MODOMICS: a database of RNA modification pathways. 2008 update (2009)
Czerwoniec, Anna, Dunin-Horkawicz, Stanislaw, Purta, Elzbieta, Kaminska, Katarzyna H., Kasprzak, Joanna M., Bujnicki, Janusz M., ...
MODOMICS, a database devoted to the systems biology of RNA modification, has been subjected to substantial improvements. It provides comprehensive information on the chemical structure of modified...
Grosjean, Henri, Gaspin, Christine, Marck, Christian, Decatur, Wayne A
Abstract Background Naturally occurring RNAs contain numerous enzymatically altered nucleosides. Differences in RNA populations (RNomics) and pattern of RNA modifications (Modomics) depends on the...
RNomics and Modomics in the halophilic archaea Haloferax volcanii: (2008)
Bmc Genomics, Henri Grosjean, Christine Gaspin, Christian Marck, Wayne A Decatur
identification of RNA modification genes
Walbott, Hélène, Leulliot, Nicolas, Grosjean, Henri, Golinelli-Pimpaneau, Béatrice
The 5-methyluridine is invariably found at position 54 in the TΨC loop of tRNAs of most organisms. In Pyrococcus abyssi, its formation is catalyzed by the S-adenosyl-l-methionine-dependent tRNA...
Grosjean, Henri, Marck, Christian
Over a thousand of cytoplasmic, non organellar tRNA genes were extracted from the whole-genomes of more than 100 organisms spanning the Bacteria, Eukarya and Archaea (tRNomics). Also, whenever...
THUMP from archaeal tRNA:m22G10 methyltransferase, a genuine autonomously folding domain (2006)
Gabant, Guillaume, Auxilien, Sylvie, Tuszynska, Irina, Locard, Marie, Gajda, Michal J., Chaussinand, Guylaine, ...
The tRNA:m22G10 methyltransferase of Pyrococus abyssi (PAB1283, a member of COG1041) catalyzes the N2,N2-dimethylation of guanosine at position 10 in tRNA. Boundaries of its THUMP (THioUridine...
Formation of the conserved pseudouridine at position 55 in archaeal tRNA (2006)
Roovers, Martine, Hale, Caryn, Tricot, Catherine, Terns, Michael P., Terns, Rebecca M., Grosjean, Henri, ...
Pseudouridine (Ψ) located at position 55 in tRNA is a nearly universally conserved RNA modification found in all three domains of life. This modification is catalyzed by TruB in bacteria and by Pus4...
Formation of the conserved pseudouridine at position 55 in archaeal tRNA (2006)
Roovers, Martine, Hale, Caryn, Tricot, Catherine, Terns, Michael P., Terns, Rebecca M., Grosjean, Henri, ...
Pseudouridine (Ψ) located at position 55 in tRNA is a nearly universally conserved RNA modification found in all three domains of life. This modification is catalyzed by TruB in bacteria and by Pus4...
Marck, Christian, Kachouri-Lafond, Rym, Lafontaine, Ingrid, Westhof, Eric, Dujon, Bernard, Grosjean, Henri
We present the first comprehensive analysis of RNA polymerase III (Pol III) transcribed genes in ten yeast genomes. This set includes all tRNA genes (tDNA) and genes coding for SNR6 (U6), SNR52, SCR1...
MODOMICS: a database of RNA modification pathways (2006)
Dunin-Horkawicz, Stanislaw, Czerwoniec, Anna, Gajda, Michal J., Feder, Marcin, Grosjean, Henri, Bujnicki, Janusz M.
MODOMICS is the first comprehensive database resource for systems biology of RNA modification. It integrates information about the chemical structure of modified nucleosides, their localization in...
Formation of the conserved pseudouridine at position 55 in archaeal tRNA (2006)
Roovers, Martine, Hale, Caryn, Tricot, Catherine, Terns, Michael P., Terns, Rebecca M., Grosjean, Henri, ...
Pseudouridine (Ψ) located at position 55 in tRNA is a nearly universally conserved RNA modification found in all three domains of life. This modification is catalyzed by TruB in bacteria and by Pus4...
Urbonavicius, Jaunius, Skouloubris, Stéphane, Myllykallio, Hannu, Grosjean, Henri
Formation of 5-methyluridine (ribothymidine) at position 54 of the T-psi loop of tRNA is catalyzed by site-specific tRNA methyltransferases (tRNA:m5U-54 MTase). In all Eukarya and many Gram-negative...
A primordial RNA modification enzyme: the case of tRNA (m1A) methyltransferase (2004)
Roovers, Martine, Wouters, Johan, Bujnicki, Janusz M., Tricot, Catherine, Stalon, Victor, Grosjean, Henri, ...
The modified nucleoside 1‐methyladenosine (m1A) is found in the T‐loop of many tRNAs from organisms belonging to the three domains of life (Eukaryota, Bacteria, Archaea). In the T‐loop...
Droogmans, Louis, Roovers, Martine, Bujnicki, Janusz M., Tricot, Catherine, Hartsch, Thomas, Stalon, Victor, ...
N1‐methyladenosine (m1A) is found at position 58 in the T‐loop of many tRNAs. In yeast, the formation of this modified nucleoside is catalyzed by the essential tRNA (m1A58)...
Hellmuth, Klaus, Grosjean, Henri, Motorin, Yuri, Deinert, Karina, Hurt, Ed, Simos, George
Saccharomyces cerevisiae cells that carry deletions in both the LOS1 (a tRNA export receptor) and the PUS1 (a tRNA:pseudouridine synthase) genes exhibit a thermosensitive growth defect. A...
Modified nucleotides in Bacillus subtilis tRNATrp hyperexpressed in Escherichia coli (1993)
Xue, Hong, Glasser, Anne-Lise, Desgres, Jean, Grosjean, Henri
In the present study, modified nucleotides in the B.subtilis tRNATrp cloned and hyperexpressed In E.coll have been identified by TLC and HPLC analyses. The modification patterns of the two...
Pleiotrophic effects of point mutations in yeast tRNAAsp on the base modification pattern (1993)
Edqvist, Johan, Sträby, Kerstin B., Grosjean, Henri
The base-modification pattern has been studied in several synthetic variants of yeast tRNAAsp injected into Xenopus laevis oocytes. Certain point mutations in the D-stem and the varlabie loop of the...
Identity elements for N2-dimethylation of guanosine-26 in yeast tRNAs (1992)
Edqvist, Johan, Grosjean, Henri, Stråby, Kerstin B.
N2, N2-=dimethylguanosine (m2/2G) is a characteristic nucleoside that is found in the bend between the dihydro-uridine (D) stem and the anticodon (AC) stem in over 80% of the eukaryotic tRNA species...
Haumont, Etienne, Fournier, Michel, Henau, Suzy De, Grosjean, Henri
We have investigated the specificity of the tRNA modifying enzyme that transforms the adenosine at position 34 (wobble position) into inosine in the anticodon of several tRNAs. For this purpose, we...
Fournier, Michel, Haumont, Etienne, Henau, Suzy De, Gangloff, Jean, Grosjean, Henri
An enzymatic procedure for the replacement of the ICG anticodon of yeast tRNAλJg by NCG tnnucleotide (N=A, C, G or U) is described. Partial digestion with Si-nuclease and Ti-RNAase provides...
Nonsense suppression in eukaryotes: the use of the Xenopus oocyte as an in vivo assay system (1980)
Bienz, Mariann, Kubli, Eric, Jürg, Kohli, De Henau, Suzanne, Grosjean, Henri
Amber, ochre, and opal nonsense suppressor tRNAs isolated from yeast were injected into Xenopus laevis oocytes together with purified mRNAs (globin mRNA from rabbit, tobacco mosaic virus-RNA). Yeast...
Massenet, Séverine, Motorin, Yuri, Lafontaine, Denis L. J., Hurt, Eduard C., Grosjean, Henri, Branlant, Christiane
Pseudouridine (Ψ) residues were localized in the Saccharomyces cerevisiae spliceosomal U small nuclear RNAs (UsnRNAs) by using the chemical mapping method. In contrast to vertebrate UsnRNAs, S....
Cloning and characterization of the Schizosaccharomyces pombe tRNA:pseudouridine synthase Pus1p
Hellmuth, Klaus, Grosjean, Henri, Motorin, Yuri, Deinert, Karina, Hurt, Ed, Simos, George
Saccharomyces cerevisiae cells that carry deletions in both the LOS1 (a tRNA export receptor) and the PUS1 (a tRNA:pseudouridine synthase) genes exhibit a thermosensitive growth defect. A...
Trm7p catalyses the formation of two 2′-O-methylriboses in yeast tRNA anticodon loop
Pintard, Lionel, Lecointe, François, Bujnicki, Janusz M., Bonnerot, Claire, Grosjean, Henri, Lapeyre, Bruno
The genome of Saccharomyces cerevisiae encodes three close homologues of the Escherichia coli 2′-O-rRNA methyltransferase FtsJ/RrmJ, designated Trm7p, Spb1p and Mrm2p. We present evidence that...
Droogmans, Louis, Roovers, Martine, Bujnicki, Janusz M., Tricot, Catherine, Hartsch, Thomas, Stalon, Victor, ...
N1-methyladenosine (m1A) is found at position 58 in the T-loop of many tRNAs. In yeast, the formation of this modified nucleoside is catalyzed by the essential tRNA (m1A58) methyltransferase, a...
A primordial RNA modification enzyme: the case of tRNA (m1A) methyltransferase
Roovers, Martine, Wouters, Johan, Bujnicki, Janusz M., Tricot, Catherine, Stalon, Victor, Grosjean, Henri, ...
The modified nucleoside 1-methyladenosine (m1A) is found in the T-loop of many tRNAs from organisms belonging to the three domains of life (Eukaryota, Bacteria, Archaea). In the T-loop of eukaryotic...
Purushothaman, Suresh K., Bujnicki, Janusz M., Grosjean, Henri, Lapeyre, Bruno
N2-Monomethylguanosine-10 (m2G10) and N2,N2-dimethylguanosine-26 (m22G26) are the only two guanosine modifications that have been detected in tRNA from nearly all archaea and eukaryotes but not in...
Urbonavičius, Jaunius, Skouloubris, Stéphane, Myllykallio, Hannu, Grosjean, Henri
Formation of 5-methyluridine (ribothymidine) at position 54 of the T-psi loop of tRNA is catalyzed by site-specific tRNA methyltransferases (tRNA:m5U-54 MTase). In all Eukarya and many Gram-negative...
MODOMICS: a database of RNA modification pathways
Dunin-Horkawicz, Stanislaw, Czerwoniec, Anna, Gajda, Michal J., Feder, Marcin, Grosjean, Henri, Bujnicki, Janusz M.
MODOMICS is the first comprehensive database resource for systems biology of RNA modification. It integrates information about the chemical structure of modified nucleosides, their localization in...
Marck, Christian, Grosjean, Henri
From 50 genomes of the three domains of life (7 eukarya, 13 archaea, and 30 bacteria), we extracted, analyzed, and compared over 4,000 sequences corresponding to cytoplasmic, nonorganellar tRNAs. For...
MARCK, CHRISTIAN, GROSJEAN, HENRI
Most introns of archaeal tRNA genes (tDNAs) are located in the anticodon loop, between nucleotides 37 and 38, the unique location of their eukaryotic counterparts. However, in several Archaea, mostly...
Marck, Christian, Kachouri-Lafond, Rym, Lafontaine, Ingrid, Westhof, Eric, Dujon, Bernard, Grosjean, Henri
We present the first comprehensive analysis of RNA polymerase III (Pol III) transcribed genes in ten yeast genomes. This set includes all tRNA genes (tDNA) and genes coding for SNR6 (U6), SNR52, SCR1...
THUMP from archaeal tRNA:m22G10 methyltransferase, a genuine autonomously folding domain
Gabant, Guillaume, Auxilien, Sylvie, Tuszynska, Irina, Locard, Marie, Gajda, Michal J., Chaussinand, Guylaine, ...
The tRNA:m22G10 methyltransferase of Pyrococus abyssi (PAB1283, a member of COG1041) catalyzes the N2,N2-dimethylation of guanosine at position 10 in tRNA. Boundaries of its THUMP (THioUridine...
Formation of the conserved pseudouridine at position 55 in archaeal tRNA
Roovers, Martine, Hale, Caryn, Tricot, Catherine, Terns, Michael P., Terns, Rebecca M., Grosjean, Henri, ...
Pseudouridine (Ψ) located at position 55 in tRNA is a nearly universally conserved RNA modification found in all three domains of life. This modification is catalyzed by TruB in bacteria and by Pus4...
Massenet, Séverine, Motorin, Yuri, Lafontaine, Denis L. J., Hurt, Eduard C., Grosjean, Henri, Branlant, Christiane
Pseudouridine (Ψ) residues were localized in the Saccharomyces cerevisiae spliceosomal U small nuclear RNAs (UsnRNAs) by using the chemical mapping method. In contrast to vertebrate UsnRNAs, S....
Cloning and characterization of the Schizosaccharomyces pombe tRNA:pseudouridine synthase Pus1p
Hellmuth, Klaus, Grosjean, Henri, Motorin, Yuri, Deinert, Karina, Hurt, Ed, Simos, George
Saccharomyces cerevisiae cells that carry deletions in both the LOS1 (a tRNA export receptor) and the PUS1 (a tRNA:pseudouridine synthase) genes exhibit a thermosensitive growth defect. A...
Trm7p catalyses the formation of two 2′-O-methylriboses in yeast tRNA anticodon loop
Pintard, Lionel, Lecointe, François, Bujnicki, Janusz M., Bonnerot, Claire, Grosjean, Henri, Lapeyre, Bruno
The genome of Saccharomyces cerevisiae encodes three close homologues of the Escherichia coli 2′-O-rRNA methyltransferase FtsJ/RrmJ, designated Trm7p, Spb1p and Mrm2p. We present evidence that...
Droogmans, Louis, Roovers, Martine, Bujnicki, Janusz M., Tricot, Catherine, Hartsch, Thomas, Stalon, Victor, ...
N1-methyladenosine (m1A) is found at position 58 in the T-loop of many tRNAs. In yeast, the formation of this modified nucleoside is catalyzed by the essential tRNA (m1A58) methyltransferase, a...
A primordial RNA modification enzyme: the case of tRNA (m1A) methyltransferase
Roovers, Martine, Wouters, Johan, Bujnicki, Janusz M., Tricot, Catherine, Stalon, Victor, Grosjean, Henri, ...
The modified nucleoside 1-methyladenosine (m1A) is found in the T-loop of many tRNAs from organisms belonging to the three domains of life (Eukaryota, Bacteria, Archaea). In the T-loop of eukaryotic...
Purushothaman, Suresh K., Bujnicki, Janusz M., Grosjean, Henri, Lapeyre, Bruno
N2-Monomethylguanosine-10 (m2G10) and N2,N2-dimethylguanosine-26 (m22G26) are the only two guanosine modifications that have been detected in tRNA from nearly all archaea and eukaryotes but not in...
Urbonavičius, Jaunius, Skouloubris, Stéphane, Myllykallio, Hannu, Grosjean, Henri
Formation of 5-methyluridine (ribothymidine) at position 54 of the T-psi loop of tRNA is catalyzed by site-specific tRNA methyltransferases (tRNA:m5U-54 MTase). In all Eukarya and many Gram-negative...
MODOMICS: a database of RNA modification pathways
Dunin-Horkawicz, Stanislaw, Czerwoniec, Anna, Gajda, Michal J., Feder, Marcin, Grosjean, Henri, Bujnicki, Janusz M.
MODOMICS is the first comprehensive database resource for systems biology of RNA modification. It integrates information about the chemical structure of modified nucleosides, their localization in...
Marck, Christian, Grosjean, Henri
From 50 genomes of the three domains of life (7 eukarya, 13 archaea, and 30 bacteria), we extracted, analyzed, and compared over 4,000 sequences corresponding to cytoplasmic, nonorganellar tRNAs. For...
MARCK, CHRISTIAN, GROSJEAN, HENRI
Most introns of archaeal tRNA genes (tDNAs) are located in the anticodon loop, between nucleotides 37 and 38, the unique location of their eukaryotic counterparts. However, in several Archaea, mostly...
Marck, Christian, Kachouri-Lafond, Rym, Lafontaine, Ingrid, Westhof, Eric, Dujon, Bernard, Grosjean, Henri
We present the first comprehensive analysis of RNA polymerase III (Pol III) transcribed genes in ten yeast genomes. This set includes all tRNA genes (tDNA) and genes coding for SNR6 (U6), SNR52, SCR1...
THUMP from archaeal tRNA:m22G10 methyltransferase, a genuine autonomously folding domain
Gabant, Guillaume, Auxilien, Sylvie, Tuszynska, Irina, Locard, Marie, Gajda, Michal J., Chaussinand, Guylaine, ...
The tRNA:m22G10 methyltransferase of Pyrococus abyssi (PAB1283, a member of COG1041) catalyzes the N2,N2-dimethylation of guanosine at position 10 in tRNA. Boundaries of its THUMP (THioUridine...
Formation of the conserved pseudouridine at position 55 in archaeal tRNA
Roovers, Martine, Hale, Caryn, Tricot, Catherine, Terns, Michael P., Terns, Rebecca M., Grosjean, Henri, ...
Pseudouridine (Ψ) located at position 55 in tRNA is a nearly universally conserved RNA modification found in all three domains of life. This modification is catalyzed by TruB in bacteria and by Pus4...
Walbott, Hélène, Leulliot, Nicolas, Grosjean, Henri, Golinelli-Pimpaneau, Béatrice
The 5-methyluridine is invariably found at position 54 in the TΨC loop of tRNAs of most organisms. In Pyrococcus abyssi, its formation is catalyzed by the S-adenosyl-l-methionine-dependent tRNA...
MODOMICS: a database of RNA modification pathways. 2008 update
Czerwoniec, Anna, Dunin-Horkawicz, Stanislaw, Purta, Elzbieta, Kaminska, Katarzyna H., Kasprzak, Joanna M., Bujnicki, Janusz M., ...
MODOMICS, a database devoted to the systems biology of RNA modification, has been subjected to substantial improvements. It provides comprehensive information on the chemical structure of modified...