Haruyuki Hirata

Detection of Antibodies to Neospora caninum in Cattle by Enzyme-Linked Immunosorbent Assay with Truncated NcSRS2 Expressed in Escherichia coli (2005)

Irungu Gaturaga, Bayin Chahan, Xuenan Xuan, Xiaohong Huang, Min Liao, Shinya Fukumoto, ...

The surface antigen 1–related sequence 2 of Neospora caninum (NcSRS2) is considered as an immunodominant antigen. In this study, the gene encoding truncated NcSRS2 (NcSRS2t) lacking an N-terminal...

Cellular Localization of Babesia bovis Merozoite Rhoptry-Associated Protein 1 and Its Erythrocyte-Binding Activity

Yokoyama, Naoaki, Suthisak, Boonchit, Hirata, Haruyuki, Matsuo, Tomohide, Inoue, Noboru, Sugimoto, Chihiro, ...

The cellular localization of Babesia bovis rhoptry-associated protein 1 (RAP-1) and its erythrocyte-binding affinity were examined with anti-RAP-1 antibodies. In an indirect immunofluorescent...

Cloning of a Truncated Babesia equi Gene Encoding an 82-Kilodalton Protein and Its Potential Use in an Enzyme-Linked Immunosorbent Assay

Hirata, Haruyuki, Ikadai, Hiromi, Yokoyama, Naoaki, Xuan, Xuenan, Fujisaki, Kozo, Suzuki, Naoyoshi, ...

To isolate Babesia equi genes encoding immunodominant proteins, a cDNA expression library prepared from B. equi mRNA was immunoscreened with B. equi-infected horse serum. Eighteen positive cDNA...

Roles of the Maltese Cross Form in the Development of Parasitemia and Protection against Babesia microti Infection in Mice

Yokoyama, Naoaki, Bork, Sabine, Nishisaka, Mitsuhiro, Hirata, Haruyuki, Matsuo, Tomohide, Inoue, Noboru, ...

Babesia microti, a hemoprotozoan parasite of rodents, is also important as a zoonotic agent of human babesiosis. The Maltese cross form, which consists of four masses in an erythrocyte, is...

Identification of a Specific Antigenic Region of the P82 Protein of Babesia equi and Its Potential Use in Serodiagnosis

Hirata, Haruyuki, Xuan, Xuenan, Yokoyama, Naoaki, Nishikawa, Yoshifumi, Fujisaki, Kozo, Suzuki, Naoyoshi, ...

The efficacy of the Be82 gene product fused with glutathione S-transferase (GST/Be82) in an enzyme-linked immunosorbent assay (ELISA) for the diagnosis of Babesia equi infection was reported...

Growth-Inhibitory Effects of Artesunate, Pyrimethamine, and Pamaquine against Babesia equi and Babesia caballi in In Vitro Cultures

Nagai, Akiko, Yokoyama, Naoaki, Matsuo, Tomohide, Bork, Sabine, Hirata, Haruyuki, Xuan, Xuenan, ...

Three antimalarial drugs, artesunate, pyrimethamine, and pamaquine, were evaluated for their growth-inhibitory effects against Babesia equi and Babesia caballi in in vitro culture. B. equi was more...

Molecular Cloning of a Babesia caballi Gene Encoding the 134-Kilodalton Protein and Evaluation of Its Diagnostic Potential in an Enzyme-Linked Immunosorbent Assay

Tamaki, Yoh, Hirata, Haruyuki, Takabatake, Noriyuki, Bork, Sabine, Yokoyama, Naoaki, Xuan, Xuenan, ...

A Babesia caballi gene encoding the 134-kDa (BC134) protein was immunoscreened with B. caballi-infected horse serum. An enzyme-linked immunosorbent assay (ELISA) using recombinant BC134 protein could...

Rapid Immunochromatographic Test Using Recombinant SAG2 for Detection of Antibodies against Toxoplasma gondii in Cats

Huang, Xiaohong, Xuan, Xuenan, Hirata, Haruyuki, Yokoyama, Naoaki, Xu, Longshan, Suzuki, Naoyoshi, ...

An immunochromatographic test using recombinant truncated surface antigen 2 for detection of antibodies against Toxoplasma gondii was developed. Evaluation of detection of the antibody in mice and...

Cloning of a Novel Babesia equi Gene Encoding a 158-Kilodalton Protein Useful for Serological Diagnosis

Hirata, Haruyuki, Yokoyama, Naoaki, Xuan, Xuenan, Fujisaki, Kozo, Suzuki, Naoyoshi, Igarashi, Ikuo

In this study, we characterized a Babesia equi Be158 gene obtained by immunoscreening a B. equi cDNA expression phage library with B. equi-infected horse serum. The Be158 gene consists of an open...

Cellular Localization of Babesia bovis Merozoite Rhoptry-Associated Protein 1 and Its Erythrocyte-Binding Activity

Yokoyama, Naoaki, Suthisak, Boonchit, Hirata, Haruyuki, Matsuo, Tomohide, Inoue, Noboru, Sugimoto, Chihiro, ...

The cellular localization of Babesia bovis rhoptry-associated protein 1 (RAP-1) and its erythrocyte-binding affinity were examined with anti-RAP-1 antibodies. In an indirect immunofluorescent...

Cloning of a Truncated Babesia equi Gene Encoding an 82-Kilodalton Protein and Its Potential Use in an Enzyme-Linked Immunosorbent Assay

Hirata, Haruyuki, Ikadai, Hiromi, Yokoyama, Naoaki, Xuan, Xuenan, Fujisaki, Kozo, Suzuki, Naoyoshi, ...

To isolate Babesia equi genes encoding immunodominant proteins, a cDNA expression library prepared from B. equi mRNA was immunoscreened with B. equi-infected horse serum. Eighteen positive cDNA...

Roles of the Maltese Cross Form in the Development of Parasitemia and Protection against Babesia microti Infection in Mice

Yokoyama, Naoaki, Bork, Sabine, Nishisaka, Mitsuhiro, Hirata, Haruyuki, Matsuo, Tomohide, Inoue, Noboru, ...

Babesia microti, a hemoprotozoan parasite of rodents, is also important as a zoonotic agent of human babesiosis. The Maltese cross form, which consists of four masses in an erythrocyte, is...

Identification of a Specific Antigenic Region of the P82 Protein of Babesia equi and Its Potential Use in Serodiagnosis

Hirata, Haruyuki, Xuan, Xuenan, Yokoyama, Naoaki, Nishikawa, Yoshifumi, Fujisaki, Kozo, Suzuki, Naoyoshi, ...

The efficacy of the Be82 gene product fused with glutathione S-transferase (GST/Be82) in an enzyme-linked immunosorbent assay (ELISA) for the diagnosis of Babesia equi infection was reported...

Growth-Inhibitory Effects of Artesunate, Pyrimethamine, and Pamaquine against Babesia equi and Babesia caballi in In Vitro Cultures

Nagai, Akiko, Yokoyama, Naoaki, Matsuo, Tomohide, Bork, Sabine, Hirata, Haruyuki, Xuan, Xuenan, ...

Three antimalarial drugs, artesunate, pyrimethamine, and pamaquine, were evaluated for their growth-inhibitory effects against Babesia equi and Babesia caballi in in vitro culture. B. equi was more...

Molecular Cloning of a Babesia caballi Gene Encoding the 134-Kilodalton Protein and Evaluation of Its Diagnostic Potential in an Enzyme-Linked Immunosorbent Assay

Tamaki, Yoh, Hirata, Haruyuki, Takabatake, Noriyuki, Bork, Sabine, Yokoyama, Naoaki, Xuan, Xuenan, ...

A Babesia caballi gene encoding the 134-kDa (BC134) protein was immunoscreened with B. caballi-infected horse serum. An enzyme-linked immunosorbent assay (ELISA) using recombinant BC134 protein could...

Rapid Immunochromatographic Test Using Recombinant SAG2 for Detection of Antibodies against Toxoplasma gondii in Cats

Huang, Xiaohong, Xuan, Xuenan, Hirata, Haruyuki, Yokoyama, Naoaki, Xu, Longshan, Suzuki, Naoyoshi, ...

An immunochromatographic test using recombinant truncated surface antigen 2 for detection of antibodies against Toxoplasma gondii was developed. Evaluation of detection of the antibody in mice and...

Cloning of a Novel Babesia equi Gene Encoding a 158-Kilodalton Protein Useful for Serological Diagnosis

Hirata, Haruyuki, Yokoyama, Naoaki, Xuan, Xuenan, Fujisaki, Kozo, Suzuki, Naoyoshi, Igarashi, Ikuo

In this study, we characterized a Babesia equi Be158 gene obtained by immunoscreening a B. equi cDNA expression phage library with B. equi-infected horse serum. The Be158 gene consists of an open...