Susceptible to Acid
Krishnamurthy, Partha, Parlow, Mary, Zitzer, Jason B., Vakil, Nimish B., Mobley, Harry L. T., Levy, Marilyn, ...
Helicobacter pylori, an important etiologic agent in a variety of gastroduodenal diseases, produces large amounts of urease as an essential colonization factor. We have demonstrated previously that...
H-NS Is a Repressor of the Proteus mirabilis Urease Transcriptional Activator Gene ureR
Coker, Christopher, Bakare, Olubunmi O., Mobley, Harry L. T.
Expression of Proteus mirabilis urease is governed by UreR, an AraC-like positive transcriptional activator. A poly(A) tract nucleotide sequence, consisting of A6TA2CA2TGGTA5GA6TGA5, is located 16 bp...
Repression of bacterial motility by a novel fimbrial gene product
Li, Xin, Rasko, David A., Lockatell, C.Virginia, Johnson, David E., Mobley, Harry L.T.
Proteus mirabilis is a common uropathogen in patients with long-term catheterization or with structural or functional abnormalities in the urinary tract. The mannose-resistant, Proteus-like (MR/P)...
Li, Xin, Zhao, Hui, Lockatell, C. Virginia, Drachenberg, Cinthia B., Johnson, David E., Mobley, Harry L. T.
The virulence of a urease-negative mutant of uropathogenic Proteus mirabilis and its wild-type parent strain was assessed by using a CBA mouse model of catheterized urinary tract infection. Overall,...
In Vivo Behavior of a Helicobacter pylori SS1 nixA Mutant with Reduced Urease Activity
Nolan, Kylie J., McGee, David J., Mitchell, Hazel M., Kolesnikow, Tassia, Harro, Janette M., O'Rourke, Jani, ...
Helicobacter pylori mutants devoid of urease activity fail to colonize the gastric mucosa of mice; however, the effect of decreased levels of urease on colonization has not been examined. The nixA...
Heimer, Susan R., Welch, Rod A., Perna, Nicole T., Pósfai, György, Evans, Peter S., Kaper, James B., ...
Recent genomic analyses of Escherichia coli O157:H7 strain EDL933 revealed two loci encoding urease gene homologues (ureDABCEFG), which are absent in nonpathogenic E. coli strain K-12. This report...
Gunther, Nereus W., Snyder, Jennifer A., Lockatell, Virginia, Blomfield, Ian, Johnson, David E., Mobley, Harry L. T.
Type 1 fimbria is a proven virulence factor of uropathogenic Escherichia coli (UPEC), causing urinary tract infections. Expression of the fimbria is regulated at the transcriptional level by a...
Guyer, Debra M., Radulovic, Suzana, Jones, Faye-Ellen, Mobley, Harry L. T.
The secreted autotransporter toxin (Sat) of uropathogenic Escherichia coli exhibits cytopathic activity upon incubation with HEp-2 cells. We further investigated the effects of Sat on cell lines more...
Dattelbaum, Jonathan D., Lockatell, C. Virginia, Johnson, David E., Mobley, Harry L. T.
Proteus mirabilis, a cause of complicated urinary tract infection, produces urease, an essential virulence factor for this species. UreR, a member of the AraC/XylS family of transcriptional...
Jansen, Angela M., Lockatell, C. Virginia, Johnson, David E., Mobley, Harry L. T.
Proteus mirabilis, a common cause of nosocomial and catheter-associated urinary tract infection, colonizes the bladder and ascends the ureters to the proximal tubules of the kidneys, leading to the...
Development of an Intranasal Vaccine To Prevent Urinary Tract Infection by Proteus mirabilis
Li, Xin, Lockatell, C. Virginia, Johnson, David E., Lane, M. Chelsea, Warren, John W., Mobley, Harry L. T.
Proteus mirabilis commonly infects the complicated urinary tract and is associated with urolithiasis. Stone formation is caused by bacterial urease, which hydrolyzes urea to ammonia, causing local pH...
Heimer, Susan R., Rasko, David A., Lockatell, C. Virginia, Johnson, David E., Mobley, Harry L. T.
We have identified two chromosomal open reading frames in uropathogenic Escherichia coli (UPEC) strain CFT073 which are highly homologous to serine protease autotransporters Pic and Tsh. Both cloned...
Burall, Laurel S., Harro, Janette M., Li, Xin, Lockatell, C.Virginia, Himpsl, Stephanie D., Hebel, J. Richard, ...
Proteus mirabilis, a common cause of urinary tract infections (UTI) in individuals with functional or structural abnormalities or with long-term catheterization, forms bladder and kidney stones as a...
Transcriptome of Uropathogenic Escherichia coli during Urinary Tract Infection
Snyder, Jennifer A., Haugen, Brian J., Buckles, Eric L., Lockatell, C. Virginia, Johnson, David E., Donnenberg, Michael S., ...
A uropathogenic Escherichia coli strain CFT073-specific DNA microarray that includes each open reading frame was used to analyze the transcriptome of CFT073 bacteria isolated directly from the urine...
Jansen, Angela M., Lockatell, Virginia, Johnson, David E., Mobley, Harry L. T.
Proteus mirabilis, an etiologic agent of complicated urinary tract infections, expresses mannose-resistant Proteus-like (MR/P) fimbriae whose expression is phase variable. Here we examine the role of...
Li, Xin, Erbe, Jarrod L., Lockatell, C. Virginia, Johnson, David E., Jobling, Michael G., Holmes, Randall K., ...
This is a follow-up to our previous study using an intranasal vaccine composed of MrpH, the tip adhesin of the MR/P fimbria, and cholera toxin to prevent urinary tract infection by Proteus mirabilis...
Johnson, James R., Jelacic, Srdjan, Schoening, Laura M., Clabots, Connie, Shaikh, Nurmohammad, Mobley, Harry L. T., ...
The role of the Escherichia coli iron-regulated gene homologue adhesin (Iha) in the pathogenesis of urinary tract infections (UTIs) is unknown. We performed a series of complementary analyses to...
Role of Motility in the Colonization of Uropathogenic Escherichia coli in the Urinary Tract
Lane, M. Chelsea, Lockatell, Virginia, Monterosso, Greta, Lamphier, Daniel, Weinert, Julia, Hebel, J. Richard, ...
Uropathogenic Escherichia coli (UPEC) causes most uncomplicated urinary tract infections (UTIs) in humans. Flagellum-mediated motility and chemotaxis have been suggested to contribute to virulence by...
Coordinate Expression of Fimbriae in Uropathogenic Escherichia coli
Snyder, Jennifer A., Haugen, Brian J., Lockatell, C. Virginia, Maroncle, Nathalie, Hagan, Erin C., Johnson, David E., ...
Uropathogenic Escherichia coli is the most common etiological agent of urinary tract infections. Bacteria can often express multiple adhesins during infection in order to favor attachment to specific...
Snyder, Jennifer A., Lloyd, Amanda L., Lockatell, C. Virginia, Johnson, David E., Mobley, Harry L. T.
Type 1 fimbrial phase-locked mutants of uropathogenic Escherichia coli cystitis isolate F11 were used to assess the role of the invertible element during urinary tract infection. Compared to the wild...
Testerman, Traci L., Conn, P. Brian, Mobley, Harry L. T., McGee, David J.
The growth of the gastric pathogen Helicobacter pylori in the absence of serum remains challenging, and nutritional requirements have only partially been defined, while almost nothing is known about...
Lane, M. Chelsea, Lloyd, Amanda L., Markyvech, Tiffany A., Hagan, Erin C., Mobley, Harry L. T.
The prevalence and function of four chemoreceptors, Tsr, Tar, Trg, and Tap, were determined for a collection of uropathogenic, fecal-commensal, and diarrheagenic Escherichia coli strains. tar and tsr...
Maroncle, Nathalie M., Sivick, Kelsey E., Brady, Rebecca, Stokes, Faye-Ellen, Mobley, Harry L. T.
The secreted autotransporter toxin (Sat), found predominantly in uropathogenic Escherichia coli, is a member of the SPATE (serine protease autotransporters of Enterobacteriaceae) family and, as such,...
Helicobacter pylori HP1512 Is a Nickel-Responsive NikR-Regulated Outer Membrane Protein▿
Davis, Gregg S., Flannery, Erika L., Mobley, Harry L. T.
Helicobacter pylori is dependent upon the production of the highly abundant and active metalloenzyme urease for colonization of the human stomach. Thus, H. pylori has an absolute requirement for the...
Defining Genomic Islands and Uropathogen-Specific Genes in Uropathogenic Escherichia coli▿
Lloyd, Amanda L., Rasko, David A., Mobley, Harry L. T.
Uropathogenic Escherichia coli (UPEC) strains are responsible for the majority of uncomplicated urinary tract infections, which can present clinically as cystitis or pyelonephritis. UPEC strain...
Alteri, Christopher J., Mobley, Harry L. T.
Outer membrane proteins (OMPs) of microbial pathogens are critical components that mediate direct interactions between microbes and their surrounding environment. Consequently, the study of OMPs is...
Lane, M. Chelsea, Simms, Amy N., Mobley, Harry L. T.
Type 1 fimbriae and flagella have been previously shown to contribute to the virulence of uropathogenic Escherichia coli (UPEC) within the urinary tract. In this study, the relationship between...
Uropathogenic Escherichia coli Outer Membrane Antigens Expressed during Urinary Tract Infection▿
Hagan, Erin C., Mobley, Harry L. T.
Uncomplicated urinary tract infection (UTI) caused by uropathogenic Escherichia coli (UPEC) represents a prevalent and potentially severe infectious disease. In this study, we describe the...
Lane, M. Chelsea, Alteri, Christopher J., Smith, Sara N., Mobley, Harry L. T.
Uropathogenic Escherichia coli (UPEC) cause most uncomplicated urinary tract infections (UTIs) in humans. Because UTIs are considered to occur in an ascending manner, flagellum-mediated motility has...
Simms, Amy N., Mobley, Harry L. T.
Two surface organelles of uropathogenic Escherichia coli (UPEC), flagella and type 1 fimbriae, are critical for colonization of the urinary tract but mediate opposite actions. Flagella propel...
Pearson, Melanie M., Sebaihia, Mohammed, Churcher, Carol, Quail, Michael A., Seshasayee, Aswin S., Luscombe, Nicholas M., ...
The gram-negative enteric bacterium Proteus mirabilis is a frequent cause of urinary tract infections in individuals with long-term indwelling catheters or with complicated urinary tracts (e.g., due...
Nielubowicz, Greta R., Smith, Sara N., Mobley, Harry L. T.
Proteus mirabilis, a gram-negative bacterium, is a frequent cause of complicated urinary tract infections in those with functional or anatomical abnormalities or those subject to long-term...
PapX, a P Fimbrial Operon-Encoded Inhibitor of Motility in Uropathogenic Escherichia coli▿
Simms, Amy N., Mobley, Harry L. T.
Motility and adherence are two integral aspects of bacterial pathogenesis. Adherence, often mediated by fimbriae, permits bacteria to attach to host cells and establish infection, whereas...
Alteri, Christopher J., Smith, Sara N., Mobley, Harry L. T.
Microbial pathogenesis studies traditionally encompass dissection of virulence properties such as the bacterium's ability to elaborate toxins, adhere to and invade host cells, cause tissue damage, or...
Alamuri, Praveen, Eaton, Kathryn A., Himpsl, Stephanie D., Smith, Sara N., Mobley, Harry L. T.
Complicated urinary tract infections (UTI) caused by Proteus mirabilis are associated with severe pathology in the bladder and kidney. To investigate the roles of two established cytotoxins, the HpmA...
Lane, M. Chelsea, Li, Xin, Pearson, Melanie M., Simms, Amy N., Mobley, Harry L. T.
MR/P fimbriae of uropathogenic Proteus mirabilis undergo invertible element-mediated phase variation whereby an individual bacterium switches between expressing fimbriae (phase ON) and not expressing...
Mucosal Immunization with Iron Receptor Antigens Protects against Urinary Tract Infection
Alteri, Christopher J., Hagan, Erin C., Sivick, Kelsey E., Smith, Sara N., Mobley, Harry L. T.
Uncomplicated infections of the urinary tract, caused by uropathogenic Escherichia coli, are among the most common diseases requiring medical intervention. A preventive vaccine to reduce the...