Yamashita, Hiroshi, Sugiura, Seiryo, Fujita, Hideo, Yasuda, So-ichiro, Nagai, Ryozo, Saeki, Yasutake, ...
Objective: To investigate the functional role of myosin light chain (MLC) isoforms in cardiac muscles, we examined the motor function of two different myosins the structure of which differed only in...
Suzuki, Suechika, Nagayoshi, Hayato, Ishino, Kohsuke, Hino, Naoki, Sugi, Haruo
The ultrastructural basis for the extremely rapid contraction–relaxation cycle (up to 300 s−1) in the swim‐bladder muscle (SBM) of a scorpionfish (Sebastiscus marmoratus), producing...
Oiwa, Kazuhiro, Kawakami, Toshimitsu, Sugi, Haruo
To obtain information about the mechanism of ATP-dependent actin-myosin sliding responsible for muscle contraction, we studied the “unitary” distance of sliding between a myosin-coated glass...
SUZUKI, Suechika, TSUCHIYA, Teizo, OSHIMI, Yoko, TAKEI, Takashi, SUGI, Haruo
To study the effect of stretch on the hexagonal myofilament lattice in frog skeletal muscle fibers, the fibers were fixed at rest and during the isometric tetanus with or without stretch, and their...
CHAEN, Shigeru, SHIMADA, Mitsuyo, SUGI, Haruo
Fluorescence spectra of ANM-labeled, glycerinated rabbit psoas muscle fibers were recorded in relaxed, contracted, and rigor states. SDS polyacrylamide gel electro-phoresis of the ANM-labeled muscle...
TANAKA, Hidehiro, TANAKA, Miyako, SUGI, Haruo
The effect of sarcomere length and stretching on the tension and the rate of ATP splitting was studied using small fiber bundles from glycerinated rabbit psoas muscle. The rate of ATP splitting was...
Sugi, Haruo, Iwamoto, Hiroyuki, Akimoto, Tsuyoshi, Ushitani, Hiroko
Although a contracting muscle regulates its energy output depending on the load imposed on it (“Fenn effect”), the mechanism underlying the load-dependent energy output remains obscure. To...
Dynamic electron microscopy of ATP-induced myosin head movement in living muscle thick filaments
Sugi, Haruo, Akimoto, Tsuyoshi, Sutoh, Kazuo, Chaen, Shigeru, Oishi, Noboru, Suzuki, Suechika
Although muscle contraction is known to result from movement of the myosin heads on the thick filaments while attached to the thin filaments, the myosin head movement coupled with ATP hydrolysis...
Sugi, Haruo, Iwamoto, Hiroyuki, Akimoto, Tsuyoshi, Ushitani, Hiroko
Although a contracting muscle regulates its energy output depending on the load imposed on it (“Fenn effect”), the mechanism underlying the load-dependent energy output remains obscure. To...
Dynamic electron microscopy of ATP-induced myosin head movement in living muscle thick filaments
Sugi, Haruo, Akimoto, Tsuyoshi, Sutoh, Kazuo, Chaen, Shigeru, Oishi, Noboru, Suzuki, Suechika
Although muscle contraction is known to result from movement of the myosin heads on the thick filaments while attached to the thin filaments, the myosin head movement coupled with ATP hydrolysis...
Isolated single frog muscle fibers were locally activated by applying negative current pulses to a pipette whose tip was in contact with the fiber surface. In contrast to the graded inward spread of...
Isolated single crayfish muscle fibers were locally activated by applying negative current pulses to a pipette whose tip was in contact with the fiber surface. The contraction initiated by a moderate...
Sugi, Haruo, Minoda, Hiroki, Inayoshi, Yuhri, Yumoto, Fumiaki, Miyakawa, Takuya, Miyauchi, Yumiko, ...
Despite >50 years of research work since the discovery of sliding filament mechanism in muscle contraction, structural details of the coupling of cyclic cross-bridge movement to ATP hydrolysis are...