Hemalatha Balaram

Biochemical characterization of Plasmodium falciparum Sir2,a $NAD^+$-dependent deacetylase (2008)

Chakrabarty, Subhra Prakash, Saikumari, Yegnisettipalli Krishnaiah, Bopanna, Monnanda P, Balaram, Hemalatha

In Plasmodium falciparum, the causative agent of cerebral malaria, silent information regulator 2 (Sir2) has been implicated in pathogenesis through its role in var gene silencing. P. falciparum Sir2...

Structural biology of plasmodial proteins (2007)

Gayathri, P, Balaram, Hemalatha, Murthy, MRN

Malaria is a global disease infecting several million individuals annually. Malarial infection is particularly severe in the poorest parts of the world and is a major drain on their limited...

Structure of triosephosphate isomerase (TIM) from Methanocaldococcus jannaschii (2007)

Gayathri, P, Banerjee, Mousumi, Vijayalakshmi, A, Azeez, Shamina, Balaram, Hemalatha, Balaram, P, ...

The crystal structure of a recombinant triosephosphate isomerase (TIM) from the archaeabacterium Methanocaldococcus jannaschii has been determined at a resolution of 2.3 \AA using X-ray diffraction...

Structure of triosephosphate isomerase (TIM) from Methanocaldococcus jannaschii (2007)

Gayathri, P, Banerjee, Mousumi, Vijayalakshmi, A, Azeez, Shamina, Balaram, Hemalatha, Balaram, P, ...

The crystal structure of a recombinant triosephosphate isomerase (TIM) from the archaeabacterium Methanocaldococcus jannaschii has been determined at a resolution of 2.3 \AA using X-ray diffraction...

Structural biology of plasmodial proteins (2007)

Gayathri, P, Balaram, Hemalatha, Murthy, MRN

Malaria is a global disease infecting several million individuals annually. Malarial infection is particularly severe in the poorest parts of the world and is a major drain on their limited...

Structure of Plasmodium falciparum Triose-phosphate Isomerase-2-Phosphoglycerate Complex at 1.1-Å Resolution (2003)

Parthasarathy, Sampathkumar, Eaazhisai, Kandiah, Balaram, Hemalatha, Balaram, Padmanabhan, Murthy, Mathur RN

Triose-phosphate isomerase, a key enzyme of the glycolytic pathway, catalyzes the isomerization of dihydroxy acetone phosphate and glyceraldehyde 3-phosphate. In this communication we report the...

Stage-specific profiling of Plasmodium falciparum proteases using an internally quenched multispecificity protease substrate (2003)

Pattanaik, Priyaranjan, Jain, Bimba, Ravindra, Gudihal, Gopi, Hosahudya N, Pal, Prajna P, Balaram, Hemalatha, ...

Novel internally quenched fluorescence peptide substrates containing sequence specific sites for cleavage by multiple proteases were designed and synthesized. The 28 and 29 residue peptides contain...

Unusual fluorescence of W168 in Plasmodium falciparum triosephosphate isomerase, probed by single-tryptophan mutants (2003)

Pattanaik, Priyaranjan, Ravindra, Gudihal, Sengupta, Chandana, Maithal, Kapil, Balaram, Padmanabhan, Balaram, Hemalatha

Plasmodium falciparum triosephosphate isomerase (PfTIM) contains two tryptophan residues, W11 and W168. One is positioned in the interior of the protein, and the other is located on the active-site...

Proteolytic stability of beta-peptide bonds probed using quenched fluorescent substrates incorporating a hemoglobin cleavage site (2003)

Gopi, Hosahudya N, Ravindra, Gudihal, Pal, Prajna P, Pattanaik, Priyaranjan, Balaram, Hemalatha, Balaram, Padmanabhan

A set of designed internally quenched fluorescence peptide substrates has been used to probe the e¡ects of insertion of beta-peptide bonds into peptide sequences. The test sequence chosen...

Structure of Plasmodium falciparum Triose-phosphate Isomerase-2-Phosphoglycerate Complex at 1.1-Å Resolution (2003)

Parthasarathy, Sampathkumar, Eaazhisai, Kandiah, Balaram, Hemalatha, Balaram, Padmanabhan, Murthy, Mathur RN

Triose-phosphate isomerase, a key enzyme of the glycolytic pathway, catalyzes the isomerization of dihydroxy acetone phosphate and glyceraldehyde 3-phosphate. In this communication we report the...

Stage-specific profiling of Plasmodium falciparum proteases using an internally quenched multispecificity protease substrate (2003)

Pattanaik, Priyaranjan, Jain, Bimba, Ravindra, Gudihal, Gopi, Hosahudya N, Pal, Prajna P, Balaram, Hemalatha, ...

Novel internally quenched fluorescence peptide substrates containing sequence specific sites for cleavage by multiple proteases were designed and synthesized. The 28 and 29 residue peptides contain...

Unusual fluorescence of W168 in Plasmodium falciparum triosephosphate isomerase, probed by single-tryptophan mutants (2003)

Pattanaik, Priyaranjan, Ravindra, Gudihal, Sengupta, Chandana, Maithal, Kapil, Balaram, Padmanabhan, Balaram, Hemalatha

Plasmodium falciparum triosephosphate isomerase (PfTIM) contains two tryptophan residues, W11 and W168. One is positioned in the interior of the protein, and the other is located on the active-site...

Proteolytic stability of beta-peptide bonds probed using quenched fluorescent substrates incorporating a hemoglobin cleavage site (2003)

Gopi, Hosahudya N, Ravindra, Gudihal, Pal, Prajna P, Pattanaik, Priyaranjan, Balaram, Hemalatha, Balaram, Padmanabhan

A set of designed internally quenched fluorescence peptide substrates has been used to probe the e¡ects of insertion of beta-peptide bonds into peptide sequences. The test sequence chosen...

Structures of Plasmodium falciparum triosephosphate isomerase complexed to substrate analogues: observation of the catalytic loop in the open conformation in the ligand-bound state (2002)

Parthasarathy, S, Balaram, Hemalatha, Balaram, P, Murthy, MRN

The glycolytic enzymes of Plasmodium falciparum (Pf) are attractive drug targets as the parasites lack a functional tricarboxylic cycle and hence depend heavily on glycolysis for their energy...

Subunit interface mutation disrupting an aromatic cluster in Plasmodium falciparum triosephosphate isomerase: effect on dimer stability (2002)

Maithal, Kapil, Ravindra, Gudihal, Nagaraj, G, Singh, Kumar S, Balaram, Hemalatha, Balaram, P

A mutation at the dimer interface of Plasmodium falciparum triosephosphate isomerase (PfTIM) was created by mutating a tyrosine residue at position 74, at the subunit interface, to glycine. Tyr74 is...

Inhibition of Plasmodium falciparum Triose-phosphate Isomerase by Chemical Modification of an Interface Cysteine: Electrospray Ionization Mass Spectrometric Analysis of Differential Cysteine Reactivities (2002)

Maithal, Kapil, Ravindra, Gudihal, Balaram, Hemalatha, Balaram, Padmanabhan

Plasmodium falciparum triose-phosphate isomerase, a homodimeric enzyme, contains four cysteine residues at positions 13, 126, 196, and 217 per subunit. Among these, Cys-13 is present at the dimer...

Structures of Plasmodium falciparum triosephosphate isomerase complexed to substrate analogues: observation of the catalytic loop in the open conformation in the ligand-bound state (2002)

Parthasarathy, S, Balaram, Hemalatha, Balaram, P, Murthy, MRN

The glycolytic enzymes of Plasmodium falciparum (Pf) are attractive drug targets as the parasites lack a functional tricarboxylic cycle and hence depend heavily on glycolysis for their energy...

Subunit interface mutation disrupting an aromatic cluster in Plasmodium falciparum triosephosphate isomerase: effect on dimer stability (2002)

Maithal, Kapil, Ravindra, Gudihal, Nagaraj, G, Singh, Kumar S, Balaram, Hemalatha, Balaram, P

A mutation at the dimer interface of Plasmodium falciparum triosephosphate isomerase (PfTIM) was created by mutating a tyrosine residue at position 74, at the subunit interface, to glycine. Tyr74 is...

Inhibition of Plasmodium falciparum Triose-phosphate Isomerase by Chemical Modification of an Interface Cysteine: Electrospray Ionization Mass Spectrometric Analysis of Differential Cysteine Reactivities (2002)

Maithal, Kapil, Ravindra, Gudihal, Balaram, Hemalatha, Balaram, Padmanabhan

Plasmodium falciparum triose-phosphate isomerase, a homodimeric enzyme, contains four cysteine residues at positions 13, 126, 196, and 217 per subunit. Among these, Cys-13 is present at the dimer...

Synthetic peptides as inactivators of multimeric enzymes: inhibition of Plasmodium falciparum triosephosphate isomerase by interface peptides (2001)

Singh, Kumar S, Maithal, Kapil, Balaram, Hemalatha, Balaram, P

Synthetic peptides corresponding to two distinct segments of the subunit interface of the homodimeric enzyme triosephosphate isomerase (residues 9–18, ANWKCNGTLE, peptide I; residues 68–79,...

Antimalarial Activities of Peptide Antibiotics Isolated from Fungi (2001)

Nagaraj, G, Uma, MV, Shivayogi, MS, Balaram, Hemalatha

Malaria caused by Plasmodium falciparum is a major public health problem in the developing countries of the world. Clinical treatment of malaria has become complicated due to the occurrence of...

Synthetic peptides as inactivators of multimeric enzymes: inhibition of Plasmodium falciparum triosephosphate isomerase by interface peptides (2001)

Singh, Kumar S, Maithal, Kapil, Balaram, Hemalatha, Balaram, P

Synthetic peptides corresponding to two distinct segments of the subunit interface of the homodimeric enzyme triosephosphate isomerase (residues 9–18, ANWKCNGTLE, peptide I; residues 68–79,...

Antimalarial Activities of Peptide Antibiotics Isolated from Fungi (2001)

Nagaraj, G, Uma, MV, Shivayogi, MS, Balaram, Hemalatha

Malaria caused by Plasmodium falciparum is a major public health problem in the developing countries of the world. Clinical treatment of malaria has become complicated due to the occurrence of...

Unusual stability of a multiply nicked form of Plasmodium falciparum triosephosphate isomerase (1999)

Ray, Soumya S, Balaram, Hemalatha, Balaram, P

The limited proteolytic cleavage of proteins can result in distinct polypeptides that remain noncovalently associated so that the structural and biochemical properties of the ‘nicked’ protein are...

Unusual stability of a multiply nicked form of Plasmodium falciparum triosephosphate isomerase (1999)

Ray, Soumya S, Balaram, Hemalatha, Balaram, P

The limited proteolytic cleavage of proteins can result in distinct polypeptides that remain noncovalently associated so that the structural and biochemical properties of the ‘nicked’ protein are...

Triosephosphate isomerase from Plasmodium falciparum: the crystal structure provides insights into antimalarial drug design (1997)

Velanker, Sameer S, Ray, Soumya S, Gokhale, Rajesh S, Suma, S, Balaram, Hemalatha, Balaram, P, ...

Background: Malaria caused by the parasite Plasmodium falciparum is a major public health concern. The parasite lacks a functional tricarboxylic acid cycle, making glycolysis its sole energy source....

Triosephosphate isomerase from Plasmodium falciparum: the crystal structure provides insights into antimalarial drug design (1997)

Velanker, Sameer S, Ray, Soumya S, Gokhale, Rajesh S, Suma, S, Balaram, Hemalatha, Balaram, P, ...

Background: Malaria caused by the parasite Plasmodium falciparum is a major public health concern. The parasite lacks a functional tricarboxylic acid cycle, making glycolysis its sole energy source....

Peptide Design: Influence of a Guest Aib-Pro Segment on the Stereochemistry of an Oligo-Val Sequence-Solution Conformations and Crystal Structure of Boc-(Val)2-Aib-Pro-(Val)3-OMe (1990)

Karle, Isabella L, Flippen-Anderson, Judith L, Uma, K, Balaram, Hemalatha, Balaram, P

The peptide Boc-Val-Val-Aib-Pro-Val-Val-Val-OMe has been synthesized to investigate the effect of introduction of a strong \beta-turn promoting guest segment into an oligopeptide with a tendency to...

Peptide Design: Influence of a Guest Aib-Pro Segment on the Stereochemistry of an Oligo-Val Sequence-Solution Conformations and Crystal Structure of Boc-(Val)2-Aib-Pro-(Val)3-OMe (1990)

Karle, Isabella L, Flippen-Anderson, Judith L, Uma, K, Balaram, Hemalatha, Balaram, P

The peptide Boc-Val-Val-Aib-Pro-Val-Val-Val-OMe has been synthesized to investigate the effect of introduction of a strong \beta-turn promoting guest segment into an oligopeptide with a tendency to...

Comparison of the Effect of Five Guest Residues on the \beta-Sheet Conformation of Host ${(L-Val)}_n$ Oligopeptides (1989)

Moretto, V, Crisma, M, Bonora, GM, Toniolo, C, Balaram, Hemalatha, Balaram, P

The synthesis, characterization, and IR absorption, 'H NMR, and CD properties of ${(L-Val)}_n$, host tetra-, penta-, and hexapeptides containing an L-Ile, L-Ala, D-Val, L-Pro, or Aib guest residue...

\alpha -Helix and Mixed $3_{10}$/ \alpha -helix in Cocrystallized Conformers of Boc-Aib-Val-Aib-Aib-Val-Val-Val-Aib-Val-Aib-Ome (1989)

Karle, Isabella L, Flippen-Anderson, Judith L, Uma, Kuchibhotla, Balaram, Hemalatha, Balaram, Padmanabhan

Two molecules of Boc-Aib-Val-Aib-Aib-Val-Val-Val-Aib-Val-Aib-OMe (where Boc is t-butoxycarbonyl and Aib is \alpha-aminoisobutyryl) cocrystallize in a triclinic cell with different helical...

\alpha -Helix and Mixed $3_{10}$/ \alpha -helix in Cocrystallized Conformers of Boc-Aib-Val-Aib-Aib-Val-Val-Val-Aib-Val-Aib-Ome (1989)

Karle, Isabella L, Flippen-Anderson, Judith L, Uma, Kuchibhotla, Balaram, Hemalatha, Balaram, Padmanabhan

Two molecules of Boc-Aib-Val-Aib-Aib-Val-Val-Val-Aib-Val-Aib-OMe (where Boc is t-butoxycarbonyl and Aib is \alpha-aminoisobutyryl) cocrystallize in a triclinic cell with different helical...

Comparison of the Effect of Five Guest Residues on the \beta-Sheet Conformation of Host ${(L-Val)}_n$ Oligopeptides (1989)

Moretto, V, Crisma, M, Bonora, GM, Toniolo, C, Balaram, Hemalatha, Balaram, P

The synthesis, characterization, and IR absorption, 'H NMR, and CD properties of ${(L-Val)}_n$, host tetra-, penta-, and hexapeptides containing an L-Ile, L-Ala, D-Val, L-Pro, or Aib guest residue...

Stereochemistry of \alpha-Aminoisobutyric Acid Peptides in Solution: Conformations of Decapeptides with a Central Triplet of Contiguous L-Amino Acids (1986)

Balaram, Hemalatha, Sukumar, M, Balaram, P

The decapeptides $Boc-Aib-L-Val-Aib-Aib-{(L-Val)}_3-Aib-L-Val-Aib-OMe$ and $Boc-Aib-L-Leu- Aib-Aib-{(L-Leu)}_3-Aib-L-Leu-Aib-OMe$ have been studied in $CD{Cl}_3$ and ${({CD}_3)}_2SO$ solutions by...

Stereochemistry of \alpha-Aminoisobutyric Acid Peptides in Solution: Conformations of Decapeptides with a Central Triplet of Contiguous L-Amino Acids (1986)

Balaram, Hemalatha, Sukumar, M, Balaram, P

The decapeptides $Boc-Aib-L-Val-Aib-Aib-{(L-Val)}_3-Aib-L-Val-Aib-OMe$ and $Boc-Aib-L-Leu- Aib-Aib-{(L-Leu)}_3-Aib-L-Leu-Aib-OMe$ have been studied in $CD{Cl}_3$ and ${({CD}_3)}_2SO$ solutions by...

Multiple Conformational States of a Pro-Pro Peptide. Solid-State and Solution Conformations of Boc-Aib-Pro-Pro-NHMe (1983)

Balaram, Hemalatha, Prasad, Venkataram BV, Balaram, P

The solid-state and solution conformations of the model peptide Boc-Aib-Pro-Pro-NHMe have been studied by X-ray diffraction and NMR. The peptide adopts a poly(pro1ine 11) conformation in the solid...

Multiple Conformational States of a Pro-Pro Peptide. Solid-State and Solution Conformations of Boc-Aib-Pro-Pro-NHMe (1983)

Balaram, Hemalatha, Prasad, Venkataram BV, Balaram, P

The solid-state and solution conformations of the model peptide Boc-Aib-Pro-Pro-NHMe have been studied by X-ray diffraction and NMR. The peptide adopts a poly(pro1ine 11) conformation in the solid...

Nuclear Overhauser Effects and circular Dichroism as Probes of \beta.-turn Conformations in Acyclic and Cyclic Peptides with Pro-X Sequences (1983)

Rao, Narasinga BN, Kumar, Anil, Balaram, Hemalatha, Ravi, A, Balaram, P

Nuclear Overhauser effects (NOE) and (CD) techniques have been used to probe \beta-turn conformations in acyclic and cyclic peptides containing Pro-X sequences. The model peptides studied are of the...

Nuclear Overhauser Effects and circular Dichroism as Probes of \beta.-turn Conformations in Acyclic and Cyclic Peptides with Pro-X Sequences (1983)

Rao, Narasinga BN, Kumar, Anil, Balaram, Hemalatha, Ravi, A, Balaram, P

Nuclear Overhauser effects (NOE) and (CD) techniques have been used to probe \beta-turn conformations in acyclic and cyclic peptides containing Pro-X sequences. The model peptides studied are of the...

Type II \beta-Turn Conformation of Pivaloyl-L-Prolyl-\alpha-Aminoisobutyryl-N-Methylamide: Theoretical, Spectroscopic, and X-Ray Studies (1982)

Prasad, Venkataram BV, Balaram, Hemalatha, Balaram, P

Pivaloyl-L-Pro-Aib-N-methylamide has been shown to possess one intramolecular hydrogen bond in ${({CD}_3)}_2SO$ solution, by 'H-nmr methods, suggesting the existence of \beta-turns, with Pro-Aib as...

Type II \beta-Turn Conformation of Pivaloyl-L-Prolyl-\alpha-Aminoisobutyryl-N-Methylamide: Theoretical, Spectroscopic, and X-Ray Studies (1982)

Prasad, Venkataram BV, Balaram, Hemalatha, Balaram, P

Pivaloyl-L-Pro-Aib-N-methylamide has been shown to possess one intramolecular hydrogen bond in ${({CD}_3)}_2SO$ solution, by 'H-nmr methods, suggesting the existence of \beta-turns, with Pro-Aib as...

Antimalarial Activities of Peptide Antibiotics Isolated from Fungi

Nagaraj, G., Uma, M. V., Shivayogi, M. S., Balaram, Hemalatha

Malaria caused by Plasmodium falciparum is a major public health problem in the developing countries of the world. Clinical treatment of malaria has become complicated due to the occurrence of...

Antimalarial Activities of Peptide Antibiotics Isolated from Fungi

Nagaraj, G., Uma, M. V., Shivayogi, M. S., Balaram, Hemalatha

Malaria caused by Plasmodium falciparum is a major public health problem in the developing countries of the world. Clinical treatment of malaria has become complicated due to the occurrence of...