Helmut Blöcker

Properties of a mutant lactose carrier of Escherichia coli with a Cys148 → Ser148 substitution (2009)

Neuhaus, Jean-Marc, Soppa, Jörg, Wright, J.Keith, Riede, Isolde, Blöcker, Helmut, Frank, Ronand, ...

The cysteine residue at position 148 in the lactose carrier protein of Escherichia coli has been replaced by serine using oligonucleotide-directed, site-specific mutagenesis of the lacY gene. The...

Properties of a mutant lactose carrier of Escherichia coli with a Cys148 → Ser148 substitution (2009)

Neuhaus, Jean-Marc, Soppa, Jörg, Wright, J.Keith, Riede, Isolde, Blöcker, Helmut, Frank, Ronand, ...

The cysteine residue at position 148 in the lactose carrier protein of Escherichia coli has been replaced by serine using oligonucleotide-directed, site-specific mutagenesis of the lacY gene. The...

The missing link: Bordetella petriiis endowed with both the metabolic versatility of environmental bacteria and virulence traits of pathogenic Bordetellae (2008)

Gross, Roy, Guzman, Carlos A, Sebaihia, Mohammed, Martins Dos Santos, Vítor AP, Pieper, Dietmar H, Koebnik, Ralf, ...

Abstract Background Bordetella petrii is the only environmental species hitherto found among the otherwise host-restricted and pathogenic members of the genus Bordetella . Phylogenetically, it...

BIOINFORMATICS Analysis of disturbed images (2008)

Gerhard Kauer, Helmut Blöcker

Motivation: Images in cellular and molecular biology (from microscopy, blots, biochips, etc.) are often disturbed, so that the detection and analysis of the respective relevant geometrical objects...

The full-ORF clone resource of the German cDNA Consortium (2007)

Bechtel, Stephanie, Rosenfelder, Heiko, Duda, Anny, Schmidt, Christian, Ernst, Ute, Wellenreuther, Ruth, ...

Abstract Background With the completion of the human genome sequence the functional analysis and characterization of the encoded proteins has become the next urging challenge in the post-genome era....

BMC Genomics Methodology article The full-ORF clone resource of the German cDNA Consortium (2007)

Stephanie Bechtel, Heiko Rosenfelder, Anny Duda, Christian Peter Schmidt, Ute Ernst, Ruth Wellenreuther, ...

© 2007 Bechtel et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License

FeatureScan: revealing property-dependent similarity of nucleotide sequences (2006)

Igor V. Deyneko, Björn Bredohl, Daniel Wesely, Yulia M. Kalybaeva, Er E. Kel, Helmut Blöcker, ...

FeatureScan is a software package aiming to reveal novel types of DNA sequence similarity by comparing physico-chemical properties. Thirtyeight different parameters of DNA double strands such as...

FeatureScan: revealing property-dependent similarity of nucleotide sequences (2006)

Igor V. Deyneko, Björn Bredohl, Daniel Wesely, Yulia M. Kalybaeva, Er E. Kel, Helmut Blöcker, ...

FeatureScan is a software package aiming to reveal novel types of DNA sequence similarity by comparing physico-chemical properties. Thirtyeight different parameters of DNA double strands such as...

FeatureScan: revealing property-dependent similarity of nucleotide sequences (2006)

Deyneko, Igor V., Bredohl, Björn, Wesely, Daniel, Kalybaeva, Yulia M., Kel, Alexander E., Blöcker, Helmut, ...

FeatureScan is a software package aiming to reveal novel types of DNA sequence similarity by comparing physico-chemical properties. Thirty-eight different parameters of DNA double strands such as...

Analysis of disturbed images (2004)

Kauer, Gerhard, Blöcker, Helmut

Motivation: Images in cellular and molecular biology (from microscopy, blots, biochips etc.) are often disturbed so that the detection and analysis of the respective relevant geometrical objects may...

Analysis of disturbed images (2004)

Kauer, Gerhard, Blöcker, Helmut

Motivation: Images in cellular and molecular biology (from microscopy, blots, biochips, etc.) are often disturbed, so that the detection and analysis of the respective relevant geometrical objects...

Analysis of disturbed images (2004)

Kauer, Gerhard, Blöcker, Helmut

Motivation: Images in cellular and molecular biology (from microscopy, blots, biochips etc.) are often disturbed so that the detection and analysis of the respective relevant geometrical objects may...

Applying signal theory to the analysis of biomolecules (2003)

Kauer, Gerhard, Blöcker, Helmut

Motivation: The accumulation of sequence-related and other biological data for basic research and application purposes invites disaster. It appears very likely that neither traditional thinking nor...

Grafting of discontinuous sites: a protein modeling strategy (1996)

Hornischer, Klaus, Blöcker, Helmut

A strategy for modeling continuous as well as discontinuous sites in protein structures has been developed. Central to this modeling strategy is the search algorithm of FITSITE, a program to search a...

The 'shortmer' approach to nucleic acid sequence analysis I: computer simulation of sequencing projects to find economical primer sets (1994)

Blöcker, Helmut, Lincoln, David N.

In principle it is most economical to sequence large DNA fragments consecutively (‘primer walking’), provided there is an immediate supply of sequencing primers. To solve the problem of primer...

Effect of a single 3'-methylene phosphonate linkage on the conformation of an A-DNA octamer double helix (1991)

Heinemann, Udo, Rudolph, Lif-Nicola, Alings, Claudia, Morr, Michael, Heikens, Wiebke, Frank, Ronald, ...

The three-dimensional structure of the self-complementary DNA octamer d(GCCCGpGGC) has been determined in the crystalline state using X-ray diffraction data to a nominal resolution of 2.12 measured...

Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA (1989)

Edwards, Ulrike, Rogall, Till, Blöcker, Helmut, Emde, Monica, Böttger, Erik C.

Using a set of synthetic oligonucleotides homologous to broadly conserved sequences in-vitro amplification via the polymerase chain reaction followed by direct sequencing results in almost complete...

Crystal structure analysis of an A-DNA fragment at 1.8 A resolution: d(GCCCGGGC) (1987)

Heinemann, Udo, Lauble, Hanspeter, Frank, Ronald, Blöcker, Helmut

Single crystals of the self-complementary octadeoxyribonucleotide d(GCCCGGGC) have been analysed by X-ray diffraction methods at a resolution of 1.8 Å. The tetragonal unit cell of space group P43212...

Role of thymidine residues in DNA recognition by the EcoRI and EcoRV restriction endonucleases (1986)

Fliess, Anja, Wolfes, Heiner, Rosenthal, Andre, Schwellnus, Konrad, Blöcker, Helmut, Frank, Ronald, ...

We have synthesized a series of oligonucleotides containing the EcoRI (GAATTC) or EcoRV (GATATC) recognition site within which or adjacent to which thymidine was substituted by uridine or derivatives...

Investigation of the tertiary folding of Escherichia coli 16s RNA by in situ intra-RNA crosslinking within 30S ribosomal subunits (1985)

Atmadja, Johannes, Brimacombe, Richard, Blöcker, Helmut, Frank, Ronald

Intra-RNA cross-links were introduced into E. coli 30S ribosomal subunits by mild ultraviolet irradiation. The subunits were partially digested with cobra venom nuclease, followed in some cases by a...

The 5'-terminal sequence of U1 RNA complementary to the consensus 5' splice site of hnRNA is single-stranded in intact U1 snRNP particles (1984)

Rinke, Jutta, Appel, Bernd, Blöcker, Helmut, Frank, Ronald, Lührmann, Reinhard

The 5′-terminal region of U1 snRNA is highly complementary to the consensus exon-intron regions of hnRNA and it has been suggested that U1 snRNP might play a role in the splicing of the pre-mRNA by...

A new general approach for the simultaneous chemical synthesis of large numbers of oligonucleotides: segmental solid supports (1983)

Frank, Ronald, Heikens, Wiebke, Heisterberg-Moutsis, Gudrun, Blöcker, Helmut

A new approach is described which will allow the simultaneous synthesis of large numbers of pre-defined oligonucleotide chains No machine aid is needed. The simultaneous syntheses can be performed by...

Ultrafast sequencing of oligodeoxyribonucleo tides by FAB-mass spectrometry (1982)

Grotjahn, Lutz, Frank, Ronald, Blöcker, Helmut

A fully instrumental method is described for the bidirectional sequencing of oligodeoxyribonucleotides. The methcd makes use of the negative ion fragmentation patterns of fast atom bombardment mass...

Toward a Catalog of Human Genes and Proteins: Sequencing and Analysis of 500 Novel Complete Protein Coding Human cDNAs

Wiemann, Stefan, Weil, Bernd, Wellenreuther, Ruth, Gassenhuber, Johannes, Glassl, Sabine, Ansorge, Wilhelm, ...

With the complete human genomic sequence being unraveled, the focus will shift to gene identification and to the functional analysis of gene products. The generation of a set of cDNAs, both sequences...

Gin-mediated site-specific recombination in bacteriophage Mu DNA: overproduction of the protein and inversion in vitro

Mertens, Gabriele, Hoffmann, Andrea, Blöcker, Helmut, Frank, Ronald, Kahmann, Regine

Inversion of the G segment in bacteriophage Mu DNA occurs by a site-specific recombination event and determines the host specificity of Mu phage particles produced. Inversion is mediated by a Mu...

FeatureScan: revealing property-dependent similarity of nucleotide sequences

Deyneko, Igor V., Bredohl, Björn, Wesely, Daniel, Kalybaeva, Yulia M., Kel, Alexander E., Blöcker, Helmut, ...

FeatureScan is a software package aiming to reveal novel types of DNA sequence similarity by comparing physico-chemical properties. Thirty-eight different parameters of DNA double strands such as...

Toward a Catalog of Human Genes and Proteins: Sequencing and Analysis of 500 Novel Complete Protein Coding Human cDNAs

Wiemann, Stefan, Weil, Bernd, Wellenreuther, Ruth, Gassenhuber, Johannes, Glassl, Sabine, Ansorge, Wilhelm, ...

With the complete human genomic sequence being unraveled, the focus will shift to gene identification and to the functional analysis of gene products. The generation of a set of cDNAs, both sequences...

Gin-mediated site-specific recombination in bacteriophage Mu DNA: overproduction of the protein and inversion in vitro

Mertens, Gabriele, Hoffmann, Andrea, Blöcker, Helmut, Frank, Ronald, Kahmann, Regine

Inversion of the G segment in bacteriophage Mu DNA occurs by a site-specific recombination event and determines the host specificity of Mu phage particles produced. Inversion is mediated by a Mu...

FeatureScan: revealing property-dependent similarity of nucleotide sequences

Deyneko, Igor V., Bredohl, Björn, Wesely, Daniel, Kalybaeva, Yulia M., Kel, Alexander E., Blöcker, Helmut, ...

FeatureScan is a software package aiming to reveal novel types of DNA sequence similarity by comparing physico-chemical properties. Thirty-eight different parameters of DNA double strands such as...